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Showing 3 results for Toxoplasmosis
Ahmad Daryani , Mohsen Sagha ,
Volume 4, Issue 3 (9-2004)
Abstract
Background & Objectives: Infection with Toxoplasma gondii can cause severe illness when transmitting to fetus or when it is reactivated in immune-suppressed persons. The aim of this study was to determine the prevalence of antibodies against toxoplasma gondii in women referring to laboratory of health center for medical examinations before marriage. Methods: This descriptive cross-sectional study was performed on 504 samples collected from women in Arabil, Iran, in 2002. The samples were studied by indirect immunofluorescent assay (IFA) for determination of IgG and IgM antibodies to toxoplasma. Results: The seroprevalence of IgG antibody at a titer of ³ 1:20 was 34.7%. The highest antibody titer frequency was observed in 1:20 titer (11.7%) and the lowest belonged to 1:3200 (0.4%) and 1:6400 (0.4%) titers. 20 persons (4%) showed IgM antibody against Toxoplama gondii. No statistically significant differences were observed between the prevalence of antibodies on the one hand and age and history of contact with cat or domestic animals on the other. Conclusion: since 65.3% of these women in Ardabil were seronegative, health education is required to omit the risk factors, especially during the pregnancy.
Hosein Alimohammadi, Nasrin Fouladi , Firouz Amani , Mehran Safarzade, Farhad Purfarzi , Effat Mazaheri ,
Volume 8, Issue 4 (12-2008)
Abstract
Background & Objectives: Toxoplasmosis is an important zoonosis parasitical disease. Infection with Toxoplasma gondii in transmission of parasite to fetus or its reactivation among immune-compromised persons can lead to server clinical symptoms. The aim of study was to determine immune level of Toxoplasmosis among women who were to get married and have referred to Health Center of Ardabil. Methods: In this study 272 blood samples of women who had referred to health center of Ardabil in 2007 were collected. Samples were tested with ELISA method to detect Anti-Toxoplasma IgM and IgG Antibodies. Results: The tests showed that 42.3% of women were IgG Positive, 57.2% were IgG negative and 2.6% women were IgM positive and also IgG positive. They have ( IgG positive women) 11.3% family history of abortion, 96.5% eating raw vegetables, 92.2% eating half cooked meat, 89.6% not washing the vegetables with disinfectants, 29.6% animal contact, 57.4% contact with raw meat and 94.8% used piped water. Conclusion: Since 57.7% of women who were to marry in Ardabil city were negative from the view point of Toxoplasmosis, health education for elimination of risk factors especially within pregnancy period seems mandatory.
Khadijeh Khanaliha, Farah Bokharaei-Salim, Mohsen Sadeghi, Borna Salemi,
Volume 22, Issue 3 (10-2022)
Abstract
Background & objectives: Toxoplasma gondii is an obligate intracellular parasite with global distribution. Diagnosis of Toxoplasma gondii infection with high sensitivity and specificity is very important in managing and treating this disease. The purpose of this study is serological and molecular investigation of toxoplasmosis using B1 gene in HIV- positive patients referred to hospitals affiliated with Iran University of Medical Sciences.
Methods: In this study, 660 blood samples were collected from HIV/AIDS- positive patients referred to hospitals affiliated with Iran University of Medical Sciences. Patient samples were examined for the presence of IgG and IgM antibodies against Toxoplasma gondii using an ELISA kit. Genomic DNA was extracted from the patient's serum, as well as peripheral blood mononuclear cell (PBMC) and whole blood samples, and then Real time-PCR was performed.
Results: Although IgG antibody against Toxoplasma gondii was positive in 158 (23.9%) patients out of 660 HIV- positive patients, IgM antibody was positive in 5 (0.76%) patients. The results of Real-Time PCR showed that 7 (1.06%) patients were positive in PBMC samples, of which five patients were positive for IgM antibodies against Toxoplasma gondii while two patients had high- level Toxoplasma IgG antibody titers.
Conclusion: The results of the study indicate that the Real-time PCR method using PBMC DNA samples is a suitable method for the diagnosis of toxoplasmosis. This method, together with the antibody test, especially the high titer of Toxoplasma IgG antibodies, can be helpful in the diagnosis of toxoplasmosis.
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