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Showing 2 results for Subject: Biotechnology
Faranak Hasanpour, Saber Zahri, Arash Abdolmaleki, Asadolah Asadi,
Volume 24, Issue 3 (10-2024)
Abstract
Background: Tissue engineering, by designing biological scaffolds and imitating the extracellular environment, helps the growth and proliferation of cells and plays a key role in replacing and repairing damaged tissues. In recent years, the addition of nanoparticles, such as carbon quantum dots, to biological scaffolds has received attention. In this research, the synthesis of polycaprolactone scaffolds containing carbon quantum dots and the investigation of biocompatibility effects and their protection have been discussed.
Methods: Carbon quantum dots were synthesized using the pyrolysis method, and polymer scaffolds containing carbon quantum dots were prepared by the electrospinning method. The physical and chemical properties of the scaffold were evaluated by scanning electron microscopy and FTIR spectroscopy. The scaffolds' biocompatibility and antioxidant properties were measured by the MTT method.
Results: Examination of the morphology and chemical showed the appropriate porosity of the scaffold containing carbon quantum dots. The MTT assay significantly enhanced stem cell viability on scaffolds incorporating carbon quantum dots. Furthermore, these scaffolds exhibited a significant protective effect against oxidative stress.
Conclusion: This study showed that the polycaprolactone scaffold containing carbon quantum dots, with high biocompatibility and suitable antioxidant properties, provides an effective substrate for tissue engineering and cell protection under oxidative stress conditions.
Nasim Hajighahramani, Fatemeh Ghasempour,
Volume 25, Issue 2 (7-2025)
Abstract
Background: Breast cancer is a leading cause of cancer-related mortality in women, and finding effective treatments is essential. Among different cancer immunotherapy strategies, vaccines play a prominent role. This study aimed to design a multi-epitope peptide vaccine based on Virus-Like Particles (VLP) against breast cancer using computational methods.
Methods: Antigen sequences of HER2, MUC1, Alpha lactalbumin, Mammaglobin-A and the MS2 adjuvant were retrieved. T-helper (HTL) and cytotoxic T lymphocyte (CTL) inducing epitopes were identified using servers such as IEDB, and their antigenicity and allergenicity were analyzed. Molecular docking was performed using HPEPDOCK between selected epitopes and corresponding MHC molecules. The best selected epitopes and adjuvants were connected by linkers. The designed vaccine’s properties, including allergenicity, antigenicity, solubility, and physicochemical properties were assessed. B-cells and IFN-γ inducing epitopes were identified. Finally, the vaccine’s 3D structure was modeled, refined, and validated.
Results: Epitopes from HER2, MUC1, Alpha lactalbumin, and Mammaglobin-A were identified through immunoinformatics analyses and selection of common HLAs in Iran. The 3D structure of the vaccine was designed, optimized, and validated, showing good stability, solubility, and antigenicity.
Conclusion: This study designed a VLP-based subunit vaccine with adjuvant properties that can enhance antigen presentation and induce robust B and T lymphocyte responses. The vaccine is a promising candidate for preventive or therapeutic use against breast cancer, though experimental and clinical studies are necessary to confirm its efficacy.
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