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Showing 3 results for Shamsazar
A Shamsazar, A Asadi, F Shamsazar,
Volume 15, Issue 3 (autumn 2015)
Abstract
Background & Objectives: Maintain of fixed amount of glucose in blood has always been valuable. Especially in diabetic patients it is important to determine accurate amount of it. In this study we are trying to find a method to determination of the amount of serum glucose.
Methods: First a biosensor was prepared using carbon paste electrode modified with Copper Oxide nanoparticles for determination of serum glucose. The synthesized Copper Oxide nanoparticles were studied by using a variety of chemical spectrum UV-Vis, XRD, SEM and TEM. XRD confirmed that our synthesized are Copper Oxide nanoparticles. By measuring of the oxidation currents in the several levels of glucose, the accuracy and efficiency of biosensor was studied.
Results: Based on a increase of the electrocatalytic response of the oxidized form of GOD to dissolved oxygen, the proposed biosensor exhibits a linear response to glucose concentrations ranging from 0 to 15 mM with a detection limit (defined as the concentration that could be detected at the signal-to-noise ratio of 3) of 9 �M at an applied potential of 0.44 V which has better biosensing properties than those from other biosensors. This biosensor retained 89% of its initial response after 30 days storage at pH=7.
Conclusion: Carbon paste electrode and Copper Oxide nanoparticles offer excellent catalytic activity toward hydrogen peroxide generation in enzymatic reaction between glucose oxidase and glucose, which would enable sensitive determination of glucose. This biosensor exhibited good stability, reproducibility and low interferences. It has been used to diagnose diabetes very fast and sensitively and to determine the glucose concentrations in serum samples with satisfactory results.
Abbas Sabahi Namini, Arash Abdolmaleki, Sharareh Mirzaee, Mehrdad Sheikhlou, Asadollah Asadi, Ali Shamsazar,
Volume 21, Issue 2 (summer 2021)
Abstract
Background & objectives: Research on intelligent nanomaterials that accelerate the process of nerve regeneration and treatment by different methods such as antioxidant effects, stimulation of nerve cell proliferation, modulation of the immune system and inflammatory factors is great importance. The aim of this study was to prepare cinnamon-coated cerium oxide nanoparticles and evaluate its antioxidant and cytotoxicity effects on PC12 cell line.
Methods: Cinnamon-coated cerium oxide nanoparticles were prepared and their structural properties were evaluated by transmission electron microscopy (TEM) and Fourier transform infrared spectroscopy (FTIR). To evaluate the antioxidant properties of the compounds, free radical trapping methods 2 and 2 diphenyl-1-picryl hydrazyl were used. Cell viability in the presence of compounds was measured by a toxicity test (MTT) [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide]. Data were analyzed by one-way analysis of variance and Tukey's post hoc test.
Results: FTIR spectra and TEM images showed the processing of nanoparticles with an average size of less than 100 nm with cinnamon coating on their surface. Also, the antioxidant capacity of cinnamon-coated cerium oxide nanoparticles was significantly higher (*p<0.038) than extracts and nanoparticles alone at similar concentrations. Evaluation the results of cytotoxicity showed that the lowest toxicity was observed in the cinnamon-coated cerium oxide nanoparticles group.
Conclusion: Results showed higher antioxidant properties and low cytotoxic effects of cinnamon-coated cerium oxide nanoparticles compared to other groups, which leads to better efficacy, proliferation, longer cell survival, its green synthesis and coverage by cinnamon
Masoomeh Saboorifar, Ali Shamsazar, Asadollah Asadi, Mostafa Shourian,
Volume 23, Issue 3 (Autumn 2023)
Abstract
Background: Determining the concentration of hydrogen peroxide in liquids and biological samples is very important because of its effects on human health. This study aimed to design a new electrochemical biosensor based on hemoglobin to detect hydrogen peroxide in serum samples.
Methods: In this study, a basic science, a biosensor based on modifying the glassy carbon electrode surface with a nanocomposite consisting of cobalt oxide nanoparticles and multi-walled carbon nanotube functionalized with a carboxyl group (MWCNT/Co3O4) and hemoglobin stabilized on this nanocomposite was made as a biological recognition element.
Results: In optimal conditions, the biosensor was used to measure different concentrations of hydrogen peroxide. The designed biosensor showed a wide linear response range from 10 μM to 500 μM, a detection limit of 0.512 μM, and high reproducibility and stability.
Conclusion: In this innovative research work, MWCNTs/Co3O4 nanocomposite was used to make a diagnostic biosensor. The presented biosensor showed an acceptable performance in the measurement of hydrogen peroxide in serum samples and laboratory solutions.
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