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Showing 2 results for Hamidi Nokhostin
Nasim Karimzadeh Shushbolagh , Sepideh Mansour Kiaie , Kamaloddin Hamidi Nokhostin ,
Volume 23, Issue 1 (Spring 2023)
Abstract
Background & objectives: Zinc sulfate binds to the R group of some amino acids, such as histidine and cysteine, resulting in protein precipitation. In an ELISA and colorimetric experiments, we determined the optimal concentration of zinc sulfate to precipitate hemoglobin, which in turn affects the level and activity of Caspase 3 in red blood cells.
Methods: Osmotic stress was induced on red blood cells under hypertonic and hypotonic conditions. An isotonic condition was used as a control without osmotic stress. The cells were incubated at 37oC for 15 min and 24 hrs. Different concentrations of zinc sulfate were set up experimentally, stepwise after the lysis of RBC samples with ultrasound waves and removal of cell membranes by centrifugation. Zinc sulfate was allowed to bind to hemoglobin at different time intervals at room temperature. Afterward, hemoglobin was precipitated at various time intervals through centrifugation. The supernatants were then measured by ELISA and colorimetric methods for Caspase 3 level and activity.
Results: The optimal conditions were found to be 6 mM zinc sulfate, 10 min incubation at room temperature to bind zinc sulfate to hemoglobin, and 30 min centrifugation at 3000 rpm to precipitate hemoglobin.
Conclusion: This study showed that zinc sulfate with a concentration of 6 mM precipitates and removes hemoglobin without affecting the level or activity of Caspase 3.
Kamaloddin Hamidi Nokhostin,
Volume 24, Issue 1 (Spring 2024)
Abstract
Enzymes have been used experimentally in the preparation of fermented, food, and medicinal products since mankind started a regular life in the form of early civilizations, without any information about their nature being available. It has been more than a century since scientists have obtained information about the enzymatic reactions and the enzyme structure, and a newer and more practical attitude towards them is obtained day by day. Today, these proteins play roles as biomarkers in the diagnosis, monitoring and treatment of diseases. Enzymes roles as receptor for some hormones and their qualitative and quantitative changes interfering in the onset of some diseases such as diabetes have been known. In addition, they are used as accurate and specific tools in the assay of some biochemical analytes, phenotypic identification of bacteria and biosensor design. The basis of the mechanism of some drugs is enzyme inhibition. Meanwhile, some enzymes are directly used as drugs. The enzymes are applied in some immunoassay techniques, genetics and proteomics laboratory methods. In modern biotechnology, enzymes are used to produce plenty of products. This review article aimed to take a new, updated and comprehensive approach to several roles that enzymes play in various fields from diagnosis, monitoring and treatment of diseases to their application in medical, genetics and proteomics laboratory methods.
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