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Showing 4 results for Carbon Tetrachloride
Yavar Mahmoodzadeh , Mohammad Mazani, Lotfollah Rezagholizadeh, Aliasghar Abbaspour, Eslam Zabihi, Pirooz Pourmohammad, Volume 16, Issue 4 (1-2016)
Abstract
Background & objectives: Some herbs contain compounds with antioxidant activity and can be used to protect or cure damages caused by chemical toxins. The aim of this study was to evaluate the effects of carbon tetrachloride (CCl4) on total antioxidants in various tissues and Tanacetum parthenium impacts on reducing devastating effects of carbon tetrachloride.
Methods: A total of 42 male Wistar rats were divided into seven groups of six animals in each group: normal control, damaged control, three groups that treated with 40, 80, and 120 mg/kg of Tanacetum parthenium extract 14 days before CCl4 injection and two groups served as post-treatment groups that received 80 and 120 mg/kg extract 2, 6, 24, and 48 h after CCl4 injection. At the end of study the liver, kidney, testis, and heart were removed and then homogenized and then the antioxidant activity of the tissues assessed using FRAP method. Results were analyzed by one-way ANOVA test.
Results: The results showed that the injection of carbon tetrachloride significantly decreasestotal antioxidant in both liver (p<0.001) and kidney (p<0.05) tissues. Administration of extract significantly (p<0.05) increased the total antioxidant of liver and kidney.
Conclusion: Protective effect of Feverfew against CCl4 induced damages is more effective in liver and kidney than testis and heart..
Yavar Mahmoudzade, Seyedeghbal Motavalibashi, Khaiam Bamdad, Eslam Zabihi, Hamid Sheikhkanloui Milan , Nasrin Hamidi, Volume 17, Issue 1 (4-2017)
Abstract
Background & objectives: Terfezia boudieri (TB) has more than 15 types of proteins, gallic acid, catechins, flavonoids, tannins and other compounds such as beta-carotene and linoleic acid. Some of these compounds have antioxidant and anti-hyperlipidemia effects. Therefore, this study aimed to examine the antioxidant and protective effects of this fungus on the liver injury induced by carbon tetrachloride.
Methods: 30 male Wistar rats were divided randomly into 5 groups (each group containing 6). Group 1 (normal control group) and group 2 (control CCl4) were given normal saline for 14 days. Group 3, 4 and 5 received 500, 1000 and 1500 mg/ kg doses of methanol extract of TB for 14 days, respectively before the injection of carbon tetrachloride. In the fourteenth day, all groups except group 1 were given 1.5 ml/kg of carbon tetrachloride (V / V 1: 1 dissolved in olive oil). Group 1, instead of carbon tetrachloride, received 1.5 ml/kg of olive oil on the fourteenth day.
Results: Injection of carbon tetrachloride in rats increased serum activity of ALT, AST, ALP and serum degrees of triglycerides, VLDL, LDL, HDL, total cholesterol, glucose, and reduced HDL and total antioxidant capacity (TAC); while pre-treatment with methanol extract of TB significantly reversed the changes.
Conclusion: The results of this study showed that methanol extract of TB can protect the liver against oxidants and free radicals produced by carbon tetrachloride metabolism.
Masoud Ojarudi, Mohammad Mazani, Reza Hajihosseini, Alireza Moradi, Lotfollah Rezagholizadeh, Volume 18, Issue 4 (1-2018)
Abstract
Background & objectives: Considering the importance of liver toxicity due to drugs and toxins as a major cause of liver damage, this study was conducted to evaluate the effects of the combination of cinnamon and ginger extracts on serum biochemical parameters in carbon tetrachloride (CCl4)- intoxicated rats.
Methods: In this experimental study, forty-two male Wistar rats were divided into 7 groups (n=6). Three control groups including normal control, combined extract control and damaged control were designated that received distilled water, a mixture of cinnamon and ginger extracts and distilled water respectively for 14 days.
Other 4 groups were pre-treatment groups which were treated with cinnamon (50 mg/kg/d), ginger (250 mg/kg/d), cinnamon + ginger (25+125 mg/kg/d) and silymarin (100 mg/kg/d) respectively for 14 days before being damaged by intraperitoneal injection of CCl4 (1 ml/kg, 1:1 in olive oil). Fifty - two hours after CCl4 injection, blood sampling was performed and biochemical parameters were measured in serum of rats.
Results: The results of this study showed that the injection of CCl4 significantly increased the ALT and AST enzymes activity ,cholesterol, triglyceride, and LDL levels and decreased the blood albumin and HDL levels in damaged rats compared to the normal control group (p<0.001). However, pre-treatment with the cinnamon and ginger extracts, especially combination of extracts significantly improved these values compared to the CCl4 damage group (p<0.001).
Conclusion: The results suggest that simultaneous use of cinnamon and ginger extracts has more significant protective effects against CCl4-induced hepatotoxicity.
Hamed Fouladi, Lotfollah Rezagholizadeh, Masoud Ojarudi, Amir Khalafi, Mohammad Mazani, Sina • mahdavifard, Volume 20, Issue 3 (10-2020)
Abstract
Background & objectives: The liver is one of the most important organs in the body and plays an essential role in the metabolism of drugs and toxins. Therefore, any damage to the liver can disrupt its function and cause various diseases. On the other hand, due to the increasing use of the medicinal herbs, in this study, the effects of hepatic protection of aqueous extracts of Humulus lupulus on liver damage induced by carbon tetrachloride in rats were investigated.
Methods: In this experimental study, 36 male Wistar rats were divided into 6 groups (n=6). Group 1 (normal control) and Group 2 (control CCl4): administered with distilled water orally for 14 days. Groups 3, 4, and 5 received 50, 100, and 200 mg/kg of Humulus lupulus extract for 14 days respectively. Group 6: administered with silymarin (as standard drug) 100 mg/kg orally for 14 days. All of the groups except group 1 received 1 ml CCl4 (1:1 v/v dissolved in olive oil) on 14th day. All animals were sacrificed and blood samples were collected to determine the biochemical parameters.
Results: Pretreatment with a Humulus lupulus extract significantly reduced the serum levels of ALT, AST, ALP, TG, and increased the serum HDL levels, but did not have a significant effect on GGT and LDL levels.
Conclusion: The results of this study showed that the Humulus lupulus extract can protect the liver from damage caused by carbon tetrachloride and have beneficial effects on lipid profile.
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