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Showing 884 results for Type of Study: article
Masoomeh Saboorifar, Ali Shamsazar, Asadollah Asadi, Mostafa Shourian,
Volume 23, Issue 3 (10-2023)
Abstract
Background: Determining the concentration of hydrogen peroxide in liquids and biological samples is very important because of its effects on human health. This study aimed to design a new electrochemical biosensor based on hemoglobin to detect hydrogen peroxide in serum samples.
Methods: In this study, a basic science, a biosensor based on modifying the glassy carbon electrode surface with a nanocomposite consisting of cobalt oxide nanoparticles and multi-walled carbon nanotube functionalized with a carboxyl group (MWCNT/Co3O4) and hemoglobin stabilized on this nanocomposite was made as a biological recognition element.
Results: In optimal conditions, the biosensor was used to measure different concentrations of hydrogen peroxide. The designed biosensor showed a wide linear response range from 10 μM to 500 μM, a detection limit of 0.512 μM, and high reproducibility and stability.
Conclusion: In this innovative research work, MWCNTs/Co3O4 nanocomposite was used to make a diagnostic biosensor. The presented biosensor showed an acceptable performance in the measurement of hydrogen peroxide in serum samples and laboratory solutions.
Raziye Alizadeh, Amirali Jafarnezhadgero, Davood Khezri,
Volume 23, Issue 3 (10-2023)
Abstract
Background: Anterior cruciate ligament reconstruction (ACLR) reestablishes electromyography activity during moderate activities such as walking but it is unclear whether this is also the case in sports activities such as running. Therefore, the present study aimed to evaluate the acute effect of using anti- pronation insoles on muscle frequency content in an individual with anterior cruciate ligament reconstruction with pronated feet during running.
Methods: The sample of this study consisted of 13 individuals with both ACLR and pronated feet aged 23.2±4.5 years and 13 healthy ones aged 22.9±4.1 years. During both Pre and Post-tests, participants ran barefoot on an 18-m runway at a constant speed of 3.3±5 m/s. The electromyography activity of the lower limb muscles was recorded during running with and without anti-Pronation insoles. Two-way ANOVA with repeated-measures test was used for statistical analysis.
Results: The results indicated greater vastus medial frequency content in ACLR with the pronated feet group than that the healthy group during the loading phase (p=0.024). Also, results demonstrated that the rectus femoris frequency content was greater at the insole condition compared with and without it during the loading phase (p=0.014).
Conclusion: The results of this research showed a significant change in the activity of the quadriceps, hamstrings, gluteus medius, and gastrocnemius when using anti-pronation insoles in people with anterior cruciate ligament reconstruction and pronate feet. Therefore, it could be useful clinically.
Narges Chitsaz, Ahmad Reza Meamar, Elham Razmjou, Soheila Shafaghi-Sisi, Maryam Alipour, Maryam Sadeghi, Zahra Rampisheh, Zeinab Ghasemi, Rasoul Aliannejad, Alireza Badirzadeh,
Volume 23, Issue 4 (1-2024)
Abstract
Background: Pneumocystis jirovecii (P. jirovecii) causes Pneumocystis pneumonia (PCP) in people, especially the immunocompromised ones. It is also one of the serious causes of numerous lung problems in affected patients. Since documented data about P. jirovecii is not available in patients with pulmonary infections in Tehran, this study aimed to investigate the molecular epidemiology and parasitology of Pneumocystis to determine the frequency of the organism infection.
Methods: Bronchoalveolar lavage (BAL) samples were collected for 367 patients hospitalized in the lung department of Shariati Hospital in Tehran from July 2022 to July 2023. The samples were analyzed using Giemsa staining and molecular methods. After DNA extraction from samples, Nested polymerase chain reaction (Nested PCR) was employed for the amplification of the 18SrRNA gene and identification of P. jirovecii. The PCR products of Nested PCR were sequenced for final confirmation.
Results: Out of 367 samples, only one sample (0.27%) and 28 samples (6.7%) were found to be positive through parasitology and NestedPCR analysis, respectively. P. jirovecii was detected in seven (25%) and 21 (75%) immunocompromised and immunocompetent patients, respectively. Fever, shortness of breath and dry cough were the most common clinical symptoms among patients with Pneumocystosis. Patients with pulmonary disorders are prone to colonization by pneumocystis, which increases the risk of pneumocystosis and makes them a reservoir for transmission to susceptible people.
Conclusion: It can be concluded that patients with distinct lung disease are prone to colonization by Pneumocystis and, importantly, are at risk of infection. Also, according to the current study, Nested PCR was a suitable method for detecting P. jirovecii organisms because it had a very high sensitivity and specificity.
Samaneh Alijanian, Masoumeh Asle Rousta, Golnaz Asaadi Tehrani,
Volume 23, Issue 4 (1-2024)
Abstract
Background: Many scientific Researches have shown that diethylnitrosamine, which is used to induce liver carcinoma, has destructive effects on the kidney. Menthol is a monoterpene type with antioxidant, anti-inflammatory, and anti-cancer effects. In the current study, we investigated the effect of menthol on the expression of tumor-related factors and HIF-1α/VEGF signaling in the kidney cells of mice receiving diethylnitrosamine.
Methods: In this research, 16 male mice at the age of 14 days were divided into four groups including Control, Menthol, Nitrosamine, and Nitrosamine-Menthol groups. Nitrosamine and Nitrosamine-Menthol groups received diethyl-nitrosamine intraperitoneally (25 mg/kg) at the age of 14 days. Menthol and Nitrosamine-Menthol groups also received menthol by gavage (50 mg/kg) three times a week for 6 consecutive months. At the end of this period, the expression level of SFRP1, VHL, CTNNB1, HIF-1α, and VEGF in the kidney cells was measured using real-time PCR method.
Results: Menthol treatment caused a significant increase in the expression level of SFRP1 (P=0.021) and VHL (P=0.013) and a significant decrease in the expression level of CTNNB1 (P=0.001), HIF-1α (P=0.000) and VEGF (P=0.000) in the kidney cells of Nitrosamine-Menthol treated group compared to the Nitrosamine group.
Conclusion: The results of this study showed that menthol prevents the decrease in the expression of tumor suppressor factors and the increase in the expression of tumor stimulating factors and factors effective in angiogenesis in the kidney of mice treated with diethyl-nitrosamine, so menthol is probably useful in prevention and treatment of renal cancer.
Saba Ramezanzadeh, Golam Reza Barzegar, Hamid Oveisi Oskouei, Majid Pirestani, Mahmoud Mahami-Oskouei, Farzaneh Jafarian, Ehsan Ahmadpour, Seyed Abdollah Hosseini,
Volume 23, Issue 4 (1-2024)
Abstract
Background: Opportunistic pathogens such as Cryptosporidium, Isospora belli, Blastocystis, etc. cause various gastrointestinal and non-digestive diseases in people with HIV. These symptoms are especially severe in people with HIV who have a CD4 count of less than 200. This study aimed to determine the prevalence of parasitic infections in people living with HIV in Tabriz.
Methods: This cross-sectional and descriptive study was performed on137 people with HIV referred to Behavioral Disease Counseling Centers in Tabriz, 2019-2021. Then, after receiving written consent, fecal samples were collected and evaluated for the detection of parasitic infections using direct methods, Ziehl-Neelsen and Trichrome Weber stain.
Results: A total of 137 stool samples were collected, including 93 males and 44 females. Most of them were in the age range of 20-60 years. The overall frequency of parasitic infections was 57.7% and the highest prevalence was related to Blastocystis 24.1% and Cryptosporidium 14.6%.
Conclusion: Due to the relatively high prevalence of parasitic infections, especially Blastocystis and Cryptosporidium in people with HIV in Tabriz, which can endanger the health of these patients, essential interventions, including personal hygiene training to control and prevent infection with these pathogens, seem to be necessary.
Soroush Nasuhi, Abdolali Moshfe, Nasir Arefkhah, Ahmad Abolghazi, Aminallah Saadatnia,
Volume 23, Issue 4 (1-2024)
Abstract
Background: Toxocariasis is one of the most common diseases among humans and animals. According to the various studies, the rate of soil contamination with the worm's eggs in the world has been reported on average at 13.2% and in Iran at between 3.5 and 63.5%. Due to a lack of information about the status of infection with this worm in animals and humans in Yasuj city and also the importance of toxocariasis, this study was conducted to investigate the soil contamination of the Yasuj public parks with the eggs of Toxocara spp.
Methods: This descriptive study was performed on 150 soil samples from 6 parks in Yasuj in 2021. Samples were collected from five points based on geographical directions at a distance of 2–5 cm above the soil surface and transferred to the parasitology laboratory. To see the eggs of Toxocara species, a flotation method with saturated sugar water, and a light microscope with a 40X were used.
Results: Out of 150 soil samples from six parks, 11 samples (7.33%) were found to be infected with Toxocara species eggs and 139 samples (92.67%) were not contaminated. In total, out of the six investigated parks, pollution was not observed in two parks (33.33%), but pollution was observed in four parks with a contamination rate of 8% in three parks and 16% in one park.
Conclusion: According to the results of similar studies in Iran, it is concluded that Toxocara infections in the soil of Yasuj parks are similar to the national average and require more care, in terms of fencing the parks and preventing the free entry of dogs and cats.
Aida Nahumi, Maryam Peymani, Asadollah Asadi, Arash Abdolmaleki, Yasin Panahi, Mohammad Ali Shahmohammadi,
Volume 23, Issue 4 (1-2024)
Abstract
Background: Identifying protein interactions is one of the main challenges in the fields of biostructure and molecular biology. Despite extensive progress, the exact patterns of protein-protein interactions are still unknown. The main goal of this study is to computationally evaluate the interactions of fibronectin-1 in the extracellular matrix of decellularized trachea and integrins in adipose tissue stem cells in order to provide the most accurate possible visualization of these interactions and their role in biological processes.
Methods: After decellularization of the sheep trachea through the detergent-enzyme method, histological evaluations and ultrastructure photography of the samples were done by scanning electron microscopy. Also, the simulations of fibronectin1 binding of extracellular matrix protein with integrin αvβ1 and α5β3 of stem cells derived from adipose tissue were investigated, and interaction energy analysis was applied to predict the structure of protein-protein complexes using the algorithms available in HDOCK and ClusPro servers.
Results: The findings indicated the preservation of extracellular matrix components and scaffold ultrastructure. Also, in order to find the most favorable connection states in terms of energy, some of them were reported as stable interactions among the top types of connections. This insight provides a valuable understanding of cell-matrix adhesion, migration, and signaling, with potential implications for therapeutic development.
Conclusion: The prepared scaffolds are ideal for engineering applications for which computational analysis and experimental data have been used for visualization of stable connection states with energy efficiency between fibronectin and integrin. Also, more studies on cell adhesion modeling in connection with tissue engineering science can provide a suitable field for the development of regenerative medicine in further studies.
Masoumeh Matin, Nahid Kianmehr, Abbas Tabatabaiee, Mehrnaz Kajbafvala, Marzieh Yassin,
Volume 23, Issue 4 (1-2024)
Abstract
Background: The main purpose of this study was to investigate the effects of dry needling on pain and pain pressure threshold (PPT) in patients with non-specific chronic low back pain (NSCLBP).
Methods: 29 patients with NSCLBP were randomly allocated into two experimental (N=14) and control (N=15) groups. The dry needling intervention was considered for quadratus lumborum, gluteus medius, and lumbar multifidus, in 6 sessions, and subjects of the control group followed their routine lifestyle. The measurements included pain and PPT, which were evaluated in the pre-test, post-test, and follow-up periods. The pain was a variable that had a three-month follow-up assessment. Analysis of variance of combined measurement with repeated measurement and Benferoni's post hoc test were used to investigate the effect of the intervention on the outcome measurements.
Results: Three sessions of trigger point dry needling did not lead to a significant change in pain. However, after five sessions of dry needling, a significant decrease in pain was observed (p<0.05), and the positive changes significantly remained until one and three months after the end of the intervention (p<0.05). Furthermore; significant changes in PPT scores were detected following five sessions of trigger point dry needling treatment (p<0.05). These changes remained constant in the one-month follow-up assessment (p<0.05).
Conclusion: Dry needling for the trigger points of the QL, GM, and LM muscles can improve pain intensity and PPT in patients with NSLBP.
Soheila Fakhrian Roghani, Allahyar Arabmomeni,
Volume 23, Issue 4 (1-2024)
Abstract
Background: Obesity is a major health problem worldwide. Therefore, the purpose of this study was to determine the effects of l-carnitine supplementation on physical factors related to health and lipid profiles in obese female children with basic gymnastics exercises.
Methods: In this quasi-experimental study, with a pre-test-post-test design and a placebo group, 60 obese children among the girl students of Isfahan with BMI=30.28 kg/m2 were selected purposefully and were divided into 4 groups (Basic gymnastics training, L-carnitine consumption, Combined, and Placebo). The training protocol was implemented for 12 weeks, three times a week, and 45 minutes for each session. Furthermore, the groups of L-carnitine consumption and combined used L-carnitine tablets with a dose of 2 g per day for 12 weeks. To analyze the data, multivariate covariance, and post hoc Tukey tests were used.
Results: A significant increase in Cardio-respiratory endurance, muscular endurance, muscular strength and flexibility was observed in both basic gymnastics training and combined groups (p≤0.001). In addition, a significant decrease in total cholesterol, triglyceride, and LDL and a significant increase in HDL were observed in the basic gymnastics training, and combined groups (p≤0.002). Also, the changes caused by the combined group on all variables were significantly better than other groups.
Conclusions: These findings indicate the beneficial effects of basic gymnastics training along with L-carnitine supplementation on physical factors related to health and lipid profile in obese children. Therefore, the combined use of basic gymnastic exercises and L-carnitine supplementation can be useful to improve these variables.
Yousef Mohammadi-Kebar, Ahad Azami, Aziz Kamran, Erfan Sadeghilar,
Volume 23, Issue 4 (1-2024)
Abstract
Background: Systemic lupus erythematosus is an autoimmune disease in which organs and cells are damaged in the initial stages by tissue-binding autoantibodies and immune complexes. Clinical, demographic and laboratory information of patients with SLE in Ardabil province is the aim of this study.
Methods: The present study is a cross-sectional descriptive study that examines demographic data (age, gender), clinical information such as the first manifestation of the disease, clinical symptoms, etc., and laboratory findings such as CBC, ESR, CRP, proteinuria, FANA, Anti dsDNA, C3, C4, Anti RO and APS Ab of patients who referred to the rheumatology clinic of Imam Khomeini Hospital (RA) in 2021.
Results: Information related to the clinical characteristics of 50 patients, including 46 women (92%) and 4 men (8%), with an average age of 12.14 ± 40.78 years (range: 21-73) and age at diagnosis of 10.62 ± 31.76 years were investigated. The most common initial complaint in the studied patients was related to isolated joint symptoms (28%). The most common clinical symptoms observed in the examined patients were related to joint symptoms along with sensitivity to light with a frequency of 20%. ANA, anti-dsDNA tests positivity and reduced C3 and C4 levels were observed in 88%, 70% and 26% of patients, respectively.
Conclusion: The establishment of a registry system for the structured registration of information of patients with systemic lupus erythematosus in each region is necessary to a better understanding of the characteristics of the disease and better management policy of patients.
Hamid Vasfi, Hamid Mohebbi, Marefat Siahkouhian, Lotfollah Rezagholizadeh, Abuzar Jorbonian,
Volume 24, Issue 1 (4-2024)
Abstract
Background: Weight gain and obesity are the global health problem. Time-restricted feeding is a relatively new method of weight loss with many metabolic and anthropometric benefits. Low-intensity aerobic exercise also has beneficial effects on weight loss and metabolic factors; However, the combined effect of TRF and low-intensity aerobic exercise on metabolic and anthropometric factors in healthy overweight middle-aged men has rarely been investigated. The present research has investigated this purpose.
Methods: Among 55 volunteers, 27 healthy middle-aged men in the age (35 to 55) with overweight (25 The groups included: 1-TRF18:6 & EX 2-TRF 16:8 & EX 2-TRF 14:10 & EX. Three groups performed TRF protocols with different feeding times (6, 8 and 10 hours of free feeding) and fasting in the remaining 24 hours, and the same low -intensity aerobic exercise (55-65% of maximum heart rate). The interventions continued for 12 weeks. Blood and body composition tests were measured before and after the intervention. Daily food intake was measured before the intervention and during 12 weeks. Finally, all the data were analyzed.Analysis of variance with repeated measures was used for analysis. P values less than 0.05 were considered as a significant criterion in all statistical tests.
Results: The combination of TRF and exercise decreased glucose and insulin resistance in all groups. Cholesterol and LDL decreased in the 6-h and 8-h TRF groups. HDL reduction was significant only in the 8-h TRF group. Weight and BMI were reduced in the 6-h and 8-h TRF groups. The percentage of fat reduced in the 6-h group. There was no significant difference between the groups for any of the variables. The average energy received in 12 weeks was not significantly different in any group; however the 6-h model caused a greater decrease in the average energy received during 12 weeks than in the other two groups.
Conclusion: The 6-h TRF model along with light aerobic exercise is suggested as the most reliable method to improve the metabolic and anthropometric profile in overweight middle-aged men.
Elina Barazesh, Mostafa Govahi, Mojtaba Ranjbar,
Volume 24, Issue 1 (4-2024)
Abstract
Background: Ginkgo biloba is a plant with many therapeutic characteristics because it’s rich in polyphenolic contents. This study was done to evaluate the antibacterial activities of Ginkgo and to compare the antibacterial potential of ethanolic, methanolic and aqueous leaf extracts of Ginkgo biloba.
Methods: The antibacterial activity of Ginkgo extracts was evaluated using a disc diffusion method against four strains of bacteria: Escherichia coli, Salmonella typhimurium, Bacillus subtilis, and Staphylococcus aureus. The Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of the extracts were assessed.
Results: The results indicated that all extracts of Ginkgo possess distinguished antibacterial activity. Among them the methanolic extract exhibited maximum antibacterial activity and the aqueous extract showed minimum antibacterial activity. The highest MIC and MBC were determined at 3.13 and 6.25 µg/mL of aqueous extract against Bacillus subtilis, respectively.
Conclusion: Regarding the acquired results from this study, the leaves of Ginkgo biloba possess a considerable amount of antibacterial activity that can make them one of the most valuable antibacterial resources that could be used in food and therapeutic industries. Therefore, more studies should be conducted on this plant.
Reza Namadkolahi, Avissasadat Meraji, Safa Yaltaghiani, Somayeh Hekmatfar,
Volume 24, Issue 1 (4-2024)
Abstract
Background: Early childhood caries is a disease that affects the quality of life of a child and his overall health; it can interfere with the child's daily activities and growth process. This disease is affected by various factors such as diet, oral hygiene, social health factors and psychological issues. This study aimed to investigate the relationship between early childhood caries in children and the parents’ quality of life.
Methods: This cross-sectional descriptive study was conducted on 552 children aged 12 to 71 months in Ardabil city. The convenient sampling method was used and participants were patients who referred to Ardabil Dental Health Center. Dmft index (decayed, missing, and filled teeth) was assessed after examining the children. The World Health Organization questionnaire was used to evaluate the quality of life of the parents. The demographic information of children and parents was also determined. Statistical analysis included, kruskal-wallis, t-test and linear regression.
Results: The average score of the quality-of-life questionnaire was 66.42±17.91. No significant correlation was found between the mean quality of life of parents and dmft index. (p=0.916, r=0.008) A significant difference was observed between the child's dental caries and the father's age and education. (p<0.05) There was no significant difference in the mean dmft index, 5.32±0.5 in boys and 5.85±4.7 in girls. (p=0.459)
Conclusion: This study showed that the general quality of life of parents and its sub-branches such as the place where the child lives, the social and physical health of the parents are not directly related to early dental caries in children.
Maryam Nazari, Nilofar Saeli, Mohsen Arzanlou, Saghar Jafari-Ramedani, Hafez Mirzanejad-Asl, Farzad Khademi, Aida Alinezhad,
Volume 24, Issue 1 (4-2024)
Abstract
Background: Antibiotic resistance represents a critical global concern within the medical community, posing significant challenges in the treatment of infections caused by drug-resistant pathogens. Over the years, broad-spectrum fluoroquinolones have been extensively used to treat infections caused by both Gram-positive and Gram-negative bacteria, including Pseudomonas aeruginosa. In this study, we decided to assess the prevalence of plasmid-mediated quinolone resistance mechanisms among clinical isolates of P. aeruginosa in Ardabil hospitals.
Methods: We analyzed a total of 200 clinical isolates of P. aeruginosa, collected between June 2019 and May 2023. The antibiotic resistance profiles of these strains against various fluoroquinolone antibiotics were determined using the disk diffusion method. Additionally, we investigated the presence of qnrA, qnrB, qnrC, qnrD, and qnrS genes through polymerase chain reaction (PCR) analysis. Furthermore, we assessed the expression levels of efflux pump genes and outer membrane porin genes using the quantitative reverse transcription PCR (qRT-PCR) in fluoroquinolone-resistant P. aeruginosa strains.
Results: Our findings revealed that 69% of P. aeruginosa strains were resistant to fluoroquinolones. The resistance rates for different fluoroquinolones were as follows: ciprofloxacin 55.5%, ofloxacin 62%, norfloxacin 53.5%, lomefloxacin 55.3%, and levofloxacin 55.5%. Notably, 78.9% of these strains exhibited multidrug resistance (MDR). Among the qnr genes, qnrB was the most prevalent (2.9%). No other qnr genes were identified. Interestingly, 75% of P. aeruginosa strains carrying the qnrB gene showed overexpression of efflux pump genes, while 100% exhibited down-regulation of the oprD gene.
Conclusion: Given the high prevalence of fluoroquinolone-resistant P. aeruginosa clinical isolates in Ardabil hospitals and the multifactorial nature of resistance, continuous monitoring of antibiotic resistance trends and understanding the underlying resistance mechanisms are crucial for selecting appropriate treatment strategies.
Taher Sadeghian, Saeideh Allahyari,
Volume 24, Issue 1 (4-2024)
Abstract
Background: Oral mucositis as a complication of chemotherapy has posed significant challenges to the quality of life in cancer patients. Tricyclic antidepressants, including nortriptyline can control the pain caused by mucositis by inhibiting sodium channels. Since patient compliance and rapid drug effect are the goals of the proposed formulation, it was decided to prepare and evaluate a nortriptyline mouthwash according to the United States Pharmacopeia.
Methods: Based on the pharmacopeia, the necessary excipients in the nortriptyline mouthwash were determined, and the amount of each was specified. After validating the UV spectrophotometric technique, drug interaction with the mouthwash container was investigated. In addition to measuring the aqueous activity, necessary microbiological tests were also performed.
Results: The UV spectroscopy method validation for nortriptyline was performed at the obtained maximum absorption wavelength (238.5 nm). The findings of the investigation of the interaction of polyethylene terephthalate container with nortriptyline indicate almost no drug adsorption into the internal part of the mouthwash container over 28 days. The results of antibacterial and antifungal/antimycotic tests were within the standard range of the United States Pharmacopeia. The mouthwash's aqueous activity was also found to be 0.81, indicating an unfavorable environment for microbial growth.
Conclusion: This study has shown that the prepared nortriptyline mouthwash meets microbiological resistance standards and there is no interaction between the active drug ingredient and the proposed container. Therefore, this formulation can be suggested as a promising candidate for clinical exploration.
Sheida Payamipour, Maghsoud Peeri, Mohammad Ali Azarbaijani, Forouzan Fattahi Masrour,
Volume 24, Issue 1 (4-2024)
Abstract
Background: Rheumatoid arthritis is an autoimmune disease that gradually destroys the synovial joints and leads to chronic inflammation. This autoimmune disorder is associated with an increase in stress-related symptoms, including anxiety and depression, and a decrease in social behaviors, which severely reduces the patient's quality of life. Previous studies show that higher physical activity during adolescence may lead to a decrease in neurobehavioral disorders in adulthood. This study was conducted with the aim of evaluating the increase of physical activity from early adolescence on stress-related behaviors and inflammation in mice with rheumatoid arthritis.
Methods: Male mice were randomly exposed to a running wheel from early adolescence to adulthood, and then rheumatoid arthritis was induced. Stress-related behaviors, including anxiety, depression and social behavior were investigated in animals. The level of interleukin 17 (IL-17) and interferon gamma (IFN-γ) were also measured as important inflammatory markers in the serum.
Results: The results showed that physical activity from early adolescence significantly resulted in decreased stress in mice with rheumatoid arthritis. In addition, the running wheel led to a decrease in the levels of (IL-17) and (IFN-γ) in rheumatoid arthritis-induced mice as two important indicators of inflammation.
Conclusion: This study shows that increased physical activity during adolescence to adulthood may make mice resistant to increased stress and inflammation caused by rheumatic arthritis.
Mehrnoosh Kaviani, Mouhamad Hassan Pourrashid, Saeedeh Galledar,
Volume 24, Issue 2 (7-2024)
Abstract
Background: Antibiotics are commonly prescribed drugs by dentists. Irrational use of these drugs can lead to unwanted drug side effects, such as drug resistance in addition to unnecessary costs. This research was conducted to determine the antibiotic prescription pattern by dentists of Ardabil City based on the international ATC/DDD system.
Methods: In this cross-sectional descriptive study, 250 prescriptions of patients referred to dentists in Ardabil during the second quarter of 2021 were examined. The information related to prescribed drugs includes: drug dose, frequency of drug use, number of drugs that were collected and completed in the dental offices and clinics of Ardabil city, and finally after converting the data into a certain daily dose based on the ATC-DDD system, acquired data was analyzed using One-way analysis of variance and Tukey's post hoc tests and SPSS version 21 software, the significance level was considered less than 0.05.
Results: Out of the total 250 reviewed prescriptions, 207 prescriptions (82.8%) contained antibiotics. Metronidazole (73.4%) and amoxicillin (70.5%), were the most antibiotic drugs prescribed by Ardabil dentists respectively. In all prescribed drugs, the DDD value was higher than the standard DDD value. There was no significant difference between men and women in terms of the number of copies (p<0.05). The highest frequency was related to the prescription of three drugs and the lowest frequency was related to the prescription of six drugs. In terms of pharmaceutical forms, 201 prescriptions (97.1%) were only oral drugs and 6 (2.9%) were both oral and injectable
Conclusion: The results showed that the amount of antibiotics prescribed in prescriptions was higher than the standard DDD and the number of drugs prescribed in prescriptions was also higher than the figure recommended by the World Health Organization.
Morad Beiranvand, Hossein Hamidinejat, Somayeh Bahrami, Mohammad Reza Tabandeh, Meysam Makki,
Volume 24, Issue 2 (7-2024)
Abstract
Background: A zoonotic obligate intracellular protozoan parasite, Toxoplasma gondii, infects all warm-blooded animals as well as humans worldwide. Identification of the level of infection in intermediate hosts gives us an important data about understanding the role of this parasite in human health as well as estimating the economic loss in livestock. Therefore, the main aim of this study was the isolation and identification of T. gondii from aborted goat fetuses by PCR in Lorestan province.
Methods: From autumn 2023 to summer 2024, the brain and liver of 100 goat fetuses were examined for T. gondii by PCR based on the amplification of 529 base pair fragments from repetitive regions of the parasite genome. The study was performed in three aborted fetus groups, less than 2 months, 2 to 4 months and more than 4 months.
Results: From a total of 100 examined samples, conventional PCR detected the T. gondii infection in 6 (6%) and 2 of the brain and liver fetuses respectively.
Conclusion: This study shows a notable level of infection in goat fetuses, and as a result, T. gondii should be considered an important agent involved in the abortion of goats in the Lorestan province of Iran. On the other side, it is necessary to pay more attention to the risk of transmission of this parasite from farmed animals to humans, especially pregnant women and people with suppressed immune system.
Roksana Farabi, Aylin Javan-Molaei, Esmaeil Farzaneh, Saeid Sadeghieh Ahari, Sara Mostafalou,
Volume 24, Issue 2 (7-2024)
Abstract
Background: Poisoning is one of the common causes of hospital emergency department referrals, especially in adolescents and young people; therefore, knowing the pattern of poisoning will be helpful in identifying risk factors and early detection of poisoning. The aim of this study was to investigate the epidemiology of acute poisoning in adults referred to Imam Khomeini Hospital in Ardabil from 2017 to 2020.
Methods: In this descriptive-analytical and retrospective study, 755 cases related to poisoned patients referred to Imam Khomeini Hospital in Ardabil from 2017 to 2020 were reviewed. The extracted data include ward, gender, age, marital status, place of residence, general status, cause of poisoning, and time of referral, were recorded on the checklist. Obtained Data were analyzed by Chi-square test and SPSS25 software.
Results: The results showed that drug poisoning, opium (opium and its derivatives) and pesticides were the first to third ranks of the most poisoning agents. Most of the poisoned patients were men, married, in the age group of 21 to 30 and living in urban areas. Most of the poisonings were intentional and 33 deaths (4.4%) happened due to poisonings. The cause of poisoning had a significant relationship with age, sex, marital status, place of poisoning (p<0.05) but had no significant relationship with the general condition and time of patients' referral.
Conclusion: Acute poisoning is a major health problem in Ardabil province. The results show that the three agents of drug poisoning, opium and pesticides are the main means of poisoning in adults. Most cases of poisoning occurred in young and adult groups and were in the age range of 21 to 30 years. Poisonings occurred in people aged 31 to 40 were more due to amphetamine. Poisoning with aluminum phosphide tablets had increased compared to before. Consumption of aluminum phosphide is one of the problems of today's societies and it was one of the main causes of death in this research, so it is better to provide necessary measures for management and training for its correct use. Also, by limiting the use of these agents and replacing other methods and techniques, poisoning with these agents can be reduced.
Reza Javanshir, Hossein Kalarestaghy, Ramin Salimnejad,
Volume 24, Issue 2 (7-2024)
Abstract
Background: Premature ovarian insufficiency (POI) is the cessation of ovarian function in women under 40. One of the mechanisms involved in the induction of POI is oxidative stress. This study aims to investigate the effect of caffeic acid on the morphometrical changes of ovarian follicles of POI model mice.
Methods: Thirty-two female mice were randomly divided into 4 groups (n=8): 1) Control group (cont), 2) Caffeic acid group (CAF), 3) Premature ovarian insufficiency group (POI), and 4) Premature ovarian insufficiency + Caffeic acid group (POI+CAF). POI was induced through daily subcutaneous injections of D-galactose (200 mg/kg) for 6 weeks. Caffeic acid (60 mg/kg, intraperitoneal) was injected daily for 4 weeks from 15th day. One day after the last injection, the mice were anesthetized and the ovaries were removed. Then, the morphometric changes of ovarian follicles were examined using hematoxylin-eosin (H&E) staining.
Results: The results showed that D-Galactose-induced POI significantly decreases the diameter of primary, secondary, and antral follicles (P<0.05). Administration of caffeic acid in the POI+CAF group significantly prevented the reduction of follicle diameter (P<0.05).
Conclusion: The results showed that caffeic acid can prevent the reduction of the diameter of ovarian follicles in POI model mice.
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