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Showing 4 results for Mda
Reza Ali Panah Moghadam , Mohamad Rahbani Nobar ,
Volume 6, Issue 2 (6-2006)
Abstract
Background & Objectives:Lipid peroxidation has recently attracted considerable attention. It has been linked to carcinogenesis, aging and variety of other diseases including atherosclerosis. Atherogenesis involves the complicated interaction between cells of the arterial wall and lipoproteins (mainly oxidized LDL). The aim of this study was to investigate the relationship between serum malodialdehyde (MDA), the important indicator of lipid peroxidation and alteration in serum lipids and lipoproteins concentrations in male patients with coronary artery disease (CAD). Methods: The subjects of this study were 51 male patients under 55 with angiographically comfirmed CAD admitted to Shahid Madani Hospital and 60 age and sex matched apparently healthy individuals as the control group. The serum level of MDA was measured by colorimetric method using thiobarbituric acid reaction and lipid and lipoproteins concentrations were determined by standard enzymatic methods. Results: Significant elevation in the level of MDA was noticed compared to the controls (P=0.03) . Serum concentrations of cholesterol, triglyceride, LDL-C and LDL - C /HDL - C ratio in cases were higher than those of the controls (P=0.03) . There was a positive and significant relationship between serum MDA and biochemical risk factors including Triglyceride, Cholesterol, LDL-C and LDL - C/HDL - C ratio (p=0.03). The correlation between serum MDA and serum HDL-C was negative and meaningful (P=0.03) . No relationship was observed between serum MDA with BMI and age in cases. Conclusion: Simultaneous control and monitoring of both dyslipoproteinemia and lipid peroxidation may be of equal importance in prevention of occurrence and progression of CAD.
Tahereh Haghpanah, Vahid Sheibani, Mohammad Reza Afarinesh, Mousa Al Reza Hajzadeh, Khadijeh Esmail Pour Bezenjani, Yasamin Pahlavan,
Volume 12, Issue 4 (12-2012)
Abstract
Background & Objectives: I ntra-hippocampal adminestration of origanum (ORG) improves spatial learning of rats. T he aim of the present study was to investigate the possible mechanism for origanum extract on spatial learning and memory in the hippocampus. Methods : In this study 42 adult male Wistar rats were used . Animals were cannulated bilaterally in the posterior laterl of hippocampus. After the recovery period, the spatial learning and memory were assessed using Morris Water Maze (MWM). Saline, ORG (0.03μg/site) glutamate receptor antagonist MK801 (0.08, 0.2 and 0.4 μmol/site) and co-injected of MK + ORG was injected into the posterior lateral of hippocampus 20 minutes before the training and retrival sesions (for 5 consecutive days) (n=7). Results: The results showed that the intra-hippocampal injection of MK 801 significantly blocked the decreased distance and time of reaching (due to ORG injection) to find hidden platform of MWM (p<0.05). On the retrival tests, the average of time spent in the target area is reduced in the co-injected of MK801 + ORG group. Conclusion: The intra-hippocampal injection of aqueous extract oforiganum may improve working memory in rats through glutamate-dependent NMDA receptors.
A Mohammadi, B Baradaran,
Volume 15, Issue 3 (10-2015)
Abstract
Background & objectives: Cancer is one of the most causes of mortality in worldwide. Components derived from natural plants that induce apoptosis are used for cancer treatment. Therefore investigation of different herbal components for new anti-cancer drug is one of the main research activities throughout the world. According to low cost, oral consumption and easy access to the public extracts of Urtica dioica, in this study we aimed to investigate the effectiveness of this herb on MDA-MB-468 breast cancer cells.
Methods: Cytotoxic effect of Urtica dioica extract was measured using MTT assays. To show induction of apoptosis by this plant TUNEL and DNA Fragmentation test were performed.
Results: In the present study dichloromethane extracts noticeably killed cancer cells. IC50 values related to human breast adenocarcinoma cell line MDA-MB-468 were 29.46±1.05 µg/ml in 24 hours and 15.54±1.04 µg/ml in 48 hours. TUNEL test and DNA Fragmentation assay showed apoptotic characteristic in the extract treated cells.
Conclusion: The results showed that MDA-MB-468 cells after treatment with dichloromethane extract of Urtica dioica, induces apoptosis in MDA-MB-468 cancer cells which may be useful in the treatment of cancer.
Farid Manafi , Reza Alipanah-Moghadam , Ali Nemati, Vadood Malekzadeh, ,
Volume 17, Issue 4 (1-2017)
Abstract
Background & objectives: Andrographolide has potent antioxidant properties. There is still not a complete understanding of the antioxidant effects of andrographolide. In this study, the effect of andrographolide on the serum antioxidant activity of superoxide dismutase and catalase was investigated in the rats with secondary iron overload.
Methods: In this experimental study, 32 rats were divided into 4 groups of 8 each. The groups included: 1-Control 2- Receiving iron 3- Receiving iron treated with 7 mg/kg andrographolide 4- Receiving 7 mg/kg andrographolide. The serum activity of superoxide dismutase and catalase and serum level of malondialdehyde (MDA) was measured using colorimetric method. Prussian blue was used to evaluate iron deposition in liver.
Results: The results showed that iron was deposited in the liver. The activity of superoxide dismutase and catalase in the secondary iron receiving group, compared to control group, was significantly increased (p<0.05). The activity of catalase was significantly decreased in the group receiving iron and treated with andrographolide, compared to the control and iron receiving groups (p<0.05). Also, the activity of superoxide dismutase in the group receiving iron and treated with andrographolide decreased significantly compared to the iron receiving group (p<0.05). Serum levels of malondialdehyde in the iron receiving group were higher than the control group (p<0.05). Serum levels of malondialdehyde in the group receiving iron and treated with andrographolide, compared with the iron receiving group, showed a significant decrease (p<0.05).
Conclusion: Andrographolide (7mg/kg) reduced the serum activity of catalase and superoxide dismutase and malondialdehyde serum levels in rats with secondary iron overload.
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