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Showing 3 results for Imani Foolad

Abbasali Imani Foolad , Zahra Rostami , Reza Shapouri,
Volume 10, Issue 3 (autumn 2010)
Abstract

  Background and Objectives: Detection of TEM and SHV genes in ESBL producing Pseudomonas aeruginosa and their antimicrobial resistance pattern can provide useful information about the epidemiology and risk factors of associated infections. In this study we determined the antimicrobial susceptibility pattern and prevalence of ESBLs in clinical isolates of Pseudomonas aeruginosa by phenotypic and genotypic methods.

  Methods: In this analytic-descriptive study, 110 Pseudomonas aeruginosa strains isolated from different clinical specimens were used. The pattern of antimicrobial resistance was determined by disk diffusion (Kirby-buer) method. The ESBL production was determined by combination disk method using disks containing ceftazidim and cefotaxim alone and in combination with Clavulanic acid. SHV and TEM types of ESBL producing genes was detected by PCR.

  Results: In this study Co-trimoxazole and Amoxicilin with 96.4% and 92.7% and Amikacin with 17.3% showed the highest and lowest resistance against isolates respectively. According to PCR results 37.5% and 12.5% of isolate were carried SHV and TEM genes respectively and 12.5% of isolate were carried both the SHV and TEM genes.

  Conclusion: According to the results most of the isolates are drug resistant and among the ESBL producing strains the frequency of SHV type is higher than TEM . The isolate ceftazidim resistance was contains SHV (37.5%) and TEM gene (12.5%), that showed SHV and TEM genes play more important role in create of ceftazidim resistance than cefotaxim resistance.


Abbasali Imani Foolad, Maryam Hosainzadeh, Seiyed Fazlollah Mousavi ,
Volume 11, Issue 1 (spring 2011)
Abstract

  Background & Objectives: Pseudomonas aeruginosa is a Gram-negative and aerobic bacterium. Exotoxin A is one of the important toxins produced by the bacterium and it is the main cause of mortality. About 90% of P. aeruginosa strains produce this toxin. Biofilm is a functional consortium of microorganisms attached to the body surfaces and bacteria are embedded in extracellular polymeric substances produced by the microorganisms. This bacterium is nontoxic in the planktonic form, but as a biofilm is highly toxic. In this study, we examined the association between the presence of exo-A gene and antibiotic resistance patterns with biofilm formation in Pseudomonas aeruginosa strains.

  Methods: In this study 110 strains of Pseudomonas aeruginosa isolated from various infections with defined antibiotic resistance patterns were used. The PCR method was used to detect the presence or absence of Exotoxin A gene (exo-A). Ability of biofilm formation was evaluated by spectrophotometry. Association between exo-A gene and antibiotic resistance patterns with biofilms formation was analyzed statistically by Fishers and Chi-square tests.

  Results: exo-A gene was detected in 93 strains (84.5%). Sixty two strains were multidrug resistant and they produced broad spectrum beta-lactamase enzyme. Results showed that, exo-A positive strains had significantly higher ability to biofilm formation in comparison with exo-A negative strains (p<0.05). Also the biofilm formation was significantly higher in multidrug resistant and ESBL producing strains (p<0.05).

  Conclusion: The results of this study indicate that there is a significant association between exo-A gene as well as antibiotic resistance pattern and ESBl producing with biofilm formation in Pseudomonas aeruginosa. Because of importance of biofilms in the pathogenesis of this bacterium, our study could open a new window for investigation of the molecular processes involved in the formation of biofilms.


Maryam Chavoshi Frooshani , Abbasali Imani Fooladi , Sara Saadatmand,
Volume 11, Issue 3 (autumn 2011)
Abstract

  Background & Objectives: Escherishia coli O157:H7 is one of the most important diarrhea causing agents in developing countries . Using antibiotics cause adverse effects as promoting emergence of antibiotic resistance, fading the microflora of intestine and enhancement of verotoxin (VTEC) production by this bacterium. So, a modern treatment protocol is needed for treatment of infections caused by this bacterium. In this study, Lactobacillus casei (L. casei) was isolated from yogurt and antibacterial effects of bacterial cell debris and its culture supernatant were tested against E.coli O157:H7.

  Methods: Several different samples of yoghurt were cultured in MRS agar in anaerobic conditions at 37 ºC. L. casei was identified by common microbiological and molecular methods. Antimicrobial effects of bacterial cell debris and its culture supernatant were tested against E. coli O157:H7 by using Agar Well Diffusion (AWD) and Broth macrodilution methods. In addition, standard growth curves of pathogenic bacterium and L. casei were obtained by turbidometery and colony count procedures. The MIC (Minimum Inhibitory Concentration) and MBC (Minimum Bactericidal Concentration) of supernatant originated from culture of L. casei were determinded. The stability of antimicrobial effects of the supernatant in different conditions of pH and temperature were studied.

  Results: Lactobacillus casei was isolated from two different samples of yoghurts, and confirmed by phenotypic and genotypic methods. The results showed that antimicrobial effects of culture supernatant were stable at 56, 70, 80and 100 ºC for 30 and 60 minutes. Furthermore, they were stable in pH of 3, 7 and 10. The MIC and MBC of supernatants were 1:16 and 1:8 respectively.

  Conclusion: According to the results of this study, culture supernatant of L. casei can be used as a biological preservative in food industries. Also due to antimicrobial effect of L. casei, it can be used in treatment of diseases associated with E. coli O157:H7.



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مجله دانشگاه علوم پزشکی اردبیل Journal of Ardabil University of Medical Sciences
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