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Showing 11 results for Sperm

Mahzad Sadaghiani , Hale Ayatollahi , Fariba ; Nanbakhsh , Atoosa Mojahadieh,
Volume 6, Issue 2 (6-2006)

  Background & Objectives: Infertility affects approximately 10-15% of reproductive age couples. Intra Uterin Insemination (IUI) is a primary, simple and effective method of infertility treatment in ovulatory disorders, unexplained infertility, cervical factors and some cases of male factor. In this research, the success rate of IUI with regard to different causes of infertility was studied in Kowsar IVF centre.

  Methods: In this retrospective study, 404 infertile couples were studied using a questionnaire that included the demographic data, duration of infertility , sperm count and mobility, follicular diameter and count, situation of uterus and fallopian tubes and pregnancy results. The data were analyzed with SPSS.

  Results: Pregnancy rate was 11.1% for patients with ovulatory factors and 7.1% for male factors. There were no significant differences in sperm count and diameter of follicles in pregnant and nonpregnant women.

  Conclusion: With regard to different causes of infertility the best treatment results were achieved by IUI in ovarian factor. Patients over 35-40 did not have a good prognosis in COH-IUI cycles. Other methods of ART are recommended in such cases.

Mokhtar Mokhtari , Mehrdad Shariatie , Nazanin Tadayon ,
Volume 7, Issue 1 (4-2007)

 Background & Objectives: Spironolactone is a diuretic and antiandrogenic drug and is used in the treatment of hypertention 'secondary hyprealdosteronism congestive heart failure' cirrhosis of the liver, nephrotic syndrome 'androgenic alopecia' gynecomastia and hirsutism. In this research, the effects of spironolactone on the serum LH, FSH, testosterone, dihydrotestosterone, and changes in body weight and testicular tissue in adult male rats, were studied.

 Methods: For this purpose 190 10 g male wistar rats (n=40) were randomly divided into the following grups: control, sham operated (received water) and 25, 50, 100 mg/kg oral spironolactone treated groups. After 14 days body weight and testis weight under laboratory methods, were measured and blood samples were taken from heart and used for the measuring of LH/FSH/testosterone and dihydrotestosterone and then the rates' testes, in order to evaluate the histological changes, were removed and weighed and after obtaining tissue section and staining through HE, they were studied.

 Results: Serum LH level showed a significant increase and testosterone and dihydrotestosterone levels showed asignigicant decrease in 100mg/kg spironolactone treated group ( p 0.05 ) and there was no significant difference among serum FSH level, body weight and testicular weight as compared to control group.

 Conclusion: It can be concluded that oral administration of spironolactone maximum dose for 14 days could increase serum LHlevel and decrease testosterone and dihydrotestosterone levels.

Kamaladdin Hassanzadeh , Parisa Yavari Kia , Vahid Emdady, Seyedkazem Maden ,
Volume 10, Issue 3 (9-2010)

 Background and Objectives: Three major semen parameters that involves on male infertility are sperm count, motility and morphology. In 50% of couples referring to physicians, because of infertility, male factor, solely or accompanying with female factor is responsible of infertility. Some of drugs are used to improve the semen parameters. The goal of this study was to study the effect of pentoxifylline on semen parameters (count, motility, morphology, volume of semen).

 Methods: Sixty one patients with impairment in semen parameters were selected and semen samples requested from each participant for analysis before treatment. Then patients were treated with pentoxifyllin, 400 mg three times a day, for three months. After that we took semen specimens again and analyzed them.

 Results: Mean total sperm count in case group before treatment with PF was (17 millions) and after treatment with PF reached to (21 millions). In 68.9% of patient, Sperm count increased (p<0.001) Mean motile sperm percent in case group before treatment with PF was 19.42% and After treatment with PF reached to 28.78% and 88.52% of patient this parameter increased (p<0.001). Parameter motility showed the most important after treatment. Mean normal morphology of sperm percent in case group before treatment with PF was 24%and after treatment with PF reached to 26.39% and in 32.8% of patient this parameter increased (p<0.001).Mean semen volume in this group before treament with PF was 1.95ml and after treament with PF reached to 1.93 ml. There was no significant variation in semen volume after treament with PF. (p=0.321)

 Conclusion: Results show that Pentoxifylline has significant efficacy for increasing sperm count, motility and morphology correction (specially for sperm motility). Considering that oral PF is safe and cheap, with easy application, we can use it for improving semen parameter’s quality before performance ART.

Masoumeh Hajishafiha, Shaker Salari Lac , Minoo Khairi Tabar , Siyamak Naji, Mahzad Sadaghiani , Nahideh Asadi ,
Volume 11, Issue 1 (4-2011)

  Background & objectives : Today there is a significant progress in the treatment of female infertility but there is no main improvement for the rate of implantation and live birth. This is because of non-implantation and early abortion that lead to decrease the rate of live birth. Genital infections such as bacterial vaginosis are common cause of this problem.

  Distinction and treatment of bacterial vaginosis is easy and non-expensive. Treatment of bacterial vaginosis could results in improving the rate of implantation and then live birth.

  Methods: We considered 209 infertile women treated with ICSI (intra cytoplasmic sperm injection). This study was performed in infertility clinic of KOSAR hospital affiliated to Urmia University of Medical Sciences, Iran.

  Before transferring of embryo, a sample was taken from posterior culdesac secretions by sterile cotton swap and fixed it on lamella. Then bacterial vaginitis was graded by a pathologist. The relationship of bacterial vaginosis with implantation and early abortion was studied. Data were entered into SPSS software and analyzed by t-test and Chi-Square test. p<0.05 is considered statistically significant.

  Results: Bacterial vaginosis was significantly more frequent in patients with tubal and ovulatory disorder (p=0.013). In women undergoing ICSI, bacterial vaginosis was not associated with decreased conception rate (p=0.892) and with increased rate of early pregnancy loss (p=0.44).

  Conclution: Bacterial vaginosis is prevalent in women with infertility. It is also the most important cause of infertility in patients with tubal and ovulatory disorder. Bacterial vaginosis does not affect fertilization rate.

Amirabbas Rashidi, Piraste Norouzi, Hamid Kalalianmoghaddam, Mehdi Khaksari, Mehdi Bagheri,
Volume 16, Issue 1 (4-2016)

Background & objectives: Diabetes mellitus mediated by oxidative stress, creates serious metabolic disorders in testis. Kudzu root with an isoflavonin and saponin contents is often used as antidiabetic and antioxidant. This study aimed at preventing the oxidative effects of diabetes using Kudzu root.

Methods: In this study, 32 male Wistar rats were randomly selected and divided into four groups: control, diabetic and diabetic rats treated with Kudzu 50 and100 mg/kg. Diabetes was induced by intraperitoneal injection of 55mg/kg streptozocin. One week after injection, the rats started to receive Kudzu at the doses of 50 and 100 mg/kg for five weeks by gavage. Testicular damage was examined by using hematoxylin-eosin staining protocol. Hormonal and blood biochemical factors were measured.

Results: The results of this study showed that diabetes causes a high blood sugar levels and reduces the spermatogonia and Sertoli cells with decreased spermatogenesis,sperm count and function. These effects were improved in the treatment groups. Decrease in blood sugar and increase in the number and motility of sperm cells and spermatogonic cells were also observed together with enhanced seminiferous tubule diameter and lower basement membrane thickness.

Conclusion: Kudzu with the ability to reduce blood sugar, improves diabetic-induced testicular damage and can have a therapeutic role in diabetes.v

Somayeh Bahrami, Hossein Najafzadeh, Ali Shahriari, Sara Ahmadi, Mohammadhossein Razi Jalali ,
Volume 16, Issue 4 (1-2017)

Background & objectives: Polyamines such as putrescine, spermidine, and spermine are ubiquitous in all eukaryotic cells and play an essential role in cell division and differentiation. One way of polyamine biosynthesis is done by ornithine decarboxylase (ODC) which catalyzes the transformation of ornithine to putrescine. The aim of the present study was to evaluate the level of putrescine, spermidine and spermine in protoscolices, hydatid fluid and germinal layer and also to evaluate ODC activity.

Methods: In the present study putrescine, spermidine and spermine levels were investigated in germinal layers, hydatid fluids and protoscolices. To evaluate the activity of ODC, protoscolices were incubated with ornithine and changes in polyamines level were assayed. The samples were homogenized and liquid chromatography (HPLC) was used for polyamines measurement.

Results: Based on the results, putrescine was the lowest polyamine and since its level was not increased in protoscolices incubated with ornithine, ODC activity was not detected. Spermidine was the highest polyamine and the results showed that germinal layer contained the highest level of polyamines.

Conclusion: Overall, the results showed that ODC activity was not detected in hydatid cyst and level of polyamines in germinal layers  which contained rapidly proliferating cells was higher than other parts.

Eslam Zabihi, Seeyedeghbal Motavalli Bashi , Pirooz Pourmohammad, Ali Abedi ,
Volume 17, Issue 2 (7-2017)

Background & objectives: Terfezia Boudieri (TB) has been used as a sexual stimulant for men in traditional medicine. TB containing fatty acid, flavonoids, beta-carotene, minerals and antioxidants such as, catechin, therefore, this study aimed to investigate the effects of the hydroalcoholic extract of Terfezia Boudieri on sperm and testosterone levels in male rats.
Methods: In this study, 21 adult male Wistar rats, each weighing approximately 250±20g, were randomly divided into three groups (n=7). The first group (control) with no treatment, the second group (sham group) received normal saline (extract solvent) and the third group, was injected intraperitoneally (IP) with 105 mg / kg of methanolic extract of Terfezia boudieri (TBME) (0.2 ml) for 21 days. The blood samples were collected to determine the concentration of testosterone and  finally, the weight and size  of the testicles and epididymis, the number and the  percentage of sperm moving were evaluated Data were analyzed using ANOVA and Turkey's post- hoc tests.
Results: The results of the present study showed that serum levels of testosterone, body weight of rat, testis weight , sperm count and  sperm motility in the experimental group were significantly increased compared with the control group (p<0.001).
Conclusion: The results of this study showed that TB at 105 mg/kg dosage can increase the levels of testosterone and improve sperm parameters and therefore, TB can be used to treat sexual impotence and infertility in males.
Mina Kiani, Paria Parto ,
Volume 17, Issue 4 (1-2018)

Background & objectives: The present study examined the gonadotoxic effects of ciprofloxacin antibiotics and protective effects of L-carnitine.
Methods: In this study, 20 NMRI mice were divided into four groups: control, ciprofloxacin, L-carnitine, L-carnitine-ciprofloxacin (each group included 5 animals). The control group received normal saline, the treatment group 1 received 12.5 mg/kg ciprofloxacin, the treatment group 2 received 100 mg/kg L- carnitine and the treatment group 3 received 100 mg/kg L- carnitine and 12.5 mg / kg ciprofloxacin simultaneously. All animals were treated by intraperitoneal administration for 15 days. Testis and epididymis were collected to evaluate sperm parameters (sperm count, motility, morphology and viability).
Results: In the ciprofloxacin group, a significant decrease in sperm count, sperm viability, sperm motility, progressive sperm motility and normal sperm as well as a significant increase in rotating sperm motility, sperm without movement, head and tail abnormalities were observed (p≤0.05). A significant increase in sperm count, sperm survival, motility and progressive sperm motility, normal sperm and a significant decrease in rotating sperm motility, sperm without movement and head abnormalities in the L-carnitine-ciprofloxacin group were observed (p≤0.05); however, the reduction in tail abnormalities of sperm was not significant.
Conclusion: L-carnitine improves sperm parameters in the adult mice treated with ciprofloxacin.
Shima Shafiee, Mino Mahmoodi, Siamak Shahidi ,
Volume 17, Issue 4 (1-2018)

Background & objectives: exposure to lead has a wide range side effects on fertility. Vitamin D is one of the most important vitamins required for the body. This study was conducted to determine the effect of vitamin D on spermatogenesis in male rats treated with lead nitrate.
Methods: In this experimental study, 25 adult male Wistar rats (250-300 gr) were randomly divided into 5 groups (n=5). Control group without any treatment, the group receiving lead by gavage and experimental groups receiving lead plus vitamin D at doses of 25, 50 and 75 mg/kg body weight by gavage  for 28 days. At the end of the study, after anesthetizing the rats, blood samples were collected directly from heart and serum levels of testosterone hormone, Follicle-stimulating Hormone (FSH) andLuteinizing Hormone (LH) were measured. Histological studies were performed to count the spermatocyte and examine the diameter of the seminal tube. Data were analyzed by SPSS software using one-way ANOVA, at significance level of p>0.05.
Results: Compared to the control group,  the mean serum testosterone level in the lead group significantly decreased (p<0.001) and the mean LH and FSH serum levels significantly increased (p<0.001) . Also, spermatocytes and seminiferous tubule diameter significantly decreased (p<0.001). Vitamin D consumption reduced the effects of lead intake, and  this effectiveness was completely dose-dependent.
Conclusion: Our data showed that vitamin D has a significant effect on serum testosterone levels and gonadotropins.
Taktam Sadat Vafa , Mojdeh Emadi , Seyed Damoon Sadoughi,
Volume 18, Issue 1 (3-2018)

Background & objectives: Curcumin has antioxidant properties. The aim of this study was to determine the effect of curcumin on bax, bcl-2, antioxidant enzymes and lipid peroxidation of sperm after freezing procedure.
Methods: In this experimental study, semen samples were collected from four mature Holstein bulls, twice a week in eight innings. Semen samples were divided into four groups. Zero (control), 10 (Experimental group one), 20 (Experimental group two) and 30 (Experimental group three) mg/ml of curcumin with diluents were added to the semen samples. After thawing, Bax, Bcl-2 and malondialdehyde levels as well as superoxide dismutase, glutathione peroxidase and catalase enzymes were measured in sperm samples using ELISA.
Results: According to the results, Bcl-2, superoxide dismutase, glutathione peroxidase and catalase levels  in sperm samples treated with 20 and 30 mg/ml curcumin significantly increased and Bax and malondialdehyde levels significantly decreased compared to control groups (p<0.05). This difference was not significant for sperm samples treated with 10 mg/ml curcumin.
Conclusion: Dose-dependent administration of curcumin decreased oxidative stress and lipid peroxidation and increased anti-apoptosis proteins in freeze-thawing sperms.
Niloofar Sadeghi , Marzieh Tavalaee , Mohammad Hosein Nasr- Esfahani ,
Volume 18, Issue 1 (3-2018)

Infertility, especially in the last decade, has been rising as a global problem, affecting approximately 10-15% of the world's population. The abundance and origin of various types of infertility are different. Studies have shown that reactive oxygen species (ROS) are involved in infertility, in particular in male infertility. Although ROS is essential for normal physiological functions of sperm such as capacitation, hyper activation, acrosome reactions, and binding to the oocyte, excessive levels can be one of the main causes of defective sperm function, which not only impairs the health of sperm DNA, but also affects fertilization via oxidation of proteins, in particular the fatty acids of the sperm cell membrane. Also, the elevated ROS levels facilitate sperm DNA damage, which leads to activation of apoptotic pathway and cell death. Therefore, the quality of semen is functionally reduced. Since the oxidative damage to sperm DNA is associated with both miscarriage and development abnormalities in the offspring, it is essential to find new strategies to recognize the cellular and molecular biology of sperm. Therefore, considering different mechanisms of oxidative stress affecting sperm can contribute significantly to the etiology of male infertility

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