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Showing 5 results for Aslani
Ehya Garveriani , Mohammad Mahdi Aslani , Shahram Habibzadeh , Afshin Fathi ,
Volume 7, Issue 4 (Winter 2007)
Background & Objective: Yersinia spp is from Enterobacteriacea family. The members of this group are all gram negative bacilli or cocobacili and non-spore and negative heme-oxidase which casuses a vast spectrum of diseases in humans the commonest one of which is gastroenteritis. Other diseases and clinical syndromes are septicemia, mesenteric lymphadenitis, Appendicitis, pharyngitis and rarely Reiter's syndrome. Due to frequent reports of febrile gastro-enteritis from Health centers of Ardebil, the probability of gastroenteritis resulted from Yersinia in this cold province is predictable. Thus this study was done to determine the role of Yersinia spp in the diarrhea of children under 5 in the cold seasons of the year in Ardebil.
Methods: Stool samples from 490 children under 5 with diarrhea in a 7-month period were collected, and transferred in carry-Blair to the laboratory. Stool samples were cultured on Mac-Conkey agar and Salmonella-Shigella agar and Yersinia selective agar, TCBS agar and selenate agar. After adequate incubation, the growing colonies were identified according to the standard biochemical methods.
Results: of 490 samples, only 405 were suitable to be cultured of which 9.38% pathogenic bacteria were isolated. The frequency distribution of the separated bacteria were as follows Y.enterocolitica, 13 species vibrio, 11 species Enteropathogenic E.coli, 6 species Salmonella, 5 species Shigella, 2 species and plesiomonas, one species.
Conclusion: The findings of this study are the indicator of the role of Yersinia spp in diarrhea in the cold seasons of the year in the cold and mountainous province of Ardebil. On the other hand, the frequency of isolated vibria was due to simultaneous cholera epidemy in this area.
Mohammadyousef Alikhani , Mohammad Mahdi Aslani , Hadi Peeri Dogaheh , Mohammadhosein Dehghan ,
Volume 8, Issue 2 (Summer 2008)
Background & Objective: Tuberculosis is more prevalent in developing countries and death from tuberculosis meningitis is strongly associated with delays in diagnosis and treatment. Polymerase chain reaction (PCR) has been incorporated as a diagnostic tool for the diagnosis of tuberculosis. The rapid results and greater sensitivity compared to traditional microbiological methods makes PCR a suitable technique in tuberculosis, especially in tuberculosis meningitis, when diagnosis is difficult or when rapid diagnosis is needed. However, the possibility of false positive and false negative results must be considered. The aim of this study was to compare the conventional bacteriology (culture Ziehl- Neelsen staining) with polymerase chain reaction (PCR) technique for rapid diagnosis of tuberculosis meningitis.
Methods: This study included 25 clinically diagnosed patients that were suspected to have tuberculosis meningitis and 10 other bacterial or viral meningitis patients were investigated. DNA was extracted from CSF and the NESTED PCR using specific primers were done.
Results: In 25 samples, Mycobacterium tuberculosis DNA was detected in 9 (36%) by PCR, 2(8%) and 1(4%) with culture and direct smear was obtained, respectively. whereas no DNA bands were detected in patient with the other 10 meningitis. The entire procedure was repeated and the same result was obtained.
Conclusion: The findings of this study indicated that PCR is a powerful method for rapid and sensitive diagnosis of tuberculosis meningitis. In a way that it decreases obtaining the results from several weeks in bacteriological methods to one to two days, especially in smear negative patients. This is very important in tuberculosis meningitis because it is a medical urgency and needs rapid diagnosis and early treatment.
Moslem Abbasi , Parviz Porzoor, Kazem Moazedi , Taher Aslani ,
Volume 15, Issue 1 (spring 2015)
Background & objectives: Patients with bulimia nervosa may have impaired mental image of their body and fear of weight gain. The aim of current research is study the effectiveness of acceptance and commitment therapy on improving body image of female students with bulimia nervosa.
Methods: The design of current study is as quasi-experiment research with pre-test and post-test with control group . Statistical population consists all 12-16 years old female students with bulimia nervosa of Ardabil city in the 2013-13 academic years. In order to select the sample, at first 400 students were selected by multi stage cluster sampling method. Then between the students with bulimia nervosa, 40 subjects were selected randomly and were put in two experimental and control groups. The experimental group participated in 8 sessions of acceptance and commitment based training. Thecontrol group received no intervention. The gathered data were analyzed using multivariate analysis of covariance (MANCOVA).
Results: The results show that acceptance and commitment therapy improved body image and reduced the symptoms of bulimia nervosa subjects of experimental group in comparison with the control group in the post-test (P<0.001).
Conclusions: The results indicated that acceptance and commitment therapy by therapeutic strategies, improved body image of female students with bulimia nervosa. Thus, interventions based on this approach in schools for students lead to decreasing the psychological problems
Rana Keyhanmanesh, Mohammad Reza Alipour, Hasan Ghobadi, Mahdi Ahmadi, Alireza Moradi, Mohammadreza Aslani,
Volume 18, Issue 4 (winter 2019)
Background & objectives: Epidemiological and clinical studies have shown a close relationship between asthma and obesity. The present study examined the effect of obesity on the airway response to methacholine and the number of inflammatory cells in the bronchoalveolar fluid of ovalbumin-sensitized male rats.
Methods: Twenty-four male Wistar rats were divided into 4 groups: normal diet (C+ND), OVA‐sensitized with the normal diet (S+ND), high-fat diet (C+HFD) and OVA‐sensitized with high‐fat diet (S+HFD). All animals were fed for 8 weeks with standard diet or high-fat diet, and then were sensitized with ovalbumin or normal saline for another 4 weeks while receiving the designed regimens. At the end of the study, the number of inflammatory cells in bronchoalveolar fluid (BALF) and tracheal responsiveness to methacholine were examined.
Results: In diet-induced obesity groups, weight and obesity indices increased (p<0.05 to p<0.001). The results also showed that tracheal responsiveness to methacholine in S+HFD group compared to S+ND group, was significantly increased (p<0.05). In addition, the number of inflammatory cells in the BAL, in the S+HFD group was higher than other groups (p<0.001).
Conclusion: the results of this study suggest that the response of the airways to methacholine and the number of inflammatory cells are increased in obese-asthmatic male rats.
Mohammad-Reza Aslani, Elham Gholizadeh, Hadi Ghobadi, Afshan Sharghi, Hassan Ghobadi,
Volume 19, Issue 1 (spring 2019)
Background & objectives: Chronic obstructive pulmonary disease (COPD) is characterized by irreversible airflow limitation and systemic inflammation which is also associated with pulmonary and extra-pulmonary complications. COPD Diagnostic Questionnaire (CDQ) is a valid questionnaire that is used to identify patients with COPD. The purpose of this study was to determine the relationship between the severity of airflow limitation and the CDQ score in COPD patients.
Methods: In this study, 100 patients with COPD and smoking history were included. The diagnosis of COPD was established based on American Thoracic Society (ATS) guidelines: cough and expulsion of phlegm, chronic dyspnea, obstructive spirometric pattern (forced expiratory volume in one second (FEV1)/forced vital capacity (FVC) <70%). Disease severity was classified according to GOLD criteria. After obtaining consent, demographic data, history of smoking, the exacerbation rate in the past year, arterial oxygen saturation (Spo2) and spirometer variables were recorded. Then, CDQ and Modified Medical Research Council (MMRC) dyspnea score were completed. Also, the associations of the CDQ score with FEV1% predicted and clinical parameters of patients were tested.
Results: All studied patients were male. There was a significant relationship between CDQ score with FEV1% (p<0.001), GOLD stage (p<0.01), Spo2 (p<0.01), exacerbation rate (p<0.01) and severity of dyspnea according to MMRC dyspnea scale (p<0.01).
Conclusion: The CDQ can be used to determine airflow limitation, severity of dyspnea and exacerbation rate in COPD patients. Therefore, it is recommended that this questionnaire can be used in primary health centers for identifying and referring of COPD patients.