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Showing 7 results for Metastasis

Khalil Rostami,
Volume 2, Issue 2 (6-2002)

  Hydatic cyst is caused by Echinococus worm, that involves any part of the body, sometimes it is presented in organs such as thyroid, breast and other organs. Axillary that may be consider adenopathy or metastasis and do biopsy, which so there is danger of leakage, anaphylaxis or relaps.

 The reported case was 39 years old female presented with painless axillary mass without inflammation since 19 years ago that slowly enlarged. Physical findings suggested hydatid cyst which was confirmed with ultrasonography and IHA for Echinococus granolosus and other diseases ruled out. She was operated and she had no relapses after 1.5 years follow up.

Shahrbanoo Keihanian , Zahra Fotokian , Maryam Zakerihamidi,
Volume 13, Issue 2 (6-2013)

  Background & Objectives: Bone metastases induce harmful potential complications on the life of patients. Pamidronate reduces skeletal complications in patients with bone metastases. This study evaluated the effect of Pamidronate on bone pain in cancer patients with bone metastasis.

  Methods: This was quasi experimental study carried out at Imam Sajjad hospital in Ramsar on 41 patients with malignancy by convenience sampling. In this study 90 mg of Pamidronate was injected intravenously each month for 3 months. Data collection was done through demographic and clinical data questionnaire and visual analog scale . Data of before and after intervention were compared. Statistical analysis was performed using paired t tests, chi-square, Friedman and Wilcox on tests with SPSS version 11.5 and p<0.05 was considered significant.

  Results: Statistical analysis showed that the most age group (36%) was 50-59 years and the most patients (65.9%) were female. The most common type of cancer was breast (43.9%) and the most common bone metastasis point in the most of patients (65.9%) was diffuse. The most common sites of pain associated with bone were sternum, ischium and T3-T4 vertebrae. Before treatment, the most patients (80.5%) complained of moderate pain whereas after treatment, the majority of them (41.5%) complained of low pain. The results of this study indicated that there was significant difference (p=0.032) between consumption of NSAIDs before and after treatment, but there was no significant difference between consumption of o pium before and after treatment (p=0.096).

  Conclusion: Pamidronate is effective in prevention of losing bone, reduced pain and analgesic consumption . So i t can be used as primary and routine treatment .

Shima Khajouee Ravari , Behzad Baradaran,
Volume 16, Issue 2 (7-2016)

Background & objectives: Prostate cancer is one of the main reasons of death between men. Although there are many methods for treatment of this cancer but most of the patients still are died of the postoperative recurrence and metastasis of disease. Over expression of HMGA2 gene was observed in many human malignancies such as colorectal cancer, thyroid, pancreatic carcinoma and lung cancers. The aim of this study was to investigate the effect of HMGA2 specific small interfering RNAs (siRNAs) on viability and apoptosis in PC3 prostate adenocarcinoma cell line. 

Methods: siRNA transfection was performed with liposome approach. The cytotoxic effects of siRNA were determined using MTT assay on the PC3 cells and apoptosis was quantified using TUNEL assay.

Results: Transfection with siRNA significantly suppressed the expression of HMGA2 gene in dose dependent manner after 48 hours resulting in spontaneous apoptosis. Moreover, siRNA transfection had effects on prostate cancer cells viability.

Conclusion: Our results suggest that the HMGA2 specific siRNA effectively decreases prostate cancer cells viability and induces apoptosis in this cell line. Therefore it can be considered as a potent adjuvant in prostate cancer therapy.

Shahla Mohammad Ganji , Mohammadnaser Molapour , Golamreza Javadi , Eisa Jahanzad,
Volume 16, Issue 3 (10-2016)

Background & objectives: KAI1 is a tumor suppressor gene and inhibitor of metastasis in a wide range of malignancies. While it is ubiquitously expressed in normal tissues, KAI1 expression subjects to the down regulation in tumors. The present research aims semi-quantitative evaluation of KAI1 mRNA expression in Iranian patients with colorectal cancer (CRC) and correlation between expression levels of KAI1 and stage oftumorigenesis, especially metastasis and invasion of CRC as well as pathologic factors of patients.

Methods: RT-PCR was done by specific primers for KAI1 and β-actin genes on the 80 tumor tissues and 14 normal tissues as fresh samples which obtained from 80 unrelated patients referred to Imam Khomeini Hospital.  

Results: According the results, 51.2% and 48.8% of the sample were on and off for KAI1 gene expression, respectively. As a detail, 97.3% of samples in the stage 3 and 4 and 94.5% of metastatic phases samples showed no expression of this gene. Statistical analysis showed that there is a significant difference of the KAI1 expression between four groups of samples; normal, stage 1, 2 and 3 (p<0.05). Also a significant difference was observed between semi-quantitative KAI1 expression and degree of spread to regional lymph nodes (p=0.02) as well as semi-quantitative KAI1 expression and metastasis (p=0.000001).

Conclusion: A significant difference between semi-quantitative expression of KAI1 and degree of spread to regional lymph nodes (p=0.02) and metastasis (p=0.000001) was observed.

Elshan Bazhan , Behzad Baradaran,
Volume 17, Issue 2 (7-2017)

Background & objectives: Prostate cancer is one the most common cancer in men whose incidence is increasing in many countries. According to the studies, decreased expression of miR-143 has been reported in prostate cancer. In this study, we replaced miRNA-143 in prostate cancer cells by vector based miRNA-143 and evaluated its inhibitory effects on migration of prostate cancer cells (PC3).
Methods: MTT assay was performed to reach an inhibitory concentration of Genticin antibiotic (G418 (in PC3 cells. Then, miRNA-143 vector was transfected into PC3 cells via JetPEI transfection reagent. The transfected cells were selected by G418 antibiotic according to a 2-week treatment with IC50 concentration. Then, the expression level of miRNA-143 was measured by qRT-PCR method. To evaluate the effect of miRNA-143 in inhibition of migration, scratch wound healing assay was performed.
Results: Results of MTT assay showed the IC50 level of G418 on PC3 cells was obtained to be 141.9 μg / ml. The results of qRT-PCR indicated increased expression of miRNA-143 in PC3 cells transfected with miRNA-143 compared to control cells. Finally, the results of wound healing assay showed migration reduction in transfected cells compared with control cells (empty vector).
Conclusion: The results showed that miRNA-143 play an important role in cell migration during prostate cancer metastasis, and it can be a good candidate for molecular treatments.
Shahriar Hashemzadeh , Davood Imani , Reza Javad Rashid , Mohammad Kazem Tarzamani , Sajjad Pourasghary ,
Volume 18, Issue 1 (3-2018)

Background & objectives: 10 year survival rates for thyroid cancer is about 90%, but papillary thyroid cancer often spread to regional lymph nodes resulting in survival rate falls below 90%. In patients with thyroid cancer, cervical lymph node metastasis risk is about 20 to 50 percent. The aim of this study was to evaluate the association between ultrasound results and the involvement of lymph nodes before thyroidectomy and compare it with the pathologic response after thyroidectomy in patients with non-medullary thyroid cancer.
Methods: 60 patients with thyroid cancer were randomly selected and entered into the study. Ultrasonographic examination of cervical lymph nodes was performed by two radiologists using an ultrasound machine in all patients diagnosed with thyroid cancer. Patients underwent total thyroidectomy and neck lymph node dissection by surgeon.
Results: In papillary thyroid cancer, there was a significant relationship between ultrasonographic results and pathologic outcomes in determining the presence of cervical lymph node metastasis before thyroidectomy. And also, there was a significant relationship between the results of ultrasonography and pathologic findings in determining the location of affected lymph nodes.
Conclusion: Compared to histological examination, ultrasonography can be a useful tool in determining the location of affected cervical lymph node in thyroid cancers before surgery.
Behzad Baradaran, Saeed Noorolyai ,
Volume 18, Issue 4 (3-2019)

Backgrounds & objectives: Colorectal cancer (CRC) is the third leading cause of cancer death worldwide. Micro RNAs are a group of non-coding small RNAs that inhibit the translation of target mRNA. MiR-146a-5p, as a tumor suppressor, has abnormal expression in many cancers. In this basic research, our goal was to restore the expression level of miR-146a-5p to normal level and to investigate its effect on the expression of the MMP9 gene in HT-29 cells.
Methods: this study evaluates the effect of transfection of miR-146a-5p in HT-29 cell line. At first, the HT-29 cell line from colorectal cancer was cultured in RPMI-1640 culture media and then  were transfected with miR-146a-5p using Jet-PEI reagent. qRT-PCR technique was employed to evaluate the expression level of miR-146a-5p and MMP9 genes. The statistical analysis was performed using GraphPad Prism 6 software.
Results: According to the obtained data, the onset of the invasion and metastasis, in particular, at the final stage of colorectal cancer may be related to a reduction in the expression of miR-146a-5p. The results of the qrRT-PCR test showed that by increasing the expression level of miR-146a-5p in HT-29 cells, the expression level of MMP9 gene decreased in the miR-146a-5p transfected group compared to the control group.
Conclusions: According to this study, activation of metastatic pathways was due to the down regulation of miR146a-5p. Accordingly, miR-146a-5p can inhibits migration of these cells through down-regulating the expression of metastasis-related genes. Hence, miR-146a-5p can be a new diagnostic biomarker and new therapeutic target for CRC.

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مجله دانشگاه علوم پزشکی اردبیل Journal of Ardabil University of Medical Sciences
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