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Showing 26 results for Ace
Nasrin Homayounfar , Fahimeh Sehhati , Afrooz Mardi , Firooz Amani , Hamid Jafarzadeh ,
Volume 7, Issue 4 (12-2007)
Abstract
Background & Objective: Depot-medroxyprogesterone acetate (DMPA) is an injectable contraceptive method that is widely used by 68 million women in over 114 countries and was reported Different medical and non medical reasons were reported for discontinuation of DMPA use. Due to little information about continuation rate and reasons for discontinuations of DMPA in this region and because of the different result of the research in other countries this study was designed. to evaluate the use of DMPAincluding continuation rate and side effects. Methods: In this cross sectional retrospective study 396 DMPA user women referring to health care centers in Ardabil were selected through classified sampling. The data were collected by a questionnaire by interviewing the subjects and data were analyzed by SPSS software using analysis variance (ANOVA) and correlation and T-test. Results: The mean age of the study population was 32±7.8 years, 33.8% had primary education and the majority of the users were housewives (%95) and only20 (%5) were employed. 6, 9, 12 and 24 month cumulative continuation rate of DMPA were recorded as %44.5, %26.5, %18.2 and %2.3 respectively. The most common reason for discontinuation among all of groups was amenorrhea. The most common side effects were amenorrhea (%69.7), low back pain (%13.4), and weight gain (%13.4) and headache (%10.4). There was a significant corvelation belween education level and spouse's education level, spouse's job, family income, grariding, number of off spring, type of previous delivery and continuity of DMPA (p<0.05). Conclusion: The result of this study could help health authorities and care givers in term of correct counseling and follow up. This factor could result in more satisfaction and improve continuation rate of DMPA.
Ghiti Rahemi , Farideh Mostafazadeh, Mehrnaz Mashofi ,
Volume 8, Issue 2 (6-2008)
Abstract
Background & Objective: As cancer of cervix is one of the important facts, for women and probably causes in presence, furthermore early diagnosis and management in primary stages is very effective for prognosis of the disease and survival, the aim of the study was to measure the probable relationship of contraraceptive devices with cervical lesions. Methods: This study was a descriptive analytic study that was done by referring to all pathologic center (6 centers) in Ardabil and reviewing 399 reports of pap smear through sampling method (one of them because of chemotherapy was excluded from the samples) Results: In this study 90 persons 22.5% of pop smear’s study were normal and 309 persons 77.5% were abnormal that include nonspecific cervicitis (298 persons) specific cervicitis (192 persons) and epithelial cell abnormality (105 persons) Regarding the use of contraceptive devices, 15 persons didn't use any device 3.8% And 172 persons 43% were using one of the natural methods or tubal ligation, and 131 persons were using OCP pills 32.9% 63 persons were using IUD 15.8% 18 persons 4.5% were using condom, and also DMPA was not used. Through chi test it was found that there was significant relationship between contraceptive methods and epithelial cell abnormality (p=0.008) and imperavite cervicitis (p=0.001). There was a meaningful relationship. Conclusion: There was a meaningful relationship between using contraceptive methods and nonspecific cervicitis.
Masoumeh Kazemi, Hedayat Sahraie , Mahnaz Azarnia , Hosein Bahadoran,
Volume 10, Issue 2 (6-2010)
Abstract
Background & Objectives: Previous studies indicated that morphine consumption during pregnancy could inhibit embryos development. Present study further evaluated the effects of oral morphine consumption on the maternal and fetal portion placenta cells development in Wistar rats. Methods: Female Wistar rats (W: 170-200 g) were used in the present study. Morphine group were received morphine (0.05 mg/ml of tap water) after one night coupling with male rats for mating. On 14th, 17th days of pregnancy, the pregnant animals were killed with chloroform and the placentas and uterus were removed surgically and fixed in 10 % formalin. The fixed placentas and uterus were stained by H & E method and evaluated for their development. The thickness of layers, as well as number of the cells in both maternal and fetal parts of the placentas was determined by light microscopy and processed using MOTIC software. Results: The results indicated that oral consumption of morphine compared to control group, increased the thickness of the layers in maternal portion and also, increased the number of the cells in both maternal and fetal portion of the placenta. Conclusion: All together, oral morphine consumption may inhibit placenta cells development and disturb their natural functions. These abnormalities observed in the placenta by opioid addicted pregnancy Wistar rats.
Gholamreza Sepehri , Vahid Sheibani , Yasamin Pahlavan , Mohammadreza Afarinesh Khaki , Khadijeh Esmail Pour Bezenjani, Bahareh Pahlavan ,
Volume 11, Issue 1 (4-2011)
Abstract
Background & objectives: Origanum Vulgare is an herbal plant which is widely distributed in the north and northwest parts of Iran and posses therapeutic properties. The aim of this study was to evaluate the effect of intracerebroventricular (ICV) injection of aqueous extract of Origanum Vulgare L. ssp. viride on pain threshold in male rats. Methods: In this study,28 Male Wistar rats (200-250 g) were divided randomly into 4 groups (n=7) . Rats were anaesthetized with a mixture of ketamine and xylazine and placed in stereotaxic apparatus. A guide cannula was inserted into ventricular area, according to the atlas of Paxinos and Watson. One week after surgery , three groups of rats received microinjection of Origanum extract (1, 3, 6µg/rat). Controls received the microinjection of the same volume of saline. Tail flick latency (TFL) was used to assess the nociceptive response each 15 min for 120 min, using tail flick test. The results were analyzed by repeated measurement test. One-way analysis of variance (ANOVA) was used to compare the mean values of quantitative variable among the groups. The data are expressed as mean ± SEM. p < 0.05 was considered significant. Results: Intracerebroventricular administration of the Origanum extract resulted in significant and dose-dependent increase in TFL compared to controls (p<0.05). Origanum extract at dose of 3µg/rat showed the highest analgesic response. The maximum analgesic response was observed at 60 min and 90 min post extract injection (p<0.05). Conclusion: These results suggest that the ICV injection of aqueous extract of Origanum Vulgare possesses dose- dependent antinociceptive activities in the tail flick test in rats.
Afsaneh Vaisy , Shirin Lotfinejad , Faiegh Zhian,
Volume 13, Issue 3 (10-2013)
Abstract
Background & Objectives: Cervical cancer is the third common cancer among women in United States and in developing countries cervical cancer is supposed as the first or second common cancer of female reproductive system. The aim of this study was to determine the relationship between cervical cancer and its risk factors in Iranian women. Methods: This is an observational, retrospective study. A total of 128 patients with confirmed diagnosis of cervical cancer were compared with the control group of 128 healthy women. The risk factors such as age, age of the first pregnancy, abortion, termination of pregnancy, number of pregnancy and the socioeconomic status were compared and analyzed by Student's t-distribution test, Fisher’s and Pearson’s statistical methods. Results: The following odds ratio can be concluded from the study: Marital status 2.71, having multiple sexual partners 3.33, marriage under the age of 16 1.61, use of oral contraceptives 3.072 and using cauterization and cryotherapy were 0.6. Conclusion: This study showed that marital status, having multiple sexual partners, low marital age and history of using oral contraceptive consumption increase the possibility of cervical cancer.
Fereshteh Sharifi Ferdoey , Shiva Irani, Mojghan Zandi, Masoud Soleimani,
Volume 14, Issue 3 (10-2014)
Abstract
Background & objectives : T he main goal of tissue engineering is regeneration and restoration of damaged tissues and organs, besides being used in medicine . Scaffolds are the main segments for tissue engineering, and plasma surface modification is one of the modern methods used for surface modification on polymer scaffolds. The aim of this study was to evaluate the effect of nano-fibers with different densities on fibroblasts' behavior besides the plasma surface modification. Methods : Poly Ɛ-Caprolactone nano-fibers (PCL) were developed by an electro-spinning technique at different collecting times. These nano-fibers were then modified by oxygen plasma. Cellular attachment to the nano-fiber and their morphology were evaluated using scanning electron microscope (SEM) and cellular activities were also studied by 3-[4,5-dimethylthiazol- 2-yl]-2,5 diphenyltetrazolium bromide (MTT). Scaffold biocompatibility test was assessed using inverted microscope. Results : Scanning electron microscope images of nano-fibers showed that increase in time of spinning has significantly heighten fiber density, on the other hand plasma surface modification of nano-fibers had significant effects on their respective biocompatibilities. The result of cell culture showed that nano-fiber could support the cellular growth and replication by developing 3-dimensional topography. Conclusion : Our results showed that increase in time of spinning and using plasma surface modification of nano-fibers by oxygen plasma would result in providing surface with the highest similarity to the extracellular matrix.
Masoumeh Akbari , Noor Amir Mozaffari , Hadi Peeri Dogaheh,
Volume 14, Issue 3 (10-2014)
Abstract
Background & objectives: Urinary tract infections (UTIs) caused by extended-spectrum beta lactamase (ESBL)-producing bacteria have become a growing problem worldwide. The aim of this study was to investigate the prevalence of ESBL-producing bacteria in urine samples of hospitalized patients in Imam Khomeini hospital of Ardabil over a period of October 2011 to August 2012. Methods : A total of 400 urinary pathogens isolated from urine samples were included in the study. All isolates were identified by routine biochemical methods and antimicrobial susceptibility testing carried out by Kirby-Bauer method. Confirmatory test for production of ESBLs was performed by the combination disk tests. The results were interpreted according to the recommendation of CLSI. Results : Of 400 isolated bacteria, 267 were E.coli, 39 Klebsiella pneumoniae, 17 Klebsiella oxytoca, 16 Enterobacter cloacae, 15 Enterobacter aerogenese, 6 Enterobacter agglomerans, 8 Enterobacter sakazakji, 3 Citrobacter froundi, 2 Citrobacter diversus, 3 Proteus mirabilis, 4 Edvardsiella tarta, 3 Serratia marcesecens and 17 Morganella morganii all of which then were analyzed. ESBL was detected in 36.75% (147) of isolates. Eighty nine E.coli cases (77.4%), 15 Klebsiella pneumonia (13.04%), 2 Klebsiella oxytoca (1.74%), 3 Enterobacter aerogenese (2.6%), 4 Enterobacter cloacae (3.5%), 1 Citrobacter ferundi (0.86%), and 1 Morganella morganii (0.86%) were detected as ESBLs producers, respectively. Conclusion : Based on the results of this study, broad-spectrum beta-lactamase production in bacterial strains isolated from patients with urinary tract infection was very high and almost 40% of all bacterial species isolates were ESBLs producers. Because of the high prevalence of ESBL-producing bacteria in the urinary tract infections in hospitalized patients of our area, we would strongly suggest that the ESBL production should be considered in these patients.
K Tahmasebi, M Jafari, F Izadi,
Volume 15, Issue 2 (7-2015)
Abstract
Background & objectives: Diazinon (DZN) as an organophosphate pesticide widely used in agriculture is associated with reducing the antioxidant capacity of the cell. Use of thiol compounds such as N-acetyl cysteine (NAC) as an antioxidant decreases oxidative stress in the cells. The aim of this study was to investigate the effect of NAC as an antioxidant against DZN- induced oxidative stress in rat brain and heart.
Methods: In present experimental study, male Wistar rats were randomly divided into four groups including: control group (corn oil as DZN solvent), DZN group (100 mg/kg), NAC group (160 mg/kg), and NAC+DZN, all of which were given intraperitoneally. 24 hours after injection, animals were anesthetized by ether, and the brain and heart tissues were quickly removed. After tissues hemogenation, superoxide dismutase (SOD), catalase (CAT), glutathione S-transferase (GST) and lactate dehydrogenase (LDH) activities, as well as glutathione (GSH) and malondialdehyde (MDA) levels were determined by biochemical methods.
Results: DZN increased SOD and GST activities and MDA level and decreased LDH activity and GSH content in brain and heart. Also, DZN increased CAT activity in the heart and increased it in the brain. Administration of NAC inhibited the change in these parameters.
Conclusion: DZN through free radical production leads to enhanced membrane lipid peroxidation, depleted GSH content and oxidative stress induction in the brain and the heart. Administration of NAC as antioxidant decreases the DZN-induced oxidative stress by scavenging free radicals and GSH synthesis, but its protection is not complete.
N Danesh Far , H Peeri Dogaheh, M Ghiamirad ,
Volume 15, Issue 2 (7-2015)
Abstract
Background & objectives: Resistant microbial strains are a serious threat to public health in different societies. A mong the extended-spectrum β-lactamases (ESBL) producing strains the Enterobacteriaceae family which is considered as the main factors producing urinary tract infections, have created many problems in treatment of this kind of infections. This study was conducted to determine the frequency of β-lactamase TEM-1 gene in Enterobacteriaceae isolated from urine samples in Ardabil city.
Methods: Within 6 months, 400 urinary isolates of Enterobacteriaceae of inpatients and outpatients were collected in Ardabil hospitals and were identified by standard methods. Antimicrobial susceptibility of isolates was tested by disk diffusion method, and ESBL producer confirmatory test was conducted using combined disk. Finally, the frequency of β-lactamase TEM-1 gene in producing extended-spectrum β-lactamases strains was investigated using PCR.
Results: From 400 isolates of Enterobacteriaceae, 150 cases (37.5%) were ESBL producing. PCR results showed presence of the TEM-1 gene in 69 cases (46%). The frequency of this gene in isolates of Enterobacter (Aerogenes, Cloacae), Klebsiella (Pneumoniae, Oxytoca) and E. coli was obtained to be 62.5%, 54.5% and 44.8%, respectively. Proteus mirabilis and Serratia marcescens strains were lacking these genotypes.
Conclusion: As regards the presence of TEM-1 gene, there is also increasing in other members of the Enterobacteriaceae family including Klebsiella and Enterobacter in addition to E. coli, therefore sufficient identification of this strains is necessary to prescribe the right medicine.
A Pourrahimghouroghchi, P Babaei, A Damirchi, B Soltanitehrani, S Ghorbanishirkoohi,
Volume 15, Issue 3 (10-2015)
Abstract
� Background & objectives: Obesity and visceral fat accumulation after menopause are associated with lipid profile changes, metabolic syndrome and c ardiovascular diseases. The aim of this study was to investigate the effect of 8-week aerobic - resistance training and estrogen replacement therapy on visceral fat and cardiovascular risk factors in ov ariectomized rats.
� Methods: Fifty female Wistar rats were ov ariectomized and divided into 5 OVX groups (n=10 rats per group): Ovx+sedentary (Sedentary), aerobic - resistance training (Ovx+Exe), aerobic - resistance training+estrogen replacement therapy (Ovx+Exe+Est), estrogen replacement therapy (Ovx+Est) and vehicle group or sesame oil (Ovx+Oil). The exercise consisted of aerobic-resistance training (20 m/min, 3 days/week, 60 min/day, 10% slope, Load 3% body weight) 17b-estradiol valerate (30 �g/kg bw in 0.2 ml sesame oil) were injected subcutaneously three days a week during 8 week. The co-treatment group received both exercise and estradiol protocol as same as previous groups.
� Results: After 8-week of interventions, visceral fat significantly reduced by Ovx+Exe and Ovx+Exe+Est compared to Sedentary rats (p<0.05), however no significant difference in body weight was observed. BMI significantly reduced in Ovx+Exe and Ovx+Exe+Est groups compared to Sedentary group. Also, BMI significantly reduced in Ovx+Est compared to Ovx+Oilgroup (p<0.05). Although this intervention changed lipid profiles, they were not statistically significant in neither of groups. Statistical comparisons between groups were performed usingone-way ANOVA test followed by Tukey&rsquos post-hoc test. The differences were considered significant at p<0.05.
� Conclusion: Eight weeks aerobic-resistance training successfully decreases visceral fat and BMI.
S Farid, H Peeri Dogaheh , M Ghiami Rad ,
Volume 15, Issue 3 (10-2015)
Abstract
Background & objectives: Drug resistance is one of the most problems in controlling microbial infections which assumes to be ever-increasing problem all through the world. Production of extended-spectrum &beta-lactamases enzyme (ESBLS) can cause a resistance to antibiotics of gram negative bacteria. The main purpose of this study was to determine antibiotic sensitivity patterns and SHV-1 genes frequency in collected urinary samples from hospitalized patients in Ardebil.
Methods: 400 isolated Enterobacteriaceae from urinary samples were recognized using an ordinary biochemical method. Antibiotic sensitivity test was conducted by Kirby and Bauer. The combined disk method was also utilized as a confirmatory test. The results were compared to clinical and laboratory standards institute (CLSI) standards. Finally, ESBL positive isolates were investigated by PCR method regarding the SHV-1 gene.
Results: From the total of 400 urinary isolates Enterobacteriaseae resistance to Ampicilin, Nalidixicacid, Co-trimoxazole, Cefotaxime, Ceftazidime, Ceftriaxone, Ciprofloxacin Ceftizoxime, Cefixime, Gentamycin, Imipenem were 82.5, 62.3, 67, 36, 49.5, 50.3, 52, 36, 41, 24.8 and 7.7 percents, respectively. 150 isolates (37.5%) were positive ESBL, and among all, 28 isolates (18.7%) contained SHV-1 gene.
Conclusion: According to obtained results from the study, regarding high percentage of resistance to antibiotics and high reduction of ESBLS in bacteria from who suffered from urinary infections, taking some logical steps for prevention seems to be essential.
Tayebeh Mohammadi, Mahmood Khaksary Mahabadi , Hosein Najaf-Zadevarzi , Zahra , Rezai ,
Volume 16, Issue 2 (7-2016)
Abstract
Background & objectives: Cyclophosphamide is one of the common drugs used for cancer treatment. Cyclophosphamide affects the placenta and it is teratogenic in pregnant women. The purpose of this study was to evaluate the protective effects of Mesna and Galbanum essential oil concurrent administration against cyclophosphamide toxicity in rat placenta tissue.
Methods: 22 pregnant female rats were randomly divided into 3 groups: (1) control (normal saline, n=7 rats), (2) cyclophosphamide (15mg/kg, n=7 rats), (3) cyclophosphamide (15mg/kg), Mesna (100mg/kg) and Galbanum (200mg/kg, n=8 rats). Medications were administered intraperitoneally on the 13th day of pregnancy. Then, rats were sacrificed on the 20th day of pregnancy. The placenta was separated and fixed after macroscopic evaluation. 5 µm tissue sections were prepared, stained and studied by light microscopy.
Results: Cyclophosphamide reduced the placental weight, thickness and the length of the small and large diameters. Histologically, it reduced the thickness of the labyrinth and basal layers, caused necrosis and congestion in these layers, reduced giant cells numbers and caused their nuclear shrinkage (p<0.05). Mesna and Galbanum oil had no effect on placental morphometry rather than cyclophosphamide (p>0.05) but histologically it could increase the labyrinth and basal layers thickness and also the giant cells numbers (p<0.05). It could also prevent nuclear shrinkage, giant cells and spongiotrophoblasts necrosis in the basal layer and reduce congestion in the labyrinth layer (p<0.05).
Conclusion: Mesna and Galbanum oil preserved the placenta of pregnant rat against toxic effects of cyclophosphamide probably by its antioxidant features and free radicals deletion.
Masoomeh Parvizi, Seyed Fazlollah Mousavi, Khadijeh Mohammadi , Mohsen Arzanlou,
Volume 16, Issue 3 (10-2016)
Abstract
Background & objectives: Streptococcus pneumoniae is one of the major causes of vaccine - preventable diseases worldwide. Current pneumococcal vaccines consist of serotype specific capsular polysaccharide antigen and do not offer full clinical protection against pneumococcal diseases. Due to such limitations, a new generation of protein-based pneumococcal vaccines is being developed. The objective of this study was to determine the distribution of gens encoding five protein antigens including pneumococcal histidine triad D and E (phtD, phtE), rlr- regulated gene A (rrgA), Autolysin (lytA) and Pneumococcal surface protein C (pcpC) among pneumococcal isolates collected from nasopharyngeal specimens in healthy children.
Methods: A total of 43 pneumococcal isolates were collected from nasopharyngeal specimens of healthy children attending the kindergartens in Ardabil province. The strains were identified using optochin susceptibility and bile solubility testes and further confirmed by amplification of capsular polysaccharide A gene (cpsA). PCR was used for screening the presence of pcpC, phtD, phtE, rrgA and lytA genes.
Results: 81.4 % of isolates were found to contain at least one of the tested genes. lytA, pcpC, phtE, phtD and rrgA were detected in 70, 60, 39.5, 35 and 25.5 percent of isolates, respectively. The results showed that the genes were not distributed consistently among the isolates and for obtaining a full coverage pneumococcal vaccine, multiple choices of these antigens should be included.
Yavar Mahmoodzadeh , Mohammad Mazani, Lotfollah Rezagholizadeh, Aliasghar Abbaspour, Eslam Zabihi, Pirooz Pourmohammad,
Volume 16, Issue 4 (1-2016)
Abstract
Background & objectives: Some herbs contain compounds with antioxidant activity and can be used to protect or cure damages caused by chemical toxins. The aim of this study was to evaluate the effects of carbon tetrachloride (CCl4) on total antioxidants in various tissues and Tanacetum parthenium impacts on reducing devastating effects of carbon tetrachloride.
Methods: A total of 42 male Wistar rats were divided into seven groups of six animals in each group: normal control, damaged control, three groups that treated with 40, 80, and 120 mg/kg of Tanacetum parthenium extract 14 days before CCl4 injection and two groups served as post-treatment groups that received 80 and 120 mg/kg extract 2, 6, 24, and 48 h after CCl4 injection. At the end of study the liver, kidney, testis, and heart were removed and then homogenized and then the antioxidant activity of the tissues assessed using FRAP method. Results were analyzed by one-way ANOVA test.
Results: The results showed that the injection of carbon tetrachloride significantly decreasestotal antioxidant in both liver (p<0.001) and kidney (p<0.05) tissues. Administration of extract significantly (p<0.05) increased the total antioxidant of liver and kidney.
Conclusion: Protective effect of Feverfew against CCl4 induced damages is more effective in liver and kidney than testis and heart..
Hasan Edalatkhah, Nazila Rahnema Tareghi , Saeeid Sadeghieh Ahari , Ali Nemati,
Volume 17, Issue 2 (7-2017)
Abstract
Background & objectives: Acne Vulgaris is a chronic inflammation of pilosebaceous glands. Some studies have indicated the significant decrease of linoleic acid in the sebum composition of the patients with acne. The aim of this study was to determine the probable therapeutic effects of linoleic acid on moderate acne.
Methods: In a double-blind randomized clinical trial, 40 women with moderate acne were divided into two groups, each group with 20 each with 20 samples. After recording the nutritional and demographic factors, the case group received, 100 mg oral doxycycline daily plus 3 gr conjugated linoleic acid and the control group received 100 mg doxycycline daily plus 4-3 oral paraffin capsules as placebo for one month. The number of acne lesions were counted before and after treatment and the mean of Acne Severity Index (ASI) and Global Acne Grading System (GAGS) were determined for each group. The collected data were analyzed by SPSS software using chi-square, independent t-test and paired t-test.
Results: The mean age was 22.8±5.5, in the case group and21.7±5.1 in placebo group indicating no statistically significant deference (p=0.51). At the end of treatment there was no statistically significant deference between the two groups in decreasing acne (p=0.31). During the treatment, ASI reduced from 129.5±13 to 77±11.6 in the case group and from 132±18.6 to 67.5±11.6 in the placebo group, –but there was no statistically significant deference between the two groups (p=0.37).
Conclusion: It seems using 3 gr oral conjugated linoleic acid daily for 1 month has no effect on remission of acne.
Mehrzad Saraei , Laleh Ghanbarzadeh, Elham Hajialilo , Tahereh Barghandan, Ms Farzanrh Amini, Meysam Sharifdini,
Volume 18, Issue 4 (1-2018)
Abstract
Background & objectives: Trichostrongylus spp. are common parasites of herbivorous animals and trichostrongyliasis is an important zoonotic disease in Iran, especially in northern Iran. Routine laboratory methods for diagnosis of these infections are direct smear and formalin-ethyl acetate techniques. There is no enough evidence about the sensitivity of nutrient agar plate culture for detection of Trichostrongylus spp infections. The objective of this study was to compare the nutrient agar plate culture and the formalin-ethyl acetate sedimentation techniques for laboratory diagnosis of human trichostrongyliasis in an endemic area of northern Iran.
Methods: A total of 1553 fresh stool samples were collected from residents of 31 villages within the Fouman district in Guilan province during 2015–2016. All samples were examined using nutrient agar plate culture and formalin- ethyl acetate concentration techniques for detection of Trichostrongylus spp. infections.
Results: Overall, 71 cases were diagnosed with Trichostrongylus spp. by at least one of the methods. Considering parasitological results as the diagnostic gold standard, the sensitivity and specificity of nutrient agar plate culture were 90.1% and 100%, respectively. Also, the sensitivity and specificity of the formalin ethyl acetate concentration method were 95.8% and 100%, respectively.
Conclusions: According to the results of this study, the formalin ethyl acetate method is more sensitive than nutrient agar plate culture in the diagnosis of human trichostrongyliasis, especially in humid climate regions such as North of Iran.
Ali Soleimanzadeh, Ali Shalizar Jalali, Abdulrahim Abdullahi, Mohammad Moein Sabzeie,
Volume 20, Issue 2 (7-2020)
Abstract
Background & objectives: The present study investigated the effects of royal jelly on lead acetate induced toxicity on sperm parameters, reproductive hormone assay, and bak gene expression in NMRI male mice.
Methods: In this study, fifty four male mice were randomly divided into nine groups: control group (without royal jelly) (n=6); sham group(10 ml normal saline) (n=6); lead group (1000 ppm, oral) (n=6); Group 4: royal jelly (100 mg/kg/day, oral) (n=6); Group 5: royal jelly (250 mg/kg/day, oral)(n=6); Group 6: royal jelly (500 mg/kg/day, oral)(n=6); Group 7: royal jelly (100 mg/kg/day, oral) + 1000 ppm lead (n=6); Group 8: royal jelly (250 mg/kg/day, oral) + 1000 ppm lead (n=6) and Group 9: royal jelly (500 mg/kg/day, oral) + 1000 ppm lead (n=6). On day 35, blood samples were collected from anaesthetized mice by cardiac puncture to assess reproductive hormones and the testes were harvested for determination of sperm parameters and expression bak gene. Sperm parameters including motility, viability, DNA damage, morphology and total antioxidant capacity (TAC) levels were determined.
Results: The results showed that administration of royal jelly significantly enhanced sperm parameters and all reproductive hormone levels compared to control mice, (p<0.05). Also, treatment with lead acetate caused a significant reduction in levels of all reproductive hormones and a significant diminution in sperm motility, morphology, viability; with an increase in percentage of dead spermatocytes (p<0.05). The co-administration of the 250 and 500 mg/kg/day royal jelly with lead acetate could ameliorate the deleterious effects of lead acetate resulting in a significant increase in sperm parameters and all reproductive hormones and increase the total antioxidant capacity (TAC) levels (p<0.05). Also, the expression of bak gene in all treated (sham, royal jelly groups) and control groups was significantly lower than the lead acetate group (p<0.05).
Conclusion: In conclusion, our findings suggest that the royal jelly has a beneficial effect on male reproductive parameters following lead acetate induced toxicity in mice.
Bahman Ebrahimi-Torkamani , Marefat Siahkouhian, Ali Khazani, Anoshirvani Sajad, Bolboli Lotfali, Maryam Asadi,
Volume 20, Issue 3 (10-2020)
Abstract
Background & objectives: Over the last couple of decades, research has focused on attempting to understand the genetic influence on sports performance. Whole exome sequencing (WES) has great potential to explore all possible genetic variants associated with physical performance. However, this new technique has not been used in sports genetics research. Therefore the aim of this case study was to report new variants in genes involved in skeletal muscles strength (ACE, NOS3, IGF1R, IL-6) in an elite weightlifter using WES.
Methods: After obtaining a written testimony from a 32-year-old man, who is a member of the Iranian national weightlifting team, 5 cc of blood was taken and then DNA was extracted. DNA samples were analyzed using the whole exome sequencing method.
Results: Whole exome sequencing analysis revealed a missense variant (99454613G>A) in IGF1R, 22771156C>T polymorphism in IL-6, 150695726T>C and 150704250C>G polymorphisms in NOS3 and 61573761T>C and 61564052A>G in ACE genes.
Conclusion: Results of whole exome sequencing analysis revealed a missense variant and polymorphism in muscle strength and hypertrophy related genes in this elite weightlifter. However, other studies with larger sample sizes are needed to investigate the relationship between these variants and power athlete status.
Hamid Reza Shetabi, Dariush Moradi Farsani, Mohammad Razani,
Volume 21, Issue 1 (4-2021)
Abstract
Background & objectives: The aim of this study was to evaluate the effects of preventive administration of ketamine, fentanyl and paracetamol on pain intensity and hemodynamic parameters after deep vitrectomy.
Methods: In this clinical trial, 80 candidates of deep vitrectomy surgery were randomly assigned into 4 groups (1-4) and received 0.5 mg / kg ketamine, 2 μg / kg fentanyl, 10 mg/kg of paracetamol and equivolume of normal saline respectively intravenous infusion during the last 15 minutes of surgery. Pain intensity and hemodynamic parameters were recorded and analyzed.
Results: Pain intensity was significantly higher in placebo group during the first 4 hours after operation (p<0.01), but no significant differences were observed between the study groups at 8 and 24 hours after operation in this regard. There were no significant differences between the three intervention groups in terms of pain intensity during the study. Also there were no significant differences between the 4 study groups regarding to hemodynamic parameters.
Conclusion: The use of each of the three intervention drugs significantly reduced the pain intensity compared to the control group without any hemodynamic derangement.
Aida Nahumi, Maryam Peymani, Asadollah Asadi, Arash Abdolmaleki, Yasin Panahi, Mohammad Ali Shahmohammadi,
Volume 23, Issue 4 (1-2024)
Abstract
Background: Identifying protein interactions is one of the main challenges in the fields of biostructure and molecular biology. Despite extensive progress, the exact patterns of protein-protein interactions are still unknown. The main goal of this study is to computationally evaluate the interactions of fibronectin-1 in the extracellular matrix of decellularized trachea and integrins in adipose tissue stem cells in order to provide the most accurate possible visualization of these interactions and their role in biological processes.
Methods: After decellularization of the sheep trachea through the detergent-enzyme method, histological evaluations and ultrastructure photography of the samples were done by scanning electron microscopy. Also, the simulations of fibronectin1 binding of extracellular matrix protein with integrin αvβ1 and α5β3 of stem cells derived from adipose tissue were investigated, and interaction energy analysis was applied to predict the structure of protein-protein complexes using the algorithms available in HDOCK and ClusPro servers.
Results: The findings indicated the preservation of extracellular matrix components and scaffold ultrastructure. Also, in order to find the most favorable connection states in terms of energy, some of them were reported as stable interactions among the top types of connections. This insight provides a valuable understanding of cell-matrix adhesion, migration, and signaling, with potential implications for therapeutic development.
Conclusion: The prepared scaffolds are ideal for engineering applications for which computational analysis and experimental data have been used for visualization of stable connection states with energy efficiency between fibronectin and integrin. Also, more studies on cell adhesion modeling in connection with tissue engineering science can provide a suitable field for the development of regenerative medicine in further studies.
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