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Showing 2 results for Gheibi
Hamideh Asghari , Nematollah Gheibi , Kourosh Goodarzvand Chegini, Mahdi Sahmani , Darioush Ilghari ,
Volume 14, Issue 2 (summer 2014)
Abstract
Background & objectives: Calprotectin, S100A8 and S100A9 are involved in important processes including cell signaling and regulation of inflammatory responses. In this study, recombinant expression, purification and structural characterization of S100A8 and S100A9 were accomplished. Methods : In this experimental study, pET15b was used as vector of human S100A8 and S100A9 coding sequences, hosted by E.coli BL21 (DE3). Gene expression and purification attempts were evaluated using SDS-PAGE. Protein purification was accomplished using Ni-NTA resin based on its affinity for His-tag present on recombinant proteins. Tertiary structure of proteins were evaluated using spectrofluorimetry. Results : The subunits were over expressed 3-4 hours following induction at 37 °C. S100A9 was expressed mainly as inclusion body while S100A8 was found to be expressed mainly as a soluble protein. Purification of S100A8 and S100A9 was achieved at 100 mM imidazole. Spectroscopic studies showed that the amino acid tryptophan is in the internal structures and is less exposed to the aqueous environment. Conclusion : In this study, a recombinant S100A8 and S100A9 subunits were expressed and purified and also their structures were confirmed.
Nematollah Gheibi , Javad Shahbazi, Zahra Zarmohammadi , Mahmoud Alipoor Heydari , Eftekhar Kakaeie, Mohammad Sofiabadi ,
Volume 17, Issue 1 (spring 2017)
Abstract
Background & objectives: Propolis is a natural product with powerful antioxidant and therapeutic effects. The aim of this study was to investigate the effect of propolis on passive avoidance memory in adult male mice.
Methods: In this study, 40 adult male mice were divided into 8 groups, including control, sham (solvent) and 3 treatment groups orally treated with 50, 100 and 200 mg/kg of propolis, respectively for two weeks before and one week after treatment. Then, passive avoidance learning and memory were recorded in timescales of 24 and 48 hours, 4 days and a week after shock by the shuttle box. Data were analyzed by ANOVA and Dunnett’s post hoc tests, and p<0.05 was considered significant.
Results: Administration of propolis (50 mg/kg) significantly increased the dark chamber entering time at intervals of 24 and 48 hours (p<0.001) and at concentrations of 100 and 200 mg/kg in all time periods after the shock (p<0.001).
Conclusion: Oral administrations of propolis can improve learning and memory dose-dependently in adult male mice.
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