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Showing 3 results for Sabokbar

Jalal Solati, Azar Sabokbar, Jalil Vand Yousefi , Nasrin Kalkhorani ,
Volume 10, Issue 4 (winter 2010)
Abstract

Background & Objectives: Previous studies demonstrated that selected probiotic bacteria elicit beneficial effects in animals. Probiotic bacteria inhibit pathogens growth in the gut, improve lipid metabolism and activate immune system of animals. In the present study Enterococcus spp were isolated from Iranian traditional cheese and their effects on intestine pathogens (Shigella dysenteriae, Escherichia coli and Salmonella Typhimurium) growth, serum lipids level and activation of immune systems in mice were studied.

  Methods: Iranian cheese samples were collected from Ardabil province. Enterococci spp were isolated using selective culture mediums and identified using API kites. Inhibitory effects of isolated Enterococci on growth of Pseudomonas aeruginosa and intestine pathogens (Shigella dysenteriae, Escherichia coli and Salmonella typhimurium) were tested using agar well method . In order to study probiotic activities of isolated bacteria in live animals, NMRI mice were divided into different groups and Enterococci was administrated orally (1 ML/mouse) with doses equal to 2 (6×108 cfu/ml) 3 (9×108 cfu/ml) and 4 (12×108 cfu/ml) MacFarland standard for 2 weeks. After two weeks continues treatment, blood samples were collected from retroorbital sinus and serum levels of cholesterol, triglycerides, LDL and HDL measured using enzymatic method. Interleukins (IL-2, IL-6 and IL-10) levels were measured using ELISA kites.

  Results: Results of this study demonstrated that treatment with faecium species decreases serum cholesterol and increases serum IL-10 level, while it has not showed significant effects on serum levels of glucose, triglycerides, IL-2 and IL-6 (p<0.05). Administration of faecalis species have no significant effects on lipid levels of serum ( p <0.05). Moreover, results revealed that treatment with faecalis species increased IL-6 and IL-10 ( p <0.05). None of the species affected pathogens growth significantly ( p <0.05).

  Conclusion: The results obtained from current study demonstrate that continues treatment with both species can affect immune functions of animal by altering the cytokines profile and treatment with faecium species decreases serum level of cholesterol.


Fatemeh Hadadi, Azar Sabokbar, Mahrouz Dezfulian ,
Volume 14, Issue 2 (summer 2014)
Abstract

  Background & objectives: Trichophyton rubrum is one of the most common pathogeniccause of dermatophytosis. One of the drugs which have been prescribed widely for fungal infections is terbinafine which belongs to allylamines group of antifungal agents. Recently molecular typing methods have been developed for answering the epidemiological questions and disease recurrence problems. Current study has been conducted on 22 isolates of Trichophyton rubrum obtained from patients randomly. Our aim was the investigation of correlation between genetic pattern and sensitivity to Terbinafine in clinical isolates of Trichophyton rubrum.

  Methods: Firstly the genus and species of isolated fungi from patients have been confirmed by macroscopic and microscopic methods, then, the resistance and sensitivity of isolates against drug have been determined using culture medium containing defined amount of drug. In next step fungal DNA has been extracted by RAPD-PCR (random amplified polymorphic DNA) with random sequences of 3 primers.

  Results: Each primer produced different amplified pattern, and using each 3 primers differences have been observed in genetic pattern of resistant and sensitive samples using each 3 primers, but there was no bond with 100% specificity.

  Conclusion: The 12 sensitive isolates which didn’t grow in 0.1 mg concentration of drug, also had limited growth at the low concentration of drug. Ten resistant isolates which grew in 0.1mg/ml of drug, in lower concentration of drug were resisted. RAPD analysis for molecular typing of Trichophyton rubrum seems to be completely suitable.


F Hadadi, A Sabokbar, M Dezfulian , A Bakhtiari ,
Volume 15, Issue 2 (summer 2015)
Abstract

  Background & objectives: Trichophyton rubrum is one of the most common pathogenic causes of dermatophytosis. One of the drugs prescribed for fungal infections is fluconazole which belongs to Azoles group of antifungal agents. Recently molecular typing methods have been developed for answering the epidemiological questions and disease recurrence problems. Current study has been conducted on 22 isolates of Trichophyton rubrum obtained from patients randomly. Our aim was the investigation of correlation between genetic pattern and sensitivity to Fluconazole in clinical isolates of Trichophyton rubrum .

  Methods: Firstly the genus and species of isolated fungi from patients have been confirmed by macroscopic and microscopic methods. Then, the resistance and sensitivity of isolates against drug have been determined using culture medium containing defined amount of drug. In next step fungal DNA has been extracted by RAPD-PCR (random amplified polymorphic DNA) with random sequences of 3 primers.

  Results: Each primer produced different amplified pattern, and differences have been observed in genetic pattern of resistant and sensitive samples using each 3 primers, but there was no bond with 100% specificity.

  Conclusion: The 12 sensitive isolates which didn’t grow in 50µg/ml concentration of drug, also had limited growth at the lower concentration of drug. Ten resistant isolates which grew in 50µg/ml of drug, also showed resistant to lower concentration of drug. There are differences in genetic pattern of resistant and sensitive samples. RAPD analysis for molecular typing of Trichophyton rubrum seems to be completely suitable.



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مجله دانشگاه علوم پزشکی اردبیل Journal of Ardabil University of Medical Sciences
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