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Hmid Mirzaei, Soltanali Mahboob , Bahram Amooghli Tabrizi , Mehran Mesgari Abbasi , Mohammad Manafi ,
Volume 8, Issue 2 (Summer 2008)
Background & Objective: Hyperlipidemia is one of the main causes of cardiovascular diseases which have a high mortality throughout the world. Consumption of products containing specific strains of microbes as probiotics, produces positive effects in the host by balancing the flora of the digestive system. L.casei is a probiotic and the aim of this study was to evaluate the effects of consumption of milk containing L.casei on serum lipid profile of rats fed high lipid diet.
Methods : This was an experimental study in which 30 male albino wistar rats with a body weight of 200 ± 15 gr were randomly allocated to two groups of treatment and control each containing 15 rats and adapted to a high lipid diet (11.47%) and water containing milk (25%). Rats in both groups received high lipid diet and water containing 25% milk for 48 days with the difference that rats in the treatment group received water containing L.casei considering that water consumption is created throughout the experiment, the amount of L.casei added to milk was calculated to each rat consumed 109 CFU bacteria per day.
Results : Independent t-test revealed that at a level of α= 0/05 mean levels of total cholesterol and LDL-C of rats in the treatment group was significantly lower than that of control group (p<0/05), but the mean levels of triglyceride, HDL-C and VLDL-C was not significant. Also increase in body weight was significantly higher in the treatment group compared to the control group (p<0/01).
Conclusion : Long term daily consumption of milk containing L.casei improve serum lipid profile by lowering total cholesterol level and also increase the rate of body growth.
َarash Mehri Pirayvatlo , Reza Alipanah-Moghadam , Mohammad Mazani, Farideh Manafi, Vadood Malekzadeh, Ali Nemati, Abbas Naghizadeh Baghi ,
Volume 16, Issue 4 (winter 2017)
Background & objectives: Iron overload is involved in the pathophysiology of many diseases including diabetes. In fact, the excess iron by creating free radicals makes damage to pancreas and leads to insulin resistance and diabetes. Andrographolide extract has hypoglycemic and antioxidant properties. This study has surveyed the effects of andrographolide on blood glucose and lipid profile in rats with secondary iron overload.
Methods: In this experimental study, 36 male Wistar rats were randomly divided into 6 groups: the healthy control group, secondary iron overload group, secondary iron overload groups treated with a dose of 3.5 and 7 mg/kg of andrographolide extract, and andrographolide groups treated with a dose of 3.5 and 7 mg/kg of extract. Iron and extract were injected for 6 and 12 days, respectively. Blood samples were taken for measurement of blood glucose and lipid profiles. Data were analyzed using ANOVA test.
Results: The pathological results of samples from liver of animals receiving iron showed that the iron was deposited in the liver tissues. Iron injection significantly increased blood glucose levels compared to healthy control group (p<0.05). In the iron overload group, andrographolide extract with a dose of 3.5 mg/kg or 7 mg/kg significantly decreased blood glucose levels (p<0.05). Iron injections did not increase the serum triglyceride and cholesterollevels. Injections of andrographolide extract with a dose of 3.5 mg/kg and 7 mg/kg, significantly decreased the cholesterol levels compared to iron receiving group (p<0.05).
Conclusion: Results of this study showed that the andrographolide with different doses may be effective in the treatment of diabetes by reducing serum glucose and cholesterol levels.
Farid Manafi , Reza Alipanah-Moghadam , Ali Nemati, Vadood Malekzadeh, ,
Volume 17, Issue 4 (winter 2018)
Background & objectives: Andrographolide has potent antioxidant properties. There is still not a complete understanding of the antioxidant effects of andrographolide. In this study, the effect of andrographolide on the serum antioxidant activity of superoxide dismutase and catalase was investigated in the rats with secondary iron overload.
Methods: In this experimental study, 32 rats were divided into 4 groups of 8 each. The groups included: 1-Control 2- Receiving iron 3- Receiving iron treated with 7 mg/kg andrographolide 4- Receiving 7 mg/kg andrographolide. The serum activity of superoxide dismutase and catalase and serum level of malondialdehyde (MDA) was measured using colorimetric method. Prussian blue was used to evaluate iron deposition in liver.
Results: The results showed that iron was deposited in the liver. The activity of superoxide dismutase and catalase in the secondary iron receiving group, compared to control group, was significantly increased (p<0.05). The activity of catalase was significantly decreased in the group receiving iron and treated with andrographolide, compared to the control and iron receiving groups (p<0.05). Also, the activity of superoxide dismutase in the group receiving iron and treated with andrographolide decreased significantly compared to the iron receiving group (p<0.05). Serum levels of malondialdehyde in the iron receiving group were higher than the control group (p<0.05). Serum levels of malondialdehyde in the group receiving iron and treated with andrographolide, compared with the iron receiving group, showed a significant decrease (p<0.05).
Conclusion: Andrographolide (7mg/kg) reduced the serum activity of catalase and superoxide dismutase and malondialdehyde serum levels in rats with secondary iron overload.