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Showing 3 results for Shv
Abbasali Imani Foolad , Zahra Rostami , Reza Shapouri,
Volume 10, Issue 3 (9-2010)
Abstract
Background and Objectives: Detection of TEM and SHV genes in ESBL producing Pseudomonas aeruginosa and their antimicrobial resistance pattern can provide useful information about the epidemiology and risk factors of associated infections. In this study we determined the antimicrobial susceptibility pattern and prevalence of ESBLs in clinical isolates of Pseudomonas aeruginosa by phenotypic and genotypic methods. Methods: In this analytic-descriptive study, 110 Pseudomonas aeruginosa strains isolated from different clinical specimens were used. The pattern of antimicrobial resistance was determined by disk diffusion (Kirby-buer) method. The ESBL production was determined by combination disk method using disks containing ceftazidim and cefotaxim alone and in combination with Clavulanic acid. SHV and TEM types of ESBL producing genes was detected by PCR. Results: In this study Co-trimoxazole and Amoxicilin with 96.4% and 92.7% and Amikacin with 17.3% showed the highest and lowest resistance against isolates respectively. According to PCR results 37.5% and 12.5% of isolate were carried SHV and TEM genes respectively and 12.5% of isolate were carried both the SHV and TEM genes. Conclusion: According to the results most of the isolates are drug resistant and among the ESBL producing strains the frequency of SHV type is higher than TEM . The isolate ceftazidim resistance was contains SHV (37.5%) and TEM gene (12.5%), that showed SHV and TEM genes play more important role in create of ceftazidim resistance than cefotaxim resistance.
S Farid, H Peeri Dogaheh , M Ghiami Rad ,
Volume 15, Issue 3 (10-2015)
Abstract
Background & objectives: Drug resistance is one of the most problems in controlling microbial infections which assumes to be ever-increasing problem all through the world. Production of extended-spectrum &beta-lactamases enzyme (ESBLS) can cause a resistance to antibiotics of gram negative bacteria. The main purpose of this study was to determine antibiotic sensitivity patterns and SHV-1 genes frequency in collected urinary samples from hospitalized patients in Ardebil.
Methods: 400 isolated Enterobacteriaceae from urinary samples were recognized using an ordinary biochemical method. Antibiotic sensitivity test was conducted by Kirby and Bauer. The combined disk method was also utilized as a confirmatory test. The results were compared to clinical and laboratory standards institute (CLSI) standards. Finally, ESBL positive isolates were investigated by PCR method regarding the SHV-1 gene.
Results: From the total of 400 urinary isolates Enterobacteriaseae resistance to Ampicilin, Nalidixicacid, Co-trimoxazole, Cefotaxime, Ceftazidime, Ceftriaxone, Ciprofloxacin Ceftizoxime, Cefixime, Gentamycin, Imipenem were 82.5, 62.3, 67, 36, 49.5, 50.3, 52, 36, 41, 24.8 and 7.7 percents, respectively. 150 isolates (37.5%) were positive ESBL, and among all, 28 isolates (18.7%) contained SHV-1 gene.
Conclusion: According to obtained results from the study, regarding high percentage of resistance to antibiotics and high reduction of ESBLS in bacteria from who suffered from urinary infections, taking some logical steps for prevention seems to be essential.
Hoosna Sarvazad, Mojtaba Darbouy,
Volume 17, Issue 3 (10-2017)
Abstract
Background & objectives: One of the main problems in the control of nosocomial infections due to Klebsiella pneumoniae is increase of antimicrobial resistance and prevalence of extended spectrum β-lactamase (ESBLs) producing isolates. The aim of this study was to investigate the correlation of antibiotics resistance with SHV, CTX-M and TEM extended-spectrum beta lactamases genes among Klebsiella pneumoniae isolates isolated from the patients in Kermanshah hospital.
Methods: The clinical isolates of Klebsiella pneumoniae were collected during the spring from Kermanshah hospitals, and identification of Klebsiella pneumoniae strains was performed using standard microbiological and biochemical tests. Antibiotic resistance of Klebsiella pneumoniae isolates was determined using disk diffusion method. Then, the presence of CTX-M, SHV, and TEM was investigated using multiplex-PCR method. Finally, the relationship between variables was analyzed by SPSS-22 software using logistic regression and chi-square.
Results: A total of 98 isolates out of 112 samples were identified as Klebsiella pneumonia. Also, 82.8% of isolates were resistant to cefotaxime, 40.2% to ceftriaxone, 62.88% to ceftazidime, 3.9% to imipenem, 39.17% to cefepime, 64.94% to cefixime and 26.8% to amikacin. Further, 35.55% of isolates had CTX-M gene, 63.91% of isolates had SHV gene and 9.27% of samples had TEM gene.
Conclusion: The presence of CTX-M, SHV and TEM genes along with high antibiotic resistance are very concerning, indicating the importance of rational use of antibiotic for the treatment of infectious diseases.
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