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Showing 46 results for Ros
Gholamreza Irajian , Reza Mirnejad, Mohammad Reza Jalali Nodoshan, Nafiseh Ghorbanpour,
Volume 13, Issue 1 (4-2013)
Abstract
Background & Objectives: Prostatitis is relatively one of the common diseases in elderly men. Treatment of this disease is difficult and because of frequent relapses, it provides complicated problems for patients and the physicians. Detection of reservoirs and determination of prevalence of involved microbes in prostatitis are important in the epidemiology and control of the disease. This study was conducted to determine the prevalence of Mycoplasma genitaliumin patients with prostatitis by sequencing and PCR-RFLP techniques. Methods : In this cross-sectional study, 200 paraffin-embedded prostate samples from patients with prostatitis during 2008-2011 were checked for M. gentialium. After cutting the tissues and homogenization, the genomic DNA was extracted and used as template in PCR. Primers targeting a 465 bp regions of 16S rRNA of M. genitalium were used in the assay. PCR products were sequenced and Cac8I, Bbs I, EcoRI, AluI, TaqI endonucleases were used in RFLP analysis. Results : Of 200 samples, 4 were positive in PCR. The results of DNA sequencing and RFLP confirmed the amplified genes corresponded to M. genitaliumG37. Conclusion : The Mycoplasma genome present in tissue samples of prostate showed this bacterium could be one of the risk factors for prostatitis in men. However large studies and control groups are needed to prove this finding.
Masoud Entezariasl , Khatereh Isazadehfar, Zahra Noorani,
Volume 13, Issue 2 (7-2013)
Abstract
Background & Objectives: Postoperative nausea and vomiting (PONV) is one of the most common complications of anesthesia and without prophylactic intervention occurs in about one-third of patients under general anesthesia. The aim of this study was to compare the efficacy of ondansetron and metoclopramide in reducing PONV after laparoscopic cholecystectomy. Methods : In this study, 90 patients undergoing laparoscopic cholecystectomy were randomly allocated into three equal groups (n=30) and in the first group 10 mg metoclopramide, in the second group 4 mg ondansetron and for placebo group 2 cc normal saline preoperatively were injected. Anesthesia complications in recovery and nausea and vomiting in recovery and 6 hours and 24 hours after surgery were evaluated. Data were analyzed by SPSS software with chi-square test and analysis of variance (ANOVA). P<0.05 was considered significant. Results: The incidence of nausea in placebo group was 66.7 %, in metoclopramide group was 43.3 % and in ondansetron group was 33.3 %. The difference only between placebo and ondansetron groups was significant (p=0.019). The incidence of vomiting in placebo group was 56.7%, in metoclopramide group was 20% and in ondansetron group was 26.7% and there was significant difference between placebo and ondansetron groups (p=0.035) and between placebo and metoclopramide groups (p=0.007), whereas there was not any significant difference between intervention groups (p=0.12). Also anti-emetic drug administration in ondansetron group (40%) in comparison with metoclopramide (63.3%) and placebo (66.7%) was lower, but this difference was not statistically significant (p= 0.07). Conclusion: For prevention of vomiting after laparoscopic cholecystectomy, both metoclopramide and ondansetron are effective. In comparing these two drugs, in preventing of nausea ondansetron is more effective than metoclopramide, whereas there was not any significant difference between two drugs in preventing of vomiting.
Seyied Hamid Barzgar, Mohammad Ali Mohseni, Ali Goudarzi, Ali Tabrizi,
Volume 13, Issue 3 (10-2013)
Abstract
Introduction : Meniscal tears are one of the most common injuries treated by arthroscopic techniques. Arthroscopic meniscal repair is an accepted way of treatment for meniscal tears. Different arthroscopic techniques for meniscal repair are: inside-out, outside-in and all inside. In the first 2 techniques, meniscus is repaired by sutures and in the later by suture or by commercial ready implants . The goal of current study is assessing clinical results of arthroscopic repair of longitudinal meniscal tears with vertical cruciate double mattress sutures by outside-in technique after 9 months. Methods: In this case series study, in 13 patients having criteria for engaging the study with longitudinal isolated meniscus tear, arthroscopic meniscal repair was done with vertical cruciate double mattress sutures by outside-in technique and patients were followed for 9 months. Results: Of 13 patients, there were 12 males (92.3%) and one female (7.7%) aged 15-38 (average 28.3) years. In follow up period, there was one case (7.7%) of irritation by subcutaneous knot. There was not any failure of repair. Average Lysholm score increased from 55.23 to 91.23 after 9 months of follow up, which was statistically significant (p<0.001). At the end of follow up period, there was not any medial joint line tenderness of knee, giving way or significant effusion or pain. Conclusion: This study shows that this technique has a good short term outcome with no failure and low complications but it is necessary to do more long term studies to prove it.
Fereshteh Sharifi Ferdoey , Shiva Irani, Mojghan Zandi, Masoud Soleimani,
Volume 14, Issue 3 (10-2014)
Abstract
Background & objectives : T he main goal of tissue engineering is regeneration and restoration of damaged tissues and organs, besides being used in medicine . Scaffolds are the main segments for tissue engineering, and plasma surface modification is one of the modern methods used for surface modification on polymer scaffolds. The aim of this study was to evaluate the effect of nano-fibers with different densities on fibroblasts' behavior besides the plasma surface modification. Methods : Poly Ɛ-Caprolactone nano-fibers (PCL) were developed by an electro-spinning technique at different collecting times. These nano-fibers were then modified by oxygen plasma. Cellular attachment to the nano-fiber and their morphology were evaluated using scanning electron microscope (SEM) and cellular activities were also studied by 3-[4,5-dimethylthiazol- 2-yl]-2,5 diphenyltetrazolium bromide (MTT). Scaffold biocompatibility test was assessed using inverted microscope. Results : Scanning electron microscope images of nano-fibers showed that increase in time of spinning has significantly heighten fiber density, on the other hand plasma surface modification of nano-fibers had significant effects on their respective biocompatibilities. The result of cell culture showed that nano-fiber could support the cellular growth and replication by developing 3-dimensional topography. Conclusion : Our results showed that increase in time of spinning and using plasma surface modification of nano-fibers by oxygen plasma would result in providing surface with the highest similarity to the extracellular matrix.
Hashem Faal Moghanlo, Fatemeh Sadat Hosseini, Farzaneh Mikaili Manee,
Volume 14, Issue 3 (10-2014)
Abstract
Background & objectives : Different types of practises are known for improving motor skills in intellectually disabled boys. The purpose of this study was to compar e the impact of spark motor program and basketball on improving of gross motor skills in this people. Methods: In this semi-experimental study , from 98 educable intellectually disabled students who studied in special school in Urmia, 30 children ( age range of 9 to 13 years and IQ mean 64.4 ) were selected objectively and divided in three groups (2 experimental and 1 control) based on pre - test. BOTMP was used as a measurement of motor ability. Selected motor program (Spark motor program) including strengthening training, games, sports and basketball techniques was performed for 24 sessions. T-tests (dependent) and co-variance were used to comparison of results. Results: In Spark group after 24 sessions, there were significant effects on balance (p= 0.000), bilateral coordination (p=0.000) and strength (p=0.001). There was no significant effect in agility and speed (p= 0.343) in basketball techniques group after 24 sessions, there were significant effects in agility and speed (p= 0.001), balance (p= 0.000), bilateral coordination (p= 0.013) and strength (p= 0.007). Conclusion: Based on the results of this study, it can be claimed that the Spark program and basketball techniques improve gross motor skills in educable intellectually disabled students. We also found a significant difference between the Spark program and basketball techniques efficacy on the improved skills. Furthermore, the efficacy of Spark program was significantly higher than basketball techniques (p<0.05).
Sh Heydari Tajaddod, N Najafzadeh, M Mahdavi Rad, H Sheikhkanloui Milan, H Kalarestaghi, V Nejati,
Volume 15, Issue 2 (7-2015)
Abstract
Background & objectives: Hair follicle stem cells (HFSCs) are multipotent and various types of HFSCs were introduced. HFSCs separation using cell surface markers is one of the interesting strategies in the replacement of old methods. In this study, we used magnetic activating cell sorting (MACS) to separate HFSCs.
Methods: In this study, HFSCs were isolated from Balb/c mice and dissected under an invert microscopy, and bulge area isolated and the bulge cells cultivated about 14 days. The CD34 positive cells isolated using CD34 monoclonal antibody and magnetic activated cell sorting system (MACS), then the cells incubated in DMEM/F12 and 10% FBS. The CD34 positive cells counted using a neubauer slide and evaluated under a fluorescent microscopy.
Results: Here, we isolated CD34 positive cells using MACS and 12±1. 04% of HFSCs were CD34 positive and we found that, CD34 positive cells survived during 7 days cell culture in vitro.
Conclusion: The results show that MACS is useful to increasing density gradient of cells in vitro.
Seyedmahdi Banan Khojasteh , Elaheh Basirat, Farzam Sheikhzadeh , Homeira Hatami,
Volume 17, Issue 1 (4-2017)
Abstract
Background & aim: Nowadays because of difficulty in the supply and injection of insulin and blood sugar reducing drugs and considering the side effects of chemical drugs, researcher’s attention is drawn to using herbal medicines. Liver is one of the organs affected by diabetes in different functional aspects. The purpose of this research was studying the effect of Rosa canina extract on reducing the adverse effects and improving diabetes symptoms related to histophysiology, amount of biochemical serum factors and liver enzymes in male rats.
Methods: 32 male Wistar rats, weighed 200-220 gr, were divided into 4 groups. Diabetes was induced by intraperitoneal injection of STZ (60mg/kg). The period of experiments was 4 weeks. At the end of experiments, the rats were sacrificed and their serums were collected for measurement of liver enzymes. Also, histological sampling of liver was done. Statistical analysis was performed using analysis of variance (one-way ANOVA).
Results: The results of this study showed that treatment with ethanol extract of Rosa canina led to significant reduction of liver enzymes in the Rosa extract-treated groups in comparison with the diabetic group, indicating the protective effect of alcoholic extract of Rosa canina on liver tissue against diabetes-induced damages. In liver histopathology, a distinct fat change in the lobular center areas was created in the diabetic group. Pathological changes were not significant in the liver of the Rosa extract-treated groups; liver in this group was relatively healthier than in diabetic group.
Conclusions: Rosa extract improved this organ's function in STZ-induced diabetic rats by reducing serum biomarkers of liver pathology and reducing histological damages.
Elshan Bazhan , Behzad Baradaran,
Volume 17, Issue 2 (7-2017)
Abstract
Background & objectives: Prostate cancer is one the most common cancer in men whose incidence is increasing in many countries. According to the studies, decreased expression of miR-143 has been reported in prostate cancer. In this study, we replaced miRNA-143 in prostate cancer cells by vector based miRNA-143 and evaluated its inhibitory effects on migration of prostate cancer cells (PC3).
Methods: MTT assay was performed to reach an inhibitory concentration of Genticin antibiotic (G418 (in PC3 cells. Then, miRNA-143 vector was transfected into PC3 cells via JetPEI transfection reagent. The transfected cells were selected by G418 antibiotic according to a 2-week treatment with IC50 concentration. Then, the expression level of miRNA-143 was measured by qRT-PCR method. To evaluate the effect of miRNA-143 in inhibition of migration, scratch wound healing assay was performed.
Results: Results of MTT assay showed the IC50 level of G418 on PC3 cells was obtained to be 141.9 μg / ml. The results of qRT-PCR indicated increased expression of miRNA-143 in PC3 cells transfected with miRNA-143 compared to control cells. Finally, the results of wound healing assay showed migration reduction in transfected cells compared with control cells (empty vector).
Conclusion: The results showed that miRNA-143 play an important role in cell migration during prostate cancer metastasis, and it can be a good candidate for molecular treatments.
Faiba Kahnamooi, Firooz Amani, Mahsa Vallaei , Mahnaz Azari,
Volume 17, Issue 4 (1-2017)
Abstract
Background & objectives: Therapeutic abortion in the second trimester of pregnancy can be done by a number of medical or mechanical methods. The aim of this study was to compare the efficacy of vaginal misoprostol with and without letrozole and combination of misoprostol and laminaria in preoperative cervical ripening among women with under second-trimester abortion.
Methods: In this interventional study, 120 pregnant women with the gestational age of 20 weeks, who were candidates for therapeutic abortion, were selected and randomly divided into three groups (n=40). Proper counseling was done and a written informed consent was obtained before starting the treatment regimen. The first group received vaginal misoprostol alone and the second group received misoprostol in combination with laminaria and oral placebo. In the third group, in addition to cervical placebo on the first and second days, letrozole was prescribed, and on the third day, the patients were hospitalized and received vaginal misoprostol and third dose of letrozole. The patients were regularly examined every 4 hours, and in the absence of abortion, vaginal misoprostol was repeated after 4 hours.
Results: The results showed that termination of pregnancy was caused by embryo anomaly in 23 cases and fetal death in 97 cases. In this study, response to treatment was 90% in the group receiving laminaria and misoprostol 85% in the group receiving letrozole and misoprostol and 67% in the group receiving misoprostol. Regarding the duration and amount of bleeding, the groups receiving laminaria and misoprostol had the lowest duration and severity of bleeding, but this difference was not statistically significant. Combination of misoprostol and laminaria caused a significantly shorter time interval from the initiation of intervention to abortion, and pain relief was also higher than the other two groups.
Conclusion: Based on the obtained results, a combination of misoprostol and laminaria is recommended for induction of second-trimester therapeutic abortion.
Elmira Gorbanzadeh, Mohammad Zaefizadeh ,
Volume 17, Issue 4 (1-2017)
Abstract
Background & objectives: Prostate cancer is one of the most common cancers in the world, and its prevention is expected to increase in the future appreciably. Following the emergence of the new field of nutritional genetics and investigations about the effect of natural antioxidants on the hypermethylation of genes, this study was performed to measure the effect of Vaccinium arctostaphylos poly phenols and anthocyanins on PC-3 cell line as well as methylation and expression changes of GSTP1 gene.
Methods: In order to evaluate the survival potency of PC-3 cell line in a completely randomized design, MTT test was used and these cells were treated with different concentrations of Vaccinium arctostaphylos extract (4500, 2250, 1125, 562.5, 140.62, 70.31, 35.15, 17.57, 8.78, 4.39 µg/ml) for 24, 48 and 72 hours. Expression level of GSTP1 gene was analyzed by Q-RT-PCR method in 2500, 1250, 625, 312 and 156 µg/ml concentrations of Vaccinium.
Results: Statistical results showed that Vaccinium arctostaphylos poly phenolic extract at 35-70 ppm concentration significantly reduced the survival rate of PC-3 cells. Moreover, compared to control cells, the expression level of GST gene significantly increased in PC-3 cells treated with 1250ppm extract.
Conclusion: Anthocyanin- polyphenolic extract from Vaccinium arctostaphylos can decrease the survival rate of cancerous cells and GST gene expression in human prostate cancer PC-3 cells. This may be explained by changes in cell carcinogenesis pathway or CpG demethylation process.
Keywords: Vaccinium arctostaphylos; Antioxidant; Cancerous Cells; Prostate.
Background & objectives: Prostate cancer is one of the most common cancers in the world, and its prevention is expected to increase in the future appreciably. Following the emergence of the new field of nutritional genetics and investigations about the effect of natural antioxidants on the hypermethylation of genes, this study was performed to measure the effect of Vaccinium arctostaphylos poly phenols and anthocyanins on PC-3 cell line as well as methylation and expression changes of GSTP1 gene.
Methods: In order to evaluate the survival potency of PC-3 cell line in a completely randomized design, MTT test was used and these cells were treated with different concentrations of Vaccinium arctostaphylos extract (4500, 2250, 1125, 562.5, 140.62, 70.31, 35.15, 17.57, 8.78, 4.39 µg/ml) for 24, 48 and 72 hours. Expression level of GSTP1 gene was analyzed by Q-RT-PCR method in 2500, 1250, 625, 312 and 156 µg/ml concentrations of Vaccinium.
Results: Statistical results showed that Vaccinium arctostaphylos poly phenolic extract at 35-70 ppm concentration significantly reduced the survival rate of PC-3 cells. Moreover, compared to control cells, the expression level of GST gene significantly increased in PC-3 cells treated with 1250ppm extract.
Conclusion: Anthocyanin- polyphenolic extract from Vaccinium arctostaphylos can decrease the survival rate of cancerous cells and GST gene expression in human prostate cancer PC-3 cells. This may be explained by changes in cell carcinogenesis pathway or CpG demethylation process.
Niloofar Sadeghi , Marzieh Tavalaee , Mohammad Hosein Nasr- Esfahani ,
Volume 18, Issue 1 (4-2018)
Abstract
Infertility, especially in the last decade, has been rising as a global problem, affecting approximately 10-15% of the world's population. The abundance and origin of various types of infertility are different. Studies have shown that reactive oxygen species (ROS) are involved in infertility, in particular in male infertility. Although ROS is essential for normal physiological functions of sperm such as capacitation, hyper activation, acrosome reactions, and binding to the oocyte, excessive levels can be one of the main causes of defective sperm function, which not only impairs the health of sperm DNA, but also affects fertilization via oxidation of proteins, in particular the fatty acids of the sperm cell membrane. Also, the elevated ROS levels facilitate sperm DNA damage, which leads to activation of apoptotic pathway and cell death. Therefore, the quality of semen is functionally reduced. Since the oxidative damage to sperm DNA is associated with both miscarriage and development abnormalities in the offspring, it is essential to find new strategies to recognize the cellular and molecular biology of sperm. Therefore, considering different mechanisms of oxidative stress affecting sperm can contribute significantly to the etiology of male infertility
Rahmatollah Parandin, Leili Mohammadi,
Volume 19, Issue 2 (7-2019)
Abstract
Background & objectives: In recent years, regarding the side effects of chemical drugs, the use of medicinal plants has increased due to their low side effects, low costs and effective compounds. The aim of this study was to investigate the anti-inflammatory, anti-nociceptive, and anti-pyretic effects of hydroalcoholic extract of Rosa canina L. fruit in male mice.
Methods: In this experimental study, 120 male BALB/c mice weighing 23-30 g were used. In each test, the mice were divided into 5 groups (in each group, n=6), including control group, positive control and three experimental groups treated intraperitoneally with hydroalcoholic extract of Rosa canina L. fruit at doses of 100, 200 and 400 mg/kg respectively. The anti-inflammatory and antipyretic activities were measured using xylene-induced ear edema and brewer’s yeast-induced pyrexia tests, respectively. In addition, the antinociceptive activity was measured using the abdominal constrictions induced by acetic acid and formalin tests. The data were analyzed by SPSS statistical software and One Way ANOVA test. The level of significance was set at p<0.05.
Results: 200 (p<0.05) and 400 mg/kg (p<0.001) doses of extract reduced significantly inflammation. Doses of 100 (p<0.05), 200 (p<0.01) and 400 (p<0.001 significantly reduced pain in the abdominal constriction test and dose of 400 mg/kg (p<0.05) decreased the chronic pain in formalin test. Extract treatment did not reduce fever in any of the existing doses.
Conclusions: The findings of this study suggest that Rosa canina L. fruit has anti-inflammatory and visceral analgesic activity, which may be due to its antioxidant potential.
Keyvan Amirshahrokhi, Behnam Mohammadi, Mansuor Miran, Elham Jabari,
Volume 19, Issue 2 (7-2019)
Abstract
Background & objectives: Nonsteroidal anti-inflammatory drugs (NSAIDs) - induced peptic ulcer disease is a common gastrointestinal disorder affecting many people worldwide. Rosa canina L. is a member of Rosa species that has been widely used as an herbal medicine in many countries. Rosa canina has therapeutic effects against various disorders through its antioxidant, anti-inflammatory and cytoprotective properties. The aim of the present study was to evaluate the potential beneficial effect of Rosa canina on indomethacin-induced ulceration in mice.
Methods: Experiments were performed on male Swiss albino mice. Indomethacin (80 mg/kg, orally) was used to induce gastric ulceration. The extract of Rosa canina fruits (200 and 400 mg/kg/day) was given orally five days before the administration of indomethacin. Normal mice were used as a normal control group.
Results: Macroscopic evaluation of gastric mucosa revealed that indomethacin administration (80 mg/kg) produced apparent gastric mucosal ulcers, while pretreatment with Rosa canina (200 and 400 mg/kg) effectively protected gastric mucosa against indomethacin induced ulcers. Rosa canina significantly increased the level of glutathione (GSH) and decreased the levels of malondialdehyde (MDA) and myeloperoxidase (MPO) in the gastric tissues compared with those in the control group.
Conclusion: Oral treatment with the Rosa canina fruit extract decreased the gastric ulcer induced by indomethacin through antioxidant and anti-inflammatory mechanisms
Somaieh Matin, Yousef Imani Marani, Hossein Salehzadeh,
Volume 19, Issue 3 (10-2019)
Abstract
Background: Primary sclerosing cholangitis is a rare and progressive cholestatic disease characterized by narrowing of the biliary tract. Despite high levels of ulcerative colitis, early diagnosis of primary sclerosing cholangitis before the diagnosis of inflammatory bowel disease is rarely reported.
Case history: A 28-year-old woman with no history of medical disease visited our clinic because of a mild generalized pruritus. In initial blood laboratory tests with elevated liver enzymes was observed. In Magnetic resonance cholangiopancreatography (MRCP) was introduced primary sclerosing cholangitis to the patient. Therefore, the patient was under colonoscopy and extensive colitis with terminal ileum involvement was detected and the diagnosis of ulcerative colitis was confirmed in the pathology.
Conclusion: Patients diagnosed with primary sclerosing cholangitis should undergo biopsy colonoscopy to rule out inflammatory bowel disease, even if they have no digestive tract symptoms
Fereshteh Ezzati Ghadi , Kian Aghaabbasi, Nahid Askari, Abdulla Ramzani Ghara , Masoud Torkzadeh-Mahani,
Volume 19, Issue 4 (1-2019)
Abstract
Background & objectives: Cancer is the leading cause of death worldwide. In this study, the cytotoxic effect of hydroalcoholic extract of Colutea persica leaf and its synergic effect with doxorubicin were investigated on MCF-7, LNCaP and SKM (as control) cell lines.
Methods: Hydroalcoholic leaf extract of Colutea persica was prepared using maceration method and ethanol 70%. Breast cancer (MCF7), prostate (LNCaP) and fibroblast (SKM) cell lines were cultured in microplates (96 wells) and exposed to various concentrations (10, 7.5, 5, 2.5, 1.25, 0.625, 0.312 and 0.156 mg/ml) of plant extract and doxorubicin (20, 80, 320 and 640 nM) solution. The synergistic effect of 20 nanomol of drug and 0.156 mg / ml of the plant extract was investigated. MTT assay was employed to evaluate the cytotoxic effects of the extract on cell lines at different time intervals (24, 48 and 72 hours). Staining with annexin V and propidium iodide (PI) was used to identify different types of cell death either necrosis or apoptosis.
Results: The plant extracts had cytotoxic effect and cell viability rate was lower than fibroblasts. At different times, the concentration of 10 mg /ml of the extract showed the most growth inhibition of breast and prostate cell lines. The combination effect of plant extract with doxorubicin on cells was not significant (p<0.01). The Annexin V/PI flow cytometry results showed that the percentage of initial apoptosis, delayed apoptosis and necrosis in treated cells increased compared to untreated cell.
Conclusion: Hydroalcoholic extract of Colutea persica leaf inhibits the growth of cancer cells and induce apoptosis in breast and prostate cancer cells.
Keyvan Amirshahrokhi,
Volume 19, Issue 4 (1-2019)
Abstract
Background & objectives: Paraquat is a toxic herbicide that is widely used in many countries. Paraquat selectively accumulates in the lung tissue and leads to severe pulmonary injury through the oxidative and inflammatory processes. The plant Rosa canina L. has been used for long years as an herbal medicine throughout the world. Rosa canina L. is a member of Rosacea family and shows therapeutic effects against numerous disorders by its antioxidant and anti-inflammatory properties. The aim of this study was to evaluate the antioxidant effect of Rosa canina fruits on the paraquat induced lung injury in a mouse model.
Methods: Animal experiments were performed on male NMRI mice. Paraquat was injected at a single dose (20 mg/kg, ip) to induce pulmonary damage in mice. The extract of Rosa canina fruits (200 and 400 mg/kg/day, orally) administered by gavage for four days after the injection of paraquat. The treated mice were compared to normal mice as a control group. At the end of the experiment, lung tissue samples were collected for biochemical analysis.
Results: The results showed that administration of paraquat caused an oxidant-antioxidant imbalance in the lung tissue. However, treatment with Rosa canina increased the levels of glutathione (GSH) and catalase (CAT) antioxidants and decreased the levels of malondialdehyde (MDA) and myeloperoxidase (MPO) in the lung tissues compared with those in the paraquat group.
Conclusion: Treatment of paraquat-exposed mice with the extract of Rosa canina fruits improved oxidant-antioxidant balance in the lung tissue of mice. In other words, the present study demonstrated that Rosa canina has remarkable antioxidant effects against paraquat induced oxidative damage.
Anoushirvan Sadigh, Ebrahim Fataei, Mohsen Arzanloo, Ali Akbar Imani,
Volume 19, Issue 4 (1-2019)
Abstract
Background & objectives: The purpose of this study was to determine the bacterial bioaerosols in indoor air of Ardabil universities and to investigate the factors affecting their concentration in Ardabil city.
Methods: Air sampling was performed using Andersen single-stage sampler at a flow rate of 28.3 liters per minute and a respiratory range of 10 min. In this study, trypticase soy agar containing cycloheximide antibiotic was used for bacterial culture. Biochemical tests such as DNase, catalase, oxidase, coagulase, bile esculin hydrolysis test, urease, citrate test, antibiotic resistance to novobiocin, bacitracin and optochin were used for identification and differentiation of isolates.
Result: The mean bacterial concentration in Ardabil university of medical sciences in the open air, outside the college, the halls, the classrooms and the staff rooms was 18, 88.4, 76.6, 77.4 CFU/m3 , respectively. The concentration of bacteria in Islamic Azad university of Ardabil in the open air, outside the college, the halls, the classrooms and the staff rooms was 103, 97, 124, 132 CFU/m3, respectively. The dominant species of bacteria in indoor air were Staphylococcus aureus, Staphylococcus epidermidis, Actinomycetes and Bacillus, respectively.
Conclusion: According to the results of this study, it is found that the concentration of bacterial bioaerosols in indoor air is not more than standard, but the abundance of bacterial species can cause lung, intestinal and other diseases in academic personnel, staff and students in the long-term.
Aylar Imani, Marefat Siahkouhian , Pouran Karimi, Masoud Asgharpour-Arshad , Farnaz Seifi-Ski-Shahr ,
Volume 20, Issue 1 (4-2020)
Abstract
Background & objectives: Physical training and some supplements through different mechanisms could be effective in reducing apoptosis; the aim of this study was to investigate the effect of endurance training and supplementation of Persia Rosa extract on mitochondrial apoptosis of myocardium.
Methods: In this experimental study, 52 male rats were randomly assigned into five homogeneous groups, three-month control, six months control, Persia Rosa supplement, endurance training and endurance training+Persia Rosa. Training groups participated in endurance training protocol for 12 weeks. The Persia Rosa and endurance training+Persia Rosa groups received 1 gram Persia Rosa extract per kg body weight. After the implementation of the training protocol and consumption of the extract, the surgical and cardiac tissue extraction procedures were performed. The data were analyzed by ANOVA test and Bonferroni post hoc tests at the significant level of less than 5% using SPSS-22 software.
Results: The results showed that Bax/Bcl-2 ratio and Caspase-3 gene expression were significantly decreased in endurance training and endurance training+Persia Rosa groups to the control group (p˂0.05). Bax/Bcl-2 showed a significant decrease in the Persia Rosa supplement group (p˂0.05), but Caspase-3 was not significant in the Persia Rosa supplement group.
Conclusion: It is expected that the effect of endurance training with consumption of Persia Rosa extract on Bax/Bcl-2 ratio and Caspase-3 will provide a good platform for caspase mechanisms and apoptosis processes in the myocardium of rats.
Maryam Zeraati, Belal Mosaferi,
Volume 20, Issue 4 (1-2021)
Abstract
Background & objectives: Epilepsy is one of the most common problems in the health system, of which some cases are resistant to treatment. Recently, environmental enrichment has shown beneficial results in the recovery of some cases of epilepsy.
Methods: Male mice were reared in an enriched or normal medium during their growth period. To induce seizures, at adult age, each group was divided into two subgroups, which one of them received pentylenetetrazole eleven times, with two days interval.
Results: The enriched environment greatly reduced seizure behaviors and prevented the occurrence of anxiety-like behavior and cognitive disorders. It also prevented an increase in the inflammatory cytokine of TNF-α in the hippocampus.
Conclusion: Therefore, a suitable growth environment in childhood and adolescence can be useful in preventing seizure disorders in adulthood.
Hashem Haghdopst, Mohammad Hossein Esmaeili, Mohammad Sofiabadi, Pouria Solimany, Samira Esmaeili,
Volume 21, Issue 2 (7-2021)
Abstract
Background & objectives: Alzheimer’s disease (AD) patients suffer from anxiety and depression. Sodium hydrosulfide (NaHS) can remit the depressive-like and anxiety-like behaviors induced by diabetes mellitus. We aimed to investigate the effects of chronic administration of hydrogen sulfide on depressive and anxiety-like behaviors in the Streptozotocin (STZ) rat model of AD.
Methods: Animals were divided into: Control, NaHS, and Alzheimer’s rats group include (STZ, STZ + Saline and STZ + NaHS groups) which were the Alzheimer’s rats and received Saline and NaHS (5.6 mg/kg per d) for 21 days. For induction of AD, STZ (3 mg/kg, 10 μl/injection site) was administered into the lateral ventricles. The behavioral consequences were assessed using plus maze, forced swim and sucrose preference tests.
Results: Our results showed that intracerebroventricular (i.c.v.) injection of STZ decreased the percentage of open arm time and entries, indicating anxiety-like effects. It also increased the duration of immobility time and decreased the percentage of sucrose preference indicating depression-like effects. Sodium hydrosulfide administration in STZ-treated rats increased the percentage of open arm time and entries, indicating anxiolytic-like effects. It also decreased the duration of immobility time and increased the percentage of sucrose preference, indicating antidepressant-like effects.
Conclusion: STZ administration can induce depression- and anxiety-like symptoms in rats, and Sodium hydrosulfide treatment, decreased the depression- and anxiety-like symptoms in STZ rat Model of AD, suggests that Sodium hydrosulfide can be useful in the treatment of affective disorders in AD patients.
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