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Showing 3 results for Bohlooli
Shaban Alizadeh , Shahab Bohlooli , Ali Abedi , Seiyed Hadi Mousavi , Behzad Jafazadeh, Norooz Hamrang , Ali Imani,
Volume 10, Issue 4 (winter 2010)
Background & Objectives: Leptin is a hormone secreted from adipocyte tissue with established role in the differentiation and proliferation of hematopoietic cells. This hormone has major impact on fat metabolism. LIF is a pleiotropic cytokine with extensive hematopoietic, neuronal, and endocrine actions. LIF and IL-6 are leading to decreased level of leptin by activating signaling via their own receptors. Body mass index (BMI) has a direct connection with the leptin. It seems that Hb and HCT levels are also implicated in disease prognosis. This study was conducted to evaluate leptin, LIF and IL-6 serum levels and also to measure the amounts of BMI, Hb and HCT in lymphoid leukemia patients .
Methods: The study was carried out on 30 leukemia patients (15 cases ALL and 15 cases CLL). Fifteen healthy subjects were considered as control. Serum levels of leptin, LIF and IL-6 were measured by ELISA. BMI was calculated by statistical formula. The amount of Hb and HCT were measured by cell counter. Data was analyzed by SPSS software. Statistical differences between groups were assessed by t test, and p<0.05 was considered significant.
Results: Leptin serum level showed a decrease in ALL patients (p<0.002), but there was an increase in CLL patients when comparing with control group (p<0.003). BMI and serum levels of leptin, LIF and IL-6 were showed a significant decrease in ALL patients in comparison with control group (p<0.05). Although, LIF serum levels and BMI in CLL patients showed a decrease, a significant increase in leptin serum level was observed (p<0.05). A decrease in IL-6 level was also observed which was not significant. The relation between BMI and leptin serum level in ALL and CLL patients were not significant, nevertheless it was significant in control group (p<0.05). Hb and HCT levels in both ALL and CLL patients showed a significant decrease (p <0.05).
Conclusion: Findings on serum levels of LIF, IL-6, Leptin, Hb and HCT and also its relations with BMI in ALL and CLL patients suggest that, these factors may have important role in physiopathology of lymphoid leukemia.
Reza Alipanahmogadam , Mohammad Mazani , Abbas Naghizadeh Baghi, Ali Nemati, Mojtaba Amani , Bahman Bashardoost, Amir Mansour Vatankhah , Shahab Bohlooli,
Volume 11, Issue 3 (autumn 2011)
Background & Objectives: Several evidences show that oxidative stress is high among patients with hemodialysis . This study was aimed to investigate the oxidative stress and serum level of zinc and copper in patients with non-diabetic hemodialysis .
Methods: In a case-control study, 60 patients with non-diabetic hemdialysis referred to hemodialysis section, Ardabil Bou-Ali hospital, and 60 healthy individuals, matched by age and sex, were selected. In each group, lipid profile, blood glucose, creatinine, urea, total protein, albumin, zinc, copper, malondialdehyde, total antioxidant capacity, activity of glutathione peroxidase and superoxide dismutase were measured. The results were analyzed statistically by using Pearson correlation coefficient and Student-t test.
Results: The amount of zinc, blood sugar, cholesterol, triglyceride, total protein, albumin, high density lipoprotein-C and low density lipoprotein-C were significantly lower in patients with hemodialysis in comparison with those in normal individuals. T he activity of superoxide dismutase, glutathione peroxidase in red blood cells and serum total antioxidant capacity were significantly high in patients in comparison with those in control group. However, serum concentrations of copper and malondialdehyde in two groups were not statistically different.
Conclusion : The results of this study show that increase of antioxidant enzymes activity reduces the oxidative stress status in patients with non-diabetic hemodialysis . Zinc deficiency was also observed in these patients.
Keyvan Amirshahrokhi , Shahab Bohlooli , Mohammad Yousefi ,
Volume 18, Issue 3 (autumn 2018)
Background & objectives: The purpose of this study was to show the dose response relationship of anti-inflammatory effect of methylsulfonylmethane (MSM) on carrageenan induced rat paw edema as an acute model of inflammation.
Methods: A total of 54 male, Sprague-Dawley rats, weighing 180-190 g, were used. One hundred, 200, 400, 800 and 1200 mg/kg of MSM were administered intraperitoneally to the rats 30 minutes before induction of paw edema with injection of 0.1% carrageenan. Diclofenac was used as a control drug. Rats were divided into three groups: MSM, diclofenac and normal saline, and their paw tissue were collected for the study of inflammatory and oxidative markers (MDA, GSH, TNF-α and IL-1 β). The relationship between the different concentrations of MSM and decrease in rat paw edema was calculated using a simple Emax model.
Results: the ED50 value for effect of MSM on carrageenan induced rat paw edema was 193±9.7 mg/kg. A significant reduction in paw edema following administration of MSM at 200, 400, 800 and 1200 mg/kg was observed, but statistical analysis did not reveal any significant reduction in paw edema after administration of 100 mg/kg. MSM did not show statistically significant difference from control group in tissue level of GSH, but it was able to decrease MDA level significantly. MSM was able to significantly alleviate IL-1 β and TNF-alpha tissue levels.
Conclusion: The recommended anti-inflammatory dosing range of MSM is 200-800 mg/kg for pharmacological studies in rats and the average appropriate dose is 400 mg/kg. Also, it seems that anti-inflammatory effect of MSM is more profound than its anti-oxidant effects.