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Showing 6 results for Kazemi
Behnam Mohammadi Ghalehbin, Esmaeil Falah , Mohammad Asghar Zadeg, Abdol Hasan Kazemi, Ahmad Daryani, Firooz Amani, Saeide Amani, Mina Agazade, Rasool Abdollahi, Rouhollah Arab,
Volume 6, Issue 2 (Summer 2006)
Background & Objectives: Cryptosporidium is an intracellular – extracytoplasmic parasite that has taken much attention in last 20 years as a clinically important human pathogen. Cryptosporidial infection can be transmitted from fecally contaminated food or water and from animal-human or human-human contact. In immunocompromised persons, the illness is much more severe such as debilitation, fatigue, cholera-like diarrhea, severe abdominal cramps, low-grade fever, severe weight loss and Anorexia. Because there was no regional study about cryptosporidiosis in Ardabil, we carried out this survey to determine the prevalence of cryptosporidiosis among the children hospitalized in Ardabil.
Methods: This descriptive and analytical study was carried out on 371 patients in Sabalab and Aliasghar hospitals of Ardabil between 2004 and 2005. A questionnaire was filled for each patient. Stool samples were examined by concentrated formal - ether method and stained with modified Ziehl-Neelson method. The data were analyzed with SPSS (ver 11) using Chi-square test.
Results: We analyzed 371 stool samples from children with diarrhea. Cryptosporidium oocysts were detected microscopically in 15 samples. Its prevalence was 4.04% in infected patients. 66.7% of the infected ones were at the age of 6 to 24 months, 20% 25-48 months, and 13.3% 49-72 months.
Condusion: Because cryptosporidiosis was more prevalent at the age of 6-24 months, health education is more necessary for their mothers.
Behnam Mohammadi Ghaleh Bin , Esmaeil Fallah, Mohammad Asgharzadeh, Abdolhasan Kazemi ,
Volume 7, Issue 2 (Summer 2007)
Background & Objectives: Cryptosporidium is a coccidian protozoan parasite. This organism is one of the main causes of severe, long-time and life-threatening diarrhea in immunocompromised persons. It is also among the most prevalent diarrheal agents in children. Cryptosporidial epidemics occur after consumption of water which is contaminated by oosit species of cryptosporidium. Water is usually contaminated by animal feces or by drainage of waste water into drinking water resources.
Methods: In this study, from ten regions 200 water samples were collected, filtered by 1.2 micron papers and then positive samples were identified in terms of cryptosporidium using PCR method. Finally the related species were detected by RFLP method.
Results: Nested-PCR showed 8 samples were positive for cryptosporidium that according to RFLP of PCR products 5 samples belonged to cryptosporidium andersony, 2 samples belonged to cryptosporidium parvum bovine genotype and 1 sample belonged to cryptosporidium pig genotype.
Conclusion: Since Cryptoridium andersony and cryptosporidium parvum bovine genotype are the common species in animals and cryptospovidium swiss is seen in wild animals (pigs and boars), it so we conclude that animal reservoirs have the main role in the contamination of related water resources in this region.
Saeid Nafisi , Seyed Shamsaddin Athari , Reza Kazemi , Ehsan Hosseini ,
Volume 9, Issue 4 (winter 2009)
Background and objectives: During the last years the effects of pulsative electromagnetic fields have been studied in the human and animals nervous system. The using of these fields in today living has caused worriment in safety of human. Stress causes very problems in behavior and function of biological systems. In this survey the effects of electromagnetic fields on stress behaviors of rats have been studied.
Methods: In this study we aimed to investigate the effects of electromagnetic fields with 700 milli Gauss currency produced by triangle electrical currents with low (10 Hz) and high (110 kHz) frequencies on stress behaviors in 60 adult, male Wistar rats. The rats were divided to two groups of control and test. The animals were exposed to the field for 80 minutes and in order to evaluate the possible effect of electromagnetic fields the Tail pinch (T.P.) test was conducted. The results were analyzed using statistical tests.
Results: Analyzing of the results of response time and stress behavior by T-test. In the group exposed electromagnetic fields of triangular low waves (10 Hz) for 80 min the results were meaningful (p < 0.05). The response and stressed behavior with this field is decreased very much. The results of two groups after 80 min under this field that have a difference in frequency showed that effects of frequency in behavior was meaningless.
Conclusion: The results of this study showed that electromagnetic fields produce anti-stress effect in rats. The radio waves (A.W) have higher effects.
Abdolhasan Kazemi , Najibeh Akbari , Eiraj Asvadi, Jamal Aivazi , Jalil Vaez , Alireza Nikanfar , Hadi Maljaei , Hosein Koshafar, Iran Nokhah, Leila Nozamani,
Volume 10, Issue 2 (summer 2010)
Background & Objectives: Malassizia furfur (pityrosporum ovale/orbicular) and other related species are ethologic agents of tinea versicolor and pityrosporosis in normal individuals but fungal infections due these yeasts are a major cause of mortality in immunocompromised and cancer patients. Catheter-related fungemia or foliculitis is most common mycoses in immunocompromised cases, but malassezia Spp., has been frequently implicated as the causative agent of peritonitis, septic arthritis, mastitis, and sinusitis and variety ocular infections. In this study we surveyed Pityrosporom ovale in dandruff of patients with leukemia underlying chemotherapy.
Methods: Over a one year period, 100 scale samples were obtained from 50 patients with leukemia underlying chemotherapy. All samples were stained using Metilin Blue method. In direct microscopic examination, seeing budding yeast cells with certain numbers, (bottle bacillus) on epithelial cells were reported positive sample.
Results: Pityrosporosis were dtected in %78 patient with Leukemia. Most of patients were range of 21-30 years old (27%), that suffering from increased scale.
Conclusion: Malassezia fur fur is one of more common noncandidal yeasts causing a variety of fungal infection. This organism is a lipophilic yeast that colonizes superficially in human skin and causes superficial mycoses such as tinea versicolor, rarely catheter– related sepsis, foliculitis and other systemic mycoses. Most reported cases of systemic mycoses due to this yeast have been in neonates or adults with malignancy or immunocompromised patients, who were receiving parenteral lipids via a central vascular catheter, undergo chemotherapy and BMT. As pityrosporosis were positive in over than 82% of studied patients, suggested that for prevention of serious fungal infections and mortality in immunocompromissed patients, it must be considered a suitable anti fungal protocol for these cases such as using shampoo or other drugs containing antifungal agents for treatment of patient underlying chemotherapy.
Masoumeh Kazemi, Hedayat Sahraie , Mahnaz Azarnia , Hosein Bahadoran,
Volume 10, Issue 2 (summer 2010)
Background & Objectives: Previous studies indicated that morphine consumption during pregnancy could inhibit embryos development. Present study further evaluated the effects of oral morphine consumption on the maternal and fetal portion placenta cells development in Wistar rats.
Methods: Female Wistar rats (W: 170-200 g) were used in the present study. Morphine group were received morphine (0.05 mg/ml of tap water) after one night coupling with male rats for mating. On 14th, 17th days of pregnancy, the pregnant animals were killed with chloroform and the placentas and uterus were removed surgically and fixed in 10 % formalin. The fixed placentas and uterus were stained by H & E method and evaluated for their development. The thickness of layers, as well as number of the cells in both maternal and fetal parts of the placentas was determined by light microscopy and processed using MOTIC software.
Results: The results indicated that oral consumption of morphine compared to control group, increased the thickness of the layers in maternal portion and also, increased the number of the cells in both maternal and fetal portion of the placenta.
Conclusion: All together, oral morphine consumption may inhibit placenta cells development and disturb their natural functions. These abnormalities observed in the placenta by opioid addicted pregnancy Wistar rats.
Navideh Haghnavaz, Faezeh Asghari, Zeynab Sattari, Monire Babaei, Tohied Kazemi,
Volume 18, Issue 4 (winter 2019)
Background & objectives: Breast cancer is one of the most important cancers in women worldwide. Taxol as a chemotherapeutic agent, is used for treatment of breast cancer.The aim of this study was to investigate alterations in the expression of mir-1246 and mir-224 in four breast cancer cell lines after Taxol treatment with the goal of introducing them as a biochemical marker for determining response or resistance of breast cancer to the Taxol therapy.
Methods: In this in vitro study, four breast cancer cell lines including MCF-7, MDA-MB-231, SKBR-3 and BT-474 were cultured in RPMI1640 medium supplemented with 10% FBS and antibiotics. Then, MTT assay was performed to determine IC50 concentration of Taxol. Cells were treated for 24 hours and then RNA extraction and cDNA synthesis were performed. Alterations in the expression level of mir-1246 and mir-224 were quantitated using qRT- PCR.
Results: After treatment with Taxol, the expression level of mir-1246 was significantly up-regulated in two HER2-overexpressing cell lines, BT-474 (113 fold) and SKBR-3 (1.4 fold), and down-regulated in two HER2-negative cell lines, MCF-7 (45.5 fold) and MDA-MB-231 (7.7 fold). Expression of mir-224 was detected only in two cell lines including SKBR-3 and MDA-MB-231, and was down-regulated after treatment with Taxol (2.1 and 17.2 fold, respectively).
Conclusions: According to the different pattern of alteration in the expression level of mir-1246 in HER2-overexpressing breast cancer cell lines compared to HER2-negative cell lines after treatment with Taxol, this miRNA could be a useful biomarker for responsiveness to Taxol in different types of HER2-positive and -negative breast cancers.