|
|
|
 |
Search published articles |
 |
|
Showing 30 results for Dat
Reza Alipanahmogadam , Mohammad Mazani , Abbas Naghizadeh Baghi, Ali Nemati, Mojtaba Amani , Bahman Bashardoost, Amir Mansour Vatankhah , Shahab Bohlooli,
Volume 11, Issue 3 (9-2011)
Abstract
Background & Objectives: Several evidences show that oxidative stress is high among patients with hemodialysis . This study was aimed to investigate the oxidative stress and serum level of zinc and copper in patients with non-diabetic hemodialysis . Methods: In a case-control study, 60 patients with non-diabetic hemdialysis referred to hemodialysis section, Ardabil Bou-Ali hospital, and 60 healthy individuals, matched by age and sex, were selected. In each group, lipid profile, blood glucose, creatinine, urea, total protein, albumin, zinc, copper, malondialdehyde, total antioxidant capacity, activity of glutathione peroxidase and superoxide dismutase were measured. The results were analyzed statistically by using Pearson correlation coefficient and Student-t test. Results: The amount of zinc, blood sugar, cholesterol, triglyceride, total protein, albumin, high density lipoprotein-C and low density lipoprotein-C were significantly lower in patients with hemodialysis in comparison with those in normal individuals. T he activity of superoxide dismutase, glutathione peroxidase in red blood cells and serum total antioxidant capacity were significantly high in patients in comparison with those in control group. However, serum concentrations of copper and malondialdehyde in two groups were not statistically different. Conclusion : The results of this study show that increase of antioxidant enzymes activity reduces the oxidative stress status in patients with non-diabetic hemodialysis . Zinc deficiency was also observed in these patients.
Eiraj Feyzi, Amin Bagheri , Samira Matin ,
Volume 11, Issue 4 (12-2011)
Abstract
Hydatid disease infection in humans is caused by larva of Echinococcus granulosus. Definitive hosts are carnivores such as dogs, while intermediate hosts are herbivores. Humans can also be intermediate hosts. Hydatid disease is clinically related to the presence of cysts, most frequently in the liver and the lungs and less frequently in the other organs such as kidney, spleen, brain, heart and bone. In this article, a case of splenic hydatid cyst is reported. A 50-years-old man was admitted to the clinic with a abdominal pain lasting for two weeks. Sonography and computed tomography (CT) scan revealed a splenic cyst. Cyst was removed by a surgery. The diagnosis of a hydatid cyst was confirmed by histopathology.
Adel Spotin, Monireh Mokhtari Amirmajdi , Mojtaba Sankian, Abdolreza Varasteh, Ali Akbar Shamsian, Fatemeh Vahedi ,
Volume 12, Issue 1 (4-2012)
Abstract
Background & objectives: Hydaticosis is a zoonotic helminthic disease of human and other intermediated hosts in which larval stages of the tapeworm Echinococcus granulosu transfect human. The liver and lung are the host tissues for the hydatid cyst . It is unknown which mechanisms are involved in infertility of the cyst and suppression of the fertile cyst. This study was aimed to evaluate the expression of the apoptosis inducing-ligands such as TRAIL and Fas-L in germinal layer of the cyst and human normal tissue surrounding the cyst that is one of the unknown host innate immunity mechanisms against the hydatid cyst. Methods: In this study, four isolated hydatid cysts were used which had been diagnosed in patients by radiography and parasitological examination in Mashhad Ghaem hospital. Furthermore, the germinal layer of the cyst and accompanied normal peripheral tissues were separated by scalpel in sterile conditions. After homogenization, expression of TRAIL and Fas-L genes were studied by semi-quantitive RT-PCR method. Results: The TRAIL and Fas-L showed significant higher level expression in germinal layer of infertile cyst than the fertile cyst and host normal tissues. Conclusion: The host tissue-induced apoptosis of germinal layer of the fertile cysts is probably one of the infertility mechanism in patients with hydaticosis
Sara Rahbar, Naser Ahmadiasl,
Volume 12, Issue 3 (9-2012)
Abstract
Background & Objectives: Numerous studies have been conducted to assess the effects of acute resistance exercises on the structure and the function of heart, but little works done on effects of chronic resistance exercises. So, the objective of current study was to investigate the long term effect of regular exercises on cardiac function and oxidative stress. Methods: Forty male Wistar rats in the weight range of 250- 300 g were used in this study. They were divided in 2 following groups: The 3 months exercises test group and control group which remained without exercises. Regular resistive exercise was carried out according to the model proposed by Tamaki et al. Test group rats exercised for three months. Finally the hearts of 10 rats in each group were taken for homogenization, oxidative stress measurement and the other ten were examined for heart function. Malondialdhyde as an index of oxidative stress and superoxide dismutase, glutathione peroxides and catalase as an indicator of antioxidant capacity with special kits were specifically measured. Results: Regular resistive exercise didn't significantly affect the rats' weight, but heart weight in exercise group showed a significant increase (p<0.05). There was a significant decrease in heart rate in exercise group (p<0.05). Left ventricle contraction strength and coronary flow had a significant increase in exercise group in comparison with control group (p<0.05). There was not any significant difference in Malondialdhyde and antioxidant enzymes activity. Conclusion: This study showed that, heart efficiency had a significant improvement under effect of regular resistive exercise. Meanwhile, regular resistive exercise didn’t have any significant effect on oxidative stress and heart antioxidant defense capacity.
Manoochehr Iranparvar Alamdari, Hosein Ghorbani Behrooz, Abbas Yazdanbood, Naiyereh Amini Sani , Solmaz Islam Panah, Mahmood Shokrabadi,
Volume 12, Issue 3 (9-2012)
Abstract
Background & Objectives: Diabetes Mellitus is a metabolic disorder in which lacking of control and suitable care lead to disability and mortality. American Diabetes Association (ADA) has emphasized the medical care of diabetic patients and has suggested several objectives to increase survival and improve health outcomes with low complications by controlling the glycemic, lipids, nerupathy and hypertension as well as foot care, nutritional therapy and screening of cardiovascular disease. The aim of this study was to evaluate the quality of care in diabetic patients from Ardabil and its concordance to the standards recommended by ADA. Methods: In a cross sectional study, 100 diabetic patients referring to a clinic of diabetes in Ardabil (2005) were randomly selected and enrolled. The study was conducted according to a questionnaire with 90 questions and physical examination and Final lab tests. Data were collected and analyzed with Spss version 12. ANOVA test was used to compare groups. Results: The 12 months recorded lab tests for diabetic patients were as follows: Hb A1C in 33%, MicroAlbuminuria in 16%, HDL in 58% and LDL in 55% of patients had been checked. Foot and peripheral pulses exams were only recorded in 9 and 5% of patients respectively. In final lab tests, 24% of patients had favorable FBS level (90- 130mg/dl). 46% of patients had suitable Hb A1C and 32% patients had MicroAlbuminuria. Aspirin was recommended to 45% of patients over 50 years old. Only 10% of patient for three lab tests (FBS, LDL cholesterol, Hb A1C) achieved the intended aims. According to ADA recommendations 22% of patients for three risk factors (BP, LDL cholesterol & Total cholesterol) were in optimal level. Conclusion: There is a high difference between current diabetes care in our clinic and ADA goals.
Ali Shadmanfard, Ali Nemati , Abbas Naghizadeh Baghi, Mohammad Mazani,
Volume 12, Issue 5 (11-2012)
Abstract
Background & Objectives: The intake of antioxidant supplementations can have key role in prevention of oxidative stress in healthy individuals. Pomegranate has antioxidant effects and may play a role in reduction of oxidative stress in healthy males. Therefore, this study examined the effect of pomegranate juice supplementation on oxidative stress in young healthy males. Methods : In semi-experimental study, 14 healthy students living in Dormitories of Ardabil University of Medical Sciences were included. Subjects were given one cup of pomegranate juice supplementation per day for two weeks . Fasting blood samples were taken both at the start and the end of 2-week period to measure the antioxidant enzyme activities such as superoxide dismutase , glutathione peroxidase , paraoxonase - 1, aryl esterase , and the values of serum total antioxidant capacity , glutathione, and lipid profiles . Data were analyzed using descriptive and paired t - test s. Results: T he level of serum total antioxidant capacity and activities of enzymes such as superoxide dismutase, glutathione peroxidase, arylesterase, and standardized arylesterase activity were significantly increased at the end of two weeks (p<0.05). The serum level of malondialdehyde was significantly decreased after intervention (p<0.05). Changes were not significant, although the serum levels of glutathione and HDL- cholesterol increased and LDL- cholesterol decreased at the end of two weeks period. Conclusion: The results of this study showed that the pomegranate juice supplementation had beneficial effects in helping body's antioxidant defense system and reduction of oxidative stress in young healthy males . This study suggests that the pomegranate juice supplementation can be useful against oxidative stress included in dietaries of young healthy males.
Mostafa Nachvak , Sheida Ahani Kamangar , Ali Nemati , Saeid Sadeghieh Ahari ,
Volume 14, Issue 2 (7-2014)
Abstract
Background & Objectives: Epidemiological and experimental evidences suggest that oxidative stress is elevated in children with Down syndrome (DS). Some studies show that consumption of diets with a high content of fruits and vegetables results in a significant reduction in markers of oxidative stress. We investigated the frequency of fruits and vegetables intake in children with DS and the influence of variations in the level of this consumption on biomarkers of oxidative stress . Methods: Frequency of consumption of fruit and vegetables by children with DS were recorded by interview with the children’s parents. Serum malondialdehyde (MDA) and urinary 8-hydroxy-2'-deoxyguanosine (8-OHdG) were also measured as biomarkers of oxidative stress. Data analysed using ANOVA and Student’s t-tests . Results : Mean consumption of fruits and vegetables was 4.6 and 0.74 servings per week, respectively. No significant relationships were found between consumption of fruits and vegetables with markers of oxidative stress. Based on our findings, the 65.9 % of the children did not eat any vegetables and the rest of the children consumed less than one serving per day. There was a significant correlation between the frequency of fruits and vegetable consumption (r=0.212 p <0.04). Conclusion : There wasn’t any significant relationship between fruits and vegetables consumption and indicators of oxidative stress in children with DS. This lack of correlation could be due to low consumption of fruits and vegetables in children with DS.
Amirabbas Ghasemi , Behnam Daneshpajouh ,
Volume 14, Issue 3 (10-2014)
Abstract
Background &objectives: Echinococcosis, referred to as hydatid disease or hydatidosis, is a parasitic disease caused by larval stage of Echinococcus granulosus. In the life cycle of this parasite, dogs and other carnivores are definitive hosts and herbivores are intermediate hosts. Humansin this cycle may incidentally involve by ingestion of parasite eggs and play as intermediate host. Hydatid disease can involve any organ but liver and lung are the most commonly affected organs and kidney, brain, heart, bone may also be affected to a lesser degrees. Cerebral hydatid cyst occurs in about 2% of all echinococcosis cases. In this paper we report two cases of cerebral echinococcosis in whom diagnoses were made based on MRI findings and confirmed by pathology reports after surgery. First Case Report: The first case was a 30 years old man who was admitted after an episode of generalized tonic-clonicseizure. The MRI imaging performedand patient underwent surgery with diagnosis of a right frontal cystic lesion. The patient was improved progressively and there was no seizure attack and recurrence during 18 months follow up period. Second Case Report: The second case was a 28 years old woman presented by headache and abnormal behavior. MRI of this patient revealed a large cystic lesion in left frontal lobe and then underwent surgery. No complication was seen during postoperative period. The headache and behavioral disorder were improved completely and there was no sign of recurrence during 18 months follow up period. Conclusion: Hydatid cyst should be considered as a differential diagnosis of cystic brain lesions in endemic areas. Although hydatid cyst is a benign lesion but surgical removal of it without rupture is very important for prevention of probable future complications.
Farnaz Seifi-Skishahr, Arsalan Damirchi , Manoochehr Farjaminezhad , Parvin Babaei ,
Volume 15, Issue 1 (4-2015)
Abstract
Background & objectives: The “redox” state represents the oxidation/reduction potential within the cell in a way that more “redox” is the marker of health, while the more oxidized reflects predisposition to diseases. Different types of exercise training may change the thiol/disulfide ratio of redox couples such as glutathione and represent a shift in redox balance. This study was assessed the influence of high-intensity aerobic exercise on glutathione redox state in red blood cells in professional, recreational athletes and nonathletes.
Methods: Ten voluntary well trained (WT), moderately trained (MT) and untrained men subjectswere randomly selected for this semi-experimental study (mean ages of 21.10±1.72 21.70±1.88 and 20.10±1.44, respectively). Blood samples were collected before, immediately, 10 min and 30 min after acute aerobic exercise with 75%VO2max. The levels of reduced glutathione (GSH), oxidized glutathione (GSSG) and (GSH/GSSG) in red blood cells (RBCs) as well as serum levels of cortisol and creatine kinase (CK) were measured.
Results: The results showed reduction, elevation and no changes in RBCs GSH/GSSG ratio in UT, MT and WT groups, respectively. The lowest levels of GSH/GSSG ratio in RBCs and the highest one were detected in the WT and MT groups, respectively. The serum levels of cortisol and creatine kinase were increased following the exercise in three groups.
Conclusion: It is concluded that acute aerobic exercise with high intensity does not change redox balance in well trained subjects, however it is capable to shift redox balance towards more reducing environment in moderately trained subjects and also to more oxidizing one in untrained subjects.
K Tahmasebi, M Jafari, F Izadi,
Volume 15, Issue 2 (7-2015)
Abstract
Background & objectives: Diazinon (DZN) as an organophosphate pesticide widely used in agriculture is associated with reducing the antioxidant capacity of the cell. Use of thiol compounds such as N-acetyl cysteine (NAC) as an antioxidant decreases oxidative stress in the cells. The aim of this study was to investigate the effect of NAC as an antioxidant against DZN- induced oxidative stress in rat brain and heart.
Methods: In present experimental study, male Wistar rats were randomly divided into four groups including: control group (corn oil as DZN solvent), DZN group (100 mg/kg), NAC group (160 mg/kg), and NAC+DZN, all of which were given intraperitoneally. 24 hours after injection, animals were anesthetized by ether, and the brain and heart tissues were quickly removed. After tissues hemogenation, superoxide dismutase (SOD), catalase (CAT), glutathione S-transferase (GST) and lactate dehydrogenase (LDH) activities, as well as glutathione (GSH) and malondialdehyde (MDA) levels were determined by biochemical methods.
Results: DZN increased SOD and GST activities and MDA level and decreased LDH activity and GSH content in brain and heart. Also, DZN increased CAT activity in the heart and increased it in the brain. Administration of NAC inhibited the change in these parameters.
Conclusion: DZN through free radical production leads to enhanced membrane lipid peroxidation, depleted GSH content and oxidative stress induction in the brain and the heart. Administration of NAC as antioxidant decreases the DZN-induced oxidative stress by scavenging free radicals and GSH synthesis, but its protection is not complete.
Somaye Bahrami , Mohammad Hossein Razi Jalali , Zahra Ramezani, Mehdi Pourmehdi Boroujeni , Ferial Toeimepour ,
Volume 15, Issue 4 (1-2015)
Abstract
Background & objectives: One of the most important zoonotic parasitic diseases, hydatidosis, is caused by the larval stage of Echinococcous granulosus. Investigations have shown that plants secondary metabolites, such as essential oils have anti parasitic properties. Based on previous reports on antiparasitic properties of Lepidium sativum, in this study we investigated the scolicidal effects of the essential oil (EO) extracted from this plant.
Methods: Lepidium EO was obtained by hydrodistillation method. Gas chromatography-mass spectrometry (GC-MS) was employed to determine the chemical composition of the EO. Protoscolices were exposed to various concentrations of EO (1, 3, 5, 10 and 15 mg/ml) for 10, 20, 30 and 60 min. Viability of protoscolices was confirmed by 0.1% eosin staining.
Results: A total of 19 compounds representing 95.5% of the total oil, were identified. α-Thujene (88.86%), Myrcene (2.9%) and P-cymene (1.67%) were found to be the major EO constituents. Based on the results, protoscolices mortality rates at 1, 3 and 5 mg/ml of EO didn’t have a significant relationship with the control group. While, the difference in mortality rate at a concentration of 10 mg/ml of EO in 30 and 60 min was significant. Also, the concentration of 15 mg/ml of EO at all times of incubation had significantly higher protoscolicidal effect. In the present study there was a significant relation between the amount of protoscolicidal activity of different EO concentrations and different incubation times. In other words mortality rates enhanced with increasing concentrations and incubation times.
Conclusion: The results of present study revealed that the EO of Lepidium is rich in α-Thujene and has a high scolicidal power. This plant may be used as a natural scolicidal agent
Somayeh Bahrami, Hossein Najafzadeh, Ali Shahriari, Sara Ahmadi, Mohammadhossein Razi Jalali ,
Volume 16, Issue 4 (1-2016)
Abstract
Background & objectives: Polyamines such as putrescine, spermidine, and spermine are ubiquitous in all eukaryotic cells and play an essential role in cell division and differentiation. One way of polyamine biosynthesis is done by ornithine decarboxylase (ODC) which catalyzes the transformation of ornithine to putrescine. The aim of the present study was to evaluate the level of putrescine, spermidine and spermine in protoscolices, hydatid fluid and germinal layer and also to evaluate ODC activity.
Methods: In the present study putrescine, spermidine and spermine levels were investigated in germinal layers, hydatid fluids and protoscolices. To evaluate the activity of ODC, protoscolices were incubated with ornithine and changes in polyamines level were assayed. The samples were homogenized and liquid chromatography (HPLC) was used for polyamines measurement.
Results: Based on the results, putrescine was the lowest polyamine and since its level was not increased in protoscolices incubated with ornithine, ODC activity was not detected. Spermidine was the highest polyamine and the results showed that germinal layer contained the highest level of polyamines.
Conclusion: Overall, the results showed that ODC activity was not detected in hydatid cyst and level of polyamines in germinal layers which contained rapidly proliferating cells was higher than other parts.
Bahador Karimi, Zohreh Ghotbeddin, Seyed Reza Fatemi Tabatabaei ,
Volume 16, Issue 4 (1-2016)
Abstract
Background & objectives: Zinc as one of the most important trace elements is needed for proper functioning of the nervous system and homeostasis. Many studies show that stress causes memory impairment through various mechanisms, including oxidative stress induction and some mechanisms which are directly effecting brain function. So, in this work we assessed the effect of zinc chloride on passive avoidance memory and oxidative stress following acute stress in male rats.
Methods: In this study, 50 male Wistar rats were used in five groups: control, sham, stress, zinc chloride treatment and zinc chloride treatment before stress induction. For stress induction, rats were restrained (not immobilized) for 6 h/day, 7 days in a Plexiglas restrainer, and treated rats received an oral dose of zinc chloride 32 mg/kg/day by gavage for 6 days. At the end of the experiment, passive avoidance memory was avaluated by shuttle box and some oxidative damage markers were determined in all groups.
Results: Results of this study showed that animals which were exposed to stress showed a significant decrease in passive avoidance memory compared to control group (p<0.01) and the oxidative stress parameters in this group showed significant changes compared to the control group (p<0.05). While passive avoidance memory and oxidative stress parameters in group treated with zinc chloride were nearly closed to control group.
Conclusion: According to our results, zinc chloride with antioxidant properties can have a protective effect on memory impairment and oxidative stress induced by stress.
Taktam Sadat Vafa , Mojdeh Emadi , Seyed Damoon Sadoughi,
Volume 18, Issue 1 (4-2018)
Abstract
Background & objectives: Curcumin has antioxidant properties. The aim of this study was to determine the effect of curcumin on bax, bcl-2, antioxidant enzymes and lipid peroxidation of sperm after freezing procedure.
Methods: In this experimental study, semen samples were collected from four mature Holstein bulls, twice a week in eight innings. Semen samples were divided into four groups. Zero (control), 10 (Experimental group one), 20 (Experimental group two) and 30 (Experimental group three) mg/ml of curcumin with diluents were added to the semen samples. After thawing, Bax, Bcl-2 and malondialdehyde levels as well as superoxide dismutase, glutathione peroxidase and catalase enzymes were measured in sperm samples using ELISA.
Results: According to the results, Bcl-2, superoxide dismutase, glutathione peroxidase and catalase levels in sperm samples treated with 20 and 30 mg/ml curcumin significantly increased and Bax and malondialdehyde levels significantly decreased compared to control groups (p<0.05). This difference was not significant for sperm samples treated with 10 mg/ml curcumin.
Conclusion: Dose-dependent administration of curcumin decreased oxidative stress and lipid peroxidation and increased anti-apoptosis proteins in freeze-thawing sperms.
Keyvan Amirshahrokhi , Shahab Bohlooli , Mohammad Yousefi ,
Volume 18, Issue 3 (10-2018)
Abstract
Background & objectives: The purpose of this study was to show the dose response relationship of anti-inflammatory effect of methylsulfonylmethane (MSM) on carrageenan induced rat paw edema as an acute model of inflammation.
Methods: A total of 54 male, Sprague-Dawley rats, weighing 180-190 g, were used. One hundred, 200, 400, 800 and 1200 mg/kg of MSM were administered intraperitoneally to the rats 30 minutes before induction of paw edema with injection of 0.1% carrageenan. Diclofenac was used as a control drug. Rats were divided into three groups: MSM, diclofenac and normal saline, and their paw tissue were collected for the study of inflammatory and oxidative markers (MDA, GSH, TNF-α and IL-1 β). The relationship between the different concentrations of MSM and decrease in rat paw edema was calculated using a simple Emax model.
Results: the ED50 value for effect of MSM on carrageenan induced rat paw edema was 193±9.7 mg/kg. A significant reduction in paw edema following administration of MSM at 200, 400, 800 and 1200 mg/kg was observed, but statistical analysis did not reveal any significant reduction in paw edema after administration of 100 mg/kg. MSM did not show statistically significant difference from control group in tissue level of GSH, but it was able to decrease MDA level significantly. MSM was able to significantly alleviate IL-1 β and TNF-alpha tissue levels.
Conclusion: The recommended anti-inflammatory dosing range of MSM is 200-800 mg/kg for pharmacological studies in rats and the average appropriate dose is 400 mg/kg. Also, it seems that anti-inflammatory effect of MSM is more profound than its anti-oxidant effects.
Iraj Feizi, Somaieh Matin, Hasan Ghobadi, Saeed Hosseininia, Susan Mohammadi Kebar, Farideh Feizi, Hossein Salehzade,
Volume 19, Issue 3 (10-2019)
Abstract
Background & objectives: Hydatid cyst is a major hygiene problem in Iran. In 80% of cases, only one organ is involved. However, multiorgan involvement has also been rarely reported. But it seems, the prevalence of hydatid cyst with the involvement of more than one organ in Iran is more than other world. The aim of this study was to evaluate the patients with hydatid cyst multi organ involvement in Ardabil province in northwest of Iran.
Methods: In this retrospective study, 34 patients with hydatid cyst with involvement in more than one organ were included. For each patient, a pre-designed questionnaire including name, age, education level, occupation, place of residence, cyst location, clinical manifestations and serologic test results were completed. Patients, who had previously a history of treatment due to hydatid cyst, were excluded from the study.
Results: The mean age of patients was 36.41 years. 61.8% of the patients were housewives and 64% were rural residents. The most commonly involved organs was liver and simultaneous involvement of liver and lung was observed in 38.2% of patients and was more in women. The association of the right lung cyst with the liver cysts was greater than that of the left lung. Only in 43% of the patients, the serologic test was positive for hydatid cyst, and the relationship between serologic test and type of involvement was not significant.
Conclusion: Regarding the high incidence of hydatid cyst involvement in more than one location in our region, in all patients, especially women with liver hydatid cyst, other organs, especially the lungs, should be investigated
Mehdi Valizadeh , Esmaeil Babaei, Rasoul Sharifi, Abbas Yazdanbod,
Volume 20, Issue 2 (7-2020)
Abstract
Background & objectives: Colon cancer is a common disease in the world that causes high mortality in affected people. The lack of appropriate diagnostic and prognostic markers has led to the failure in early diagnosis of colorectal malignancies. MicroRNAs play an important role in controlling the expression of target genes involved in the development and progression of colon cancer. The aim of the present study was the bioinformatics identification of microRNAs with distinct expression in cancerous and non-cancerous colon samples.
Methods: This type of study was theoretical bioinformatics and microarray data of 1513 colon cancer samples with the accession number of GSE115513 were obtained from the GEO site and marker genes were selected by using R program. Target genes of the identified microRNAs were provided by TARGETSCAN software and finally, the graphical network was plotted in Cytoscape software.
Results: Analysis of microarray data showed that has-miR-663b, has-miR-650, has-miR-17-5p, has-miR-4539 and has-miR-501-3p have biomarker potential in cancer samples. Statistical analysis and investigation of the target genes indicated that miR-663b (ROCAUC=0.8965, p=0.001) and has-miR-650 (ROCAUC=0.9104, p=0.001) had significant distinct expression between cancerous and non-tumor margins with biomarker potential.
Conclusion: The has-miR-663b and has-miR-650 genes can be used as diagnostic markers to distinguish colon cancer from non-cancerous samples
Farnaz Seyfi Askshahr, Mojdeh Khajehlandi,
Volume 20, Issue 3 (10-2020)
Abstract
Background & Objectives: Based on the probably positive role of exercise on total oxidative status (TOS) and total antioxidant system (TAS) and their effect on the process of angiogenesis, the aim of the current study was to evaluate the effect of one course of moderate-intensity endurance training on the gene expression level of vascular endothelial growth factor-B (VEGF-B) and angiopoietin-1(ANGPT-1) and TAS and TOS status in cardiac tissue of male rats.
Methods: In this study, 20 male Wistar rats were divided into two equal groups as follows: 1-training group and 2- control group: no sports activities were performed on them. Rats in the training group performed moderate endurance training for 6 weeks and 5 sessions per week from the twelfth week of life. Twenty-four hours after the last training session, heart tissue samples were extracted to measure gene expression levels of VEGF-B and ANGPT-1 and the TAS and TOS status in heart tissues. T-test with the statistical level of (p˂0.05) was used for between groups comparison.
Results: Findings showed that after six weeks of endurance training, the expression level of ANGPT-1 and VEGF-B and the amount of TAS in the exercise group significantly increased (p=0.001) and the amount of TOS significantly decreased compared to the control group (p=0.008).
Conclusion: According to the results of the present study, it seems that moderate-intensity endurance training can be effective in preventing cardiovascular disease by increasing factors involving in angiogenesis, improving TAS and reducing TOS in heart tissue of rats.
Mohammadreza Nasirzadeh, Seyedsajjad Hejazi, Mohammad Bakhshi, Saeed Taginasab, Heidar Tayefesattari, Pouya Navidi Far,
Volume 20, Issue 4 (1-2021)
Abstract
Background & objectives: Vincristine (VIN) is a broad-spectrum anticancer drug has been used to treat various cancers. Resveratrol (Res) is a natural polyphenol found in many plant sources. Many studies have reported anti-inflammatory and antioxidative effects of resveratrol. We have explored the protective effect of resveratrol on vincristine-induced oxidative stress in mouse ovarian tissue.
Methods: In this study, 32 female mice weighing 25-30 grams were randomly divided into four groups (each group n=8): 1- control group, 2- Vin- group, 3- Vin-Res group and 4- Res group. The mice received a single IP injection of vincristine (3 mg/kg) weekly for 4 weeks. Res treatment was done 28 days by gastric gavage (daily 30 mg/kg).At the end of the study, Malondialdehyde (MDA) and total antioxidant capacity (TAC) and the antioxidant activity of superoxide dismutase (SOD) and glutathione peroxidase (GPx) enzymes were measured in ovarian tissue and uterus of the studied animals. Also, ovarian follicles were counted.
Results: The results indicated that the MDA level was elevated and TAC, GPx as well as SOD activities were decreased in Vin- group significantly. Resveratrol reduced MDA level and increased GPx and SOD activities in Vin-Res group significantly. Also histological findings showed that Res increased primordial and primary follicles and reduced atretic follicles in Vin-Res group significantly.
Conclusions: These results indicate the protective effect of resveratrol on ovarian and uterine tissue against oxidative damage of vincristine in mice
Parisa Habibi, Hadi Yousefi, Mehdi Khazaei, Mohammad Zarei, Iraj Salehi, Reza Jamali Delfan, Simin Afshar,
Volume 22, Issue 2 (7-2022)
Abstract
Background & objectives: Menopause and especially acute menopause due to surgery is associated with many complications in women. The aim of this study was to determine the effects of genistein and regular swimming exercise (alone/or in combination) on pain through a possible mechanism of inflammation and oxidative stress in ovariectomized rats.
Methods: In this study, rats were divided into six groups, including: control, sham, ovariectomy (OVX), ovariectomized with eight weeks of swimming exercise training (OVX.E), ovariectomized with eight weeks of genistein administration (OVX.G), and ovariectomized with eight weeks of combined treatment (OVX.G.E). The effects of genistein and/or exercise were evaluated by examining the pain intensity with tail-flick and formalin tests. The serum levels of tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), total antioxidant capacity (TAC), total oxidant status (TOS), and malondialdehyde (MDA) were also evaluated by ELISA and spectrophotometer.
Results: In the OVX group compared to the control group, tail-flick and formalin tests showed an increase in pain response. Also, a significant increase in the serum levels of IL-1β, TNF-α, MDA, TOS and a decrease in TAC was observed in the OVX group, however, in the OVX.E, OVX.G and especially OVX.E.G groups, pro-inflammatory cytokines and oxidative stress as well as pain responses showed a significant decrease compared to the OVX group.
Conclusion: A combination of genistein and regular swimming exercise was synergistically more effective in reducing acute and chronic pain than using them alone in the postmenopausal period.
|
|
.png)
.png)
