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Showing 42 results for Tem
Darioush Savadi Oskoui , Maziyar Hashemilar ,
Volume 3, Issue 3 (9-2003)
Abstract
Temporal arthritis (TA) is a systemic inflammatory vasculitis with unknown etiology, which involves large and medium sized arteries. A wide spectrum of systemic, neurological and ophthalmological complications appears in the course of the disease the most serious of which is visual impairment leading to permanent blindness in 60% of the cases. TA is diagnosed through an elevated ESR (>60 mm/Hg) and pathological findings of arterial biopsy. It is considered as one of the urgent neurological cases in which the patient should be treated with corticostroid immediately after diagnosis. The subject of this research was a 62-year-old male who had had headache associated with fatigue, night sweats and low mood for four month. The diagnosis of TA was made considering an elevated ESR and temporal artery biopsy findings. Treatment with prednisolone was initiated and continued for 19 months resulting in complete recovery.
Daruoosh Savadi Oskoyi , Ali Abedi, Reza Khandaghi,
Volume 5, Issue 4 (12-2005)
Abstract
Background & Objectives: Hypokalemic paralysis is a relatively uncommon syndrome which is potentially dangerous. If it is diagnosed and treated timely, the patients will be saved without any side effects. Various diseases can lead to this syndrome which is characterized with decreasing of serum potassium and acute systemic paralysis. In hypokalemic paralysis, the serum level of potassium reaches to less than 3.5 Mmol/lit. This may be caused by transferrence of K to intracellular department, (periodic hypokalemic paralysis and periodic thyrotoxic paralysis). The aim of this study was to determine sex and age prevalence of these patients as well as other features of the patients suffering from periodic hypokalemic paralysis. Methods: 55 patients participated in this prospective, descriptive and cross-sectional study which was conducted from 1999 to 2003. These patients were referred to the neurological department with hypokalemic diagnosis on the basis of laboratory and clinical examinations. The data were collected using a questionnaire. Also the serum level of K was measured. The data were analyzed by SPSS software. Results: from 50 patients, 46 (92%) were male and others were female. The patients were in 19-60 age range (Mean=33.16 SD=9.1). Mean serum level of K+ was 2.37 Mmol/lit (SD= 0.49) in the first hospitalization. The incidence of hypokalemia increased with aging , but majority of the patients (76%) were in age range 20-40. In 86% of the patients the time of hypokalemia crisis was at night and early morning. 11 patients had a high consumption of rich carbohydrate foods previously and 11 others had consumed corticosteroids. There was relapse of disease in 21 patients. 40 patients had paralysis in 4 extremities and 12 patients in 2 extremities. 4% of the patients were hypertensive and 4 patients were hyperthyroid. EKG was normal in 8 patients but in other cases there were abnormal changes. Conclusion: Despite the international references, the incidence ratio of male to female is high but the decreasing of K is more severe in female. Asetazolamide and oral KCL therapy not only decrease the period of hospitalization, but also are more effective in treatment of the disease.
Shahnam Arshi, Seid Reza Majdzadeh, Homauoon Sadegi, Seid Hadi Sezavar, Zahra Tahmacian, Looeiz Amanati , Ali Ruhi,
Volume 6, Issue 1 (4-2006)
Abstract
Background & Objectives: Regarding the recent implementation of new information system in medical universities, it seems quite necessary to perform an evaluation study on its effectiveness as well as on the functioning of health care systems regarding the different parts of this system. The aim of this study was to investigate skills of family health personnel in health centers of Ardabil province on correctly completing two common data forms called family planning form and maternity care data form. This can be a reasonable criterion for how well the new system is working in the field of family health. Methods: This is a descriptive-analytical study. All family health care personnel from Ardabil province heal care centers were entered into the study. They were asked to enter data related to 10 case presentations into blank forms. After filling out the forms they were checked and evaluated for correctness and final scores were analyzed by SPSS. Results : From all the data collected from 9 cities of the province, 38.5% lacked an academic education and 52% were graduated in midwifery. Mean personnel skill was 4.38 out of 10 and only 11.7% had acceptable skills in filling out the forms. Those with academic education outperformed the non-academic ones. Discrepancies regarding the correct ways of filling the forms, were observed among different cities’ health care centers. Conclusion: The findings indicated family health personnel were not skillful enough in completing the data forms. This led to their weak and non-dependable statistic which in turn would waste budget and energy. Moreover, the validity of the collected data would be under question. So the form 108 is very complicated and needs revision.
Mohammad Narimani , Yoosof Khonya , Abbas Abolghasemi , Masoud Ganji , Gafar Majidi ,
Volume 6, Issue 2 (6-2006)
Abstract
Background & Objectives: Clinical findings show that stress can gradually affect the activity of various systems of the body such as immune system and impair them. Different variables potentiate and modify this effect. This research aimed at studying the relationship of personality traits (self-respect, tenacity, type A personality), stress, and stress coping skills with immune system status in nurses. Methods: In this descriptive-analytical study, 246 individuals were selected by simple random sampling method. At the first stage, the subjects on the job completed the questionnaires of self-respect, tenacity, type A personality, stress, and stress coping skills. At the second stage to determine the amount of antibodies, 2-cc blood of the subjects was assessed. The data were analyzed using correlational methods. Results: The findings indicated a significant, positive and meaningful relationship of immunoglobulin antibody with “self-respect”, and IgM antibody with “type A personality” in male nurses (p<0.05). But there was no significant relationship between the immune system variables and tenacity, stress, and stress coping skills. From among the predictive variables (self-respect, tenacity, type A personality, stress, and stress coping skills) only the stress variable had significant relationship with the criterion variable (IgA antibody)(p=0.05). Conclusion: Regarding the results it can be said that self-respect may adjust IgG variable in stress positions (especially in chronic stress). So in hard jobs such as nursing, psychological characteristics should be considered as an important job factor along with physical capabilities.
Mohammadghasem Golmohammadi , Hasan Azari , Mohammad Mardani , Ebrahim Esfandiari , Radnili Rietze ,
Volume 8, Issue 2 (6-2008)
Abstract
Background & Objective: It is now clear that the adult mammalian subventricular zone (SVZ) contains a population of neural stem cells (NSCs) that give rise to neurons and glia. Owing to their rarity, and a paucity of NSC-specific markers, the neurospher assay (NSA) is a common and selective method for isolating and understanding the biology of embryonic and adult neural stem cells. There are different methods for neurosphere growing from different regions of the CNS including Lateral ventricles. The objective of this study is introducing a new and effective strategy for more neurosphere firming from the SVZ of the adult mouse brain lateral ventricle using NSA. Methods: Two different methods were used in order to isolate and culture the SVZ of the lateral ventricles using NSA. In the first method (Ritze and Reynolds method) the rostral part of the SVZ of the lateral ventricles was dissected into single cell suspension and cultured using NSA. In the second method (vibratome resecting of the brain) after cutting the brain into 400 µm serial sections using a vibratom, the SVZ was microdissected from all sections of rostral part of lateral ventricle and cultured separately, using NSA. Primary neurospheres were counted seven days after plating. Then the mean numbers of neurospheres generated in two different methods were compared. Results: The mean number of neurospheres generated by sectioning method was much higher than the one generated using first method (P<0.0001). The distribution and frequency of neurosphere forming cells (or NSCs) is not the same along the antero-posterior axis of the rostral part of the lateral ventricle. The greatest frequency of neurosphere forming cells was detected in 0.74mm rostral to the bregma. Conclusion: Second section method, due to more neurosphere generation, in comparison with the first method is more appropriate and efficient for neurosphere forming from the SVZ of the lateral ventricle.
Jabraeil Farzi, Parviz Salem Safi, Alireza Zohoor, Farbod Ebadi Fard Azar,
Volume 8, Issue 3 (9-2008)
Abstract
Background & Objectives: In addition to the socio-economics costs, Diabetes is a major cause of illness and death at all groups. Diabetes affects over %10 of adults in the world. Despite promising trend in improving many aspects of health Care and treatment in the last decade in our country, little attention has been paid to the subject of registering Diabetes on an international standard. Quality improvement of treatment, identifying high risk groups, controlling, preventing and evaluating of diabetes, would materialize only when the national Diabetes Registry System is established and its data are collected and completed on time. The aim of this study was to suggest Diabetes registry system in Iran, to meet the health and medical needs. Methods: This Comparative Cross-sectional study was conducted to evaluate the developed national diabetes registry systems in 2003. In this study national diabetes registry system in Britain and USA was evaluated by library resources, Websites and Email communication with internal and external specialist. Suggestions were made on the basis of economic cultural and geographic situations. These axes included aims, structures, data elements, registration, data collection processing, and classification and control of quality of system. Results: The results show that in order to optimize the diabetes registry system, structure, data elements, information gathering process, main goals, registration criteria, Classification systems and quality control mechanism of the current national diabetes registry system should be reevaluated. Conclusion: Considering the results and international diabetes institutes recommendation, the decrease in poor registry and increase in quality can be the advantages of model suggestion in comparison with the country's current system.
Masoud Maleki , Kazem Parivar , Mohammad Nabiyouni , Parichehr Yaghmaei , Mohammad Naji ,
Volume 9, Issue 2 (6-2009)
Abstract
Background and Objectives:Stem cells are characterized by the ability to renew themselves through mitotic cell division and differentiating into a diverse range of specific cell types. Wharton's jelly is the appropriate source of mesenchymal stem cells (MSCs) that have high differentiation competence. The aim of this study was differentiation of MSCs to lens fiber cells. In differentiation pathway of lens fiber cells, crystallins are expressed . Thus, crystallins can be used as differentiation marker of lens fiber cells. Method: In the current study MSCs were isolated from the mouse umbilical cord. It was minced into 1-2 mm3 fragments and then were incubated with collagenase type IA following pipetting for mechanical isolation of cells. Cell suspension was plated in 25 cm2 culture flasks. Alkaline Phosphatase detection kit was used for staining of undifferentiated UC-MSCs from passage I. In the experimental group MSCs were plated in the maintenance medium supplemented with bovine vitreous body (1:3 v/v) for induction. Protein lysate were prepared from cells on days 10 of induction and were analyzed on polyacrylamide gels and transferred to nitrocellulose membranes. Rat lens extract was used as a positive control. Anti-alpha A, alpha B crystallin, secondary antibody, vectastain ABC- kit (standard) and vector blue alkaline phosphatase substrate kit III were used. Results: Mouse umbilical cord MSCs had alkaline phosphatase activity. Morphological studies and separation of proteins in electrophoresis indicated that experimental group cells might probably differentiated into lens fiber cells. Conclusion: Mouse umbilical cord could be used as an appropriate source for MSCs. MSCs had fibroblast- like morph and experimental group indicated the presence of fiber-like cells that were long, thin, and parallel aligned. Electrophoresis and Western blot analysis showed there was a detectable expression of early developmental marker of lens fiber cells differentiation in experimental group.
Marjan Akhavan Amjadi , Faraz Mojab, Samira Shagbazzadegan,
Volume 9, Issue 3 (9-2009)
Abstract
Background & Objectives:Primary Dysmenorrhea (PD) is common gynecology problem in women. It is defined as a menstrual pain that occur in absence of pelvic pathology. PD is common reason of women and girls’ absence from schoole. Pain is main complain of PD but other systemic symptoms like headache, nausea and vomiting, diarrhea, fatigue, irritability and dizziness are common. Many chemical drugs have been used to cure dysmenorrheal, but each has its own side effects. Cinnamomum zeylanicum has been used as an anti-spasmodic agent in traditional medicine . Methods : This study was a placebo-control, triple blind and randomize clinical trial. We research. The efficacy of C. zeylanicum on severity of symptoms with PD Severity of symptoms were assessed. Data were collected by question validated questionnaires. The subjects of study were 47 single women, who sufrred from primay dysmenorrhes, They were randomly selected and their age use between 18-30 years old. We divided them two group, 26 case women in (treatment group) whom needed capsules contain C. zeylanicum and control group whom revided placebo capsules. administered as soon as onset of pain feeling or bleeding, five capsule a day and continued for minimum three days. Severity of systematic symptoms was assessed with Andersch & Milsom verbal multidimensional scoring system. Data were analyzed through by SPSS software. Results: The mean age of women in case and control groups were 20.69 ± 1.93 and 21.7+4.6. There was a significant differences (p<0.001) in the Severity of pain between two groups. The severity of all the systematic symptoms decreased in both groups, but this reduction was not significant between two groups. No significant difference was seen for systematic symptoms between twogroups. In this study, had not been observed any side effects by using of C. zeylanicum . Conclusion: The results our study should that C. zeylanicumcan be effective in reducing the severity of dysmenorrheal, but C. zeylanicum has no sufficient effect on relief of systematic symptoms accompanying with dysmenorrhea.
Babak Mostafazadeh, Esmaeil Farzaneh,
Volume 10, Issue 1 (4-2010)
Abstract
Background & Objectives : One of the limitations in management of patients suffering from decreased level of consciousness due to drug poisoning is the inability to interview the patient. The aim of the study was to compare characteristics of the two subgroups of these patients those who had a suicidal attempt history with others. Methods: In this cross-sectional study, 19 patients with suicidal attempt history and 66 patients without this history were selected among patients who were admitted for drug poisoning to referral poisoning hospitals in Tehran, Iran, in August, September and October 2009. All of them had decreased level of consciousness due to unknown drug poisoning. Demographics, poisoning characteristics, past medical history and substance use history of the patients were recorded. Results: Different between previous history of suicide action with drug material decreased level of consciousness caused by poisoning, was significantly (p=0.04). Mental disease (p > 0.01), number of tablets consumed (p=0.02), interval between drug consumption and emergency forces to reach the place (p= 0.021), described in deliberate self poisoning patients (Self Poisoning) (p=0.001), decreased level of consciousness grade (p=0.046), U.S. fail initial level of consciousness after emergency measures (p=0.02) and the need for health care and advanced next (p= 0.013) in patients with decreased level of consciousness with a history of previous suicide compared with other group were higher. Significant difference between the two age groups (P> 0.05), gender and history of illegal drug use (P> 0.05) did not exist. Conclusion: According to these findings, when dealing with patients suffering from decreased level of consciousness due to unknown drug poisoning, paying attention to history of suicidal attempts in these patients can help medical professionals in determining more effective treatment options for them. Medical staff should also record previous suicide as a risk factor in the risk assessment to consider the disease.
Abbasali Imani Foolad , Zahra Rostami , Reza Shapouri,
Volume 10, Issue 3 (9-2010)
Abstract
Background and Objectives: Detection of TEM and SHV genes in ESBL producing Pseudomonas aeruginosa and their antimicrobial resistance pattern can provide useful information about the epidemiology and risk factors of associated infections. In this study we determined the antimicrobial susceptibility pattern and prevalence of ESBLs in clinical isolates of Pseudomonas aeruginosa by phenotypic and genotypic methods. Methods: In this analytic-descriptive study, 110 Pseudomonas aeruginosa strains isolated from different clinical specimens were used. The pattern of antimicrobial resistance was determined by disk diffusion (Kirby-buer) method. The ESBL production was determined by combination disk method using disks containing ceftazidim and cefotaxim alone and in combination with Clavulanic acid. SHV and TEM types of ESBL producing genes was detected by PCR. Results: In this study Co-trimoxazole and Amoxicilin with 96.4% and 92.7% and Amikacin with 17.3% showed the highest and lowest resistance against isolates respectively. According to PCR results 37.5% and 12.5% of isolate were carried SHV and TEM genes respectively and 12.5% of isolate were carried both the SHV and TEM genes. Conclusion: According to the results most of the isolates are drug resistant and among the ESBL producing strains the frequency of SHV type is higher than TEM . The isolate ceftazidim resistance was contains SHV (37.5%) and TEM gene (12.5%), that showed SHV and TEM genes play more important role in create of ceftazidim resistance than cefotaxim resistance.
Farshad Sheikh Akbari , Bita Shahbazzadegan, Mahdi Samadzadeh, Hosein Ghamary Givi, Mohammad Narimani ,
Volume 11, Issue 2 (6-2011)
Abstract
Background & objectives: Exact diagnosis of mental disorder is important in psychology. With respect to common symptoms in schizophrenia, schizoaffective and bipolar mood, correct diagnosis of these disorders seems to be very difficult. The main goal of this study was to compare the body temperature of schizophrenic, schizoaffective and bipolar mood patients. Method: In this descriptive study, 60 schizophrenia, schizoaffective and bipolar mood patients, 20 persons in each group were selected. The patients were interviewed and their body temperature was measured. Finally, the data were analyzed by one-way ANOVA. Results: Schizophrenia, schizoaffective, bipolar mood patients had 38.15 ±11.13, 34.8±10.99, and 35.25 ± 9.05 years as a mean, respectively. About 63.3% of individuals had secondary , 15% high school, 16.7% diploma and 5% undergraduate education . About 51.7% of patients had non-governmental job, 8.3% and 40% were employed and jobless, respectively. Among them 45% were single and the rest married. 15% of individuals were admitted once, 25%, twice, 3.3% three times, 20% between 4-10 times and 41.7% frequently. Statistically, there was significant difference between the mean of body temperature in schizophrenia, schizoaffective and bipolar mood patients, (p < 0.001). Conclusion: The difference between the body temperature mean in three groups of patients showed that schizoaffective patients were more close to bipolar mood patients, and schizophrenia patients differ in terms of base keeping mean and body temperature balance from two other groups (schizoaffective and bipolar mood).
Mohammad Ghasem Golmohammadi , Mohsen Sagha , Hasan Azari , Norouz Najafzadeh,
Volume 11, Issue 3 (9-2011)
Abstract
Background &Objectives: Stem cells are a class of undifferentiated cells that are able to differentiate into specialized cell types. The discovery of such cells in the adult mammalian central nervous system (CNS), an organ traditionally thought to have little or no regenerative capacity, opened the door to treatment of degenerative diseases of CNS like Stroke, Parkinson, Alzheimer and Spinal Cord Injury. Thus, here we described the isolation of neural stem cells from the adult mouse brain using the neurosphere assay (NSA) and differentiation of these cells to neural adult cells in details. Methods: The rostral part of the subventricular zone (SVZ) of the lateral ventricles in the adult mice was dissociated into single cell suspension and cultured using NSA. Primary neurospheres were counted seven days after plating and then the mean number of neurospheres was recorded. The differentiation of neural stem cells into adult neural cells was accomplished by plating the neurosphere-derived cells in differentiating media. Immunocytochemistry and specific markers were used for the identification of the adult neural stem cells. Results : The cell suspension obtained from the rostral part of the SVZ of the lateral ventricles generated multipotential colonies, called neurospheres, 7 to 10 days post- incubation. The mean number of neurospheres generated from SVZ was 505±62. The multipotentiality of the neurospheres was shown by palting them in differentiating media and generating adult neural cells including neuron, astrocyte and oligodendrocyte . Conclusion: Owing to their rarity and paucity of neural stem cell specific markers, the NSA is a common and selective method for isolating and understanding the biology of embryonic and adult neural stem cells.
Adalat Hoseinian , Farhad Pourfarzi, Nasrin Sepahvand , Shahram Habibzadeh, Behzad Babapour , Hosein Doostkami , Nasrin Fouladi, Mehri Seyed Javadi ,
Volume 12, Issue 1 (4-2012)
Abstract
Background & Objectives: Myocardial infarction is one of the most common causes of mortality throughout the world. Utilization of thrombolytic drugs at the first hours upon MI leads to decrease in the rate of mortality. Streptokinase (SK) is commonly used because of its cost and availability. The golden time for maximum effect of SK appeared to be first hours after MI. The drug efficiency diminishes after 3 hours and it will be ineffective after 12 hours. This study was aimed to investigate interval between onset of the clinical symptoms and streptokinase receiving in patients with acute myocardial infarctionadmitted for drug reception. Methods: This cross-sectional study was conducted in 150 patients with STEMI (ST elevation Myocardial Infarction ) who were attended to emergency room of Imam Khomeini hospital and fulfilled a questionnaire containing the data about age, sex, educational level, onset of symptoms, referring time to emergency room, SK utilization, past medical history of cardiac diseases, type and location of AMI, contraindications and side effects of SK. Results: In 111 men (74%) and 39 women (26%), the mean for interval between onset of symptoms and drug injection was 8 hour and 27 minutes ± 8 hour and 18 minutes .Only 41% of patients received streptokinase in less than 3 hours after chest pain. The main reasons for delaying in drug consumption were delayed decision to come to hospital in 61% of persons, long distance to hospital for 22% of individuals, delayed admission tor CCU for 13% of cases and delay in physician diagnosis for 0.043% of patients. To have a male sex and residency in city where the hospital was establilished were associated with less-delayed treatment start. However, the educational level, age and history of MI were not correlated with onset of treatment. Conclusions: Our results clearly show that the majority of patients receipt streptokinase more than 3 hours after chest pain. With considering the factors associated with delay to receive SK, teaching the patients with ischemic heart disease and giving information about golden time for thrombolytic therapy and its effect in prognosis as well as extending cardiac disease treatment centers in suburbs are recommended.
Saber Zahri, Masoud Maleki, Kamaladdin Hamidi , Seiyeh Mahsa Khatami ,
Volume 13, Issue 1 (4-2013)
Abstract
Background & Objectives: Stem cells are fundamental supporter of multicellular tissue. They allow blood, bone, gametes, epithelia, nervous system, muscle, and other tissues to be replaced by fresh cells throughout life. In recent years human Wharton’s jelly stem cells (WJSCs) have gained attention. They express a number of surface markers characteristic of mesenchymal stem cells. In this study, human Wharton’s jelly stem cells were isolated using explant method. To show the stemness property of these cells, three CD markers including CD105, CD44 and CD34 were tested. Methods: The umbilical cord samples were collected by Caesarian section at Arta Hospital in Ardabil. Cords were transferred in sterile conditions and stem cells were isolated using explant method. After log phase, cells were passaged then growth characteristics and CD105, CD44 and CD34 markers investigated by RT-PCR. Results: Separation of human Wharton’s jelly stem cells were started after 7 days. WJSCs in culture revealed two distinct cell population named Type 1 and Type 2. RT-PCR results showed that WJSCs were CD105+, CD44+ and CD34-. Conclusion: Human umbilical cord stem cells could be an alternative source instead bone marrow stem cells for cell therapy and tissue engineering. These cells have a fibroblastic appearance. Following the lag phase and into log phase respectively, cells grow easily in culture and retain stemness properties in higher passages.
Hosein Najafzadeh, Mahmood Khaksari Mahabadi, Sareh Rezaie, Peghah Ardalani,
Volume 13, Issue 1 (4-2013)
Abstract
Background: Crocus sativus L., commonly known as saffron is used for different purposes such as an antispasmodic and expectorant. Recent studies have demonstrated that saffron extracts have antitumor, radical scavenger, hypolipidemic, anticonvulsant effects and it improves activity on learning and memory. The aim of the present study was to assess the effect ofaqueous saffron extracton weight, length, macroscopic and microscopic skeletal malformation in fetuses of rats. Methods: Study was carried on 3 groups of female Wistar rats. Group1 was kept as control and received normal saline with equal volume of extract Group 2 received saffron extract with 80mg/kg-IP on 10th and 11th day of gestation Group 3 received saffron extract with 200mg/kg-IP on 10th and 11th day of gestation. Rats were dissected on day 20 of gestation, embryos harvested by cesarean section. The living status and weight and length of fetuses were detected. After examination for determination of gross malformations, fetuses were placed in alcohol and stained by Alizarin red-Alcian blue method then evaluation was carried by stereomicroscope. Results: Saffron extract at dose of 80 mg/kg significantly increased the weights and body-lengths of fetuses in compare with saline and extract at dose 200 mg/kg. Macroscopic and Microscopic studies did not show any skeletal abnormalities by two above doses of saffron. Conclusion: The results show short time administration of saffron aqueous extract does not cause malformation on skeletal system.
Mohammadreza Behvarz , Masoud Maleki , Mohammadreza Mashayekhi ,
Volume 14, Issue 4 (1-2014)
Abstract
Background & objectives: Adult stem cells are undifferentiated cells that replace dead or injured cells. There are adult stem cells in some regions of human tissues and hair follicle is one of the tissues that have adult stem cell source and these cells have an important role in hair life cycle. In this study, we investigated the isolation of hair follicle stem cells (HFSCs) and expression of mesenchymal stem cell markers on the isolated cells. Methods : Human hair follicles obtained from men scalp tissue by micro punch technique. Hair follicles isolated and cultured in culture flasks in DMEM-F12 + FBS. After outgrowth of stem cells from hair bulges, they analyzed by flow cytometry for detection of stem cell markers. Results: 23 to 27 days after isolation and culture of HFSCs in uncoated cell culture flasks, cell surface markers expression studied by flow cytometry. Flow cytometric analysis showed 25.26% Stro-1, 50.85% CD90, 45.24% CD105, 61.20% CD44, 8.20% CD45, 11.86% CD146, 2.72% CD106, 7.21% CD166 and 26.74% CD19 expression in HFSCs. Conclusion: In this study, isolated stem cells significantly expressed some of the mesenchymal stem cell markers higher than other markers. These markers give certain characteristics to HFSCs, and introduce the cells as an alternative option for cell therapy, tissue engineering and regenerative medicine.
N Danesh Far , H Peeri Dogaheh, M Ghiamirad ,
Volume 15, Issue 2 (7-2015)
Abstract
Background & objectives: Resistant microbial strains are a serious threat to public health in different societies. A mong the extended-spectrum β-lactamases (ESBL) producing strains the Enterobacteriaceae family which is considered as the main factors producing urinary tract infections, have created many problems in treatment of this kind of infections. This study was conducted to determine the frequency of β-lactamase TEM-1 gene in Enterobacteriaceae isolated from urine samples in Ardabil city.
Methods: Within 6 months, 400 urinary isolates of Enterobacteriaceae of inpatients and outpatients were collected in Ardabil hospitals and were identified by standard methods. Antimicrobial susceptibility of isolates was tested by disk diffusion method, and ESBL producer confirmatory test was conducted using combined disk. Finally, the frequency of β-lactamase TEM-1 gene in producing extended-spectrum β-lactamases strains was investigated using PCR.
Results: From 400 isolates of Enterobacteriaceae, 150 cases (37.5%) were ESBL producing. PCR results showed presence of the TEM-1 gene in 69 cases (46%). The frequency of this gene in isolates of Enterobacter (Aerogenes, Cloacae), Klebsiella (Pneumoniae, Oxytoca) and E. coli was obtained to be 62.5%, 54.5% and 44.8%, respectively. Proteus mirabilis and Serratia marcescens strains were lacking these genotypes.
Conclusion: As regards the presence of TEM-1 gene, there is also increasing in other members of the Enterobacteriaceae family including Klebsiella and Enterobacter in addition to E. coli, therefore sufficient identification of this strains is necessary to prescribe the right medicine.
Sh Heydari Tajaddod, N Najafzadeh, M Mahdavi Rad, H Sheikhkanloui Milan, H Kalarestaghi, V Nejati,
Volume 15, Issue 2 (7-2015)
Abstract
Background & objectives: Hair follicle stem cells (HFSCs) are multipotent and various types of HFSCs were introduced. HFSCs separation using cell surface markers is one of the interesting strategies in the replacement of old methods. In this study, we used magnetic activating cell sorting (MACS) to separate HFSCs.
Methods: In this study, HFSCs were isolated from Balb/c mice and dissected under an invert microscopy, and bulge area isolated and the bulge cells cultivated about 14 days. The CD34 positive cells isolated using CD34 monoclonal antibody and magnetic activated cell sorting system (MACS), then the cells incubated in DMEM/F12 and 10% FBS. The CD34 positive cells counted using a neubauer slide and evaluated under a fluorescent microscopy.
Results: Here, we isolated CD34 positive cells using MACS and 12±1. 04% of HFSCs were CD34 positive and we found that, CD34 positive cells survived during 7 days cell culture in vitro.
Conclusion: The results show that MACS is useful to increasing density gradient of cells in vitro.
J Ataei , Sm Shamshirgaran, M Iranparvar Alamdari , Ar Safaeian,
Volume 15, Issue 2 (7-2015)
Abstract
Background & objectives: Successful implementation of diabetes care can reduce acute and chronic complications of diabetes. This study was conducted to evaluate the quality of care according to a care scoring system among people with type 2 diabetes referring to the diabetes clinic in Ardabil.
Methods: This cross sectional study was conducted in 2014 and a total of 300 people with type 2 diabetes in age range of 20-70 years old were included via convenience sampling. Data were collected by an interviewer using a structured questionnaire and checklist. Each patient was classified according to a quality of care scoring system into 3 categories: ≤10, 15-20, >20. Statistical analysis performed by SPSS v.20 software and descriptive analysis used to display data as frequency tables and graphs. Analytical tests such as ANOVA, Kruskal-Wallis, Chi squared, and fisher exact test were also used to analyze the data.
Results: The mean age of patients was 54.13 ± 9.13 and the majority of them were women (72.3%). The mean of BMI and HbA1c was 29.58 ± 4.77 and 8.93± 2.13, respectively. The mean of FBS was 191.18 ± 84.23 and the average length of disease was 7.74±5.89 years. The mean of quality of care score among participants was 23.20±9.99. About one-third of patients (29.7%) had a quality care score ≤10, half of them (51%) had a score between 15-20, and only 19.35% had a score >20. There was an association between HbA1c, FBS, diastolic blood pressure, cholesterol and LDL with a quality of care score which was statistically significant (p<0.05).
Conclusion: In this study there was a gap between the current quality of care of patients and recommended standards. Therefore in order to minimize the current barriers and problems it is recommended to implement an effective interventional program. In addition, using a quality care scoring system is a rapid, easy and valid method for evaluating diabetes quality of care.
Zahra Kiasalari, Mehrdad Roghani, Tourandokht Baluchnejadmojarad, Athar Abdolrazaghnezhad,
Volume 16, Issue 1 (4-2016)
Abstract
Background & objectives: Temporal lobe epilepsy is associated with neuronal apoptosis. Curcumin has antioxidant and anticonvulsant activities, therefore this study was conducted to assess involvement of Bax and Bcl2 in protective effect of curcumin in epileptic rats.
Methods: 28 rats were divided into sham, curcumin-pretreated sham, epileptic (kainate), and curcumin-pretreated epileptic groups. Experimental model of epilepsy was induced by intrahippocampal administration of kainic acid. Rats received curcumin at a dose of 100 mg/kg. Finally, Nissl staining and Bax and Bcl2 immunohistochemistry were conducted on hippocampal sections and data were analyzed using one-way ANOVA and unpaired t-test. The p-value less than 0.05was considered statistically significant.
Results: Induction of epilepsy was followed by a significant seizure and curcumin pretreatment significantly reduced seizure intensity (p<0.01). In addition, there were no significant differences between the groups in Nissl staining of CA3 area neurons. In addition, Bax positive neurons were observed in CA3 area in kainate group and significantly decreased in curcumin pretreated rats (p<0.05). Meanwhile, Bcl2 positive neurons were also moderately observed in kainate group and curcumin pretreatment significantly increased it (p<0.05).
Conclusion: Curcumin pretreatment exhibits anticonvulsant activity in epileptic rats. It also decreases the expression of pro-apoptotic protein Bax and significantly enhances the expression of anti-apoptotic protein Bcl2 and hence could reduce neuronal apoptosis.
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