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Showing 2 results for Kalarestaghi
Sh Heydari Tajaddod, N Najafzadeh, M Mahdavi Rad, H Sheikhkanloui Milan, H Kalarestaghi, V Nejati,
Volume 15, Issue 2 (summer 2015)
Abstract
Background & objectives: Hair follicle stem cells (HFSCs) are multipotent and various types of HFSCs were introduced. HFSCs separation using cell surface markers is one of the interesting strategies in the replacement of old methods. In this study, we used magnetic activating cell sorting (MACS) to separate HFSCs.
Methods: In this study, HFSCs were isolated from Balb/c mice and dissected under an invert microscopy, and bulge area isolated and the bulge cells cultivated about 14 days. The CD34 positive cells isolated using CD34 monoclonal antibody and magnetic activated cell sorting system (MACS), then the cells incubated in DMEM/F12 and 10% FBS. The CD34 positive cells counted using a neubauer slide and evaluated under a fluorescent microscopy.
Results: Here, we isolated CD34 positive cells using MACS and 12±1. 04% of HFSCs were CD34 positive and we found that, CD34 positive cells survived during 7 days cell culture in vitro.
Conclusion: The results show that MACS is useful to increasing density gradient of cells in vitro.
Hossein Kalarestaghi, Mir-Mahdi Hosseini, Ramin Salimnejad,
Volume 24, Issue 3 (Autumn 2024)
Abstract
Background: Liver aging is an important risk factor for chronic liver diseases. Oxidative stress is considered a common pathological mechanism for liver aging. This study aims to investigate caffeic acid's effects against liver injuries in a D-galactose-induced mouse aging model.
Methods: Forty male mice were randomly divided into 5 groups (n=8): 1) control (Con); 2) Sham; 3) caffeic acid (CA), 4) aging (Ag), and 5) aging+ caffeic acid (Ag+CA). The aging model was induced through daily intraperitoneal (i.p) injections of D-galactose (300 mg/kg) for 6 weeks. Caffeic acid (60 mg/kg, i.p.) was injected daily for 6 weeks. One day after the last injection, the mice were anesthetized, blood was withdrawn (for liver enzymes evaluation) and the liver was removed. The histopathological changes in the liver were examined using hematoxylin-eosin staining.
Results: The results showed that D-galactose-induced aging significantly increases the level of liver enzymes (AST, ALT and ALP) as well as liver tissue destruction compared to the control and sham groups (p<0.05). Treatment with caffeic acid in the Ag+CA group significantly decreased the level of liver enzymes and tissue damage index (p<0.05).
Conclusion: The results indicated that caffeic acid can reduce the destructive effects of D-galactose-induced aging in liver tissue.
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