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Showing 16 results for Maleki
Kazem Sakha, Arman Malekian,
Volume 5, Issue 2 (Summer 2005)
Abstract
Background & Objectives: Regarding the admitted cases at early infancy in Tabriz Pediatrics Medical Center, this research was carried out to determine the role of Hep.B. vaccination in the incidence of ITP. Methods: We studied all patients’ admission files in Tabriz Pediatrics Center from 1993 to 2002. The patients were under 6 months of age and hospitalized with the impression of ITP. There were 25 infants whose ITP was diagnosed upon their clinical findings, blood counts and bone marrow aspiration and confirmed by exclusion of other causes of thrombocytopenia. Hep. B. vaccination has been included in routine immunization in Iran since 1993. In order to compare the incidence rate of ITP before and after 1993, we also sought all ITP patients’ files at the same age group who had been admitted to Tabriz Pediatrics Center during the decade before the beginning of routine Hep.B. vaccination (i.e. 1982 to 1992). Results: This study shows a considerable increase in incidence of ITP in infants under 6 months of age between 1993 asnd 2002 compared to the decade before the beginning of Hep.B.vaccination, which is statistically significant (P=0.0029). Conclusion: Although there is no facility to measure antibodies in Iran and many other countries, and this is one of the reasons for lack of proof for existence of a relationship between vaccination and ITP incidence, the study of cases in two different periods may strengthen the role of Hep.B. vaccination in the occurrence of ITP at early infancy.
Farzaneh Delgoshaie , Farzaneh Maleki , Mohammad Ramezani , Jamshid Yousefy ,
Volume 7, Issue 2 (Summer 2007)
Abstract
Background & Objectives: Resistance Strains of propionibacterium acnes is one of the most growing problems in acne treatment. Azelaic acid, due to the lack of bacterial resistance, could be a useful alternative in topical treatment of acne. The purpose of this study was to investigate the therapeutic effects and side effects of azelaic acid and its comparison with topical clindamycin in the treatment of acne. Methods: This randomized clinical trial study was performed on 100 patients with mild acne. The patients were divided in to two groups. One group was treated with azelaic acid 20% cream and the other one was treated with Dalacin 1% gel twice daily for 4 weeks. Results: Both drugs Produced significant reduction in inflammatory Lesions (for azelaic acid p=0/000 and for clindamycin p=0/000) and noninflammatory lesions (for azelaic acid P=0/001 for clindamycin p=0/033). Comparing the mean of decrease in inflammatory and noninflammatory lesions, there was not a significant difference between two groups (of Papule and Pustule P=0/864 of comedons P=0/239). So the efficacy of these two drugs was same. In comparing the sideffects, erythem was more common in azelaic acid users significantly (p=0/031) and skin dryness was more Common in clindamycin users significantly (p=0/000). Conclusion: Azelaic acid 20% cream as effective as clindamycin gel 1% in the treatment of mild acne.
Farzaneh Maleki , Farzaneh Delgoshaee , Mohammadreza Khakzad , Mohammad Wejdanparast, Behiyeh Zakerian ,
Volume 7, Issue 3 (Autumn 2007)
Abstract
Background & Objectives: :Contact Dermatitis (C.D) is a common condition and 5.4% of population are suffering from it. Main treatments are based on emollients and corticosteroids. and in C.D cellular infiltration are mononuclears chiefly T. helper lymphocytes. The aim of this study was to have a new approach to herbals with new science covering and study the efficacy of alcoholic extracts of 4 herbals: henna, camomile cedar and aloevera in experimental C.D on mice. Methods: In a pilot experimental study first we chose 70 mice (ENMARY race, 50 ± 10gr weight as case group, then we shaved behind of their left ears (at the days 0 and 1) and rubbed DNCB 400 mg/ml on the skin.At the 10th and 14th day the same amount of DNCB again was rubbed on the left ear. On the right ear the same amount of olive oil in Acetone dissolver was rubbed,as control group. Then at the times of 2, 10, 24, 48, 72, 96, 120, 150 hrs after the 2nd contact, the mice were killed with Ether, cutting the ears from base and prepared pathological sections for study of cellular infiltration. In second stage alcoholic extracts of 4 plants were prepared, then herbal cream with 4% concentration in cold cream as pallish cream were made, with considering the results of first stage that showed the maximal infiltration was at 120 hr after contact, the creams were rubbed every 12hr for 7 days on 40 mice in 4 groups that had been sensitized before with DNCB. 2 groups (20 mice) were considered as control (10 mice received no drugs and 10 mice received cold cream). 60 mice were chosen in the second phase and in general 130 mice were investigated. Results: lymphocytic infiltration reduced after 7 days application of drugs for camomile group about 66/7%, cedar 50%, alaevera 12/5% and henna 0% Conclusion: Camomile chammazolen, Inositol had the best response (66/7% severe response) on improvement of contact dermatitis (P=0.02)
Masoud Maleki , Kazem Parivar , Mohammad Nabiyouni , Parichehr Yaghmaei , Mohammad Naji ,
Volume 9, Issue 2 (summer 2009)
Abstract
Background and Objectives:Stem cells are characterized by the ability to renew themselves through mitotic cell division and differentiating into a diverse range of specific cell types. Wharton's jelly is the appropriate source of mesenchymal stem cells (MSCs) that have high differentiation competence. The aim of this study was differentiation of MSCs to lens fiber cells. In differentiation pathway of lens fiber cells, crystallins are expressed . Thus, crystallins can be used as differentiation marker of lens fiber cells. Method: In the current study MSCs were isolated from the mouse umbilical cord. It was minced into 1-2 mm3 fragments and then were incubated with collagenase type IA following pipetting for mechanical isolation of cells. Cell suspension was plated in 25 cm2 culture flasks. Alkaline Phosphatase detection kit was used for staining of undifferentiated UC-MSCs from passage I. In the experimental group MSCs were plated in the maintenance medium supplemented with bovine vitreous body (1:3 v/v) for induction. Protein lysate were prepared from cells on days 10 of induction and were analyzed on polyacrylamide gels and transferred to nitrocellulose membranes. Rat lens extract was used as a positive control. Anti-alpha A, alpha B crystallin, secondary antibody, vectastain ABC- kit (standard) and vector blue alkaline phosphatase substrate kit III were used. Results: Mouse umbilical cord MSCs had alkaline phosphatase activity. Morphological studies and separation of proteins in electrophoresis indicated that experimental group cells might probably differentiated into lens fiber cells. Conclusion: Mouse umbilical cord could be used as an appropriate source for MSCs. MSCs had fibroblast- like morph and experimental group indicated the presence of fiber-like cells that were long, thin, and parallel aligned. Electrophoresis and Western blot analysis showed there was a detectable expression of early developmental marker of lens fiber cells differentiation in experimental group.
Asadollah Asadi , Arash Abdolmaleki, Farhood Najafi,
Volume 13, Issue 1 (spring 2013)
Abstract
Background & Objectives: Polymers as drug carriers are recent advances in drug delivery and led to the new advent field that called polymer treatment. In the present study, the toxic and teratogenic effects of BDP18 were evaluated against chicken embryos as a model. Methods: The BDP18 tri-block copolymer (PLA-PEG2000-PLA) was synthesized. The compound solution was injected in triplicate examination, in the air sac of the eggs, at third day of incubation, and survived fraction of the embryos and Morphological and skeletal changes were recorded . Results: The survived fraction of the embryos depends on the compound concentration. In concentration of 20 mg/ml , 33.3% of the embryos were survived and the LD50 was 10.87 mg/egg . Morphological study of the treated embryos showed no abnormalities in embryos , and skeletal staining showed the deletion of caudal vertebrate in high concentration. Conclusion: The BDP18 copolymer had low toxic and teratogenic effects against the embryos, but it caused the deletion of caudal vertebrate at concentrations above the threshold (10 mg/ml). This polymer can be used as an effective drug -release system in low concentrations .
Saber Zahri, Masoud Maleki, Kamaladdin Hamidi , Seiyeh Mahsa Khatami ,
Volume 13, Issue 1 (spring 2013)
Abstract
Background & Objectives: Stem cells are fundamental supporter of multicellular tissue. They allow blood, bone, gametes, epithelia, nervous system, muscle, and other tissues to be replaced by fresh cells throughout life. In recent years human Wharton’s jelly stem cells (WJSCs) have gained attention. They express a number of surface markers characteristic of mesenchymal stem cells. In this study, human Wharton’s jelly stem cells were isolated using explant method. To show the stemness property of these cells, three CD markers including CD105, CD44 and CD34 were tested. Methods: The umbilical cord samples were collected by Caesarian section at Arta Hospital in Ardabil. Cords were transferred in sterile conditions and stem cells were isolated using explant method. After log phase, cells were passaged then growth characteristics and CD105, CD44 and CD34 markers investigated by RT-PCR. Results: Separation of human Wharton’s jelly stem cells were started after 7 days. WJSCs in culture revealed two distinct cell population named Type 1 and Type 2. RT-PCR results showed that WJSCs were CD105+, CD44+ and CD34-. Conclusion: Human umbilical cord stem cells could be an alternative source instead bone marrow stem cells for cell therapy and tissue engineering. These cells have a fibroblastic appearance. Following the lag phase and into log phase respectively, cells grow easily in culture and retain stemness properties in higher passages.
Abbas Yazdanbod, Afshin Hooshyar, Rasool Nemati, Nasrollah Maleki, Gholamreza Hamidkholgh,
Volume 13, Issue 4 (Winter 2013)
Abstract
Eosinophilic gastroenteritis is a rare disease characterized by focal or diffuse eosinophilic infiltration of the gastrointestinal system. The clinical presentation is depending on the involved area and its extension into the layers of the gastrointestinal tract. We repor ted a case of eosinophilic gastroenteritis in the setting of cryptogenic cirrhosis which initially appeared as a tumoral obstruction of the 3rd portion of duodenum which is relieved by surgical bypass and two years later presented as eosinophilic ascitis. The patient was treated with corticosteroid with full absorption of ascitis.
Mohammadreza Behvarz , Masoud Maleki , Mohammadreza Mashayekhi ,
Volume 14, Issue 4 (winter 2014)
Abstract
Background & objectives: Adult stem cells are undifferentiated cells that replace dead or injured cells. There are adult stem cells in some regions of human tissues and hair follicle is one of the tissues that have adult stem cell source and these cells have an important role in hair life cycle. In this study, we investigated the isolation of hair follicle stem cells (HFSCs) and expression of mesenchymal stem cell markers on the isolated cells. Methods : Human hair follicles obtained from men scalp tissue by micro punch technique. Hair follicles isolated and cultured in culture flasks in DMEM-F12 + FBS. After outgrowth of stem cells from hair bulges, they analyzed by flow cytometry for detection of stem cell markers. Results: 23 to 27 days after isolation and culture of HFSCs in uncoated cell culture flasks, cell surface markers expression studied by flow cytometry. Flow cytometric analysis showed 25.26% Stro-1, 50.85% CD90, 45.24% CD105, 61.20% CD44, 8.20% CD45, 11.86% CD146, 2.72% CD106, 7.21% CD166 and 26.74% CD19 expression in HFSCs. Conclusion: In this study, isolated stem cells significantly expressed some of the mesenchymal stem cell markers higher than other markers. These markers give certain characteristics to HFSCs, and introduce the cells as an alternative option for cell therapy, tissue engineering and regenerative medicine.
Farin Malekifard, Norooz Delirezh, Rahim Hobbenaghi, Hasan Malekinejad,
Volume 18, Issue 2 (summer 2018)
Abstract
Background & objectives: Several studies have shown that pentoxifylline is an antioxidant and anti-inflammatory agent. Pentoxifylline (PTX), has been shown to exert protective effects on autoimmune disorders. The objective of the present study was to examine the effect of pentoxifylline on histopathology of pancreas in diabetic mice.
Methods: Diabetes was induced by multiple injection of low-dose streptozotocin (40 mg/kg/day for 5 consecutive days) in male C57BL/6 mice. After induction of diabetes, mice were treated with pentoxifylline (100 mg/kg/day i.p.) for 21 days. The nitric oxide levels were evaluated in spleen cell culture supernatant. Pancreases were isolated and stained by hematoxylin & eosin (H&E) and Gomori aldehyde fuchsin (GAF).
Results: Pentoxifylline treatment significantly inhibited the production of nitric oxide (p<0.05). In addition, PTX improved the pancreas tissue. It increased the mean diameter of islets and the number of islets and beta cells. (p<0.05).
Conclusion: These findings indicated that pentoxifylline might have a therapeutic effect against the autoimmune destruction of the pancreatic beta-cells during the development of STZ-induced type 1 diabetes in mice.
Shahin Seidi, Mousa Tavassoli, Farnaz Malekifard,
Volume 20, Issue 4 (winter 2021)
Abstract
Background & objectives: Fleas are clinically important parasites for affecting human health. These insects are carriers of some pathogens such as Yersinia pestis, Rickettsia typhi, Q fever, Tularemia and Bartonella henselae which are infectious for humans and animals. The aim of this cross –sectional study was to detection of Rickettsia, Bartonella and Wolbachia pathogens in infected Ctenocephalides canis and Pulex irritans using molecular method in West and Northwest of Iran.
Methods: The present study is a, descriptive, cross-sectional study (prevalence rate=10%, confidence level=95%, error rate=5%) which performed on samples collected from five provinces including Kermanshah, Kurdistan, Azerbaijan Western, Lorestan and Hamedan for 13 months from May 2018 to June 2019. In this study, samples were collected by optical trap, human prey and direct isolation of the sample from the host and identified in the parasitology laboratory using valid diagnostic keys. The prevalence of Rickettsia, Bartonella and Wolbachia in the collected samples was detected using polymerase chain reaction (PCR). Amplification and sequencing of gltA, pap31 and 16SrRNA genes were used for molecular diagnosis of Rickettsia, Bartonella, and Wolbachia respectively.
Results: The collected samples included 918(47.39%) fleas of C.canis and 1019 (52.60%) fleas of P.irritant. The PCR products of each gene was subject to sequencing. In this study, 12.9% , 5.21% and 5.21% of fleas were positive for Wolbachia , Rickettsia and Bartonella, respectively .
Conclusion: Bartonella, Rickettsia and Welbachia are vector borne infectious agent. Due to their high pathogenicity and easily transmission among insect and human, monitoring of insects is essential for the controlling of the infection and preserving the public health in endemic area.
Abbas Sabahi Namini, Arash Abdolmaleki, Sharareh Mirzaee, Mehrdad Sheikhlou, Asadollah Asadi, Ali Shamsazar,
Volume 21, Issue 2 (summer 2021)
Abstract
Background & objectives: Research on intelligent nanomaterials that accelerate the process of nerve regeneration and treatment by different methods such as antioxidant effects, stimulation of nerve cell proliferation, modulation of the immune system and inflammatory factors is great importance. The aim of this study was to prepare cinnamon-coated cerium oxide nanoparticles and evaluate its antioxidant and cytotoxicity effects on PC12 cell line.
Methods: Cinnamon-coated cerium oxide nanoparticles were prepared and their structural properties were evaluated by transmission electron microscopy (TEM) and Fourier transform infrared spectroscopy (FTIR). To evaluate the antioxidant properties of the compounds, free radical trapping methods 2 and 2 diphenyl-1-picryl hydrazyl were used. Cell viability in the presence of compounds was measured by a toxicity test (MTT) [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide]. Data were analyzed by one-way analysis of variance and Tukey's post hoc test.
Results: FTIR spectra and TEM images showed the processing of nanoparticles with an average size of less than 100 nm with cinnamon coating on their surface. Also, the antioxidant capacity of cinnamon-coated cerium oxide nanoparticles was significantly higher (*p<0.038) than extracts and nanoparticles alone at similar concentrations. Evaluation the results of cytotoxicity showed that the lowest toxicity was observed in the cinnamon-coated cerium oxide nanoparticles group.
Conclusion: Results showed higher antioxidant properties and low cytotoxic effects of cinnamon-coated cerium oxide nanoparticles compared to other groups, which leads to better efficacy, proliferation, longer cell survival, its green synthesis and coverage by cinnamon
Leili Aghebati-Maleki, Ali Aghebati-Maleki, Ali Fotouhi, Sanam Nami,
Volume 21, Issue 3 (Autumn 2021)
Abstract
Candida albicans is the most common cause of invasive candidiasis, but in recent years the incidence of infections caused by other species such as Candida Kruzei, Candida glabrata, Candida tropicalis, Candida parapsilosis and Candida lusitania has increased. In the last decade, the treatment methods for invasive candidiasis have changed completely, and a successful treatment depends on the timely start of treatment, the selection of an effective drug, and the lack of resistance of the fungus to that particular drug. On the other hand, the widespread use of immunosuppressive drugs as well as organ transplants has all caused widespread problems in the treatment of invasive candidiasis. Together, these observations highlight a rationale for the immediate development of new immunotherapy methods to enhance antifungal therapy in immunocompromised hosts. The past decade has seen great advances in our understanding of fungal immunobiology, leading to a number of new molecular and cellular immunotherapy methods for invasive fungal infections. Therefore, the aim of this study was to review the common and new antifungal drugs in the treatment of invasive candidiasis and to discuss the role of immunotherapy in better prevention and control of the disease.
Reza Najafi, Asadollah Asadi, Saber Zahri, Arash Abdolmaleki,
Volume 22, Issue 1 (Spring 2022)
Abstract
Background & objectives: Tissue engineering is a growing field to repair and replace the defective function of damaged tissue or organ, and today it is proposed as a new treatment to replace conventional transplant methods. For this purpose, polymeric biomaterials (scaffolds) and living cells are used. The purpose of this study is to fabricate polycaprolactan (PCL) nanoscaffold and load silymarin on the nanoscaffold to check the biocompatibility and proliferation ability of pc12 cells on it.
Methods: In order to prepare polycaprolactan nanoscaffold and load silymarin on it, 7% polycaprolactan solution (dissolved in acetic acid) was mixed with silymarin solution with a concentration of 0.9% (weight percent), and then the scaffold was prepared using electrospinning device. The morphology of the scaffold was evaluated by scanning electron microscope (SEM) and the chemical structure of the scaffold was evaluated by ATR-FTIR spectroscopy. Toxicity of the scaffold and cell survival of PC12 cells were investigated by MTT test and SEM microscope respectively.
Results: Examining the morphology of the scaffold and its chemical structure showed the appropriate porosity of the scaffold and the successful loading of silymarin on the PCL scaffold. The toxicity of the scaffold was investigated 24, 48 and 72 hours after the cultivation of PC12 cells, and the results showed an increase in cell viability and proper attachment of cells on the scaffold.
Conclusion: The results of this research showed that the loading of silymarin on polycaprolactan scaffold increases the proliferation and survival of PC12 cells. Therefore, this scaffold can be a suitable candidate for nerve tissue engineering.
Aida Nahumi, Maryam Peymani, Hussein A Ghanimi, Asadollah Asadi, Arash Abdolmaleki,
Volume 22, Issue 2 (Summer 2022)
Abstract
Background & objectives: One of the functions of RNA editing is to change the RNA sequence without changing the genomic DNA sequence and changing the fate of cellular RNA. Therefore, studying the clinical application of RNA editing for targeted therapies is necessary.
Methods: All articles related to the subject of the study were searched in the Scopus,
PubMed/Medline, ISI Web of Knowledge, and Google Scholar database.
Results: The changes that occur within the RNA editing are A to I base replacement by adenosine deaminase (ADAR) on RNA and C to U replacement by the apolipoprotein B mRNA-editing enzyme, catalytic polypeptide1 (APOBEC1). Recently, the role of RNA editing in human diseases has been reported.
Conclusion: RNA editing can be used as a new strategy to identify new disease biomarkers and more personalized treatments for various diseases.
Sara Zolfalipormaleki, Hossein Gamari Kivi , Ali Rezai Sharif ,
Volume 23, Issue 3 (Autumn 2023)
Abstract
Background: Given the high prevalence of obesity and overweight and the detrimental impact on the mental health of affected people, the objective of this study is to examine the effectiveness of narrative therapy on social physique anxiety in obese and overweight women.
Methods: The current research was a semi-experimental type, with a pre-test, post-test design with a control group. The study population for this research consisted of all obese and overweight women in Tabriz city in 2023. 30 women were selected by the available sampling method and were randomly divided into two groups: the experimental group and the control group. The experimental group received narrative treatment in 8 sessions of 90 minutes, while the control group did not receive any treatment and was placed on the waiting list. At the end of the treatment period, a post-test was performed in both groups. The Social Physique Anxiety Scale by Hart et al. (1989) was used as the measurement tool. The collected data were analyzed using descriptive statistics (mean and standard deviation) and inferential statistics (covariance test with repeated measures) by SPSS-24 software.
Results: In the pre-test, the mean and standard deviation of social physique anxiety in the experimental group were 44.33±2.289, while in the control group, it was 43.67±3.063. In the post-test stage, the values for social physique anxiety in the experimental group were 36.33±3.117, while in the control group, it was 43.80±3.385. The results indicated the effectiveness of narrative therapy (p<0.001). In the follow-up stages, it was found that the effects of narrative therapy are lasting (p<0.001).
Conclusion: According to the results, it can be concluded that narrative can be an effective method for reducing social physique anxiety in obese and overweight women.
Aida Nahumi, Maryam Peymani, Asadollah Asadi, Arash Abdolmaleki, Yasin Panahi, Mohammad Ali Shahmohammadi,
Volume 23, Issue 4 (Winter 2024)
Abstract
Background: Identifying protein interactions is one of the main challenges in the fields of biostructure and molecular biology. Despite extensive progress, the exact patterns of protein-protein interactions are still unknown. The main goal of this study is to computationally evaluate the interactions of fibronectin-1 in the extracellular matrix of decellularized trachea and integrins in adipose tissue stem cells in order to provide the most accurate possible visualization of these interactions and their role in biological processes.
Methods: After decellularization of the sheep trachea through the detergent-enzyme method, histological evaluations and ultrastructure photography of the samples were done by scanning electron microscopy. Also, the simulations of fibronectin1 binding of extracellular matrix protein with integrin αvβ1 and α5β3 of stem cells derived from adipose tissue were investigated, and interaction energy analysis was applied to predict the structure of protein-protein complexes using the algorithms available in HDOCK and ClusPro servers.
Results: The findings indicated the preservation of extracellular matrix components and scaffold ultrastructure. Also, in order to find the most favorable connection states in terms of energy, some of them were reported as stable interactions among the top types of connections. This insight provides a valuable understanding of cell-matrix adhesion, migration, and signaling, with potential implications for therapeutic development.
Conclusion: The prepared scaffolds are ideal for engineering applications for which computational analysis and experimental data have been used for visualization of stable connection states with energy efficiency between fibronectin and integrin. Also, more studies on cell adhesion modeling in connection with tissue engineering science can provide a suitable field for the development of regenerative medicine in further studies.
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