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Showing 3 results for Mouse
Mohammad Ghasem Golmohammadi , Mohsen Sagha , Hasan Azari , Norouz Najafzadeh,
Volume 11, Issue 3 (9-2011)
Abstract
Background &Objectives: Stem cells are a class of undifferentiated cells that are able to differentiate into specialized cell types. The discovery of such cells in the adult mammalian central nervous system (CNS), an organ traditionally thought to have little or no regenerative capacity, opened the door to treatment of degenerative diseases of CNS like Stroke, Parkinson, Alzheimer and Spinal Cord Injury. Thus, here we described the isolation of neural stem cells from the adult mouse brain using the neurosphere assay (NSA) and differentiation of these cells to neural adult cells in details. Methods: The rostral part of the subventricular zone (SVZ) of the lateral ventricles in the adult mice was dissociated into single cell suspension and cultured using NSA. Primary neurospheres were counted seven days after plating and then the mean number of neurospheres was recorded. The differentiation of neural stem cells into adult neural cells was accomplished by plating the neurosphere-derived cells in differentiating media. Immunocytochemistry and specific markers were used for the identification of the adult neural stem cells. Results : The cell suspension obtained from the rostral part of the SVZ of the lateral ventricles generated multipotential colonies, called neurospheres, 7 to 10 days post- incubation. The mean number of neurospheres generated from SVZ was 505±62. The multipotentiality of the neurospheres was shown by palting them in differentiating media and generating adult neural cells including neuron, astrocyte and oligodendrocyte . Conclusion: Owing to their rarity and paucity of neural stem cell specific markers, the NSA is a common and selective method for isolating and understanding the biology of embryonic and adult neural stem cells.
Maryam Ghasemi , Farzad Rajaei, Darioush Mohammadnejad, Amir Javadi,
Volume 11, Issue 4 (12-2011)
Abstract
Background & Objectives: Stress in developing countries is an important problem in human health. Feelings of stress in humans result from interactions between persons and their environment. Stressor is an external stimulus or an event that provokes a stress response in an organism. Animal models enable preclinical testing of new treatments and therapies for physical symptoms of stress disorder. In the present study the effects of social stress on male mouse reproductive system were investigated. Methods: Sixty male mice were divided into 6 groups, including two non-stressed control groups (2 cages, 5 mice per cage), two mild-stressed groups (2 cages, 10 mice per cage), and two high-stressed groups (2 cages, 15 mice per cage). Three cages (one cage from each group) kept for one month and three cages kept for two months. After one and two months the mice were anaesthetized with ketamine and xylazine. Tissue samples of testes , epididymis and vas deferens for light microscopy were removed . Data analysis was performed using one-way ANOVA. P<0.05 was considered significant. Results: The results showed that there was no significant difference between mild and high-stresses groups in the diameter of seminiferous tubules, vas deferens, height of epithelial cells of epididymis and vas deferens. The diameter of epididymis in mild and high-stressed groups was significantly decreased as compared with that in non-stressed control groups (P<0.02, P<0.009). The diameter of vas deferens in mild-stressed groups was significantly decreased as compared with in non-stressed control groups (P<0.02). The height of epithelial cells of vas deferens in mild and high-stressed groups was significantly decreased as compared with that in non-stressed control groups (P<0.001, P<0.001). Conclusion: This study shows that crowding stress can decrease the diameter and height of epithelial cells of epididymis and vas deferens of male mice .
Mahdi Saadati , Mahdokht Taheri , Mohammad Hadi Bahadori ,
Volume 14, Issue 4 (1-2014)
Abstract
Background & objectives : Infertility is a global problem affecting millions of men and women in developed and developing countries. In this regard, in-vitro fertilization (IVF) plays an important role in improving the quality of life in infertile patients. However, studies have shown that the implantation failure in IVF is the main challenge of this procedure. Melatonin can increase the survival rate of embryos and IVF success rate through eliminating free radicals and removing reactive oxygen species. So, this study is conducted to investigate the effects of different concentrations of melatonin on the rate of newborns of mice following transfer oftwo-cell embryos . Methods : In this study, female mice with average age of six to eight weeks were superovulated by administering pregnant mares serum gonadotropin (PMSG) intraperitoneally (7.5 IU. ip), and followed after 48h by human chorionic gonadotropin (hCG) (7.5 IU. ip). Two-cell mouse embryos were obtained from female mice oviduct after 48 h. The embryos transferred bilaterally into pseudopregnant mice of the same strain through surgical procedure and 8-14 embryos were transferred to each tube. The study included 4 treatment groups and one control group (6 mice in each group). The treatment groups were exposed to subcutaneous injection of concentrations of 100 µm , 10 µm , 1 µm and 100 nm of melatonin. After the cesarean on 18th day of pregnancy, the percentage of live births was assessed. The outcomes of the live birth rate were assessed using the chi-square test and statistical analyses were carried out using SPSS version 16.0. Percentage of live birth was calculated and compared with the control group. Results: A total of 701 two-cell mouse embryos were transferred into one control group and four experimental groups. The number and percentage of live births at concentrations of 100 µm and 10 µm of melatonin and the control groups were 21 (15.55%), 13 (9.15%) and 9 (6.47%), respectively. No infant was born at the concentrations of 1 µM and 100 nM of melatonin . The highest rate of live births was obtained at the concentration of 100 µM and showed a significant difference with the control group (p ≤ 0.01). There was no significant difference in live births at the concentration of 10 µm and control group. Conclusion : The results of this study indicated that subcutaneous injection of melatonin improves the two-cell mouse embryo growth and post implantation development of mice.
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