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Showing 4 results for خدادادی
Sayeneh Khodadadi, Ashraf Mohabati Mobarez, Naser Harzandi , Bahman Tabaraei , Nima Khoramabadi, Amir Bakhtiyari , Hanyieh Aghababa ,
Volume 12, Issue 1 (spring 2012)
Abstract
Background & Objectives: The identification of Brucella spp. antigens with the capacity to elicit a protective immune response is of the great interest for the researchers. So, characterization and assessment of diverse antigens of Brucella need to be evaluated. In this study, we report the cloning and expression of the gene coding for 31 KDa OMP (OMP31) of Brucella melitensis 16M. Methods: Brucella melitensis Omp31 gene was amplified with specific primers, cloned into pJET1/2 and subsequently subcloned in pET28a (+) vector. Both these recombinant plasmids were sequenced and then after, expression of recombinant protein was induced by 1mM IPTG. Western blot analysis was also performed by polyclonal rabbit antiserum. Results: Omp31 successfully was cloned in both plasmid vectors. The recombinant Omp31 was expressed in E.coli host and purified with significant yield. Western blot results along with those of sequencing ensured accurate production of recombinant omp31 and retaining of its partial epitopes. Conclusion: Our results show that, an expression host such as E. coli is suitable for omp31 production.
Mohadeseh Rezaei Farahabadi, Javad Khodadadi Sangdeh, Sepide Imanian Najafabadi,
Volume 21, Issue 1 (spring 2021)
Abstract
Background & objective: Despite of significant advances in medicine, infectious diseases still threaten the health of society. A new type of Coronavirus called Covid-19 has become increasingly common in the world. Because of quarantine, the interactive patterns between family members, especially couples with Corona was affected and changed. The main goal of this research is to study the factors affecting interactive patterns between couples in the Corona period according to the conditions of the disease and home quarantine.
Methods: This study is a qualitative research and was done by Phenomenological method. The Participants included 15 men and women coronavirus survivors in 2020 that were selected by targeted sampling method. Semi-structured interviews were used to collect data.
Results: This research findings indicated that 5 main themes included: Changing roles, experiencing negative emotions, Coping behaviors, Support and Communication problems and 22 sub-themes.
Conclusions: The results showed that the performance of couples can play an effective role in the transition from crisis and disease and the type of their behaviors in this period can affect marital satisfaction or dissatisfaction.
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