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Showing 21 results for Malek

Javad Mikaeli , Mortaza Khatibian , Zohre Movahhedi, Hosein Gorbani Behrooz , Reza Malekzadeh,
Volume 5, Issue 1 (spring 2005)
Abstract

 Background & Objectives: Cholangiocarcinoma is a malignant tumor of the biliary tract epithelium. Although it is not a common tumor, it has a high mortality rate so that the five-year survival of the patients is only 5%. Most patients are found to have irresectable tumor on initial presentation. These have led to an emphasis on the role of palliative treatment.

 Methods: 43 patients with Cholangiocarcinoma (from 1996-2003) based on ERCP were included in this study. Demographic aspects, clinical and Para clinical manifestations as well as the risk factors were investigated and survival rates of the patients were compared with or without stenting of the biliary tract.

 Results: 27 patients (62.8%) were male and 16(37.2%) were female with mean age of 65. Ulcerative colitis accompanied with PSC was diagnosed in just one case. Jaundice, weight loss and pruritus were the main complaints of the patients. Twenty-seven cases received stent in biliary tract through ERCP while no treatment was performed in other patients due to technical failure or patients’ disagreement. Mean survival time for all patients was 125 days. There were no statistically significant difference between survival rate of the patients with stent and without it.

 Conclusion: In this study cholangiocarcinoma in our patients was less frequently seen to accompany UC and PSC. Curative surgical resection is not possible in the majority of cases, and palliative treatment only decreases the severity of the symptoms but has no significant effect on survival.


Kazem Sakha, Arman Malekian,
Volume 5, Issue 2 (Summer 2005)
Abstract

 Background & Objectives: Regarding the admitted cases at early infancy in Tabriz Pediatrics Medical Center, this research was carried out to determine the role of Hep.B. vaccination in the incidence of ITP.

 Methods: We studied all patients’ admission files in Tabriz Pediatrics Center from 1993 to 2002. The patients were under 6 months of age and hospitalized with the impression of ITP. There were 25 infants whose ITP was diagnosed upon their clinical findings, blood counts and bone marrow aspiration and confirmed by exclusion of other causes of thrombocytopenia. Hep. B. vaccination has been included in routine immunization in Iran since 1993. In order to compare the incidence rate of ITP before and after 1993, we also sought all ITP patients’ files at the same age group who had been admitted to Tabriz Pediatrics Center during the decade before the beginning of routine Hep.B. vaccination (i.e. 1982 to 1992).

 Results: This study shows a considerable increase in incidence of ITP in infants under 6 months of age between 1993 asnd 2002 compared to the decade before the beginning of Hep.B.vaccination, which is statistically significant (P=0.0029).

 Conclusion: Although there is no facility to measure antibodies in Iran and many other countries, and this is one of the reasons for lack of proof for existence of a relationship between vaccination and ITP incidence, the study of cases in two different periods may strengthen the role of Hep.B. vaccination in the occurrence of ITP at early infancy.


Eabbas Yazdanbod, Fateme Samadi, Reza Malekzade, Masuood Babaie , Manoogehr Iranparvar , Ahmad Azami ,
Volume 5, Issue 2 (Summer 2005)
Abstract

  Background & Objectives: Recent studies in Ardabil province indicated that upper GI cancer is the most common kind of cancer in this province. Due to the lack of any study concerning the survival rate of the patients in this province, the present research set out to evaluate the effect of demographic features of GI cancers, risk factors, histopathology type, the site of tumor and treatment on the survival rate of these patients.

  Methods: In this descriptive-analytical and prospective study, we followed up 420 upper GI cancer cases (141 esophagus cancers and 279 stomach cancers) who were initially diagnosed in the only subspeciality GI clinic of Ardabil province during four years from 2000 to 2003.

  Results: The information related to 299 patients was recorded. The dates of death of 55 cases were found referring to the provincial death registration program while the addreses of 67 cases were not found. The 1-4-year survival rates in the patients with upper GI cancer in Ardabil province were 25.9%, 11.1%, 4.5% and 1.8% respectively. In this study men had a lower survival rate than women and patients with esophagus cancer had a longer survival rate. In the patients with stomach cancer "Signet ring cell type" histopathology had the lowest survival rate. The patients who had undergone surgery survived longer than other patients with no resectional surgery treatment. Histopathology grading, age at first diagnosis, positive familial history of GI cancer, as well as cigarette, alcohol and opium consumption had no effect on survival rate of these patients.

  Conclusion: Overall survival rate of our patients is very low this condition might be related to late diagnosis and less treatment facilities in Ardabil province.


Abbas Yazdanbod , Manoochehr Iran Parvar , Firooz Amani , Reza Malekzadeh , Saeid Salavati ,
Volume 6, Issue 3 (Autumn 2006)
Abstract

 Background and Objectives: Gastric cancer is the second most common cause of cancer in terms of mortality in the world. Ardabil province, in northwestern Iran, has the highest rate of gastric (predominantly cardia) Adeno carcinoma in Iran. The most common site of upper gastrointestinal cancer in Ardabil is cardia region (its right-side wall). Therefore this study aimed to investigate comparatively histopathology of right and left side of cardia and z-line considering the incidence of inflammatory and precancerous lesions.

 Methods: This descriptive and cross-sectional study was performed on one hundred adults who referred to Aras clinic for endoscopy. Endoscopy was done with video endoscope and a single biopsy was taken from right and left side of cardia and z-line respectively and one from antrum for H.Pylori. All the samples of the biopsy material were fixed in formalin in separate bottles and pathologic study of the samples with regard to inflammatory and precancerous lesions were done according to Sydney classification of gastritis. The collected data wereanalyzed by SPSS software using descriptive and analytical statistics.

 Results: 61 (61%) patients were male and 39 (39%) female. The age range of patients was 19-80 years old (standard deviation 53±16.2). Mild to severe inflammation in left and right side of cardia and z-line was 82.6%, 84%, 97.1%, 93%, mild to severe atrophy in 22.8%, 28.7%, 26.5%, 24.4% and metaplasia type 1 to 3 in 2.2%, 6.4%, 2.9%, 12.2% respectively. Suspected dysplasia in cardia and z-line was only in one case. Analysis of these variables with McNamara, Wilcoxon and Lambda tests showed no statistically meaningful difference considering the histopathological findings in left and right side wall of cardia and z-line

 Conclusion: This study showed no statistically meaningful difference between right and left wall of cardia and z-line considering the incidence of inflammatory and precancerous lesions. This observation is not consistent with high incidence of right sided cardia cancer. Further study with multiple biopsy sample and more sensitive methods is recommended for the diagnosis of precancerous lesions.


Jamshid Yousefi, Abdolreza Malek, Seyedmorteza Mirzadeh , Samaneh Davoodi ,
Volume 6, Issue 3 (Autumn 2006)
Abstract

 Background and Objectives: Jaundice is a common disorder in preterm and term newborns and G6PD deficiency is one of its etiologic agents. With respect to high incidence of G6PD deficiency in Iran, this study was conducted to determine the clinical correlation of this deficiency with time of jaundice onset and mean level of serum bilirubin. Management and complication rates are also evaluated in these neonates in comparison with those without this deficiency.

 Methods: This is a case-control study performed through G6PD assay in icteric newborns admitted to neonatal intensive care unit in 22 Bahman hospital in Mashhad within 3 years. This case group was compared with the control group who were randomly selected from among icteric neonates without G6PD deficiency. The data were collected by a questionnaire and analyzed by SPSS.

  Results: This study was done on 505 icteric neonates. The case group consisted of 34 newborns (6.7%) with G6PD deficiency. M/F ratio in case group was higher than control group (P= 0.01). Mean level of total serum bilirubin was significantly higher in case group (P=0.047). There were no significant differences in duration of phototherapy and hospitalization between two groups, but exchange transfusion rate in case group was twice as much. Complications such as seizure and kernicterus in both groups were the same.

 Conclusion: Due to high incidence of G6PD deficiency in our study (6.7%) and significant incidence in female patients, determination of enzyme level is recommended in all newborns with jaundice regardless of their gender. Because of higher level of serum bilirubin in G6PD deficient patients, phototherapy must be done as soon as possible to avoid invasive procedures such as exchange transfusion.


Hesam Abdolhoseinpour , Mojtaba Malek , Mohammad Mohammadi , Gholamreza Bakhshandepour,
Volume 6, Issue 4 (Winter 2006)
Abstract

 Background & Objectives: Delayd post-traumatic intracranial hemorrhage (DTICH) is a kind of brain hemorrhage which occurs after the first six hours of the head trauma, and is not detected in the initial CT Scan taken from the patient in the first 6 hours after the trauma. As DTICH is one of the most important factors in occurance of secondary brain injuries in patients with head trauma, its early diagnosis and treatment could significantly decrease mortality and morbidity of these patientes. This diagnosis is a contraindication to anti-coagulant therapy. This study was conducted to determine the frequency of factors associated with DTICH incidence.

 Methods: This was a case-control study conducted in Semnan trauma center. In this research all the patients with head trauma who referred to Emdad hospital between 2002 and 2004 were studied and the data about the delayed post-traumatic intracranial hemorraege (20 cases) were included. Then, beside determining the incidence of DTICH in the patients with hemorrhage, some risk factors such as headache, contusion, reduction level of conciousness, skull fracture and impact intensity to the head were compared with a control group (40 cases) who had head trauma without primary or delayed hemorrhage. The groups were age and sex-matched. The data were analyzed using Chi-aquare and computing odds ratio.

 Results: This study showed that the frequency of DTICH was 10.6%. There was a meaningful relationship between DTICH and skull fracture, reduction of conciousness level, contusion and impact intensity. But, there was no meaningful relationship between DTICH and intractable headache.

 Conclusions: The results indicated that cases such as skull fracture, contusion, impact intensity and findings such as the reduction of consciousness in patients with head trauma (with no hemorrhage in the first CT scan), can be the risk factors for DTICH.


Farzaneh Delgoshaie , Farzaneh Maleki , Mohammad Ramezani , Jamshid Yousefy ,
Volume 7, Issue 2 (Summer 2007)
Abstract

 Background & Objectives: Resistance Strains of propionibacterium acnes is one of the most growing problems in acne treatment. Azelaic acid, due to the lack of bacterial resistance, could be a useful alternative in topical treatment of acne. The purpose of this study was to investigate the therapeutic effects and side effects of azelaic acid and its comparison with topical clindamycin in the treatment of acne.

 Methods: This randomized clinical trial study was performed on 100 patients with mild acne. The patients were divided in to two groups. One group was treated with azelaic acid 20% cream and the other one was treated with Dalacin 1% gel twice daily for 4 weeks.

 Results: Both drugs Produced significant reduction in inflammatory Lesions (for azelaic acid p=0/000 and for clindamycin p=0/000) and noninflammatory lesions (for azelaic acid P=0/001 for clindamycin p=0/033). Comparing the mean of decrease in inflammatory and noninflammatory lesions, there was not a significant difference between two groups (of Papule and Pustule P=0/864 of comedons P=0/239). So the efficacy of these two drugs was same. In comparing the sideffects, erythem was more common in azelaic acid users significantly (p=0/031) and skin dryness was more Common in clindamycin users significantly (p=0/000).

 Conclusion: Azelaic acid 20% cream as effective as clindamycin gel 1% in the treatment of mild acne.


Farzaneh Maleki , Farzaneh Delgoshaee , Mohammadreza Khakzad , Mohammad Wejdanparast, Behiyeh Zakerian ,
Volume 7, Issue 3 (Autumn 2007)
Abstract

  Background & Objectives: :Contact Dermatitis (C.D) is a common condition and 5.4% of population are suffering from it. Main treatments are based on emollients and corticosteroids. and in C.D cellular infiltration are mononuclears chiefly T. helper lymphocytes. The aim of this study was to have a new approach to herbals with new science covering and study the efficacy of alcoholic extracts of 4 herbals: henna, camomile cedar and aloevera in experimental C.D on mice.

  Methods: In a pilot experimental study first we chose 70 mice (ENMARY race, 50 ± 10gr weight as case group, then we shaved behind of their left ears (at the days 0 and 1) and rubbed DNCB 400 mg/ml on the skin.At the 10th and 14th day the same amount of DNCB again was rubbed on the left ear. On the right ear the same amount of olive oil in Acetone dissolver was rubbed,as control group. Then at the times of 2, 10, 24, 48, 72, 96, 120, 150 hrs after the 2nd contact, the mice were killed with Ether, cutting the ears from base and prepared pathological sections for study of cellular infiltration. In second stage alcoholic extracts of 4 plants were prepared, then herbal cream with 4% concentration in cold cream as pallish cream were made, with considering the results of first stage that showed the maximal infiltration was at 120 hr after contact, the creams were rubbed every 12hr for 7 days on 40 mice in 4 groups that had been sensitized before with DNCB. 2 groups (20 mice) were considered as control (10 mice received no drugs and 10 mice received cold cream). 60 mice were chosen in the second phase and in general 130 mice were investigated.

  Results: lymphocytic infiltration reduced after 7 days application of drugs for camomile group about 66/7%, cedar 50%, alaevera 12/5% and henna 0%

  Conclusion: Camomile chammazolen, Inositol had the best response (66/7% severe response) on improvement of contact dermatitis (P=0.02)


Mehrdad Biriya, Shahnam Arshi, Homayoun Sadeghi, Niloofar Malekpoor,
Volume 8, Issue 1 (Spring 2008)
Abstract

Background & Objective: one of the most important challenges during the recent century will be to decrease the global burden of accidents. In Iran although respiratory infections and diarrhea were major reasons of mortality rates, Accidents have come to be one of the main causes of child mortality. In rural areas of ardebil province burns constituted 75% of home
injuries in preschool aged children.
Methods: The study was conducted as a case-control study which included rural children of Ardabil under 6. Number of cases were 145 & controls were 289. Data analysis was done using SPSS 11 ver.
Results: 6.8% of case group mothers and 12% of control group mothers had academic education. Difference of mean for sill of kitchen and the ratio of sill of kitchen on sill of house and also difference of mean for sill of house per person were not statistically significant between case and control groups. No statistically significant difference was found in fuel type used cooking or heating between case and control group. No statistically significant
difference was found in heating device between Them. only in one third of houses in both groups separate place was available for cooking purpose and cooking was done in rooms.
Child presence in kitchen while cooking was 76.7% in case and 70.9% in control group. Child access to flamers like lighters and matches was 80% in case compared to 69% in control group. The difference was shown to be statistically significant. No statistically significant difference was found in using Valor's and picnic gas for cooking between case and control group.
Conclusion:chance of burn injury in preschool aged children in those houses that keepmatches and the lighters out of children reach was 1.8 times lower.
Hadi Peeridoghaheh, Marziyeh Aligholi, Mohammadhosein Dehghan, Parviz Maleknejad,
Volume 8, Issue 1 (Spring 2008)
Abstract

Background & Objective: Brucellosis is one of the most common zoonotic diseases in Iran and is endemic in all parts of the country. Patients recorded in 1988 were 71,051(132. 4 per 100,000). Brucella species are facultative intracellular bacteria, and therefore a limited number of antibiotics are effective against these organisms. The aim of this study was the
evaluation of in vitro sensitivity of various antimicrobial agents against 47 brucella melitensis strains isolated from blood culture.
Methods: The susceptibility of 47 Brucella melitensis isolates derived from clinical samples were tested in vitro. The minimum inhibitory concentrations (MICs) of the tested antimicrobials were measured by the agar dilution method.MIC90 and MIC50 values were defined as the lowest concentration of the antibiotic at which 90 and 50 percent of the isolates
were inhibited, respectively. The NCCLS criteria for slow growing bacteria were considered to interpret the results.
Results: Tetracycline (MIC50: 0.13μg/ml, MIC90: 0.25 μg/ml) and streptomycin (MIC50:0.003 μg/ml, MIC90:0.25 μg/ml) had the lowest MICs in vitro against the B. melitensis strains. Norfloxacin had the highest (8 μg/ml) MIC90 value. More than half
isolates presented reduced susceptibility to rifampin (MIC value: 2μg/ml).
Conclusion: Brucella isolates remain susceptible in vitro to most antibiotics used for treatment of brucellosis. There is no significantly important resistance problem for antibiotics targeted against Brucella species in Iran. However, since rifampin is commonly used for prevalent diseases such as tuberculosis, the regional susceptibility pattern of rifampin should be assessed periodically.
Masoud Maleki , Kazem Parivar , Mohammad Nabiyouni , Parichehr Yaghmaei , Mohammad Naji ,
Volume 9, Issue 2 (summer 2009)
Abstract

 Background and Objectives:Stem cells are characterized by the ability to renew themselves through mitotic cell division and differentiating into a diverse range of specific cell types. Wharton's jelly is the appropriate source of mesenchymal stem cells (MSCs) that have high differentiation competence. The aim of this study was differentiation of MSCs to lens fiber cells. In differentiation pathway of lens fiber cells, crystallins are expressed . Thus, crystallins can be used as differentiation marker of lens fiber cells.

  Method: In the current study MSCs were isolated from the mouse umbilical cord. It was minced into 1-2 mm3 fragments and then were incubated with collagenase type IA following pipetting for mechanical isolation of cells. Cell suspension was plated in 25 cm2 culture flasks. Alkaline Phosphatase detection kit was used for staining of undifferentiated UC-MSCs from passage I. In the experimental group MSCs were plated in the maintenance medium supplemented with bovine vitreous body (1:3 v/v) for induction. Protein lysate were prepared from cells on days 10 of induction and were analyzed on polyacrylamide gels and transferred to nitrocellulose membranes. Rat lens extract was used as a positive control. Anti-alpha A, alpha B crystallin, secondary antibody, vectastain ABC- kit (standard) and vector blue alkaline phosphatase substrate kit III were used.

  Results: Mouse umbilical cord MSCs had alkaline phosphatase activity. Morphological studies and separation of proteins in electrophoresis indicated that experimental group cells might probably differentiated into lens fiber cells.

  Conclusion: Mouse umbilical cord could be used as an appropriate source for MSCs. MSCs had fibroblast- like morph and experimental group indicated the presence of fiber-like cells that were long, thin, and parallel aligned. Electrophoresis and Western blot analysis showed there was a detectable expression of early developmental marker of lens fiber cells differentiation in experimental group.


Bahram Fatollahzadeh , Parviz Maleknejad, Abbas Bahador, Hadi Peeridogaheh,
Volume 10, Issue 1 (spring 2010)
Abstract

  Background & Objectives : This study was prepared to determine the frequency of Staphylococus aureus nasal colonization among intravenous drug abusers with respect to this fact that the rate of colonization is dependent on various factor including addiction behaviours. There wasn't any native study on this subject. The purpose of this study was to prepare the basic data of S.aureus nasal colonization among intervenous drug abusers, in order to reducing the incidence and nasal carriage rates of S.aureus infections.

  Methods : This was a prospective cross sectional study that included all of intravenous drug abusers who referred to three hospitals of Tehran from 2005 to 2006. Specimens for culture were obtained by swabbing anterior nares. Swabs were inoculated into nasal chapman broth and incubated at 35 °C for 48 hours. Isolated colonies were further subjected to identification and antimicrobial susceptibility testing. The related data were collected using patients` medical files and analyzed with using SPSS 11.

  Results : Staphylococcus aureus was grown i n 38 cultures (26.2%). We did not find any significant relationship between variables such as, economic condition, methods of using drugs, frequency of drug use, infection with different viruses, gender and colonization by Staphylococcus aureus.

  Conclusion: We observed lower nasal colonization with Staphylococcus aureus among patients. We suggest preparing similar study in order to clarify the role of different factors that have effect on the rate of nasal colonization with Staphylococcus aureus.


Asadollah Asadi , Arash AbdolMaleki, Farhood Najafi,
Volume 13, Issue 1 (spring 2013)
Abstract

  Background & Objectives: Polymers as drug carriers are recent advances in drug delivery and led to the new advent field that called polymer treatment. In the present study, the toxic and teratogenic effects of BDP18 were evaluated against chicken embryos as a model.

  Methods: The BDP18 tri-block copolymer (PLA-PEG2000-PLA) was synthesized. The compound solution was injected in triplicate examination, in the air sac of the eggs, at third day of incubation, and survived fraction of the embryos and Morphological and skeletal changes were recorded .

  Results: The survived fraction of the embryos depends on the compound concentration. In concentration of 20 mg/ml , 33.3% of the embryos were survived and the LD50 was 10.87 mg/egg . Morphological study of the treated embryos showed no abnormalities in embryos , and skeletal staining showed the deletion of caudal vertebrate in high concentration.

  Conclusion: The BDP18 copolymer had low toxic and teratogenic effects against the embryos, but it caused the deletion of caudal vertebrate at concentrations above the threshold (10 mg/ml). This polymer can be used as an effective drug -release system in low concentrations .


Saber Zahri, Masoud Maleki, Kamaladdin Hamidi , Seiyeh Mahsa Khatami ,
Volume 13, Issue 1 (spring 2013)
Abstract

  Background & Objectives: Stem cells are fundamental supporter of multicellular tissue. They allow blood, bone, gametes, epithelia, nervous system, muscle, and other tissues to be replaced by fresh cells throughout life. In recent years human Wharton’s jelly stem cells (WJSCs) have gained attention. They express a number of surface markers characteristic of mesenchymal stem cells. In this study, human Wharton’s jelly stem cells were isolated using explant method. To show the stemness property of these cells, three CD markers including CD105, CD44 and CD34 were tested.

  Methods: The umbilical cord samples were collected by Caesarian section at Arta Hospital in Ardabil. Cords were transferred in sterile conditions and stem cells were isolated using explant method. After log phase, cells were passaged then growth characteristics and CD105, CD44 and CD34 markers investigated by RT-PCR.

  Results: Separation of human Wharton’s jelly stem cells were started after 7 days. WJSCs in culture revealed two distinct cell population named Type 1 and Type 2. RT-PCR results showed that WJSCs were CD105+, CD44+ and CD34-.

  Conclusion: Human umbilical cord stem cells could be an alternative source instead bone marrow stem cells for cell therapy and tissue engineering. These cells have a fibroblastic appearance. Following the lag phase and into log phase respectively, cells grow easily in culture and retain stemness properties in higher passages.


Abbas Yazdanbod, Afshin Hooshyar, Rasool Nemati, Nasrollah Maleki, Gholamreza Hamidkholgh,
Volume 13, Issue 4 (Winter 2013)
Abstract

  Eosinophilic gastroenteritis is a rare disease characterized by focal or diffuse eosinophilic infiltration of the gastrointestinal system. The clinical presentation is depending on the involved area and its extension into the layers of the gastrointestinal tract. We repor ted a case of eosinophilic gastroenteritis in the setting of cryptogenic cirrhosis which initially appeared as a tumoral obstruction of the 3rd portion of duodenum which is relieved by surgical bypass and two years later presented as eosinophilic ascitis. The patient was treated with corticosteroid with full absorption of ascitis.


Mohammadreza Behvarz , Masoud Maleki , Mohammadreza Mashayekhi ,
Volume 14, Issue 4 (winter 2014)
Abstract

  Background & objectives: Adult stem cells are undifferentiated cells that replace dead or injured cells. There are adult stem cells in some regions of human tissues and hair follicle is one of the tissues that have adult stem cell source and these cells have an important role in hair life cycle. In this study, we investigated the isolation of hair follicle stem cells (HFSCs) and expression of mesenchymal stem cell markers on the isolated cells.

  Methods : Human hair follicles obtained from men scalp tissue by micro punch technique. Hair follicles isolated and cultured in culture flasks in DMEM-F12 + FBS. After outgrowth of stem cells from hair bulges, they analyzed by flow cytometry for detection of stem cell markers.

  Results: 23 to 27 days after isolation and culture of HFSCs in uncoated cell culture flasks, cell surface markers expression studied by flow cytometry. Flow cytometric analysis showed 25.26% Stro-1, 50.85% CD90, 45.24% CD105, 61.20% CD44, 8.20% CD45, 11.86% CD146, 2.72% CD106, 7.21% CD166 and 26.74% CD19 expression in HFSCs.

  Conclusion: In this study, isolated stem cells significantly expressed some of the mesenchymal stem cell markers higher than other markers. These markers give certain characteristics to HFSCs, and introduce the cells as an alternative option for cell therapy, tissue engineering and regenerative medicine.


َarash Mehri Pirayvatlo , Reza Alipanah-Moghadam , Mohammad Mazani, Farideh Manafi, Vadood Malekzadeh, Ali Nemati, Abbas Naghizadeh Baghi ,
Volume 16, Issue 4 (winter 2017)
Abstract

Background & objectives: Iron overload is involved in the pathophysiology of many diseases including diabetes. In fact, the excess iron by creating free radicals makes damage to pancreas and leads to insulin resistance and diabetes. Andrographolide extract has hypoglycemic and antioxidant properties. This study has surveyed the effects of andrographolide on blood glucose and lipid profile in rats with secondary iron overload.

Methods: In this experimental study, 36 male Wistar rats were randomly divided into 6 groups: the healthy control group, secondary iron overload group, secondary iron overload groups treated with a dose of 3.5 and 7 mg/kg of andrographolide extract, and andrographolide groups treated with a dose of 3.5 and 7 mg/kg of extract. Iron and extract were injected for 6 and 12 days, respectively. Blood samples were taken for measurement of blood glucose and lipid profiles. Data were analyzed using ANOVA test.

Results: The pathological results of samples from liver of animals receiving iron showed that the iron was deposited in the liver tissues. Iron injection significantly increased blood glucose levels compared to healthy control group (p<0.05). In the iron overload group, andrographolide extract with a dose of 3.5 mg/kg or 7 mg/kg significantly decreased blood glucose levels (p<0.05). Iron injections did not increase the serum triglyceride and cholesterollevels. Injections of andrographolide extract with a dose of 3.5 mg/kg and 7 mg/kg, significantly decreased the cholesterol levels compared to iron receiving group (p<0.05).

Conclusion: Results of this study showed that the andrographolide with different doses may be effective in the treatment of diabetes by reducing serum glucose and cholesterol levels.


Reza Alipanah_moghadam , Sara Hosseinzadeh, Ali Nemati, Abbas Naghizadeh Baghi , Vadood Malekzadeh,
Volume 17, Issue 2 (summer 2017)
Abstract

Background & objectives: The relationship between dietary patterns and esophageal cancer has not been documented yet. However, recent studies have shown that the Western dietary pattern is associated with an increased risk of esophageal cancer. The current study was performed a comparative evaluation of the food habits and serum nitrate level in patients with esophageal cancer and healthy individuals in Gorgan city.
Methods: In a case-control study, 44 patients with esophageal cancer (the case group) and 44 healthy subjects (control group) were selected for one year in Gorgan city. Anthropometric factors were measured, and then demographic and dietary data were recorded using general and food frequency questionnaires (FFQs), respectively. Fasting blood samples from both groups were collected to measure serum nitrate levels. Data were analyzed by independent t-test, Chi-square, Fisher, and Monte Carlo tests.
Results: the mean weight, body mass index (BMI) and serum nitrate levels were statistically lower in the case group than in control group (p<0.05). Salt intake, smoking cigarette or hookah was higher in patients than in control group (p<0.05). Based on nutritional habits , consumption of bread , rice, saturated fats, hot tea, sausages and industrial soft drinks was statistically higher in the patient group than control group, but intake of vegetables, fruits, unsaturated fats, honey and grilled meat was lower in patients  than control group (p<0.05).
Conclusion: Consumption of fast foods, hot tea and saturated fats may be associated with esophageal cancer. Serum nitrate levels were lower in patients than control group, probably indicating the need for further research in this field.
Farid Manafi , Reza Alipanah-Moghadam , Ali Nemati, Vadood Malekzadeh, ,
Volume 17, Issue 4 (winter 2018)
Abstract

Background & objectives: Andrographolide has potent antioxidant properties. There is still not a complete understanding of the antioxidant effects of andrographolide. In this study, the effect of andrographolide on the serum antioxidant activity of superoxide dismutase and catalase was investigated in the rats with secondary iron overload. 
Methods: In this experimental study, 32 rats were divided into 4 groups of 8 each. The groups included: 1-Control 2- Receiving iron 3- Receiving iron treated with 7 mg/kg andrographolide 4- Receiving 7 mg/kg andrographolide. The serum activity of superoxide dismutase and catalase and serum level of malondialdehyde (MDA) was measured using colorimetric method. Prussian blue was used to evaluate iron deposition in liver.
Results: The results showed that iron was deposited in the liver. The activity of superoxide dismutase and catalase in the secondary iron receiving group, compared to control group, was significantly increased (p<0.05). The activity of catalase was significantly decreased in the group receiving iron and treated with andrographolide, compared to the control and iron receiving groups (p<0.05). Also, the activity of superoxide dismutase in the group receiving iron and treated with andrographolide decreased significantly compared to the iron receiving group (p<0.05). Serum levels of malondialdehyde in the iron receiving group were higher than the control group (p<0.05). Serum levels of malondialdehyde in the group receiving iron and treated with andrographolide, compared with the iron receiving group, showed a significant decrease (p<0.05).
Conclusion: Andrographolide (7mg/kg) reduced the serum activity of catalase and superoxide dismutase and malondialdehyde serum levels in rats with secondary iron overload.

 
 
Pirooz Pour Mohammad , Reza Alipanah-Moghadam , Firooz Amani, Ali Nemati , Vadood Malekzadeh ,
Volume 18, Issue 1 (spring 2018)
Abstract

Background & objectives: Due to the widespread use of zinc oxide nanoparticles (ZnO-NPs) and their possible unwanted effects in biological environments, we made an attempt to investigate the effect of ZnO-NPs on the blood lipid profile.
Methods: In this study, 24 male Wistar rats were used. The rats were randomly divided into three groups: control group, the group receiving ZnO-NPs at 25 mg/kg (ZnO-NPs 25 mg/kg) and the group receiving ZnO-NPs at 50 mg/kg (ZnO-NPs 50 mg/kg). The duration of the test was two weeks. The blood samples were taken and the serums were separated. Serum levels of triglyceride, cholesterol, VLDL, LDL and HDL were determined using standard kits and methods.
Results: Based on the findings of this study, serum levels of triglyceride and VLDL in both ZnO-NPs receiving groups were significantly increased in comparison with the control group (p<0.05). Also,  the serum levels of HDL , LDL and LDL/HDL ratio decreased significantly in both ZnO-NPs receiving groups compared to the control group (p<0.05), while the  serum cholesterol levels did not change compared with the control group.
Conclusion: Our results showed that 25 and 50 mg/kg doses of ZnO-NPs had both harmful (increase triglyceride and VLDL and reduction of HDL) and beneficial (reduction of LDL and LDL/HDL ratio) effects on the blood lipid profile.

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مجله دانشگاه علوم پزشکی اردبیل Journal of Ardabil University of Medical Sciences
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