|
|
|
Search published articles |
|
|
Showing 20 results for Saleh
Farhad Salehzadeh , Shahnam Arshi , Volume 2, Issue 3 (Autumn 2002)
Abstract
Background & Objective: Control of TB is regarded as a health priority program in underdeveloped countries, and Iran in no exception in this regard. Annual risk of infection (ARI) is an important index in this program. This index shows the percentage of people in a society who have been contaminated, and consequently their skin test has changed from negative to positive. In this study, Tuberculin skin test is conducted on Ardabil primary school students and its changes, if any, after one year have been regarded using renewed skin test in order to show ARI in this age group. Methods: 780 students were selected from among 17 primary schools using simple random sampling method. They were 8-14 years old, (mean 9.92). Tuberculin skin test was performed on all subjects two times with a one- year interval (May 1998-1999). The test results were recorded and analyzed. In this study PPD over 10 mm was considered positive. Results: In 55 (7.1%) students the test was positive in both tests, and in 33 (4.2%) students, PPD changed from negative (first time) to positive (second time) and in 78 subjects the results were doubtful. 765 students (98.1%) had history of BCG vaccination. Conclusions: This study indicated that, firstly, over the time, BCG has little effect on Tuberculin skin test, and secondly, the high rate of tuberculin skin test changes from negative (first time) to positive (second time) represents probability of severe contamination in this area.
Ali Majidpour, Yosof Hamidzadeh Arbaby, Nateg Abbasgholizadeh , Esmaeil Salehy, Volume 5, Issue 3 (Autumn 2005)
Abstract
Background & Objectives: Authorities and scholars believe that determining cigarette smoking status of individuals and groups and finding reasons of smoking plays an important role in manipulating and facing with this dirty habit. The present study is an attempt to determine the rate of prevalence and the causes inducing smoking among students of Ardabil university of medical sciences. Methods:This survey was a descriptive and cross-sectional study. Of 1300 medical sciences students, 1106 ones volunteered to participate in this study. The data were collected using a questionnaire and were analyzed in SPSS. Results: 154 students (13.9%) had experienced cigarette smoking previously and 82 students (7.2%) were already a smoker. Intimacy with smokers, self-gratification and acquiring enjoyment, educational failures, vague future career and smoking for entertainment were the most important factors inducing smoking. Conclusion: Cigarette smoking prevalence rate in this study was relatively low in comparison with similar studies. However, regarding the critical social status of these students, some serious measures should be taken to inform them about the dangers of smoking and at the some time some facilities should be provided for students, which can be of great help in this regard.
Afshar Tamook, Farhad Salehzadeh, Naiere Aminisani , Goubin Moghaddam Yeganeh, Volume 5, Issue 4 (Winter 2005)
Abstract
Background & Objectives: The incidence of neonatal hyperbilirubinemia disease is noticeable. Studying neonatal hyperbilirubinemia and its related factors is a step to reduce its incidence and remarkable treatment expenses. On the other hand, due to lack of on time recognition and suitable treatments, this disease can cause irreversible neuro-cerebral complications. This study was conducted to examine the etiology of neonatal hyperbilirubinemia and its associated signs, in order to provide on time and suitable treatment measures.
Methods: This cross-sectional study was performed in 2003 on neonates affected by neonatal hyperbilirubinemia in Ardabil Sabalan hospital. The data obtained from their conditions, clinical examination, and measurment of total and direct bilirubine was used to fill out a questionnaire. The data were analyzed by SPSS software (release 10).
Results: From among 132 neonates affected by neonatal hyperbilirubinemia, 50% were male and others were female. 85.6% of these subjects were fullterm births and the rest were premature. 56% of these neonates were born by C/S. 6.8% of hyperbilirubinemia cases, which were considered as “pathologic”, occurred during 24 hours after birth. 72% were related to the second to seventh days of birth, 17.2% to second week of birth, and the rest (4%) had prolonged Icter. 65% of these neonates were the first children of the family. The average time of hospitalization was 4.8 days. 34.8% of these neonates had pathologic signs beside hyperbilirubinemia and had a clinically serious condition. About 23% of these neonates were born by C/S and only 11.8% of them were vaginal births. 46 cases (34.8%) had accompaynig pathologic problems in addition to Icter, among whom 33 children (25%) had evident infection. The causes of hyperbilirubinemia were Crigler-Najjar syndrome in one case, ABO in 11 cases (8.33%) and RH discord in 4 cases (2.8%). Generally speaking the precise cause of 36.89% of Hyperbilirubinemia was determined.
Conclusion: Some factors such as children by C/S, neonatal infection, prematurity, ABO discords and RH discords play an important role in hyperbilirubinemia incidence and severity.
Farhad Salehzadeh, Volume 5, Issue 4 (Winter 2005)
Abstract
The term periodic fever and diseases classified under periodic fever, have particular situation in pediatrics. Periodicity and fever are the hallmark of these syndromes, though there could be other symptoms and signs in each syndrome. On the other hand, most of these syndromes have familial pattern. Since some of these syndromes are common in north-west Iran, the understanding and recognition of these diseases seems to be necessary. Nowadays at least seven periodic fever syndromes have been explained in pediatrics: The first one is FMF and the last and new one is PFAPA. The rest of them are: Hyper IgD, FCAS, Muckel- Wells, CINCA, and TRAPS.
Farhad Salehzadeh , Dina Emami , Aliasghar Zolfeghari , Abbas Yazdanbod , Shahram Habibzadeh , Bahman Bashardoost , Manoochehr Barak , Eiraj Feizy , Hormoz Azimi , Marina Jastan , Jafar Khalafi , Volume 6, Issue 3 (Autumn 2006)
Abstract
Background and Objectives: Familial Mediterranean fever which is the prototype of the hereditary periodic fever syndromes is common in the countries around the Mediterranean Sea. Regarding the geographical position of the northwest of Iran, having Turkish originality and its vicinity to the Mediterranean Sea , the incidence of this disease is significant in Ardabil. The goal of this study was to introduce Familial Mediterranean Fever as a disease with significant outbreak in this area. Methods: This research is a descriptive study which has been done during one year from October 2004 to October 2005. According to the Tel-Hashomer criteria, the patients suffering from Familial Mediterranean Fever were collected from private clinics and pediatric rheumatology clinics records. Then from 112 patients only 74 ones were studied. All of the patients were interviewed and filled out a questionnaire. Results: Familial Mediterranean fever is common among children under 18 (76%) and more common in male than female. Abdominal pain has been the most common complaint (74%) and abdominal pain and fever (95% and 84% respectively) were the main clinical symptoms. The most common period of pain was 12-72 hours and the common recovery (attack free) period was from 1 week to 1 month (63/5%). Majority of the patients had hospital admission for diagnostic work up (85%) and some of them (32%) had been under surgical operation mistakenly. On the whole 92% of the patients had taken medications as a result of wrong diagnosis and 20% had positive familial history. 50% of the patients' parents were first degree relatives and in 59.5% delay in diagnosis was more than 3 years. Conclusion: Results of this study and introduction of this group of patients in a one-year research indicate that: Familial Mediterranean Fever is more common in the Northwest of Iran although physicians are not familiar with that. The common age for manifestation of this disease is under 18 and its presentation after the age of 40 is very rare.
Hasan Anari, Farhad Salehzadeh , Ramin Mirmohammadi , Simin Monshari , Volume 7, Issue 1 (spring 2007)
Abstract
Background & Objective: Developmental dislocation of hip (DDH) as an inherited joint disorder results from external location of the head of femora from the acetabulum.Early diagnosis of this disorder is very important and any delay may result in long life handicap. Nowadays new methods such as sonogrphy are recommended in diagnosis of this disorder. Radiology and physical examination are traditional methods. This study was designed to evaluate the results of sonographical, radiological and physical examination findings in 100 infants who were suspected to have DDH. Methods: This analytical-cross sectional study was done in private clinics from June 2004 to June 2005. The one hundred infants who were suspected to have DDH in physical examination entered the study. A questionnaire was completed for each infant, and then sonography and radioghray have been done for them. The data were collected and analyzed by statistical programs. Results: Among 100 infants 48% were female and 52% male, with mean age 42±7.23.In 44% they were the firstborn.58% had normal delivery and 42% cesarean section. 15% had breech presentation. In 28% radiological and 56% sonographical methods showed findings in favor of DDH. Conclusion: This study showed, simultaneous physical and sonographic examination especially during the first month result in early and precise diagnosis of DDH, on the other hand it does not have radiologic side effects.
Hadi Sadeghi , Saleh Rohollahi , Volume 7, Issue 1 (spring 2007)
Abstract
Background & Objectives: Ardabil city with population of about 340386 is still supplied with groundwater resource for drinking. That should have optimum quality standards in different aspects. If a drinking water physicochemical parameters levels is higher than the permitted recommended levels, they may create irreversible damages. This study intended to determine the physicochemical parameters of drinking water in Ardabil in the year 2004. Methods: This is a descriptive cross-sectional study that has been done on 35 samples of Ardabil city drinking water resources in 2004. Most of the physicochemical parameters due to their importance and present limitations or even through technical restriction in water and wastewater laboratory of Ardabil health care centers were chemically analyzed and the results were statistically analyzed and compared by 1053 country standard methods. Results: According to the results of analyzed samples and their comparison with their related standard, it is estimated that So4, Po4 and total hardness of samples were %9, %71 & %41 respectively more than maximum acceptable level. Fluorine of samples was %57 less than the minimum recommended fluorine. Conclusion: Due to the lack of consistency in the values of So4, Po4, fluorine and total hardness of testing samples with the related standards, programming for obviation of the problems seems essential.
Vahed Alaei, Farhad Salehzadeh, Volume 8, Issue 3 (Autumn 2008)
Abstract
Background & Objectives: Identifying urinary tract infection, especially in infants and young children is very important from the viewpoint of start of treatment, and if it is delayed probably the children are affected by risk of failure to thrive, sepsis and chronic problems such as renal scars, increase of arterial blood pressure and renal failure. Increasing usage of antibiotics, result in AB resistance microorganism. This study was done because of bacterial differences in resistance in different regions and the change in the rate of resistance in UTI. Methods:This research is a descriptive-prospective study 510 children under the age of 12 as the study samples were chosen. These children were referred to clinics of Ardabil Aliasghar and Sabalan Hospitals in 2006. Their urine culture colony was above 100000. Data gathering was accomplished by their parents through questionnaire and interview's then gathered Data were analyzed by computer software programs of the SPSS and Excel. Results:Findings of research showed that symptoms of malodorous urine (54.7%) in children under 12 months, dysuria (58.9%) in 1-3 years age groups, frequency (54.8%) in 3-6 years old groups dysuria (49.2%) in 6 years old groups and above were most prevalent symptoms which were reported. From the viewpoint of gender differences UTI in girls was 4 times more than that of boys that this difference in 1-3 years old groups was highest range (4.8 to 1). About antibiotical resistancy of microorganisms, Ampicillin with 78.9% resistance, Co-trimoxazole with 66%, Cephalexin with 62.8% and cephazoline with 33.3% were most resistant antibiotics respectively. E-coli resistance against gentamicine was 15.8%, that compared with other microorganisms is very high and its resistance against ceftriaxon was 8%, which is low compared with other microorganisms. Conclusion: Findings of research showed the variety of UTI symptoms in children and also showed that drug resistancy against Ampicillin, Co-trimoxazole, Cephalexin and cephazoline is high.
Rezvan Zabihollahi , Maryam Nourmohammadi , Azar Farhang Esfahani, Rohollah Vahabpour, Seiyed Mahdi Sadat , Mohammad Reza Aghasadeghi , Mansour Salehi , Seiyed Davar Siadat , Volume 12, Issue 1 (spring 2012)
Abstract
Background & Objectives : Several studies have been conducted to explore anti-HIV drugs. Discovery and study of novel anti-HIV-1 compounds need live viruses and has serious biosafety concerns. In this research we reported a novel and safe system for assaying the cytopathic effects of HIV by using single cycle replicable (SCR) HIV-1 virions. Methods: To produce the SCR HIV-1 virions, pMD2G, pmzNL4-3 and pSPAX2 plasmids were co-transfected into HEK293T cells. Different amount of SCR virions were used to infect target cells (MT-2). Within the infected cells, the number of formed syncytia was counted under the light microscopy. The lethal effects of the SCR HIV virions were measured using XTT proliferation assay. Results: Formation of syncytia among SCR HIV infected cells was detectable 24 hours after infection. Highest amount of syncytia was seen 72 hours after infection. Increase in the amount of virions caused increasing of syncytia and the cytopathic effects of SCR HIV-1. Infection with more than 1600ng P24 SCR HIV decreased the syncytium formation and viability of all cells. The calculated IC50 (50 percent inhibitory capacity) for nevirapine and BMS806 using this method was 50nM and 30nM, respectively. Conclusion: SCR HIV-1 virions are replicable only for one cycle. Using these virions can improve the safety of HIV researches. Herein, we optimized the assaying of HIV induced cytopathic effects by using SCR HIV-1 (NL4-3) virions. The accuracy of this method was accepted by quantifying the anti-HIV-1 effects of nevirapine and BMS806 by (SCR) HIV-1 virions.
Ali Niapour, Zahra Taghipour, Sahar Kiani, Fereshteh Karamali, Nazila Niapour, Mohammad Mehdi Mir Hosseini, Mohammad Reza Piri, Hosein Salehi, Noruz Najafzadeh, Mohammad Hosein Nasr-Esfahani , Volume 13, Issue 4 (Winter 2013)
Abstract
Objective: Spinal cord injury (SCI) has become an especially challenging target in experimental neuroscience. Approach into the spinal cord is the interface among all different types of spinal cord injury modeling. The lower thoracic spinal cord has generated special interest due to the lower limbs’ spinal pattern generator position and presence of relative scales for behavioral assessment. However, a clear method with which to approach the thoracic spinal cord has yet to be determined. Methods : A total of 20 animals were subjected to this study. Following induction of anesthesia, the 10th thoracic vertebra were positioned, and muscles were retracted. Using the high speed rotary, the vertebral lamina were carefully thinned. As a final point, the reduced lamina was meticulously removed away to expose underlying spinal cord. Loco motor behavioral test (BBB) was implemented before and after surgery procedure. Results: This manuscript has presented the stepwise method to expose rat thoracic spinal cord. Whole procedure took less than an hour. Animals acquired complete BBB loco motor rating score before and after surgery indicating the safety of procedure. Conclusion: This article introduces simple and practical approach for the rat lower thoracic spine. The anatomical orientation, anesthesia , postoperative management , and common problems are discussed .
Farhad Salehzadeh, Morteza Eslami, Volume 16, Issue 1 (spring 2016)
Abstract
Background & objectives: FMF is an auto-inflammatory and hereditary periodic disorder. The symptoms can occur in more than 80% during the first decade of life. With regard to high prevalence of FMF in northwest of Iran, this study was conducted to introduce especial features of FMF in this area.
Methods: This is a descriptive study performed on 403 patients with diagnosis of FMF according to the Tel-Hashomer criteria. Information obtained from patients' file and entered in the questionnaire. Data analyzed by SPSS v20 using simple descriptive statistical analysis.
Results: In this study 228 (56.6%) patients were male, and the mean age of patients was 21.03 years. The common symptoms were abdominal pain in 93.3% and fever in 88.1% of patients. Abdominal pain was the main complaint(49.6%), the average duration of pain was 43.3±34.5 hours and the average attack-free period was 36.5±29.6 days. 15.1% of patients had positive family history and 12.7% had history of appendectomy. Delayed diagnosis was more than three years in 52.3% of patients. Genetic analysis has been done in 239 patients in which 21.33% had no mutations, 39.7% were compound heterozygous genotype, 25.52% heterozygote and 13.38% had compound homozygous mutations. The most common mutations were M694V/V726A (10.46%) and the most common alleles were M694V (20.9%) and V726A (12.7%). The M694V-V726A genotype (12.7%) was the most common combined mutations in male and the common mutations in female was M694V/M694V (10.4%). Among the patients with abdominal pain M694V/V726A (12.5%) was more common. The genotypes of M680I/V726A (13.9%), M694/V726A and M694V/R761H (16.7%) and the M694V/M694V (33.3%) had the common mutations in patients with fever, chest pain and joint symptoms respectively.
Conclusion: First decade is usual age to presentation of FMF. M694V is the most common mutation and M694V-V726A is the common compound heterozygous mutation. MEFV mutations in this study are similar to Arabs results. It seems that clinical criteria still are the best way in diagnosis of FMF in spite of the fact that erysipelas like skin rash is not common as a clinical criteria in this area
Zarife Sohrabi, Hasan Yaghoubi, Behzad Shalchi, Amirsaleh Delara, Parviz Molavi, Volume 17, Issue 2 (summer 2017)
Abstract
Background & objectives: Epilepsy is a chronic neurological disorder that disrupts normal brain activity due to abnormal electrical discharge of brain cells. Mood swings, depression and anxiety are the common complications in epilepsy. The aim of the present study was to compare alexithymia in patients with Tonic-clonic epilepsy , Juvenile Myoclonic Epilepsy (JME), and healthy individuals.
Methods: In this casual-comparative study, sampling was performed by convenience sampling method. The study population consisted of all epileptic patients and the study sample included 134 participants (N= 74 Patients and N= 60 healthy people) individuals aged 18-35 years. Among the patients, 14 were excluded due to lack of selection criteria and 60 samples (N= 29 with myoclonic and N= 31 with Tonic-clonic epilepsy) remained.
Data regarding alexithymia was gathered by Toronto Alexithymia Scale, and analyzed by ANOVA, Scheffé post - hoc test and t-test were performed for independent groups.
Results: Findings showed that the mean of Alexithymia in those with JME, tonic-clonic epilepsy and normal individuals were 64.44, 61.41, and 54.24, respectively. The difference between the two groups with myoclonic and tonic-clonic epilepsy was not statistically significant. However, there was a significant difference found between those with tonic-clonic epilepsy and normal individuals (T= 6.82; p<0.01). In addition, patients with JME and tonic-clonic epilepsy had more difficulty in identifying and describing emotions, and external thought direction compared to normal ones, while no such a difference was observed between the two groups with epilepsy.
Conclusion: Epilepsy is accompanied by reduced processing of emotional information such as alexithymia. Individuals with tonic-clonic and juvenile myoclonic epilepsy are less qualified for recognizing and describing emotional information, and their intellectual orientation is mostly external. Also, since the location of discharge in both types is widespread and similar, no significant difference in alexithymia was observed between the patients with tonic-clonic and myoclonic epilepsy
Farhad Salehzadeh, Afshan Sharghi, Atena Moteyagheni, Saeid Hosseini Asl, Mahsa Mottaghi, Sepehr Sarkhanloo, Volume 19, Issue 1 (spring 2019)
Abstract
Background & objectives: MEFV gene has a major role in Familial Mediterranean Fever (FMF) as an auto-inflammatory disorder. FMF is most often seen in the people of the Mediterranean area. Considering the significant role of the MEFV gene in many rheumatologic diseases and even non-rheumatologic disorders, it is necessary to identify different variations of these mutations in the healthy and normal population of this area.
Methods: 224 healthy people entered into this study. The blood samples were screened for the 12 most common MEFV gene variants according to manufacturer’s instructions. (FMF Strip Assay, Vienna lab, Vienna, Austria)
They filled a questionnaire containing the required information. All patients were initially evaluated for the FMF symptoms and signs in themselves and their first-degree relatives based on clinical criteria. Chi-squared test and t-test were employed for statistical analysis using SPSS ver.24.
Results: Among 224 cases, 113 cases (50.4%) were male, and 111 cases (49.6%) were female. MEFV mutations were detected in 57 patients (25%) of them, 28 cases were male (49.1%) and 29 cases were female (50.9%). The most frequent mutations were E148Q (18.3%, 41cases), followed by P369S (3.1%, 7 cases), V726A (2.2%, 5cases), A744S (1.3%, 3cases), F479L, M694V and R761H (0.8%, each 2 cases), and eventually K695R (0.4%) respectively. Some mutations such as M694I, M680I (G/C), M680I (G/A), I692del were not seen in these samples. There were compound heterozygous mutations of E148Q/P369S, E148Q/V726A, E148Q/P369S, and P369S / F479L in normal population without any findings in favor of FMF.
Conclusion: Twenty-five percent of the normal population of the northwest of Iran carrying a heterozygous variant of the MEFV gene, E148Q (18.3%) as a most common mutation, which can be considered as a normal variant in the healthy population. The presence of M694I, M680I (G/C), M680I (G/A) and I692del mutations in the normal population can be interpreted with cautiously, while particular compound heterozygous mutations can be considered as normal variants.
Mohammad Reza Izadpanah, Ali Saleh Zadeh, Mohammad Zaiefi Zadeh, Mohammad Nik Pasand, Volume 19, Issue 2 (summer 2019)
Abstract
Background & objectives: Breast cancer is one of the most important causes of cancer deaths especially in developing countries. However, some synthetic compounds such as thiosemicarbazone and its metal derivatives can play a very significant role in reducing the death rate from cancer. The aim of this study was to investigate alterations in the expression of BRCA1, p53 and Bcl-2 genes in breast cancer cell line.
Methods: In this study, the effect of pyrazole derivative of thiosemicarbazone (T) as well as T-bound iron oxide magnetite nanoparticles (T/ Fe3o4 np) were investigated on expression changes of Bcl-2, BRCA1 and p53 in MCF-7 cells. For this purpose, the toxicity of the compounds was first measured using the MTT reaction for 24 hr. Then, the mRNA expression levels were evaluated using real-time PCR reaction.
Results: The results showed a high toxicity for the nano-pyrazole derivative of thiosemicarbazone magnetite so that; the fatality rate for treated thiosemicarbazone magnetite derivative cells was much higher than the compound without any nanoparticle. Also, real-time polymerase chain reaction analysis of gene expression indicated a high effect of the derivative on increasing the expression levels of BRCA1 and p53 genes and also decreasing the Bcl-2 gene expression of Bcl-2.
Conclusion: The use of anticancer compounds like thiosemicarbazone which bound to iron oxide nanoparticles would increase the toxicity of the anticancer compounds along with enhancment of delivery of them with high solubility to target tissue.
Somaieh Matin, Yousef Imani Marani, Hossein Salehzadeh, Volume 19, Issue 3 (atumn 2019)
Abstract
Background: Primary sclerosing cholangitis is a rare and progressive cholestatic disease characterized by narrowing of the biliary tract. Despite high levels of ulcerative colitis, early diagnosis of primary sclerosing cholangitis before the diagnosis of inflammatory bowel disease is rarely reported.
Case history: A 28-year-old woman with no history of medical disease visited our clinic because of a mild generalized pruritus. In initial blood laboratory tests with elevated liver enzymes was observed. In Magnetic resonance cholangiopancreatography (MRCP) was introduced primary sclerosing cholangitis to the patient. Therefore, the patient was under colonoscopy and extensive colitis with terminal ileum involvement was detected and the diagnosis of ulcerative colitis was confirmed in the pathology.
Conclusion: Patients diagnosed with primary sclerosing cholangitis should undergo biopsy colonoscopy to rule out inflammatory bowel disease, even if they have no digestive tract symptoms
Iraj Feizi, Somaieh Matin, Hasan Ghobadi, Saeed Hosseininia, Susan Mohammadi Kebar, Farideh Feizi, Hossein Salehzade, Volume 19, Issue 3 (atumn 2019)
Abstract
Background & objectives: Hydatid cyst is a major hygiene problem in Iran. In 80% of cases, only one organ is involved. However, multiorgan involvement has also been rarely reported. But it seems, the prevalence of hydatid cyst with the involvement of more than one organ in Iran is more than other world. The aim of this study was to evaluate the patients with hydatid cyst multi organ involvement in Ardabil province in northwest of Iran.
Methods: In this retrospective study, 34 patients with hydatid cyst with involvement in more than one organ were included. For each patient, a pre-designed questionnaire including name, age, education level, occupation, place of residence, cyst location, clinical manifestations and serologic test results were completed. Patients, who had previously a history of treatment due to hydatid cyst, were excluded from the study.
Results: The mean age of patients was 36.41 years. 61.8% of the patients were housewives and 64% were rural residents. The most commonly involved organs was liver and simultaneous involvement of liver and lung was observed in 38.2% of patients and was more in women. The association of the right lung cyst with the liver cysts was greater than that of the left lung. Only in 43% of the patients, the serologic test was positive for hydatid cyst, and the relationship between serologic test and type of involvement was not significant.
Conclusion: Regarding the high incidence of hydatid cyst involvement in more than one location in our region, in all patients, especially women with liver hydatid cyst, other organs, especially the lungs, should be investigated
Moslem Dahmardeh, Javad Sadeghinezhad, Zahra Tootian, Dr Mojdeh Salehnia, Volume 20, Issue 1 (spring 2020)
Abstract
Background & objectives: Oxaliplatin is a third-generation of platinum drug which is the main therapeutic agent for the treatment of colorectal cancer. Oxaliplatin not only inhibits DNA replication but also transcription and also induces apoptosis or necrosis in cancer cells and rapidly dividing cell lines. Although it is widely used clinically, there is no enough information regarding its effect on ovarian structure. This study was designed to determine morphometric features of 30- and 60-day-old offspring ovaries using precise and unbiased stereological technique following administration of oxaliplatin during perinatal period in mice.
Methods: In the present experimental study, 32 adult female mice were used for experimental (pre-pregnant, pregnant and lactation) and control groups. Mice were placed in separate cages for mating. Control group received 0.2 ml saline intraperitoneally (IP) during 21 days of pre-pregnancy, pregnancy and lactation periods. Experimental groups including pre-pregnancy, pregnancy and lactation groups received 3 mg/kg oxaliplatin thrice in a week IP during 21 days before mating, during pregnancy and lactation periods, respectively. At the 30th and 60th postnatal days (PND), the offspring were euthanized and the ovaries from eight offspring in each group were collected for stereological analysis.
Results: The results showed that the mean volume oocytes of antral and preantral follicles and also the mean volume of antral follicles were decreased in experimental groups in comparison with the control group in 30 PND offspring (p<0.05). In 60 PND, offspring, the total volume of follicles, mean volume of antral follicles and also mean volume of oocyte of antral follicles in experimental groups showed significant decrease in comparison with the control group (p<0.05). Total volume of ovary, cortical volume and mean volume of preantral follicles decreased in pregnant experimental group compared to control group (p<0.05).
Conclusion: The present results demonstrated that oxaliplatin induces adverse effect on the morphometrical features of the ovary following administration during the perinatal period especially in pregnancy time.
Parisa Habibi, Hadi Yousefi, Mehdi Khazaei, Mohammad Zarei, Iraj Salehi, Reza Jamali Delfan, Simin Afshar, Volume 22, Issue 2 (Summer 2022)
Abstract
Background & objectives: Menopause and especially acute menopause due to surgery is associated with many complications in women. The aim of this study was to determine the effects of genistein and regular swimming exercise (alone/or in combination) on pain through a possible mechanism of inflammation and oxidative stress in ovariectomized rats.
Methods: In this study, rats were divided into six groups, including: control, sham, ovariectomy (OVX), ovariectomized with eight weeks of swimming exercise training (OVX.E), ovariectomized with eight weeks of genistein administration (OVX.G), and ovariectomized with eight weeks of combined treatment (OVX.G.E). The effects of genistein and/or exercise were evaluated by examining the pain intensity with tail-flick and formalin tests. The serum levels of tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), total antioxidant capacity (TAC), total oxidant status (TOS), and malondialdehyde (MDA) were also evaluated by ELISA and spectrophotometer.
Results: In the OVX group compared to the control group, tail-flick and formalin tests showed an increase in pain response. Also, a significant increase in the serum levels of IL-1β, TNF-α, MDA, TOS and a decrease in TAC was observed in the OVX group, however, in the OVX.E, OVX.G and especially OVX.E.G groups, pro-inflammatory cytokines and oxidative stress as well as pain responses showed a significant decrease compared to the OVX group.
Conclusion: A combination of genistein and regular swimming exercise was synergistically more effective in reducing acute and chronic pain than using them alone in the postmenopausal period.
Amirsaleh Delara, Rasol Roshan Chesli , Mehriar Nadrmohammadi, Hojjatollah Farahani, Volume 23, Issue 1 (Spring 2023)
Abstract
Background & objectives: The role of spirituality with its different levels in different countries and societies should be considered for investigating the elderly's health. Therefore, it is necessary to evaluate spirituality with a scale specifically designed to measure spirituality. This study investigated the psychometric properties of the Persian version of the Attitudes Related to Spirituality Scale (ARES) in the elderly.
Methods: In this psychometrics study, the study population included all the elderly of Ardabil city, among whom 459 individuals were randomly selected to fill ARES. The data was analyzed by SPSS and Amos Software.
Results: The reliability of ARES was 0.94 by Cronbach's alpha. Its construct validity, calculated by confirmatory factor analysis, showed that the model for measuring attitudes related to spirituality in the elderly had a suitable fit and its one-factor structure was confirmed (RMSEA=0.001, GFI=0.99, CFI=1).
Conclusion: The Persian version of the Attitudes Related to Spirituality has a one-factor structure, and could be used in psychological studies in Iranian samples due to its acceptable necessary validity and reliability.
Pegah Alempoor, Ata Mahmoodpoor, Kamran Shadvar, Ali Asghar Kheiri, Ali Akbar Ghamari, Parviz Saleh, Seied Hadi Saghaleini, Volume 23, Issue 2 (summer 2023)
Abstract
Background & Objectives: Early warning scores are commonly used by hospital care teams to assess patients and diagnose early signs of their deterioration and initiate further treatment. Due to the existing studies and the lack of sufficient evidence for burned patients, we decided to investigate the value of initial NEWS and MEWS in predicting mortality in burned patients.
Methods: After obtaining permission from the Ethics Committee of TUMS, patients who were candidates for hospitalization in the burn wards of Sina Hospital from July to March 1998 were included. All demographic information including age, sex, APACHE Type of discharge, comorbidities, percentage of burns, initial hospitalization in the burn ward, type and time of initiation and duration of antibiotic use, amount of serum and NEWS and MEWS scores for all patients were enrolled immediately after admission to the ward as well as before discharge from the ward. Data were analyzed after registration in SPSS software version 23.
Results: In this study, 80 cases of burned patients were studied with a mean age of 37.94±18.85 years. The initial NEWS and MEWS indices have a good ability to diagnose burned patients (Area=0.79 and 0.78, p<0.001). The best cut- point based on the Youden index, for the initial NEWS and MEWS indices were obtained at 4.50 and 1.50, respectively.
Conclusion: In this study, for the first time, NEWS and MEWS were examined in predicting mortality in burned patients and it was found that these criteria can predict the prognosis of burned patients.
|
|