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Showing 3 results for Sheikhkanloui Milan
Hamid Sheikhkanloui Milan,
Volume 12, Issue 3 (autumn 2012)
Background & Objective: It's been considered that midbrain region namely rostral ventromedial medulla (RVM) is the coring point of modulation of pain. The periaqueductal gray (PAG) matter has been documented to act as antinociceptive along with RVM. However, there are evidences demonstrating that RVM get a few connections from the cuneiformsnucleus (CnF) which might indicate CnF does have the same mechanism as PAG. Thus in this study, we tried to explore the antinociception effects of CnF and GABAA circuits.
Methods: Rats were anesthetized with Thiopental drug. Using the stereotaxic apparatus and Paxinos atlas, the point representing for CnF location over the scalp has been designated. One week after the surgery, rats were fully prepared for tests. Animals were put in three groups. First for control group, 0.5 µl of normal saline was injected into the CnF. Later, a few nanograms of bicucculline (as a GABAA antagonist) were administered into the CnF, dose dependently.
Results: From the results, it has been shown that CnF has a role in antinociception effect since the injection of bicucculline increased tail flick latency (TFL) and even further increased TFL when administered dose dependently (with high doses).
Conclusion: According to results of current study, CnF has a significant role in pain modulation and circuits of GABAergic system also plays a key role on its antinociception effect.
Sh Heydari Tajaddod, N Najafzadeh, M Mahdavi Rad, H Sheikhkanloui Milan, H Kalarestaghi, V Nejati,
Volume 15, Issue 2 (summer 2015)
Background & objectives: Hair follicle stem cells (HFSCs) are multipotent and various types of HFSCs were introduced. HFSCs separation using cell surface markers is one of the interesting strategies in the replacement of old methods. In this study, we used magnetic activating cell sorting (MACS) to separate HFSCs.
Methods: In this study, HFSCs were isolated from Balb/c mice and dissected under an invert microscopy, and bulge area isolated and the bulge cells cultivated about 14 days. The CD34 positive cells isolated using CD34 monoclonal antibody and magnetic activated cell sorting system (MACS), then the cells incubated in DMEM/F12 and 10% FBS. The CD34 positive cells counted using a neubauer slide and evaluated under a fluorescent microscopy.
Results: Here, we isolated CD34 positive cells using MACS and 12±1. 04% of HFSCs were CD34 positive and we found that, CD34 positive cells survived during 7 days cell culture in vitro.
Conclusion: The results show that MACS is useful to increasing density gradient of cells in vitro.
Yavar Mahmoudzade, Seyedeghbal Motavalibashi, Khaiam Bamdad, Eslam Zabihi, Hamid Sheikhkanloui Milan , Nasrin Hamidi,
Volume 17, Issue 1 (spring 2017)
Background & objectives: Terfezia boudieri (TB) has more than 15 types of proteins, gallic acid, catechins, flavonoids, tannins and other compounds such as beta-carotene and linoleic acid. Some of these compounds have antioxidant and anti-hyperlipidemia effects. Therefore, this study aimed to examine the antioxidant and protective effects of this fungus on the liver injury induced by carbon tetrachloride.
Methods: 30 male Wistar rats were divided randomly into 5 groups (each group containing 6). Group 1 (normal control group) and group 2 (control CCl4) were given normal saline for 14 days. Group 3, 4 and 5 received 500, 1000 and 1500 mg/ kg doses of methanol extract of TB for 14 days, respectively before the injection of carbon tetrachloride. In the fourteenth day, all groups except group 1 were given 1.5 ml/kg of carbon tetrachloride (V / V 1: 1 dissolved in olive oil). Group 1, instead of carbon tetrachloride, received 1.5 ml/kg of olive oil on the fourteenth day.
Results: Injection of carbon tetrachloride in rats increased serum activity of ALT, AST, ALP and serum degrees of triglycerides, VLDL, LDL, HDL, total cholesterol, glucose, and reduced HDL and total antioxidant capacity (TAC); while pre-treatment with methanol extract of TB significantly reversed the changes.
Conclusion: The results of this study showed that methanol extract of TB can protect the liver against oxidants and free radicals produced by carbon tetrachloride metabolism.