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Showing 10 results for Sperm
Mokhtar Mokhtari , Mehrdad Shariatie , Nazanin Tadayon ,
Volume 7, Issue 1 (4-2007)
Abstract
Background & Objectives: Spironolactone is a diuretic and antiandrogenic drug and is used in the treatment of hypertention 'secondary hyprealdosteronism congestive heart failure' cirrhosis of the liver, nephrotic syndrome 'androgenic alopecia' gynecomastia and hirsutism. In this research, the effects of spironolactone on the serum LH, FSH, testosterone, dihydrotestosterone, and changes in body weight and testicular tissue in adult male rats, were studied. Methods: For this purpose 190 10 g male wistar rats (n=40) were randomly divided into the following grups: control, sham operated (received water) and 25, 50, 100 mg/kg oral spironolactone treated groups. After 14 days body weight and testis weight under laboratory methods, were measured and blood samples were taken from heart and used for the measuring of LH/FSH/testosterone and dihydrotestosterone and then the rates' testes, in order to evaluate the histological changes, were removed and weighed and after obtaining tissue section and staining through HE, they were studied. Results: Serum LH level showed a significant increase and testosterone and dihydrotestosterone levels showed asignigicant decrease in 100mg/kg spironolactone treated group ( p 0.05 ) and there was no significant difference among serum FSH level, body weight and testicular weight as compared to control group. Conclusion: It can be concluded that oral administration of spironolactone maximum dose for 14 days could increase serum LHlevel and decrease testosterone and dihydrotestosterone levels.
Kamaladdin Hassanzadeh , Parisa Yavari Kia , Vahid Emdady, Seyedkazem Maden ,
Volume 10, Issue 3 (9-2010)
Abstract
Background and Objectives: Three major semen parameters that involves on male infertility are sperm count, motility and morphology. In 50% of couples referring to physicians, because of infertility, male factor, solely or accompanying with female factor is responsible of infertility. Some of drugs are used to improve the semen parameters. The goal of this study was to study the effect of pentoxifylline on semen parameters (count, motility, morphology, volume of semen). Methods: Sixty one patients with impairment in semen parameters were selected and semen samples requested from each participant for analysis before treatment. Then patients were treated with pentoxifyllin, 400 mg three times a day, for three months. After that we took semen specimens again and analyzed them. Results: Mean total sperm count in case group before treatment with PF was (17 millions) and after treatment with PF reached to (21 millions). In 68.9% of patient, Sperm count increased (p<0.001) Mean motile sperm percent in case group before treatment with PF was 19.42% and After treatment with PF reached to 28.78% and 88.52% of patient this parameter increased (p<0.001). Parameter motility showed the most important after treatment. Mean normal morphology of sperm percent in case group before treatment with PF was 24%and after treatment with PF reached to 26.39% and in 32.8% of patient this parameter increased (p<0.001).Mean semen volume in this group before treament with PF was 1.95ml and after treament with PF reached to 1.93 ml. There was no significant variation in semen volume after treament with PF. (p=0.321) Conclusion: Results show that Pentoxifylline has significant efficacy for increasing sperm count, motility and morphology correction (specially for sperm motility). Considering that oral PF is safe and cheap, with easy application, we can use it for improving semen parameter’s quality before performance ART.
Masoumeh Hajishafiha, Shaker Salari Lac , Minoo Khairi Tabar , Siyamak Naji, Mahzad Sadaghiani , Nahideh Asadi ,
Volume 11, Issue 1 (4-2011)
Abstract
Background & objectives : Today there is a significant progress in the treatment of female infertility but there is no main improvement for the rate of implantation and live birth. This is because of non-implantation and early abortion that lead to decrease the rate of live birth. Genital infections such as bacterial vaginosis are common cause of this problem. Distinction and treatment of bacterial vaginosis is easy and non-expensive. Treatment of bacterial vaginosis could results in improving the rate of implantation and then live birth. Methods: We considered 209 infertile women treated with ICSI (intra cytoplasmic sperm injection). This study was performed in infertility clinic of KOSAR hospital affiliated to Urmia University of Medical Sciences, Iran. Before transferring of embryo, a sample was taken from posterior culdesac secretions by sterile cotton swap and fixed it on lamella. Then bacterial vaginitis was graded by a pathologist. The relationship of bacterial vaginosis with implantation and early abortion was studied. Data were entered into SPSS software and analyzed by t-test and Chi-Square test. p<0.05 is considered statistically significant. Results: Bacterial vaginosis was significantly more frequent in patients with tubal and ovulatory disorder (p=0.013). In women undergoing ICSI, bacterial vaginosis was not associated with decreased conception rate (p=0.892) and with increased rate of early pregnancy loss (p=0.44). Conclution: Bacterial vaginosis is prevalent in women with infertility. It is also the most important cause of infertility in patients with tubal and ovulatory disorder. Bacterial vaginosis does not affect fertilization rate.
Amirabbas Rashidi, Piraste Norouzi, Hamid Kalalianmoghaddam, Mehdi Khaksari, Mehdi Bagheri,
Volume 16, Issue 1 (4-2016)
Abstract
Background & objectives: Diabetes mellitus mediated by oxidative stress, creates serious metabolic disorders in testis. Kudzu root with an isoflavonin and saponin contents is often used as antidiabetic and antioxidant. This study aimed at preventing the oxidative effects of diabetes using Kudzu root.
Methods: In this study, 32 male Wistar rats were randomly selected and divided into four groups: control, diabetic and diabetic rats treated with Kudzu 50 and100 mg/kg. Diabetes was induced by intraperitoneal injection of 55mg/kg streptozocin. One week after injection, the rats started to receive Kudzu at the doses of 50 and 100 mg/kg for five weeks by gavage. Testicular damage was examined by using hematoxylin-eosin staining protocol. Hormonal and blood biochemical factors were measured.
Results: The results of this study showed that diabetes causes a high blood sugar levels and reduces the spermatogonia and Sertoli cells with decreased spermatogenesis,sperm count and function. These effects were improved in the treatment groups. Decrease in blood sugar and increase in the number and motility of sperm cells and spermatogonic cells were also observed together with enhanced seminiferous tubule diameter and lower basement membrane thickness.
Conclusion: Kudzu with the ability to reduce blood sugar, improves diabetic-induced testicular damage and can have a therapeutic role in diabetes.v
Eslam Zabihi, Seeyedeghbal Motavalli Bashi , Pirooz Pourmohammad, Ali Abedi ,
Volume 17, Issue 2 (7-2017)
Abstract
Background & objectives: Terfezia Boudieri (TB) has been used as a sexual stimulant for men in traditional medicine. TB containing fatty acid, flavonoids, beta-carotene, minerals and antioxidants such as, catechin, therefore, this study aimed to investigate the effects of the hydroalcoholic extract of Terfezia Boudieri on sperm and testosterone levels in male rats.
Methods: In this study, 21 adult male Wistar rats, each weighing approximately 250±20g, were randomly divided into three groups (n=7). The first group (control) with no treatment, the second group (sham group) received normal saline (extract solvent) and the third group, was injected intraperitoneally (IP) with 105 mg / kg of methanolic extract of Terfezia boudieri (TBME) (0.2 ml) for 21 days. The blood samples were collected to determine the concentration of testosterone and finally, the weight and size of the testicles and epididymis, the number and the percentage of sperm moving were evaluated Data were analyzed using ANOVA and Turkey's post- hoc tests.
Results: The results of the present study showed that serum levels of testosterone, body weight of rat, testis weight , sperm count and sperm motility in the experimental group were significantly increased compared with the control group (p<0.001).
Conclusion: The results of this study showed that TB at 105 mg/kg dosage can increase the levels of testosterone and improve sperm parameters and therefore, TB can be used to treat sexual impotence and infertility in males.
Mina Kiani, Paria Parto ,
Volume 17, Issue 4 (1-2017)
Abstract
Background & objectives: The present study examined the gonadotoxic effects of ciprofloxacin antibiotics and protective effects of L-carnitine.
Methods: In this study, 20 NMRI mice were divided into four groups: control, ciprofloxacin, L-carnitine, L-carnitine-ciprofloxacin (each group included 5 animals). The control group received normal saline, the treatment group 1 received 12.5 mg/kg ciprofloxacin, the treatment group 2 received 100 mg/kg L- carnitine and the treatment group 3 received 100 mg/kg L- carnitine and 12.5 mg / kg ciprofloxacin simultaneously. All animals were treated by intraperitoneal administration for 15 days. Testis and epididymis were collected to evaluate sperm parameters (sperm count, motility, morphology and viability).
Results: In the ciprofloxacin group, a significant decrease in sperm count, sperm viability, sperm motility, progressive sperm motility and normal sperm as well as a significant increase in rotating sperm motility, sperm without movement, head and tail abnormalities were observed (p≤0.05). A significant increase in sperm count, sperm survival, motility and progressive sperm motility, normal sperm and a significant decrease in rotating sperm motility, sperm without movement and head abnormalities in the L-carnitine-ciprofloxacin group were observed (p≤0.05); however, the reduction in tail abnormalities of sperm was not significant.
Conclusion: L-carnitine improves sperm parameters in the adult mice treated with ciprofloxacin.
Shima Shafiee, Mino Mahmoodi, Siamak Shahidi ,
Volume 17, Issue 4 (1-2017)
Abstract
Background & objectives: exposure to lead has a wide range side effects on fertility. Vitamin D is one of the most important vitamins required for the body. This study was conducted to determine the effect of vitamin D on spermatogenesis in male rats treated with lead nitrate.
Methods: In this experimental study, 25 adult male Wistar rats (250-300 gr) were randomly divided into 5 groups (n=5). Control group without any treatment, the group receiving lead by gavage and experimental groups receiving lead plus vitamin D at doses of 25, 50 and 75 mg/kg body weight by gavage for 28 days. At the end of the study, after anesthetizing the rats, blood samples were collected directly from heart and serum levels of testosterone hormone, Follicle-stimulating Hormone (FSH) andLuteinizing Hormone (LH) were measured. Histological studies were performed to count the spermatocyte and examine the diameter of the seminal tube. Data were analyzed by SPSS software using one-way ANOVA, at significance level of p>0.05.
Results: Compared to the control group, the mean serum testosterone level in the lead group significantly decreased (p<0.001) and the mean LH and FSH serum levels significantly increased (p<0.001) . Also, spermatocytes and seminiferous tubule diameter significantly decreased (p<0.001). Vitamin D consumption reduced the effects of lead intake, and this effectiveness was completely dose-dependent.
Conclusion: Our data showed that vitamin D has a significant effect on serum testosterone levels and gonadotropins.
Taktam Sadat Vafa , Mojdeh Emadi , Seyed Damoon Sadoughi,
Volume 18, Issue 1 (4-2018)
Abstract
Background & objectives: Curcumin has antioxidant properties. The aim of this study was to determine the effect of curcumin on bax, bcl-2, antioxidant enzymes and lipid peroxidation of sperm after freezing procedure.
Methods: In this experimental study, semen samples were collected from four mature Holstein bulls, twice a week in eight innings. Semen samples were divided into four groups. Zero (control), 10 (Experimental group one), 20 (Experimental group two) and 30 (Experimental group three) mg/ml of curcumin with diluents were added to the semen samples. After thawing, Bax, Bcl-2 and malondialdehyde levels as well as superoxide dismutase, glutathione peroxidase and catalase enzymes were measured in sperm samples using ELISA.
Results: According to the results, Bcl-2, superoxide dismutase, glutathione peroxidase and catalase levels in sperm samples treated with 20 and 30 mg/ml curcumin significantly increased and Bax and malondialdehyde levels significantly decreased compared to control groups (p<0.05). This difference was not significant for sperm samples treated with 10 mg/ml curcumin.
Conclusion: Dose-dependent administration of curcumin decreased oxidative stress and lipid peroxidation and increased anti-apoptosis proteins in freeze-thawing sperms.
Ali Soleimanzadeh, Ali Shalizar Jalali, Abdulrahim Abdullahi, Mohammad Moein Sabzeie,
Volume 20, Issue 2 (7-2020)
Abstract
Background & objectives: The present study investigated the effects of royal jelly on lead acetate induced toxicity on sperm parameters, reproductive hormone assay, and bak gene expression in NMRI male mice.
Methods: In this study, fifty four male mice were randomly divided into nine groups: control group (without royal jelly) (n=6); sham group(10 ml normal saline) (n=6); lead group (1000 ppm, oral) (n=6); Group 4: royal jelly (100 mg/kg/day, oral) (n=6); Group 5: royal jelly (250 mg/kg/day, oral)(n=6); Group 6: royal jelly (500 mg/kg/day, oral)(n=6); Group 7: royal jelly (100 mg/kg/day, oral) + 1000 ppm lead (n=6); Group 8: royal jelly (250 mg/kg/day, oral) + 1000 ppm lead (n=6) and Group 9: royal jelly (500 mg/kg/day, oral) + 1000 ppm lead (n=6). On day 35, blood samples were collected from anaesthetized mice by cardiac puncture to assess reproductive hormones and the testes were harvested for determination of sperm parameters and expression bak gene. Sperm parameters including motility, viability, DNA damage, morphology and total antioxidant capacity (TAC) levels were determined.
Results: The results showed that administration of royal jelly significantly enhanced sperm parameters and all reproductive hormone levels compared to control mice, (p<0.05). Also, treatment with lead acetate caused a significant reduction in levels of all reproductive hormones and a significant diminution in sperm motility, morphology, viability; with an increase in percentage of dead spermatocytes (p<0.05). The co-administration of the 250 and 500 mg/kg/day royal jelly with lead acetate could ameliorate the deleterious effects of lead acetate resulting in a significant increase in sperm parameters and all reproductive hormones and increase the total antioxidant capacity (TAC) levels (p<0.05). Also, the expression of bak gene in all treated (sham, royal jelly groups) and control groups was significantly lower than the lead acetate group (p<0.05).
Conclusion: In conclusion, our findings suggest that the royal jelly has a beneficial effect on male reproductive parameters following lead acetate induced toxicity in mice.
Parisa Ghahremani, Hemen Moradi Sardareh, Hashem Yaghoubi, Neda Farazi, Asadullah Asady,
Volume 22, Issue 4 (1-2023)
Abstract
Background & objectives: Fenugreek (Trigonella foenum-graecum) is one of the most ancient and well-known medicinal plants, and its useful role has been well - established in treating of many diseases. The purpose of this study was to investigate the effect of Fenugreek on spermatogenesis, total antioxidant capacity and malondialdehyde (MDA) level in male diabetic rats.
Methods: In this experimental study, 24 male Wistar rats weighing approximately 200±20 gram were used. The rats were randomly divided into 4 groups of six. group1: control group, Group2: diabetic, Group3: diabetic+Fenugreek (8gram /kg diet), group4: diabetic+Fenugreek (2gram /kg diet). This treatment continued for 4 weeks. Blood glucose, total antioxidant capacity, malondialdehyde, and sperm profiles were determined.
Results: Blood glucose in diabetic rats which received Fenugreek significantly reduced compared with diabetic animals. Fenugreek also reduced body weight and MDA level of semen (p<0.05).On the other hand, Fenugreek increased the total antioxidant capacity of semen (p<0.05) and normalized sperm profiles (p<0.05).
Conclusion: According to the present results, it can be concluded that consumption of Fenugreek extract probably reduced MDA level, body weight, and normalized sperm profile thus, Fenugreek extract can be used to treat sexual dysfunction in males.
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