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  Background & Objectives: Cryptosporidium is a coccidian protozoan parasite. This organism is one of the main causes of severe, long-time and life-threatening diarrhea in immunocompromised persons. It is also among the most prevalent diarrheal agents in children. Cryptosporidial epidemics occur after consumption of water which is contaminated by oosit species of cryptosporidium. Water is usually contaminated by animal feces or by drainage of waste water into drinking water resources.

  Methods: In this study, from ten regions 200 water samples were collected, filtered by 1.2 micron papers and then positive samples were identified in terms of cryptosporidium using PCR method. Finally the related species were detected by RFLP method.

  Results: Nested-PCR showed 8 samples were positive for cryptosporidium that according to RFLP of PCR products 5 samples belonged to cryptosporidium andersony, 2 samples belonged to cryptosporidium parvum bovine genotype and 1 sample belonged to cryptosporidium pig genotype.

  Conclusion: Since Cryptoridium andersony and cryptosporidium parvum bovine genotype are the common species in animals and cryptospovidium swiss is seen in wild animals (pigs and boars), it so we conclude that animal reservoirs have the main role in the contamination of related water resources in this region.

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 Background and objectives: During the last years the effects of pulsative electromagnetic fields have been studied in the human and animals nervous system. The using of these fields in today living has caused worriment in safety of human. Stress causes very problems in behavior and function of biological systems. In this survey the effects of electromagnetic fields on stress behaviors of rats have been studied.

 Methods: In this study we aimed to investigate the effects of electromagnetic fields with 700 milli Gauss currency produced by triangle electrical currents with low (10 Hz) and high (110 kHz) frequencies on stress behaviors in 60 adult, male Wistar rats. The rats were divided to two groups of control and test. The animals were exposed to the field for 80 minutes and in order to evaluate the possible effect of electromagnetic fields the Tail pinch (T.P.) test was conducted. The results were analyzed using statistical tests.

  Results: Analyzing of the results of response time and stress behavior by T-test. In the group exposed electromagnetic fields of triangular low waves (10 Hz) for 80 min the results were meaningful (p < 0.05). The response and stressed behavior with this field is decreased very much. The results of two groups after 80 min under this field that have a difference in frequency showed that effects of frequency in behavior was meaningless.

  Conclusion: The results of this study showed that electromagnetic fields produce anti-stress effect in rats. The radio waves (A.W) have higher effects.

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  Background & Objectives: Malassizia furfur (pityrosporum ovale/orbicular) and other related species are ethologic agents of tinea versicolor and pityrosporosis in normal individuals but fungal infections due these yeasts are a major cause of mortality in immunocompromised and cancer patients. Catheter-related fungemia or foliculitis is most common mycoses in immunocompromised cases, but malassezia Spp., has been frequently implicated as the causative agent of peritonitis, septic arthritis, mastitis, and sinusitis and variety ocular infections. In this study we surveyed Pityrosporom ovale in dandruff of patients with leukemia underlying chemotherapy.

  Methods: Over a one year period, 100 scale samples were obtained from 50 patients with leukemia underlying chemotherapy. All samples were stained using Metilin Blue method. In direct microscopic examination, seeing budding yeast cells with certain numbers, (bottle bacillus) on epithelial cells were reported positive sample.

  Results: Pityrosporosis were dtected in %78 patient with Leukemia. Most of patients were range of 21-30 years old (27%), that suffering from increased scale.

  Conclusion: Malassezia fur fur is one of more common noncandidal yeasts causing a variety of fungal infection. This organism is a lipophilic yeast that colonizes superficially in human skin and causes superficial mycoses such as tinea versicolor, rarely catheter– related sepsis, foliculitis and other systemic mycoses. Most reported cases of systemic mycoses due to this yeast have been in neonates or adults with malignancy or immunocompromised patients, who were receiving parenteral lipids via a central vascular catheter, undergo chemotherapy and BMT. As pityrosporosis were positive in over than 82% of studied patients, suggested that for prevention of serious fungal infections and mortality in immunocompromissed patients, it must be considered a suitable anti fungal protocol for these cases such as using shampoo or other drugs containing antifungal agents for treatment of patient underlying chemotherapy.

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 Background & Objectives: Previous studies indicated that morphine consumption during pregnancy could inhibit embryos development. Present study further evaluated the effects of oral morphine consumption on the maternal and fetal portion placenta cells development in Wistar rats.

 Methods: Female Wistar rats (W: 170-200 g) were used in the present study. Morphine group were received morphine (0.05 mg/ml of tap water) after one night coupling with male rats for mating. On 14^th, 17^th days of pregnancy, the pregnant animals were killed with chloroform and the placentas and uterus were removed surgically and fixed in 10 % formalin. The fixed placentas and uterus were stained by H & E method and evaluated for their development. The thickness of layers, as well as number of the cells in both maternal and fetal parts of the placentas was determined by light microscopy and processed using MOTIC software.

 Results: The results indicated that oral consumption of morphine compared to control group, increased the thickness of the layers in maternal portion and also, increased the number of the cells in both maternal and fetal portion of the placenta.

 Conclusion: All together, oral morphine consumption may inhibit placenta cells development and disturb their natural functions. These abnormalities observed in the placenta by opioid addicted pregnancy Wistar rats.

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Background & objectives: Breast cancer is one of the most important cancers in women worldwide. Taxol as a chemotherapeutic agent, is used for treatment of breast cancer.The aim of this study was to investigate alterations in the expression of mir-1246 and mir-224 in four breast cancer cell lines after Taxol treatment with the goal of introducing them as a biochemical marker for determining response or resistance of breast cancer to the Taxol therapy.
Methods: In this in vitro study, four breast cancer cell lines including MCF-7, MDA-MB-231, SKBR-3 and BT-474 were cultured in RPMI1640 medium supplemented with 10% FBS and antibiotics. Then, MTT assay was performed to determine IC50 concentration of Taxol. Cells were treated for 24 hours and then RNA extraction and cDNA synthesis were performed. Alterations in the expression level of mir-1246 and mir-224 were quantitated using qRT- PCR.
Results: After treatment with Taxol, the expression level of mir-1246 was significantly up-regulated in two HER2-overexpressing cell lines, BT-474 (113 fold) and SKBR-3 (1.4 fold), and down-regulated in two HER2-negative cell lines, MCF-7 (45.5 fold) and MDA-MB-231 (7.7 fold). Expression of mir-224 was detected only in two cell lines including SKBR-3 and MDA-MB-231, and was down-regulated after treatment with Taxol (2.1 and 17.2 fold, respectively).
Conclusions: According to the different pattern of alteration in the expression level of mir-1246 in HER2-overexpressing breast cancer cell lines compared to HER2-negative cell lines after treatment with Taxol, this miRNA could be a useful biomarker for responsiveness to Taxol in  different types of HER2-positive and -negative breast cancers.
 

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Considering the pandemic of the COVID-19 disease, the use of various drugs in patients has been investigated. Recently, numerous studies have been done on the effectiveness of drugs which used to treat the underlying disease such as congestive heart failure, hypertension, as well as coronary artery disease in various countries, because the patients with underlying conditions are more likely to develop COVID-19 disease.
Two classes of the most commonly used drugs in these underlying diseases are angiotensin converting enzyme inhibitor and angiotensin-receptor-blocking drugs. Because the two classes of drugs that mentioned above increase the levels of enzyme-converting enzyme receptor-2, it has been hypothesized that the initiation or continuation of such drugs will play some roles in initiation, progression or acceleration of the COVID-19 disease. In the case of a COVID-19 pandemic, there are some questions; which method should be chosen? start, continue or stop of the two classes of drugs as well as which one should be chosen to have a lower risk in patients suffering from COVID-19?. Accordingly, the studies reported from different countries which conducted with the aim of investigating this assumption, was reviewed in this article. As a result, all of those studies have announced this common result that the start-up order for these two groups of drugs in patients with COVID-19, who have underlying cardiovascular disease, should be treated in the same way as other patients, based on valid and accepted current guidelines. Furthermore, do not discontinue these medications if the patient has taken any of them before having COVID-19 disease.

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Background & objectives: Low birth weight is considered one of the most serious health problems in the world that puts tremendous pressure on the health care system and family. The aim of this study was to investigate the relationship between some maternal risk factors and low birth weight.
Methods: The present study was a cross- sectional study performed on 264 cases. The data gathering tool was a checklist with two parts to compare two groups in this research. The first part was related to the infant's characteristics (sex, weight, and gestational age of the newborn), and the second part related to maternal characteristics. Data were analyzed using SPSS software. Inferential statistics including Mann–Whitney u, chi-square, fisher exact test and logistic regression were used to investigate the relationship between the variables.
Results: In this study, the level of education was lower in mothers with term low birth weight neonates (p=0.024), also normal delivery was reported more frequently among mothers with low birth weight infants (p=0.0001). Also, the mean BMI and the weight at the beginning of pregnancy and overweight in mothers with term neonates and normal weight were greater (p=0.0001, in all). The first minute Apgar score and the fifth minute Apgar score were lower in mothers with low-birth term infants (p=0.002 and p=0.0001). Also distinguished that the gestational age (week) was lower in mothers with low-birth term infants (p=0.0001).
Conclusion: According to the results, increasing progress the pregnancy per week makes it possible to increase the normal weight of newborns by 3.11%.