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  Background & objective : Recovery state or post - anesthetic awakening is one of the most dangerous anesthetic stages . Respiratory, cardiovascular and other complications such as nausea, vomiting, shivering, pain and restlessness have been reported . This study designed to investigate prevalence of this complications and its probable risk factors in Ardabil Fatemi and Alavi hospitals.

  Methods : In this prospective study, 160 patients were selected in Alavi and Fatemi hospitals during winter of 2001. We completed a sheath for everybody about their history of medical diseases, pervious history of anesthesia, addiction, drug abuse, smoking, physical class of ASA, type of anesthesia, time and place of surgery . Data was analyzed by SPSS software.

  Results : These results were obtained in patients after surgery in recovery room: shivering
36.3 % , pain 26.9 % , restlessness 21.3 % , respiratory complications 13.8 % , cardiovascular complications 12 % , nausea and vomiting 8.8 % . We found significant correlation between some risk factors with complications, e. g: shivering with history of medical diseases, drug abuse and place of surgery and so, pain with restlessness, respiratory complication and place of surgery .

  Conclusion : This study according to similar studies revealed higher prevalence of some of post - anesthetic complications as shivering, pain and restlessness in our samples .

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  Background & Objective : Diabetes is one of the most important world health problems and a chronic disease that needs continuous care. Therefore, these kinds of patients should take self- care education. This study is an attempt to investigate the effect of nutritional education on blood sugar level and macronutrients intake in IDDM patients.

  Methods : 24 IDDM patients under 20 were selected for educational program on nutrition. The data were collected two times using food questionnaire (24- hour recall) and blood sugar level before and after education was estimated. The data were analyzed by paired t-test, using SPSS and Food Processor.

  Results : There was not statistically significant relationship between blood sugar level before and after education. Also the results showed that there was statistically significant relationship between macronutrients intake before and after education (P=0.35). After education, macronutrients were similar to WHO recommendations. HbA₁C level in most of the patients was 9-12% which refers to the moderate control of this diseases and hypoglycemia decreased from 4.2% to 3%.

  Conclusions : There were no remarkable changes in blood sugar but the amount of HbA₁C and Macro Nutrients showed that education had a positive effect on patients.

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  Background & Objectives: Tina capitis is among the most prevalent human infections induced by dermatophytes. Some dermatphytic factors are globally widespread while some others are geographically determined. A number of similar researches have been conducted in Iran however, due to the geographically diverse etiology of the disease and lack of similar research in Meshkin Shahr, the present study was conducted to determine the prevalence of tinea capitis in primary schools of this city.

  Methods: In order to determine the frequency of the tinea capitis in the students of primary school in Meshkin Shahr, children in 13 schools including 2470 boys and 3012 girls were studied. For this purpose first of all the children’s scalps were observed clinically and the specimens such as hair and scale were taken from 228 pupils. The specimens were examined by direct and culture methods.

  Results: Of all the subjects, 38 students (0.7%) had tinea capitis. The prevalence rate was 0.8% and 0.6% among boys and girls respectively, which was statistically significant. The high degree of disease was seen in 11 year old students (39.5%) and the main source of infection was Trichophyton violaceum.

  Conclusion: Desptie a decrease in tinea capitis among children especially in primary school pupils nationwide, there is still a considerable (0.7%) rate of prevalence in Meshkin Shahr which demands attention.

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Background & Objective: Visceral leishmaniasis (kala-azar) is an infectious-parasitic disease occurring sporadically in various parts of Iran. The disease is endemic in Ardabil, East Azarbaijan, Bushehr and Fars provinces. This study was performed with the aim of evaluation and comparison of direct agglutination test (DAT), indirect immunofluorescent antibody (IFAT) and ELISA in sero-diagnosis of visceral leishmaniasis that is following a seroepidemiological survey in Germi district of Ardabil province.
Methods: Cluster sampling has been performed from ≤12 years old and 10% of the adult population in Germi district. Altogether 1155 blood specimens were collected to detect anti- Leishmania antibodies. The samples were tested by direct agglutination test (DAT), indirect immunofluorescent antibody (IFAT) and ELISA methods.
Results: 32 (2.8%) of the collected specimens have anti-Leishmania antibodies with titers ≥1:800 and from total specimens 7 (0.6%) were positive with ≥1:3200 titers. In IFA test 32 (2.8%) have titers ≥1:40 and from total specimens 6 (0.52%) were positive with ≥1:320 titers. In ELISA test 8 specimens were positive and other specimens were negative.
Conclusions: According to the results of this study, IFAT and ELISA are specific and high sensitive tests for sero-diagnosis of visceral leishmaniasis but these methods are expensive and require the complicated tools. Whereas, DAT is a simple method, not expensive and require a simple local laboratory with one or two trained technicians. Therefore DAT could be used for seroepidemiological studies and sero-diagnosis of visceral leishmaniasis in endemic areas instead of IFA and ELISA methods.

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  Background & Objectives: In endemic area, chemically fighting with carrier of kala azar disease is one of the main ways for reducing disease, and ficam is one of the toxins that is appropriate to eliminate mosquitoes and is from health poison among the pesticides. Therefore, by considering the lasting in nature, using ficam can greatly destroy disease-carrying mosquitoes.

  Methods: In this cross - sectional study the rate of effect ficam on different vectors species were examined in Meshkinshahr city. First two infected villages, as cases and controls, and 10 households from each village selected and 1000 m² from the case village was spraying with ficam than 0.3 g /m². Then, using sticky traps set in the distances were attempted to identify species and Fon (per 2 villages).

  Results: In the rate of 524 sand fly predating in this study included 70.5% Phlebotomus Kandelakii, 5% F. Papatasi, 7.5% F. Major, 7.5% F. Balcanicus, 9.5% F. longidoctus. In the case village was spraying and , respectively, 3-15 - 30 - 45-60 -75 and 90 days after spraying at about 79.5% - 69.5% - 83% - 90% - 93% - 100% and 100 % sand fly vanished.

  Conclusion: According to this study can be said that ficam poison with the survival of three months in nature and with average of lethality coverage of appropriate terms of season to prevent from disease Kla-azar and eliminate sand flies and as a poison, appropriate of health before starts up the season of carriers activity. And ficam poison can be used in the whole of region and country.

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  Background & Objectives: Giardia duodenalis is the most common intestinal parasite with cosmopolitan distribution. This parasite has been found in the intestine of humans and other mammalian hosts including cats, dogs, cattle, sheep, deer, pigs and muskrats. It is postulated that animals maybe reservoir for human infection and viceversa. In present study, the possible genetic similarity between cat and humans Giardia and its probable zoonosis were investigated.

  Methods: Direct examination and formalin-ether concentration techniques were performed on stray and semi stray cat fecal specimens. Gradient sucrose method was applied for collection and purification of cysts and DNA extraction was performed by phenol-chloroform and CTAB (Cetyl trimethyl ammonium bromid ( methods. DNA of cysts could hardly be extracted after repeated freezing and thawing. Polymerase chain reaction (PCR) was performed for DNA amplification. In this study triosephosphate isomerase (tpi) gene was selected as a molecular marker. Two sets of primers (PM 290 and PM 924) were considered. Two restriction enzymes RsaI and AvaI were also used to determine restriction fragment length polymorphism (RFLP) for PCR fragments amplified by both primer sets.

  Results: Ten samples were positive for Giardia cysts which were examined for molecular investigation. Four cat isolates were amplified by PM 290. PCR-RFLP patterns were found to be similar to human isolates AC≠AF 069556 (subgroup of AC≠U 57897) with possibility of cross-transmission.

  Conclusion: Therefore the similarity of genomic characters of isolates of cat and human Giardia implies possibility of zoonosis and transmission of these protozoa from cat to human and vice versa.

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  Background & Objectives : Visceral leishmaniasis is an important endemic disease in Iran and Meshkin shahr County is the most important foci of visceral leishmaniasis in Iran. The aim of this study is the survey of recent changes in epidemiological patterns of visceral leishmaniasis in Meshkin Shar area during 12 years.

  Methods : This is a descriptive retrospective study. Records of 193 cases with confirmed kala-azar disease were reviewed in the period of 2001-2012. Data collected from files and were analyzed using descriptive statistics and SPSS v.15.

  Results : From 193 patients with confirmed kala-azar disease, 85% were resident in rural and 15% in urban areas. Among of these patients, 43% were female and 57% male. About 66% of patients were under 2 years old, 23% in age group between 2-5 years and 11% above five years. Among four parts of Meshkin Shahr County, in the first five years of the study, the most cases of the disease were from the central part, but in second six years the most cases were from the west part of this county and in latest year (2012) it has been coverted .

  Conclusions : Incidence of kala-azar was decreased during last 12 years. Because of high infection rate in dogs and considering important role of these reservoirs in conserving and distribution of disease, stopped health activities in any parts of area can increase the incidence of disease.

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Background & objectives: Visceral leishmaniasis is a zoonotic disease and is considered as the most important disease in dogs. The disease has been reported in North West and South of the country, in Iran. In addition to stray dogs, some apparently healthy dogs can be contaminated without showing any sign or symptoms in this area. In the present study, canine visceral leishmaniasis was investigated in dogs lacking clinical signs in Meshkinshahr city in Ardabil, Iran.

Methods: This study was a cross-sectional survey conducted during 2011-2014. A total of 110 serum samples collected from dogs either having or lacking clinical signs and tested by direct agglutination test (DAT) assay. Then 10 dogs (41.6%) showing clinical signs and 15 dogs (17.4%) without any symptoms were autopsied and their spleens were sampled. The samples were used for smear preparation and culturing.

Results: Based on the results, among 24 dogs with clinical signs 20 cases (83.3%) and of 86 dogs without signs, 16 cases (18.6%) found to be positive. On the other hand, smear and culture results were found to be positive in 100% and 60% of dogs with and without clinical signs, respectively. The interesting and impressive results of this study was that the dogs with symptoms but negative DAT and asymptomatic dogs with negative DAT were positive in parasitological tests.

Conclusion: This proves that asymptomatic dogs like symptomatic dogs can be effective in L. infantum infection and is able to maintain the transmission of the disease in endemic areas. On the other hand, a number of symptomatic dogs with negative anti-leishmania antibodies were positive in parasitological tests. Thus, this study also shows that although DAT is effective in determining asymptomatic dogs and canine visceral leishmaniosis control programs but it does not seem to be satisfying in endemic areas such as Meshkin-shahr. Thus in endemic areas, it is recommended that the low antibody titer should be considered.

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Background & objectives: Early diagnosis of preeclampsia and its severity in pregnant women is greatly important for controlling this disease and preventing subsequent dangers for mother and the fetus. Current study is conducted to assess neutrophil-lymphocyte ratio (NLR) as a determining factor for the severity of the disease in patients with preeclampsia referring to Sabalan hospital, Ardabil.

Methods: This was a case control study on 50 pregnant women with severe preeclampsia (group 1), 50 pregnant women with mild preeclampsia (group 2), and 50 healthy pregnant women (group 3). The groups were homogenized for basic variants including age, gestational age, mean BMI, gravid and parity. Required information including age, gestational week, BMI, gravid, parity, 24-hour urine protein, systolic and diastolic blood pressure, and NLR were obtained and analyzed.

Results: The NLR was significantly higher in women with severe preeclampsia compared with mild preeclampsia (p=0.031) and healthy women (p<0.001). NLR did not show difference between mild preeclampsia and healthy women (p=0.209). Significant positive correlation was also observed between NLR and proteinuria (p<0.0001, r=0.558), systolic blood pressure (p=0.0026, r=0.244), and diastolic blood pressure (p=0.0028, r=0.242).

Conclusion: This study showed that maternal NLR can be used as a marker for preeclampsia severity.

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Background & objectives: The growing numbers of cosmetic treatments, especially Botox in recent years, and the limited researches on the mental characteristics of those applying for this type of cosmetic surgery, have necessitated more attention to this matter. The aim of this study was to compare appearance schema and emotional processing in applicants and non-applicants of botox injection.
Methods: the present work was cross-sectional (causal-comparative) study. The statistical population of the study consisted of all individuals referring to beauty clinics (dermatology, hair, beauty) in Ardabil, second half of 1396. Among them, 60 subjects (30 applicants and 30 non-applicants for Botox cosmetic) were selected through convenience sampling procedure. Furthermore, Appearance Schema Inventory (ASI) and Baker et al’s Emotional Processing Questionnaire were used to collect the data. The data were analyzed using multivariate analysis of variance (MANOVA).
Results: The results showed a significant difference between the mean of appearance schema and emotional processing in applicants and non- applicants (p<0.01). The amount of visual schema (self-assessment and self-motivation) and emotional processing was high in the Botox group (p<0.01) and non-Botox group (p<0.01) respectively.
Conclusion: results indicated that Botox and beauty enthusiasts had high appearance schemas and low emotional processing. These people in compared to non-applicants were more likely to experience difficulties.
 

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Background & Objectives: Cold Plasma is an emerging non-thermal, chemical-free, environmentally friendly disinfection technology. Plasma-activated water has received considerable attention from researchers in recent years. Despite extensive studies on the antibacterial effects of plasma-activated water, its anti-eukaryotic effects have not been identified. In humans, Acanthamoeba causes granulomatous encephalitis, skin ulcers, and Acanthamoeba keratitis. Considering the health importance of Acanthamoeba, this study investigated the anti-amoeba  effect of plasma-activated water on trophozoites and cysts of Acanthamoeba castellanii.
Methods: In this study, plasma-activated water prepared by the cold atmospheric plasma method.Physicochemical properties of produced water were evaluated by measuring pH, hydrogen peroxide, nitrite, and nitrate. To assess the effect of plasma-activated water on A. castellanii, trophozoites and cysts were exposed to plasma-activated water for 0.5, 1, 2, 3, 4, and 5 hours. Three replicates were examined each time. At the mentioned times, cell viability was calculated by trypan-blue staining and counting on a hemocytometer, and the results were statistically analyzed.
Results: Based on the physicochemical results, the mean pH of plasma-activated water in this study was about 3.4, and the amount of hydrogen peroxide, nitrate, and nitrite were 102, 737, and 36.94 μM, respectively. The present study showed that plasma-activated water killed A. castellanii trophozoites after three hours of exposure and A. castellanii cysts after four hours of exposure. On the other hand, some trophozoites gradually became cysts after exposure to plasma-activated water. These cysts became more resistant to plasma-activated water and inactivated after five hours of exposure.
Conclusion: In this study, for the first time, the effect of plasma-activated water on A. castellanii was investigated. The results of the present study showed that plasma-activated water is able to inactivate A. castellanii trophozoites and cysts. Therefore, plasma-activated water can be used to disinfect and inactivate A. castellanii.

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Background & objectives: Menopause and especially acute menopause due to surgery is associated with many complications in women. The aim of this study was to determine the effects of genistein and regular swimming exercise (alone/or in combination) on pain through a possible mechanism of inflammation and oxidative stress in ovariectomized rats.
Methods: In this study, rats were divided into six groups, including: control, sham, ovariectomy (OVX), ovariectomized with eight weeks of swimming exercise training (OVX.E), ovariectomized with eight weeks of genistein administration (OVX.G), and ovariectomized with eight weeks of combined treatment (OVX.G.E). The effects of genistein and/or exercise were evaluated by examining the pain intensity with tail-flick and formalin tests. The serum levels of tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), total antioxidant capacity (TAC), total oxidant status (TOS), and malondialdehyde (MDA) were also evaluated by ELISA and spectrophotometer.
Results: In the OVX group compared to the control group, tail-flick and formalin tests showed an increase in pain response. Also, a significant increase in the serum levels of IL-1β, TNF-α, MDA, TOS and a decrease in TAC was observed in the OVX group, however, in the OVX.E, OVX.G and especially OVX.E.G groups, pro-inflammatory cytokines and oxidative stress as well as pain responses showed a significant decrease compared to the OVX group.
Conclusion: A combination of genistein and regular swimming exercise was synergistically more effective in reducing acute and chronic pain than using them alone in the postmenopausal period.