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Showing 6 results for Zoonosis

Hormoz Azimi, Maryam Nasimi, Reza Keikhosravi, Masoud Ghasemi,
Volume 8, Issue 1 (4-2008)
Abstract

Hydatid cyst disease is a zoonosis disease that is common in both humans and animals. Humans acquire echinococcosis by ingesting viable parasite eggs with their food. The hydatid cyst tend to be in the liver (50-70% of patients) or lung (20-30% patients) but may be found in any other organs of the body, including brain, heart, and bones (less than 10%). Symptoms are often absent, and in many cases infection is detected only incidentally by imaging studies.
This paper presents a case of hydatid cyst who admitted with non specific symptoms such as blotting, recurrent constipation and abdominal distention. Abdominal CT of the patient found low density mass in the right side of abdomen (in small bowel mesentery), without involvement
of the liver, kidney, spleen, and pancreas. The patient candidated for resolution of cyst with lapratomic style. And in operative laparatomy a typical hydatid cyst was found and confirmed by pathology and similar reports were not found by the author regarding the literature.
Hosein Alimohammadi, Nasrin Fouladi , Firouz Amani , Mehran Safarzade, Farhad Purfarzi , Effat Mazaheri ,
Volume 8, Issue 4 (12-2008)
Abstract

 Background & Objectives: Toxoplasmosis is an important zoonosis parasitical disease. Infection with Toxoplasma gondii in transmission of parasite to fetus or its reactivation among immune-compromised persons can lead to server clinical symptoms. The aim of study was to determine immune level of Toxoplasmosis among women who were to get married and have referred to Health Center of Ardabil.

  Methods: In this study 272 blood samples of women who had referred to health center of Ardabil in 2007 were collected. Samples were tested with ELISA method to detect Anti-Toxoplasma IgM and IgG Antibodies.

  Results: The tests showed that 42.3% of women were IgG Positive, 57.2% were IgG negative and 2.6% women were IgM positive and also IgG positive. They have ( IgG positive women) 11.3% family history of abortion, 96.5% eating raw vegetables, 92.2% eating half cooked meat, 89.6% not washing the vegetables with disinfectants, 29.6% animal contact, 57.4% contact with raw meat and 94.8% used piped water.

  Conclusion: Since 57.7% of women who were to marry in Ardabil city were negative from the view point of Toxoplasmosis, health education for elimination of risk factors especially within pregnancy period seems mandatory.


Hafez Mirzanejad-Asl, Majid Fasihi Harandi,
Volume 9, Issue 4 (12-2009)
Abstract

 Background and objective: Cystic Echinococcosis (CE) is a cosmopolitan and prevalent zoonosis in the world. It caused by larva of Echinococcus genusspecies. CE is medically and economically one of the important parasitic zoonoses in Iran especially Moghan Plain. So far no survey was conducted to determine the rate of CE in this region.

 Method: The subjects of this descriptive- analytic study were people of the Moghan plain. The prevalence of the disease was determined with serologic examination using ELISA test. ELISA was carried out using HCF-Ag that obtained from a diseased sheep. Household information was recorded in questionnaires before collecting serum samples. The results were analyzed by SPSS using statistical tests.

 Result: Out of 2008 samples, 1267 were female and 734 were male. At all 9.2 % (184) of sera were found to be positive.

 Conclusion: This survey determined more prevalence of Cystic Echinococcosis in women (%10) than men (%7.9) and infection is more in places that keep much Dogs.


Hamidreza Honarmand , Mohammadreza Khoramizadeh, Saeid Eshraghi,
Volume 9, Issue 4 (12-2009)
Abstract

  Background and objectives: Leptospirosis is a very common zoonosis in the world. Early diagnosis of leptospirosis is critical because just early treatment will be effective. It's culture is very slow and serological assays are not applicable because of lack of antibodies in the first week of the disease, therefore PCR is the only option for the early diagnosis. In this study, sensitivity and accuracy of a non-quantitative conventional PCR for diagnosis of leptospirose in first week sera of patients, is evaluated

  Methods : Seventy first week sera sample of patients with clinical diagnosis of leptospirosis which were negative in MAT but positive for the second time after one weeks (seroconversion) were selected and studied.

  Results : We observed twenty four positive sera in PCR test. Sensitivity of the test was 74.5% and accuracy was 100 bacteria /ml.

  Conclusion : Result of our study shows that PCR is the only choice for the early diagnosis of Leptospirosis while other assays are not applicable but its sensitivity is low.


Ali Pezeshki, Mostafa Rezaeian , Mitra Zarebavani,
Volume 13, Issue 2 (6-2013)
Abstract

  Background & Objectives: Giardia duodenalis is the most common intestinal parasite with cosmopolitan distribution. This parasite has been found in the intestine of humans and other mammalian hosts including cats, dogs, cattle, sheep, deer, pigs and muskrats. It is postulated that animals maybe reservoir for human infection and viceversa. In present study, the possible genetic similarity between cat and humans Giardia and its probable zoonosis were investigated.

  Methods: Direct examination and formalin-ether concentration techniques were performed on stray and semi stray cat fecal specimens. Gradient sucrose method was applied for collection and purification of cysts and DNA extraction was performed by phenol-chloroform and CTAB (Cetyl trimethyl ammonium bromid ( methods. DNA of cysts could hardly be extracted after repeated freezing and thawing. Polymerase chain reaction (PCR) was performed for DNA amplification. In this study triosephosphate isomerase (tpi) gene was selected as a molecular marker. Two sets of primers (PM 290 and PM 924) were considered. Two restriction enzymes RsaI and AvaI were also used to determine restriction fragment length polymorphism (RFLP) for PCR fragments amplified by both primer sets.

  Results: Ten samples were positive for Giardia cysts which were examined for molecular investigation. Four cat isolates were amplified by PM 290. PCR-RFLP patterns were found to be similar to human isolates AC≠AF 069556 (subgroup of AC≠U 57897) with possibility of cross-transmission.

  Conclusion: Therefore the similarity of genomic characters of isolates of cat and human Giardia implies possibility of zoonosis and transmission of these protozoa from cat to human and vice versa.


Hafez Mirzanejad-Asl ,
Volume 17, Issue 3 (10-2017)
Abstract

Background & objectives: Alveolar and cystic echinococcosis (CE and AE) are caused by the larval stages of Echinococcus multilocularis and Echinococcus granolosus, respectively. This study was conducted to determine the spread of human AE and CE diseases among the tribes and livestock breeders and farmers in the Moghan plain. The prevalence of infection (CE and AE) significantly increases with a rise in age.
Methods: In this study, using ELISA and HCF-Ag (raw liquid antigen of hydatid cyst) was used for the first time to perform screening, and then the prevalence of cystic echinococcosis and alveolar echinococcosis was evaluated by specific anti-genes Ag-5 and EM2+, respectively. A total of 2003 serum samples were collected randomly from normal populations of five different areas of Moghan plain. In the first stage, serum screening was tested using an enzyme-linked immunosorbent assay and HCF-Ag. After that, the serology of all sera was evaluated by ELISA and specific antigens for alveolar and cystic Echinococcus.
Results: From 2003 samples, 24 serum samples were (1.19%) AE-positive. Infection was higher in men than women (2.1% vs. 0.6%). The age group of 5-19years had the lowest infection rate and the age group 40- 59 years had the highest infection rate (2%). In the case of cystic ecinococcosis, 164 serum samples were positive for Ag-5 specific antigen, i.e. 7.6% infection, and 156 serum samples were positive for Ag-B-specific antigen.
Conclusion: The findings showed that the highest infection rate was found in the Boran and Eivazlou (Palanglou) regions of Moghan plain and the lowest level of human infection was reported in Parsabad region (Nadar-kandi and Agh-ghabagh). In areas with higher contamination, the risk factor associated with CE and AE were the type of water used, the method of washing edible and wild vegetables, occupation and having or not having knowledge of hydatid disease. The infection of Moghan region dogs is one of the main indicators and potential risk factors for human infection.

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مجله دانشگاه علوم پزشکی اردبیل Journal of Ardabil University of Medical Sciences
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