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Showing 2 results for D-Galactose
Reza Javanshir, Hossein Kalarestaghy, Ramin Salimnejad,
Volume 24, Issue 2 (7-2024)
Abstract
Background: Premature ovarian insufficiency (POI) is the cessation of ovarian function in women under 40. One of the mechanisms involved in the induction of POI is oxidative stress. This study aims to investigate the effect of caffeic acid on the morphometrical changes of ovarian follicles of POI model mice.
Methods: Thirty-two female mice were randomly divided into 4 groups (n=8): 1) Control group (cont), 2) Caffeic acid group (CAF), 3) Premature ovarian insufficiency group (POI), and 4) Premature ovarian insufficiency + Caffeic acid group (POI+CAF). POI was induced through daily subcutaneous injections of D-galactose (200 mg/kg) for 6 weeks. Caffeic acid (60 mg/kg, intraperitoneal) was injected daily for 4 weeks from 15th day. One day after the last injection, the mice were anesthetized and the ovaries were removed. Then, the morphometric changes of ovarian follicles were examined using hematoxylin-eosin (H&E) staining.
Results: The results showed that D-Galactose-induced POI significantly decreases the diameter of primary, secondary, and antral follicles (P<0.05). Administration of caffeic acid in the POI+CAF group significantly prevented the reduction of follicle diameter (P<0.05).
Conclusion: The results showed that caffeic acid can prevent the reduction of the diameter of ovarian follicles in POI model mice.
Hossein Kalarestaghi, Mir-Mahdi Hosseini, Ramin Salimnejad,
Volume 24, Issue 3 (10-2024)
Abstract
Background: Liver aging is an important risk factor for chronic liver diseases. Oxidative stress is considered a common pathological mechanism for liver aging. This study aims to investigate caffeic acid's effects against liver injuries in a D-galactose-induced mouse aging model.
Methods: Forty male mice were randomly divided into 5 groups (n=8): 1) control (Con); 2) Sham; 3) caffeic acid (CA), 4) aging (Ag), and 5) aging+ caffeic acid (Ag+CA). The aging model was induced through daily intraperitoneal (i.p) injections of D-galactose (300 mg/kg) for 6 weeks. Caffeic acid (60 mg/kg, i.p.) was injected daily for 6 weeks. One day after the last injection, the mice were anesthetized, blood was withdrawn (for liver enzymes evaluation) and the liver was removed. The histopathological changes in the liver were examined using hematoxylin-eosin staining.
Results: The results showed that D-galactose-induced aging significantly increases the level of liver enzymes (AST, ALT and ALP) as well as liver tissue destruction compared to the control and sham groups (p<0.05). Treatment with caffeic acid in the Ag+CA group significantly decreased the level of liver enzymes and tissue damage index (p<0.05).
Conclusion: The results indicated that caffeic acid can reduce the destructive effects of D-galactose-induced aging in liver tissue.
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