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The Effect of Cannabis Extract on SH-SY5Y Nerve Cell
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Farshid Parsa    , Ebrahim Hosseini * , Davood Mehrabani , Sara Hashemi |
| Department of Biology, Shiraz Branch, Islamic Azad University, Shiraz, Iran , Hosseini:ebrahhim.hossini@yahoo.cm |
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Abstract: (2761 Views) |
Introduction & objectives: Cannabis extract is an important psychoactive substance that have used by people around the world. Due to the diverse effects of cannabis on brain cells, this study was conducted to investigate the effect of cannabis extract on the growth of SH-SY5Y neurons.
Methods: In this experimental study, SH-SY5Y cells were prepared from Pasteur Institute of Iran and then incubated in DMEM, supplemented with 10% fetal bovine serum ,embryo-L glutamine, penicillin and streptomycin at 37oC and 5% CO2. following cell proliferation, the cells in the fourth passage were divided into control and experimental groups which were treated with cannabis at concentrations of 100 and1000 ng/ml. during 1 to 9 days, SH-SY5Y cells growth was calculated by flow cytometry using PDT= T× formula.
Results: SH-SY5Y cells adhered to the bottom of the flask 24 hours after transfer to the cell culture flask. These cells were initially spherical and after 24 hours become spindle. The results of cell count test on days 1 to 6 showed the growth of cannabis-treated cells similar to the control group, but from the sixth day, in the cannabis extract treated groups, a significant reduction in cell growth was observed at the level of p<0.05 compared to the control group.
Conclusion: The results showed that SH-SY5Y cells in the culture medium were spherically shaped, similar to fibroblast cells, and the based on the results of cell count, it was determined that cannabis inhibits the growth of these cells. |
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| Keywords: Cannabis, SH-SY5Y, Cell Growth |
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Full-Text [PDF 918 kb]
(1411 Downloads)
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Type of Study: article |
Subject:
Physiology
Received: 2020/05/27 | Accepted: 2021/01/29 | Published: 2021/01/29
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