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Showing 15 results for Protein
Reza Ali Panah Moghadam , Mohamad Rahbani Nobar ,
Volume 6, Issue 2 (6-2006)
Abstract
Background & Objectives:Lipid peroxidation has recently attracted considerable attention. It has been linked to carcinogenesis, aging and variety of other diseases including atherosclerosis. Atherogenesis involves the complicated interaction between cells of the arterial wall and lipoproteins (mainly oxidized LDL). The aim of this study was to investigate the relationship between serum malodialdehyde (MDA), the important indicator of lipid peroxidation and alteration in serum lipids and lipoproteins concentrations in male patients with coronary artery disease (CAD). Methods: The subjects of this study were 51 male patients under 55 with angiographically comfirmed CAD admitted to Shahid Madani Hospital and 60 age and sex matched apparently healthy individuals as the control group. The serum level of MDA was measured by colorimetric method using thiobarbituric acid reaction and lipid and lipoproteins concentrations were determined by standard enzymatic methods. Results: Significant elevation in the level of MDA was noticed compared to the controls (P=0.03) . Serum concentrations of cholesterol, triglyceride, LDL-C and LDL - C /HDL - C ratio in cases were higher than those of the controls (P=0.03) . There was a positive and significant relationship between serum MDA and biochemical risk factors including Triglyceride, Cholesterol, LDL-C and LDL - C/HDL - C ratio (p=0.03). The correlation between serum MDA and serum HDL-C was negative and meaningful (P=0.03) . No relationship was observed between serum MDA with BMI and age in cases. Conclusion: Simultaneous control and monitoring of both dyslipoproteinemia and lipid peroxidation may be of equal importance in prevention of occurrence and progression of CAD.
Alireza Mohebbi Pour, Behrooz Shokuhi,
Volume 10, Issue 2 (6-2010)
Abstract
Lipoid proteinosis is a rare autosomal-recessive disturbance characterized by yellowish-white deposits on the inner surface of the lips, under surface of the tongue and uncommonly other surfaces of upper respiratory tract. Changes in larynx lead to degrees of hoarsness which usually appears within the first few weeks of life after initiation of the disease. The patient tongue is firm like as wood. In some patients deposits observed on the vocal cords, labia major, scrotum, gluteal folds and axilla. The eyelid margins contain yellowish pearly papules in about 2/3 of patients. Neurologic findings such as epilepsy are rare but pathognomonic. The gene of these disturbances is recognized on 1q21 choromosome. The patient reported here is a 13 years old girl with yellowish pearly papules on the margins of her eyelids and hoarsness. Diagnosis of lipoid proteinosis is confirmed by pathologic findings.
Mehrdad Mirzarahimi, Adel Ahadi , Afsaneh Enteshari Mogaddam, Manoochehr Barak,
Volume 11, Issue 3 (9-2011)
Abstract
Protein C is a vitamin K dependent glycoprotein. Protein C deficiency is a rare genetic disorder and its major sites of involvement are skin, eyes, lungs, central nervous system, and kidneys. This article presents a case of neonatal protein C deficiency with severe purpura fulminans and bilateral cataracts. He was initially treated with fresh-frozen plasma and then followed by warfarin. All necrotic skin lesions improved with treatment. He was the first child of his parents who were asymptomatic for protein C deficiency. We also reviewed literatures about coexistence of cataracts and protein C deficiency.
Sayeneh Khodadadi, Ashraf Mohabati Mobarez, Naser Harzandi , Bahman Tabaraei , Nima Khoramabadi, Amir Bakhtiyari , Hanyieh Aghababa ,
Volume 12, Issue 1 (4-2012)
Abstract
Background & Objectives: The identification of Brucella spp. antigens with the capacity to elicit a protective immune response is of the great interest for the researchers. So, characterization and assessment of diverse antigens of Brucella need to be evaluated. In this study, we report the cloning and expression of the gene coding for 31 KDa OMP (OMP31) of Brucella melitensis 16M. Methods: Brucella melitensis Omp31 gene was amplified with specific primers, cloned into pJET1/2 and subsequently subcloned in pET28a (+) vector. Both these recombinant plasmids were sequenced and then after, expression of recombinant protein was induced by 1mM IPTG. Western blot analysis was also performed by polyclonal rabbit antiserum. Results: Omp31 successfully was cloned in both plasmid vectors. The recombinant Omp31 was expressed in E.coli host and purified with significant yield. Western blot results along with those of sequencing ensured accurate production of recombinant omp31 and retaining of its partial epitopes. Conclusion: Our results show that, an expression host such as E. coli is suitable for omp31 production.
Golamhosein Ettehad, Neda Parastar, Yasamin Pahlavan, Mojtaba Amani,
Volume 12, Issue 3 (9-2012)
Abstract
Background & Objectives: Matrix Metalloproteinases (MMPs), a member of photolytic enzyme family, degrade the extra cellular matrix. MMPs have very important roles in physiological and pathological processes. It has been reported that MMPs concentration increase in malignancies such as stomach, breast, colon, lung, head and neck cancers . Infectionwith Helicobacter pylori is risk factor for gastric cancer and may increase the serum level of MMP-9. The aim of this study was to measure the concentration of MMP-9 in sera of patients infected with H. pylori. Methods: In a descriptive-experimental study, apparent healthy individuals who were refereed for stool and blood tests were randomly selected and their stools and sera samples were collected. A questionnaire containing age, sex, smoking and special diseases in family and type of their diseases was filled for every volunteer. The sera collected immediately after blood sampling and stored in -70oC until used. The concentration of MMP-9 was assessed using ELISA. Stool samples were used for detection of H. pylori antigen. Results: H. pylori positive, negative and equivocal volunteers were 52.38%, 29.76% and 17.85%, respectively. Differences between MMP-9 concentration and H. pylori negative and positive groups were not significant (p=0.25). A significant increase in MMP-9 concentration was found in sera from drivers in compare with housewives. Conclusion: Although there was an increase in serum concentration of MMP-9 in H. pylori infected person, differences between H. pylori negative and positive groups were not significant. The raised concentration of MMP-9 in drivers may arise from difference in their health condition in compare with the housewives. This study suggests that MMP-9 level in serum may increase before initiation of gastric cancer in H. pylori infected individuals.
Maryam Rafraf, Elaheh Mohammadi, Laya Farzadi, Mohammad Asghari-Jafarabadi, Siyamak Sabour,
Volume 12, Issue 4 (12-2012)
Abstract
Background & Objectives: Polycystic ovary syndrome (PCOS) is one of the major endocrine disorders among females. PCOS is associated with development of type 2 diabetes and cardiovascular disease. The objectives of this study were to determine the effects of omega-3 fatty acids on glycemic status and high sensitive C-reactive protein in women with PCOS. Methods: This double-blind randomized controlled clinical trial was conducted on 61 PCOS patients. Subjects in ω-3 fatty acids (n=30) and placebo (n=31) groups take 4 ω-3 fatty acids capsules (each one contained 180 mg eicosapentaenoic acid and 120 mg docosahexanoic acid) or placebo daily for 8 weeks. Anthropometric and biochemical measurments and food intakes were assessed at the beginning and at the end of the study. Results: ω-3 fatty acid supplementation caused significant decrease in serum levels of glucose, insulin (p < 0.001 for both) and insulin resistance (p = 0.002) at the end of the study in comparision with baseline values. This was also statistically significant compared to the placebo group (p < 0.001, p = 0.002 and p = 0.001, respectively). No statistically significant differences in serum levels of high sensitive C-reactive protein were obsereved in any of groups at the end of the study (p > 0.05). Conclusion: ω-3 fatty acid supplementation had some beneficial effects on glycemic status of PCOS patients and may be useful in prevention and control of metabolic conmplication of this syndrome.
Sodabeh Jahanbakhsh-Godehkahriz , Elnaz Naderi , Ashraf Mohamadkhani ,
Volume 13, Issue 3 (10-2013)
Abstract
Background & Objectives: Non-synonymous single nucleotide polymorphism (nsSNPs) which results in disruption of protein function are used as markers in linkage and association of human proteins that might be involved in diseases and cancers . Methods: To study the functional effect of nsSNP in cyclooxygenase-2 (COX2 ) amino acids, the nucleotide sequences encoding COX-2 gene in cancers were extracted from the NCBI (gi|223941909) data bank (283 cases) and analyzed by SIFT, I-Mutant 2.0, SNP and GO, PANTHER and FASTSNP servers. These servers involve programs that predict the effects of amino acid substitution on protein function, stability and missense . Results: COX-2 is an essential enzyme for the production of pro-inflammatory prostaglandins which are relevant to cancer development and progression. The substitutions in some positions such as R228H and S428A of COX-2 in most of cancers linked to reformed protein function through disruption in enzyme active site. Conclusion: Amino acid substitutions as a consequence of COX-2 nsSNPs have important role in human disease. Substitutions which are located in catalytic domain are important for the enzymatic function of COX-2 and associated with higher expression of COX-2.
Masoomeh Parvizi, Seyed Fazlollah Mousavi, Khadijeh Mohammadi , Mohsen Arzanlou,
Volume 16, Issue 3 (10-2016)
Abstract
Background & objectives: Streptococcus pneumoniae is one of the major causes of vaccine - preventable diseases worldwide. Current pneumococcal vaccines consist of serotype specific capsular polysaccharide antigen and do not offer full clinical protection against pneumococcal diseases. Due to such limitations, a new generation of protein-based pneumococcal vaccines is being developed. The objective of this study was to determine the distribution of gens encoding five protein antigens including pneumococcal histidine triad D and E (phtD, phtE), rlr- regulated gene A (rrgA), Autolysin (lytA) and Pneumococcal surface protein C (pcpC) among pneumococcal isolates collected from nasopharyngeal specimens in healthy children.
Methods: A total of 43 pneumococcal isolates were collected from nasopharyngeal specimens of healthy children attending the kindergartens in Ardabil province. The strains were identified using optochin susceptibility and bile solubility testes and further confirmed by amplification of capsular polysaccharide A gene (cpsA). PCR was used for screening the presence of pcpC, phtD, phtE, rrgA and lytA genes.
Results: 81.4 % of isolates were found to contain at least one of the tested genes. lytA, pcpC, phtE, phtD and rrgA were detected in 70, 60, 39.5, 35 and 25.5 percent of isolates, respectively. The results showed that the genes were not distributed consistently among the isolates and for obtaining a full coverage pneumococcal vaccine, multiple choices of these antigens should be included.
Mohammad Amani,
Volume 16, Issue 4 (1-2016)
Abstract
Alzheimer's disease (AD) is a common cause of dementia in elderly people that is accompanied by progressive cognitive decline and memory loss. The pathologic hallmarks of AD are synaptic and neuronal degeneration together with extracellular senile plaques containing amyloid-beta (Aβ) and the intracellular neurofibrillary tangles (NFTs) in the hippocampus and other cortical regions. Amyloid-beta peptide is believed to have a pivotal role in the pathogenesis of AD as a major component of the senile plaques. It acts as a trigger key of AD and is considered as the principal toxic factor in the pathogenesis of the disease. Accumulation of amyloid β protein (Aβ), a main component of the senile plaques, in the brain initiates a cascade of events that ultimately lead to neuronal dysfunction and cognitive deficits. Other proposed mechanisms for AD include impairment in cholinergic function, oxidative stress, inflammatory agents and glutamate-mediated excitotoxicity. AD is characterized neuropathologically by impaired cholinergic function, increased oxidative stress, neuroinflammation, neuronal cell death, synapses loss, cortical atrophy, deficiencies in steroid hormones and appearance of glutamate-mediated excitotoxicity.
Manoucher Iranparvar, Bahman Bashardust, Shadab Mirfakhrayi,
Volume 17, Issue 3 (10-2017)
Abstract
Background & objectives: Diabetes mellitus belongs to a group of common metabolic disorders characterized by hyperglycemia phenotypes. Diabetes mellitus causes secondary pathophysiological disorders in multiple organs of the body such as nephropathy, which causes many problems for patients and the health care system. In this study, the effect of pentoxifylline, a nonselective phosphodiesterase inhibitor, on reducing urinary protein excretion in diabetic patients was assessed.
Methods: In this clinical trial, 72 diabetic patients with proteinuria who were admitted to the endocrine and nephrology clinic were selected and divided into two groups. Checklists, including demographic data, etc. were completed. In group (A), Angiotensin-converting enzyme inhibitors (ACEI) or Angiotensin II receptor blockers (ARBs) were prescribed to reduce proteinuria, and in another group (B), in addition to ACEI or ARB drugs, pentoxifylline was prescribed. In the end, the results in both groups were compared in terms of further reduction of proteinuria.
Results: Most of the studied patients were male. There was a significant correlation between proteinuria (mean urinary protein excretion in 24 hours) and the effect of pentoxifylline on reducing proteinuria in patients with type II diabetes. Also, there was not a significant difference in systolic and diastolic blood pressure changes and HbA1c between the two groups at the beginning and end of the study.
Conclusion: Pentoxifylline, independent of lowering blood pressure or reducing the improvement of metabolic control, can significantly decrease proteinuria and protein excretion
Asghar Mahmoudi, Marefat Siauhkouhian , Manoucher Iranparvar, Hasan Anari, Farnaz Seifi,
Volume 17, Issue 4 (1-2017)
Abstract
Background & objectives: Non-alcoholic fatty liver disease (NAFLD) is the most common chronic liver disease in the world which is associated with cardiovascular disease. The aim of this study was to investigate the plasma changes of chemerin and pentraxin-3 (PTX3) following 8 weeks of endurance training in men with NAFLD.
Methods: In this quasi-experimental Study, thirty four patients with NAFLD were selected purposefully. The participants were randomly divided into two groups, endurance training group (n=17) and control group (n=17). The endurance training program included eight weeks running on a treadmill with maximum heart rate (HRmax) intensity of 55-75% for 45 minutes three times a week. Seventy two hours before the beginning of the study and at the end of the eighth week, blood samples were taken from all the participants. The data were analyzed by paired sample t-test and independent t-test at the significant level p<0.05.
Results: Plasma levels of chemerin in the endurance training group significantly decreased (p=0.013). Also, there was a significant difference between the two groups after the end of the intervention (p=0.002). Plasma levels of PTX3 increased significantly in the endurance training group (p=0.001). There was also a significant difference between two groups after the end of the intervention (p=0.01).
Conclusion: The present study showed that the 8-week endurance training increased the plasma levels of PTX3 and decreased the plasma levels of chemerin in men with NAFLD. Endurance training, possibly through various mechanisms, reduces the inflammatory factors and increases the anti-inflammatory factors in men with NAFLD. Endurance training, probably by activation of lipolysis, over-regulation of unpaired protein-1 and activator receptors of peroxisomes proliferation-, changes in adipocytokines, etc. decreases charmicin and increases PTX3 levels of plasma in men with NAFLD.
Rasoul Sharifi , Akbar Nazari ,
Volume 18, Issue 3 (10-2018)
Abstract
Background & objectives: Metalloproteinases (MMPs) are one of the most important agents in cartilage damage in rheumatoid arthritis. Increase of these enzymes can change sensitivity to insulin. Therefore, in this study, the MMPs level, insulin sensitivity index and effective factor related to rheumatoid arthritis were evaluated in obese female with rheumatoid arthritis.
Methods: In the present study, 50 obese women with rheumatoid arthritis who were referred to the Sheikh Al-Reis clinic and Sina hospital in Tabriz and 50 healthy obese individuals were considered as case group and control group respectively. After determining the body weight, the levels of MMP-1, MMP-3, MMP-13, insulin, leptin, visfatin, resistin and glucose were measured using ELISA and Pars -Azmoon kits. One-way ANOVA method was used for statistical analysis of data. Pearson correlation test was used to evaluate the correlation between variables (p<0.05).
Results: in the present study, the mean level of MMP-1 (p=0.008), MMP-3 (p=0.015) and MMP-13 (p=0.001) in the case group was significantly higher than the control group. Also, Insulin- sensitivity index analysis didn’t show significant difference between the case group and the control group (p=0.21). In the case group, the level of leptin (p=0.000), resistin (p=0.000) and visfatin (p=0.024) was significantly increased compared to control group while insulin (p=0.231) and glucose (p=0.430) levels didn’t show significant increase.
Correlation results showed that there was a negative correlation between insulin and MMPs and a positive correlation with other parameters affecting insulin sensitivity.
Conclusion: our results showed, the matrix metalloproteinase level and the level of some factors affecting insulin sensitivity have increased in rheumatoid arthritis disease. Also, there was a significant positive correlation between the matrix metalloproteinase and some parameters affecting insulin sensitivity, including resistin and visfatin.
Lale Naderi , Allahyar Arabmomeni,
Volume 19, Issue 3 (10-2019)
Abstract
Background & objectives: Due to the prevalence of obesity and the subsequent development of metabolic risk factors, cardiovascular and fatty liver complications, exercise programs and the use of natural supplements can play a significant role in controlling and preventing these diseases. Therefore, the purpose of this study was to review the effect of eight weeks of combined exercise and Chlorogenic acid intake on C-reactive protein and liver enzymes in obese women.
Methods: In this quasi-experimental study, which was performed as a pre-test, post-test with a control group, a total of 48 obese women were selected purposefully and divided randomly into four equal groups (n=12); exercise, supplement, exercise+supplementation and control group. The exercises program consisted of 8 weeks of aerobic and resistance training, 3 sessions with 60 minutes per week. The chlorogenic acid supplement group received daily Green coffee in capsule form contain 400 mg powder for 8 weeks and the concurrent group performed exercise and received Green coffee simultaneously. By using blood sampling the variables were measured at baseline and after 8 weeks of intervention. Data were analyzed by t-test and ANOVA at significance level of p<0.05.
Results: Alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in the supplement group were the lowest and in the exercise+supplementation group had the highest decrease and there was no significant difference in the control group. The C-reactive protein (CRP) in the training+supplementation group was significantly decreased and in the three experimental groups there was a significant difference compared to the control group (p≥0.05).
Conclusion: It seems that combined exercise and natural substances rich in chlorogenic acid can decrease inflammatory factors such as C-reactive protein and liver enzymes.
Mahdi Babaie,
Volume 20, Issue 2 (7-2020)
Abstract
Before describing the structure and mechanism of action of a protein, it must first be subject to purification procedure. Protein purification is a set of processes in which one or a small number of proteins are purified from a complex compound that may be a complete cell, tissue, or organism. Understanding the functions, structural properties, and interactions of the protein are directly related to the degree of purity of the protein of interest. In the purification process, the protein and non-protein parts are separated. The biggest challenge is when the protein must be separated from other proteins. The purification procedure of an unknown protein is usually depends on the size, physicochemical properties, binding affinity, and biological activity. The end product of the purification process is called protein isolate. The protein purification process usually involves filtration and one or more chromatographic steps. Chromatography is a useful method for acquiring very pure protein for using in very accurate experiments. Therefore, by purifying the desired molecule, it can be used in various industries, such as medicine.
Aida Nahumi, Maryam Peymani, Hussein A Ghanimi, Asadollah Asadi, Arash Abdolmaleki,
Volume 22, Issue 2 (7-2022)
Abstract
Background & objectives: One of the functions of RNA editing is to change the RNA sequence without changing the genomic DNA sequence and changing the fate of cellular RNA. Therefore, studying the clinical application of RNA editing for targeted therapies is necessary.
Methods: All articles related to the subject of the study were searched in the Scopus,
PubMed/Medline, ISI Web of Knowledge, and Google Scholar database.
Results: The changes that occur within the RNA editing are A to I base replacement by adenosine deaminase (ADAR) on RNA and C to U replacement by the apolipoprotein B mRNA-editing enzyme, catalytic polypeptide1 (APOBEC1). Recently, the role of RNA editing in human diseases has been reported.
Conclusion: RNA editing can be used as a new strategy to identify new disease biomarkers and more personalized treatments for various diseases.
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