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Showing 3 results for Sperm Parameters
Mina Kiani, Paria Parto ,
Volume 17, Issue 4 (1-2017)
Abstract
Background & objectives: The present study examined the gonadotoxic effects of ciprofloxacin antibiotics and protective effects of L-carnitine.
Methods: In this study, 20 NMRI mice were divided into four groups: control, ciprofloxacin, L-carnitine, L-carnitine-ciprofloxacin (each group included 5 animals). The control group received normal saline, the treatment group 1 received 12.5 mg/kg ciprofloxacin, the treatment group 2 received 100 mg/kg L- carnitine and the treatment group 3 received 100 mg/kg L- carnitine and 12.5 mg / kg ciprofloxacin simultaneously. All animals were treated by intraperitoneal administration for 15 days. Testis and epididymis were collected to evaluate sperm parameters (sperm count, motility, morphology and viability).
Results: In the ciprofloxacin group, a significant decrease in sperm count, sperm viability, sperm motility, progressive sperm motility and normal sperm as well as a significant increase in rotating sperm motility, sperm without movement, head and tail abnormalities were observed (p≤0.05). A significant increase in sperm count, sperm survival, motility and progressive sperm motility, normal sperm and a significant decrease in rotating sperm motility, sperm without movement and head abnormalities in the L-carnitine-ciprofloxacin group were observed (p≤0.05); however, the reduction in tail abnormalities of sperm was not significant.
Conclusion: L-carnitine improves sperm parameters in the adult mice treated with ciprofloxacin.
Ali Soleimanzadeh, Ali Shalizar Jalali, Abdulrahim Abdullahi, Mohammad Moein Sabzeie,
Volume 20, Issue 2 (7-2020)
Abstract
Background & objectives: The present study investigated the effects of royal jelly on lead acetate induced toxicity on sperm parameters, reproductive hormone assay, and bak gene expression in NMRI male mice.
Methods: In this study, fifty four male mice were randomly divided into nine groups: control group (without royal jelly) (n=6); sham group(10 ml normal saline) (n=6); lead group (1000 ppm, oral) (n=6); Group 4: royal jelly (100 mg/kg/day, oral) (n=6); Group 5: royal jelly (250 mg/kg/day, oral)(n=6); Group 6: royal jelly (500 mg/kg/day, oral)(n=6); Group 7: royal jelly (100 mg/kg/day, oral) + 1000 ppm lead (n=6); Group 8: royal jelly (250 mg/kg/day, oral) + 1000 ppm lead (n=6) and Group 9: royal jelly (500 mg/kg/day, oral) + 1000 ppm lead (n=6). On day 35, blood samples were collected from anaesthetized mice by cardiac puncture to assess reproductive hormones and the testes were harvested for determination of sperm parameters and expression bak gene. Sperm parameters including motility, viability, DNA damage, morphology and total antioxidant capacity (TAC) levels were determined.
Results: The results showed that administration of royal jelly significantly enhanced sperm parameters and all reproductive hormone levels compared to control mice, (p<0.05). Also, treatment with lead acetate caused a significant reduction in levels of all reproductive hormones and a significant diminution in sperm motility, morphology, viability; with an increase in percentage of dead spermatocytes (p<0.05). The co-administration of the 250 and 500 mg/kg/day royal jelly with lead acetate could ameliorate the deleterious effects of lead acetate resulting in a significant increase in sperm parameters and all reproductive hormones and increase the total antioxidant capacity (TAC) levels (p<0.05). Also, the expression of bak gene in all treated (sham, royal jelly groups) and control groups was significantly lower than the lead acetate group (p<0.05).
Conclusion: In conclusion, our findings suggest that the royal jelly has a beneficial effect on male reproductive parameters following lead acetate induced toxicity in mice.
Azam Shiasi, Ramesh Monajemi, Marzieh Tavalaee, Mohammad Hossein Nasr Esfahani,
Volume 24, Issue 3 (10-2024)
Abstract
Background: Varicocele is one of the common causes of male infertility, associated with increased oxidative stress and damage to sperm DNA. This study aims to investigate the effects of L-cysteine, taurine, and vitamin B6 (LTB) on spermatogenesis, sperm function, and enzymes involved in the trans-sulfuration pathway in the testes of varicocele-induced rats.
Methods: Male Wistar rats (180–220 g) were randomly divided into five experimental groups: the control group (no intervention), the sham group (simple laparotomy), the varicocele group (V), the LTB-treated control group, and the LTB-treated varicocele group. Evaluations included sperm parameter assessments, testicular histology analysis, measurement of testosterone levels, vitamin B6 levels, various amino acids, and the expression of enzymes in the trans-sulfuration pathway at the protein level using the Western blot method.
Results: The study revealed a significant reduction in sperm quality parameters and sperm function (p<0.05) in the varicocele group compared to the control and sham groups. Additionally, enzymes involved in the trans-sulfuration pathway were reduced in the varicocele group compared to the sham group, although the difference was not statistically significant. Plasma B6 levels in the varicocele group showed a significant decrease compared to the control group (p<0.05). Supplementation with LTB improved the negative effects of varicocele on spermatogenesis and sperm function.
Conclusion: Antioxidants may play a role in improving varicocele. Using amino acids and vitamins involved in the one-carbon cycle and the trans-sulfuration pathway can enhance the testicular antioxidant system and counteract the negative effects of varicocele. These findings highlight the need for further studies and the potential for higher doses or longer durations of antioxidant supplementation to achieve better therapeutic effects.
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