|
|
|
 |
Search published articles |
 |
|
Showing 3 results for Artemisia
Shokouh Chegini, Mina Ramezani, Solmaz Shahla,
Volume 18, Issue 2 (7-2018)
Abstract
Background & objectives: Artemisia absinthium (known as wormwood) is used as an antihelminthical, antimalarial, antiseptic and anti-inflammatory agent, and is used for treatment of gastric pain in the traditional medicine. In the present study, we evaluated the effects of hydroalcoholic extract of A. absinthium on the ovary tissues and pituitary_gonadal axis of the adult female NMRI mice.
Methods: In this experimental study, intraperitoneally (IP) injections of hydroalcoholic extract of A. absinthium, were performed over 30 days on 50 mice with 50 (first group), 100 (second group), and 150 mg/kg.bw (third group) doses. The sham group was received distilled water and control group received no injection. After 30 days of injections, the animals were dissected, and blood samples were collected by heart aspiration. The levels of LH, FSH, estrogen and progesterone of serum were measured by ELISA method. Seven µm sections of ovary were prepared by a microtome and stained by H&E method. Data were analyzed by One-Way ANOVA and Tukey post- hoc test. The Significance level was set at p<0.05.
Results: Our findings indicated a significant reduction (p<0.05) in the body weight in all experimental groups compared with sham and control groups. In parameters such as: large and small diameters of the ovary , number of primary, secondary, growing, graafian follicles, and corpus luteum, a significant decrease was observed in 100 and 150 mg/kg doses (p<0.05). In all experimental groups, no significant changes were observed in estradiol and progesterone levels. However, FSH and LH showed a significant decrease in 100 and 150 mg/kg doses (p<0.05).
Conclusion: Therefore, it can be concluded that Artemisia absinthium hydroalcohlic extract in high doses has damaging effects on the process of oogenesis, which may be due to α and β Thujoun, Alkaloid and Saponin constituents in this plant.
Ramin Abiri, Samira Ghasemi, Malihe Bagheri Ghomi, Masoumeh Shahbazi, Ahmad Emami, Mahdi Mojarrab,
Volume 23, Issue 3 (10-2023)
Abstract
Background: Nosocomial infections are significant parts of the treatment challenges of hospitals. Different species of the Artemisia genus are widely distributed in Iran. The present study aimed to compare the in vitro effects of different aqueous extracts of Artemisia species against nosocomial bacterial strains.
Methods: 24 different aqueous extracts, including decoctions and infusions were prepared from the aerial parts of Artemisia ciniformis, A. turanica, A. kopetdaghensis, A. khorasanica, A. vulgaris, and A. sieberi. Total phenolic contents were determined by the Folin-Ciocalteu method. The minimum inhibitory concentration (MIC) values of the extracts against the bacterial strains including Staphylococcus aureus, Streptococcus pyogenes, Acinetobacter sp., Pseudomonas aeruginosa, Enterococcus faecalis, Staphyloccocus epidermidis, Micrococcus luteus, Klebsiella neumonia and Escherichia coli were measured using the microdilution broth method.
Results: The lowest MIC value was observed for the decoction of A. turanica (0.25 mg/ml) against S. pyogenes. The largest range of bacterial sensitivity (3 strains) was related to the decoction of A. sieberi. The growth of S. epidermidis was inhibited by a larger group of extracts. The highest total phenolic content was recorded for decoction and infusion of A. turanica.
Conclusion: The decoctions of the aerial parts of A. sieberi and A. turanica were superior to the other extracts in terms of the in vitro antibacterial spectrum and selective potency, respectively.
Ramin Abiri, Samira Ghasemi, Nastaran Sharei, Masumeh Shahbazi, Seyed Ahmad Emami, Mahdi Mojarrab,
Volume 24, Issue 1 (4-2024)
Abstract
Background: Nosocomial infections are considered as the important parts of the treatment challenges in hospitals. The genus Artemisia is widely distributed in Iran. Their species produce antibacterial, antiviral, and antifungal compounds belonging to different groups including phenols, terpenoids, sterols and polyacetylenes. The purpose of the present study is to investigate the in vitro effects of different hydroethanolic extracts of Artemisia species against bacterial strains in nosocomial infection.
Methods: 12 different extracts, including 50% and 70% hydroethanolic extracts were prepared from the aerial parts of Artemisia ciniformis, A. turanica, A. kopetdaghensis, A. khorasanica, A. vulgaris, and A. sieberi. The winterization of the extracts produced corresponding defatted extracts. The minimum inhibitory concentration (MIC) values of the extracts against the bacterial strains Staphylococcus aureus, Streptococcus pyogenes, Acinetobacter sp., Pseudomonas aeruginosa, Enterococcus faecalis, Staphylococus epidermidis, Micrococcus luteus, Klebsiella pneumoniae and Escherichia coli were measured using the microdilution broth method. Total phenolic contents were determined by the Folin-Ciocalteu method.
Results: Among the extracts analyzed in this experiment, the lowest MIC value was observed for the 50% hydroethanolic extract of A. turanica (0.25 mg/ml) against S. epidermidis. The largest range of bacterial sensitivity (6 strains) was related to the 50% hydroethanolic extracts of A. turanica (defatted and non-defatted) and A. kopetdaghensis (defatted). The growth of S. epidermidis was inhibited by all of the extracts. The highest total phenolic content and yield of extraction were recorded for 70% hydroethanolic extract of A. sieberi and 50% hydroethanolic extract of A. ciniformis, respectively.
Conclusion: The 50% hydroethanolic extract of A. turanica was superior to the other extracts in terms of the in vitro antibacterial spectrum and selective potency. A. turanica and A. kopetdaghensis are probably suitable choices for further phytochemical and antibacterial investigations.
|
|
.png)
.png)
