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:: Volume 24, Issue 2 (Summer 2024) ::
J Ardabil Univ Med Sci 2024, 24(2): 160-173 Back to browse issues page
Isolation and Identification of Toxoplasma gondii from Aborted Fetuses of Goats by PCR in Lorestan Province
Morad Beiranvand , Hossein Hamidinejat * , Somayeh Bahrami , Mohammad Reza Tabandeh , Meysam Makki
Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz ,Ahvaz, Iran , has.hamidinejat@gmail.com
Abstract:   (216 Views)
Background: A zoonotic obligate intracellular protozoan parasite, Toxoplasma gondii, infects all warm-blooded animals as well as humans worldwide. Identification of the level of infection in intermediate hosts gives us an important data about understanding the role of this parasite in human health as well as estimating the economic loss in livestock. Therefore, the main aim of this study was the isolation and identification of T. gondii from aborted goat fetuses by PCR in Lorestan province.
Methods: From autumn 2023 to summer 2024, the brain and liver of 100 goat fetuses were examined for T. gondii by PCR based on the amplification of 529 base pair fragments from repetitive regions of the parasite genome. The study was performed in three aborted fetus groups, less than 2 months, 2 to 4 months and more than 4 months.
Results: From a total of 100 examined samples, conventional PCR detected the T. gondii infection in 6 (6%) and 2 of the brain and liver fetuses respectively.
Conclusion: This study shows a notable level of infection in goat fetuses, and as a result, T. gondii should be considered an important agent involved in the abortion of goats in the Lorestan province of Iran. On the other side, it is necessary to pay more attention to the risk of transmission of this parasite from farmed animals to humans, especially pregnant women and people with suppressed immune system.

 
Keywords: Toxoplasma gondii, Zoonoses, Abortion, PCR, Iran
Full-Text [PDF 964 kb]   (132 Downloads)    
Type of Study: article | Subject: Parasitology-Mycology-Entomology
Received: 2024/09/26 | Accepted: 2024/11/2 | Published: 2024/07/20
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Beiranvand M, Hamidinejat H, Bahrami S, Tabandeh M R, Makki M. Isolation and Identification of Toxoplasma gondii from Aborted Fetuses of Goats by PCR in Lorestan Province. J Ardabil Univ Med Sci 2024; 24 (2) :160-173
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Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Volume 24, Issue 2 (Summer 2024) Back to browse issues page
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