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Showing 7 results for Follicle
Maliheh Nobakht , Norouz Najafzadeh, Bagher Pourheydar, Mohammad Ghasem Golmohammadi,
Volume 11, Issue 2 (6-2011)
Abstract
Background & objectives: During lifetime, hair follicles undergo three cyclic changes: anagen, catagen, telogen. In hair follicles, stem cells located in Bulge area, which is part of the outer root sheath. Bulge cells proliferate new cells in anagen phase. Bulge region of hair follicle indicated as a source of stem cell for many years, but little studies done in vitro to characterize rat bulge region hair follicle stem cells. Methods: In this study Bulge cells of rat hair follicle were isolated and cultured, then morphological features of these cells surveyed. Nestin and CD34 as hair follicle stem cell markers, and K15 as a keratinocyte marker assessed by immunocytochemistry after one to three weeks. Results: In this study, we found that, 2 days after attachment of cells to floor of plates, the cells were initiated to proliferation and migration. These cells had good nestin and CD34 immunostaining, but after three week differentiation,were nestin and CD34 negative. Also these cells couldn’t express K15. Conclusion : Results showed that cultivated rat bulge cells, have high proliferative potential, and also could express nestin and CD34 as stem cell factors.
Mohammadreza Behvarz , Masoud Maleki , Mohammadreza Mashayekhi ,
Volume 14, Issue 4 (1-2014)
Abstract
Background & objectives: Adult stem cells are undifferentiated cells that replace dead or injured cells. There are adult stem cells in some regions of human tissues and hair follicle is one of the tissues that have adult stem cell source and these cells have an important role in hair life cycle. In this study, we investigated the isolation of hair follicle stem cells (HFSCs) and expression of mesenchymal stem cell markers on the isolated cells. Methods : Human hair follicles obtained from men scalp tissue by micro punch technique. Hair follicles isolated and cultured in culture flasks in DMEM-F12 + FBS. After outgrowth of stem cells from hair bulges, they analyzed by flow cytometry for detection of stem cell markers. Results: 23 to 27 days after isolation and culture of HFSCs in uncoated cell culture flasks, cell surface markers expression studied by flow cytometry. Flow cytometric analysis showed 25.26% Stro-1, 50.85% CD90, 45.24% CD105, 61.20% CD44, 8.20% CD45, 11.86% CD146, 2.72% CD106, 7.21% CD166 and 26.74% CD19 expression in HFSCs. Conclusion: In this study, isolated stem cells significantly expressed some of the mesenchymal stem cell markers higher than other markers. These markers give certain characteristics to HFSCs, and introduce the cells as an alternative option for cell therapy, tissue engineering and regenerative medicine.
Sh Heydari Tajaddod, N Najafzadeh, M Mahdavi Rad, H Sheikhkanloui Milan, H Kalarestaghi, V Nejati,
Volume 15, Issue 2 (7-2015)
Abstract
Background & objectives: Hair follicle stem cells (HFSCs) are multipotent and various types of HFSCs were introduced. HFSCs separation using cell surface markers is one of the interesting strategies in the replacement of old methods. In this study, we used magnetic activating cell sorting (MACS) to separate HFSCs.
Methods: In this study, HFSCs were isolated from Balb/c mice and dissected under an invert microscopy, and bulge area isolated and the bulge cells cultivated about 14 days. The CD34 positive cells isolated using CD34 monoclonal antibody and magnetic activated cell sorting system (MACS), then the cells incubated in DMEM/F12 and 10% FBS. The CD34 positive cells counted using a neubauer slide and evaluated under a fluorescent microscopy.
Results: Here, we isolated CD34 positive cells using MACS and 12±1. 04% of HFSCs were CD34 positive and we found that, CD34 positive cells survived during 7 days cell culture in vitro.
Conclusion: The results show that MACS is useful to increasing density gradient of cells in vitro.
Ebrahim Hosseini, Zahra Zia,
Volume 16, Issue 1 (4-2016)
Abstract
Background & objectives: The teratogenic effects of electromagnetic radiation on different processes of growth caused many concerns related to the harmful effects of cell-phone radiation on human health. Therefore, the aim of this study was to investigate the effects of cell-phone radiation on estrogen, progesterone, FSH and LH hormones together with dynastic sexual cells of adult female offspring of pregnant rats affected by these radiations.
Methods: In this experimental study, 24 pregnant female rats divided into 3 groups including the control, sham and experimental groups were used. The control group received no radiation and the experimental group was exposed to cell-phone radiation at the beginning of pregnancy (4 hours daily for 14 days). The control group was exposed around turning-on cell-phone without conversation over the same period. After giving birth and after maturity, 10 female offsprings of different groups separated and after phlebotomizing, sexual hormones levels was measured and by separating the ovaries, ovarian follicles species were counted. The results analyzed using ANOVA and T tests. Differences in statistical analysis of data were considered significant at p<0.05.
Results: The results showed that the pregnant female exposure to cell-phone radiation caused significant increase in the size and weight of the ovaries and atresic follicles (p<0.05) without significant effect on the number of primary and secondary follicles, antral, graph, primordial, corpus luteum and sexual hormones.
Conclusion: Exposure to cell-phone radiations caused increase in the size, weight and atresic follicles of offspring’s ovaries in pregnant females
Zahra Mirzakhani, Sayed Ebrahim Hosseini,
Volume 17, Issue 1 (4-2017)
Abstract
Background & Objective: Nowadays, female infertility and abortion is considered one of the most important issues in the medical world. Due to high consumption of chamomile as a medicinal herb, this study aimed to investigate the effects of chamomile consumption on abortion, estrogen, progesterone, FSH, LH hormones and ovarian follicles in adult female rats.
Methods: In this experimental study, 80 adult female rats were divided to 2 categories in 5 groups of 8 pregnant and non-pregnant rats, including control groups, sham group and groups receiving intraperitoneal doses of 30, 60 and 120 mg/kg chamomile hydro-alcoholic extract. At the end of the day 16 of pregnancy, aborted fetuses in pregnant groups were counted, and in day 21, the number of follicles and corpora-lutea in non-pregnant groups was obtained by separating ovaries, and sexual hormone levels were measured after phlebotomizing the samples. The results were analyzed by SPSS software (Ver.18) using ANOVA and Tukey tests. Significant difference of data was set at p≤0.05.
Results: The results of this study showed that chamomile caused a significant increase in the number of aborted fetuses and follicle atresia and a significant decrease (p≤0.05) in serum level of estrogen, progesterone, FSH and LH hormones as well as the number of pre-antral follicle, antral follicles, graph and corpora-lutea.
Conclusion: The results showed chamomile extract decreased LH and FSH, thereby decreasing ovarian follicles, sexual hormones and aborted fetuses.
Seyeddamoon Sadoughi ,
Volume 17, Issue 2 (7-2017)
Abstract
Background & objectives: Polycystic ovarian syndrome (PCOS) is a complex endocrine and metabolic disorder, which is characterized by ovulatory dysfunction and hyperandrogenism. Crocin is the main component of saffron. According to antioxidant properties and protective effects of crocin on gonads, this study was done to evaluate the effect of crocin on serum levels of gonadotropin, β-estradiol, testosterone and ovarian follicle in a rat model of polycystic ovarian syndrome
Methods: In this experimental study, 28 Wistar rats were divided into 4 equal groups. Including: control, non-treated PCOS and two PCOS groups treated with crocin (50 and 100 mg/kg). Polycystic ovarian syndrome was induced by 28 days injection of 1 mg/kg letrozole. Crocin was intraperitoneally administered into treated PCOS groups for 28 days. Saline solution was injected to the animals of control and non-treated PCOS groups. At the end of period treatment, serum levels of LH, FSH, testosterone and β-estradiol was measured using ELISA. Then, ovarian tissue samples were stained with hematoxylin-eosin and histological changes were examined. Data were analyzed using one-way ANOVA and Post Hoc Tukey statistical tests (p<0.05).
Results: Serum level of LH, testosterone, β-estradiol and the number of cystic follicles in the PCOS group treated with 100 mg/kg crocin compared to the non-treated PCOS group significantly decreased and FSH, the number of preantral follicles, antral and corpus luteum significantly increased (p<0.05).
Conclusion: Crocin has been effective in improving ovarian cysts and hormonal disorders in rats with polycystic ovarian syndrome.
Reza Javanshir, Hossein Kalarestaghy, Ramin Salimnejad,
Volume 24, Issue 2 (7-2024)
Abstract
Background: Premature ovarian insufficiency (POI) is the cessation of ovarian function in women under 40. One of the mechanisms involved in the induction of POI is oxidative stress. This study aims to investigate the effect of caffeic acid on the morphometrical changes of ovarian follicles of POI model mice.
Methods: Thirty-two female mice were randomly divided into 4 groups (n=8): 1) Control group (cont), 2) Caffeic acid group (CAF), 3) Premature ovarian insufficiency group (POI), and 4) Premature ovarian insufficiency + Caffeic acid group (POI+CAF). POI was induced through daily subcutaneous injections of D-galactose (200 mg/kg) for 6 weeks. Caffeic acid (60 mg/kg, intraperitoneal) was injected daily for 4 weeks from 15th day. One day after the last injection, the mice were anesthetized and the ovaries were removed. Then, the morphometric changes of ovarian follicles were examined using hematoxylin-eosin (H&E) staining.
Results: The results showed that D-Galactose-induced POI significantly decreases the diameter of primary, secondary, and antral follicles (P<0.05). Administration of caffeic acid in the POI+CAF group significantly prevented the reduction of follicle diameter (P<0.05).
Conclusion: The results showed that caffeic acid can prevent the reduction of the diameter of ovarian follicles in POI model mice.
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