|
|
|
|
|
 |
Search published articles |
 |
|
Showing 2 results for Hamidinejat
Mortaza Nourmohammadi, Hosein Hamidinejat, Mohammadreza Tabandeh, Saad Goraninejad, Somaye Bahrami,
Volume 17, Issue 3 (autumn 2017)
Abstract
Background & objectives: Toxoplasma gondii is a zoonotic obligate intracellular protozoan parasite that infects all warm-blooded animals as well as human worldwide. Determining the parasite genotype in intermediate hosts is crucial in evaluating the role of these types in human infections as wll as in prevention programs. Therefore, this study aimed to identify and detect the genotypes of Toxoplasma gondii in aborted fetuses of ewes in Lorestan province.
Methods: Identification of the parasite was performed on the brain and liver tissues of 142 aborted fetuses using a conventional PCR based on amplification of highly repetitive 529 bp region of the parasite genome. Genotyping of positive samples, which were isolated from the brain and liver, was performed by PCR-RFLP based on SAG2, SAG3 and GRA6 molecular markers.
Results: From a total of 142 samples obtained from brain and fetus, 10 cases (7%) were determined as positive samples based on conventional PCR. The precence of parasite DNA was also confirmed in the liver of 3 positive samples. Evaluation of RFLP pattern of amplified SAG2, SAG3 and GRA6 genes showed the presence of various types of parasites, incuding type I in 3 samples, type II in 2 samples and atypical type in 5 samples.
Conclusion: Isolation of types I, II and atypical type of T. gondii from ewes in Lorestan province suggests the need for greater attention to parasite transmission from livestock to human, particularly in pregnant women and people with weakened immune system.
Morad Beiranvand, Hossein Hamidinejat, Somayeh Bahrami, Mohammad Reza Tabandeh, Meysam Makki,
Volume 24, Issue 2 (Summer 2024)
Abstract
Background: A zoonotic obligate intracellular protozoan parasite, Toxoplasma gondii, infects all warm-blooded animals as well as humans worldwide. Identification of the level of infection in intermediate hosts gives us an important data about understanding the role of this parasite in human health as well as estimating the economic loss in livestock. Therefore, the main aim of this study was the isolation and identification of T. gondii from aborted goat fetuses by PCR in Lorestan province.
Methods: From autumn 2023 to summer 2024, the brain and liver of 100 goat fetuses were examined for T. gondii by PCR based on the amplification of 529 base pair fragments from repetitive regions of the parasite genome. The study was performed in three aborted fetus groups, less than 2 months, 2 to 4 months and more than 4 months.
Results: From a total of 100 examined samples, conventional PCR detected the T. gondii infection in 6 (6%) and 2 of the brain and liver fetuses respectively.
Conclusion: This study shows a notable level of infection in goat fetuses, and as a result, T. gondii should be considered an important agent involved in the abortion of goats in the Lorestan province of Iran. On the other side, it is necessary to pay more attention to the risk of transmission of this parasite from farmed animals to humans, especially pregnant women and people with suppressed immune system.
|
|
|
|
|
|
|
|
|
.png)
.png)
