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Showing 3 results for Salehi
Rezvan Zabihollahi , Maryam Nourmohammadi , Azar Farhang Esfahani, Rohollah Vahabpour, Seiyed Mahdi Sadat , Mohammad Reza Aghasadeghi , Mansour Salehi , Seiyed Davar Siadat ,
Volume 12, Issue 1 (spring 2012)
Abstract
Background & Objectives : Several studies have been conducted to explore anti-HIV drugs. Discovery and study of novel anti-HIV-1 compounds need live viruses and has serious biosafety concerns. In this research we reported a novel and safe system for assaying the cytopathic effects of HIV by using single cycle replicable (SCR) HIV-1 virions. Methods: To produce the SCR HIV-1 virions, pMD2G, pmzNL4-3 and pSPAX2 plasmids were co-transfected into HEK293T cells. Different amount of SCR virions were used to infect target cells (MT-2). Within the infected cells, the number of formed syncytia was counted under the light microscopy. The lethal effects of the SCR HIV virions were measured using XTT proliferation assay. Results: Formation of syncytia among SCR HIV infected cells was detectable 24 hours after infection. Highest amount of syncytia was seen 72 hours after infection. Increase in the amount of virions caused increasing of syncytia and the cytopathic effects of SCR HIV-1. Infection with more than 1600ng P24 SCR HIV decreased the syncytium formation and viability of all cells. The calculated IC50 (50 percent inhibitory capacity) for nevirapine and BMS806 using this method was 50nM and 30nM, respectively. Conclusion: SCR HIV-1 virions are replicable only for one cycle. Using these virions can improve the safety of HIV researches. Herein, we optimized the assaying of HIV induced cytopathic effects by using SCR HIV-1 (NL4-3) virions. The accuracy of this method was accepted by quantifying the anti-HIV-1 effects of nevirapine and BMS806 by (SCR) HIV-1 virions.
Ali Niapour, Zahra Taghipour, Sahar Kiani, Fereshteh Karamali, Nazila Niapour, Mohammad Mehdi Mir Hosseini, Mohammad Reza Piri, Hosein Salehi, Noruz Najafzadeh, Mohammad Hosein Nasr-Esfahani ,
Volume 13, Issue 4 (Winter 2013)
Abstract
Objective: Spinal cord injury (SCI) has become an especially challenging target in experimental neuroscience. Approach into the spinal cord is the interface among all different types of spinal cord injury modeling. The lower thoracic spinal cord has generated special interest due to the lower limbs’ spinal pattern generator position and presence of relative scales for behavioral assessment. However, a clear method with which to approach the thoracic spinal cord has yet to be determined. Methods : A total of 20 animals were subjected to this study. Following induction of anesthesia, the 10th thoracic vertebra were positioned, and muscles were retracted. Using the high speed rotary, the vertebral lamina were carefully thinned. As a final point, the reduced lamina was meticulously removed away to expose underlying spinal cord. Loco motor behavioral test (BBB) was implemented before and after surgery procedure. Results: This manuscript has presented the stepwise method to expose rat thoracic spinal cord. Whole procedure took less than an hour. Animals acquired complete BBB loco motor rating score before and after surgery indicating the safety of procedure. Conclusion: This article introduces simple and practical approach for the rat lower thoracic spine. The anatomical orientation, anesthesia , postoperative management , and common problems are discussed .
Parisa Habibi, Hadi Yousefi, Mehdi Khazaei, Mohammad Zarei, Iraj Salehi, Reza Jamali Delfan, Simin Afshar,
Volume 22, Issue 2 (Summer 2022)
Abstract
Background & objectives: Menopause and especially acute menopause due to surgery is associated with many complications in women. The aim of this study was to determine the effects of genistein and regular swimming exercise (alone/or in combination) on pain through a possible mechanism of inflammation and oxidative stress in ovariectomized rats.
Methods: In this study, rats were divided into six groups, including: control, sham, ovariectomy (OVX), ovariectomized with eight weeks of swimming exercise training (OVX.E), ovariectomized with eight weeks of genistein administration (OVX.G), and ovariectomized with eight weeks of combined treatment (OVX.G.E). The effects of genistein and/or exercise were evaluated by examining the pain intensity with tail-flick and formalin tests. The serum levels of tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), total antioxidant capacity (TAC), total oxidant status (TOS), and malondialdehyde (MDA) were also evaluated by ELISA and spectrophotometer.
Results: In the OVX group compared to the control group, tail-flick and formalin tests showed an increase in pain response. Also, a significant increase in the serum levels of IL-1β, TNF-α, MDA, TOS and a decrease in TAC was observed in the OVX group, however, in the OVX.E, OVX.G and especially OVX.E.G groups, pro-inflammatory cytokines and oxidative stress as well as pain responses showed a significant decrease compared to the OVX group.
Conclusion: A combination of genistein and regular swimming exercise was synergistically more effective in reducing acute and chronic pain than using them alone in the postmenopausal period.
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