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Showing 6 results for Asgharzadeh

Tajaddin Akbarzadeh Khiavi , Mohammadreza Nahaei , Ahmad Rahmati , Mohammad Asgharzadeh, Javid Sadegi ,
Volume 7, Issue 1 (spring 2007)

  Background and Aims: Staphylococcus aureus as aGram- positive coccus causes a variety of infections in humans. It is one of the infectious agents in hemodialysis patients. Those patients who carry this organism at their nose are exposed to infection and possible morbidity and mortality due to this bacterium. Resistance to antibiotics in staphylococci is increasing. Resistance development is due to mutation and by plasmid DNA transmission. The aim of this study was to determine plasmid profile and antibiotic resistance of Staphylococcus aureus strains isolated from nasal carriers in dialysis patients in Imam Khomeini Medical Center. Susceptibility testing to antibiotics, plasmid extraction and analysis and epidemiologic relationship of these isolates were investigated.

  Methods: In this study nasal specimens of 107 patients in dialysis ward of Imam Khomeini Medical Center were collected and cultured on blood agar plates. The colonies were identified as S.aureus strains. The susceptibility of 50 strains isolated from the patients against 12 antibiotics were tested using Kirby- Bauer standard method. A standard S.aureus strain (ATCC29213) was used to control quality of antibiotic discs. The isolates were cultured on LB medium and plasmid DNAs were extracted and electrophoresed on agarose gel using Parisi et al method.

  Results : The results of resistance rate against 12 used antibiotics were as follows: resistance of the strains against gentamicin, oxacillin, neomycin, clindamycin, erythromycin, cotrimoxazole, choloramphenicole, tetracycline, and ciprofloxacin were 20%, 28%, 30%, 26%, 30%, 44%, 32%, 36%, and 10%, respectively. All of the strains were resistant to amoxycillin and penicillin and none of them were resistant to vancomycin. Of 50 S. aureus strains, only 27 strains contained plasmid DNA. Most of the strains revealed a big plasmid. Plasmid profiles of the strains will be presented.

  Discussion: Our results showed that there was a close relationship between high resistance to antibiotics and presence of plasmids in S. aureus strains. Similarities among resistance to antibiotics and plasmid profiles in our strains isolated from the same ward showed that these strains were from the same sources and indicated a unique clonal possibility. The resistance to antibiotics of the strains lacking plasmids could be from choromosomal resistance

Behnam Mohammadi Ghaleh Bin , Esmaeil Fallah, Mohammad Asgharzadeh, Abdolhasan Kazemi ,
Volume 7, Issue 2 (Summer 2007)

  Background & Objectives: Cryptosporidium is a coccidian protozoan parasite. This organism is one of the main causes of severe, long-time and life-threatening diarrhea in immunocompromised persons. It is also among the most prevalent diarrheal agents in children. Cryptosporidial epidemics occur after consumption of water which is contaminated by oosit species of cryptosporidium. Water is usually contaminated by animal feces or by drainage of waste water into drinking water resources.

  Methods: In this study, from ten regions 200 water samples were collected, filtered by 1.2 micron papers and then positive samples were identified in terms of cryptosporidium using PCR method. Finally the related species were detected by RFLP method.

  Results: Nested-PCR showed 8 samples were positive for cryptosporidium that according to RFLP of PCR products 5 samples belonged to cryptosporidium andersony, 2 samples belonged to cryptosporidium parvum bovine genotype and 1 sample belonged to cryptosporidium pig genotype.

  Conclusion: Since Cryptoridium andersony and cryptosporidium parvum bovine genotype are the common species in animals and cryptospovidium swiss is seen in wild animals (pigs and boars), it so we conclude that animal reservoirs have the main role in the contamination of related water resources in this region.

Hosein Khadem Haghighian , Alireza Farsad Naimi, Bahram Pourghassem Gargari , Akbar Ali-Asgharzadeh , Ali Nemati ,
Volume 10, Issue 4 (winter 2010)

 Background & Objectives: Different types of diets and several chemical and herbal drugs are used for decreasing the fasting blood glucose, glycosylated hemoglobin and insulin resistance in type II diabetic patients. New herbal medicines including cinnamon have been considered for controlling diabetes. Since few reports have been presented in other countries and many studies have been done in animal models in laboratory condition, this study was aimed to investigate cinnamon supplementation effects on fasting blood glucose, glycosylated hemoglobin and insulin resistance among type II diabetic patients .

 Methods: In a clinical trial study, 60 male and female patients with type II diabetes mellitus (30 patients in control and 30 patients in treatment group) were selected in Tabriz city, during 1388. The intervention group received 1.5 g of cinnamon (as a capsule containing 500 mg powder, three times daily) for 60 days and control group received placebo. Blood samples obtained from patients to determine the levels of fasting blood glucose, the glycosylated hemoglobin and insulin, before and after cinnamon consumption. Insulin resistance was measured by HOMA score and data were expressed as Mean ± SD and analyzed statistically by Student t-test. p<0.05 was considered as significant .

 Results: After 60 days, the fasting blood glucose levels, the glycosylated hemoglobin and the insulin resistance decreased significantly in the intervention group compared to controls (p<0.05). There was no significant change in the fasting blood glucose levels, the glycosylated hemoglobin and the insulin resistance in the control group at the end of 60 days.

 Conclusion: This study showed the consumption of cinnamon can be useful in the fasting blood glucose, the glycosylated hemoglobin and the insulin resistance control among type II diabetic patients .

Mohammad Reza Asgharzadeh, Seyed Mohammad Atyabi, Hossein Khan Ahmad Shahrza, Somaye Asgharzadeh, Akbar Jalili, Reza Ahanghari Cohan, Davod Nouri Inanlo,
Volume 13, Issue 2 (summer 2013)

  Background & Objective: Gene therapy and administration of recombinant protein are common approach in treatment of genetic disorders. But many obstacles including frequent administration of desired gene, random integration into the host genome and low expression of protein encourage scientists to design an episome which remains in high copy number in eukaryotic cells and produces desired protein in suitable manner by viral proteins. The aim of this study is designing and construction of a plasmid containing mutated large T antigen of SV40 to develop a safe vector with high replication.

  Methods: Suitable mutant for creating large T antigen was analyzed by MODELLER software and finally appropriate structures were selected. Target mutation was created in the nucleotide sequence of large T antigen by PCR method. Mutated large T antigen gene was cloned in to the IRES2-EGFP. All clones were analyzed by PCR, restriction analysis and sequencing. HEK293 and CHO cell lines were transfected by final construct and transfected cells were observed by fluorescent microscope for 40 days. Plasmid and genomic DNA were extracted from remained cells and overlap PCRs performed on them to confirm their circularity.

  Result: This plasmid, containing a mutated large T antigen of SV40, can be replicated in eukaryotic cells and then can be used in gene therapy and recombinant protein production with high safety.

  Conclusion: The results of PCR, restriction analysis and sequencing confirm the authenticity of construct. The transfection of HEK293 and CHO cell lines showed replication of constructed plasmid in them.

Jalil Rashedi, Mohammad Asgharzadeh, Seyed Reza Moaddab, Mojtaba Amani, Mohammad Mazani,
Volume 13, Issue 4 (Winter 2013)

  Background & Objectives: It is estimated that one third of the world’s population is infected by M. tuberculosis. Because of differences in immune system activity against the invasive microorganisms, the disease is developed only among 10% of them. Vitamin D metabolism and its receptor activity are important factors in human native immune system against tuberculosis. In the present study we investigated ApaI polymorphism of vitamin D receptor (VDR) gene and association with susceptibility to tuberculosis.

  Method: This study was performed on 84 cases with tuberclosis (male =50, female =34) and 90 controls (male =49, female = 41). DNA was extracted from cases and controls leucocytes and elected sequences amplified in polymerase chain reaction (PCR) procedure by using specific primers. ApaI polymorphism of VDR gene evaluated by RFLP technique on PCR products. Finally statistical analysis performed using Chi- square to compare genotype frequencies between cases and controls.

  Results: In case and control groups, AA genotype frequency were 34.5% and 33.3% respectively (OR=0.905, 95% CI 0.469-1.747, p = 0.766) and a genotype frequency in patients and control group were 15.47% and 13.3% respectively (OR=0.808, 95% CI 0.333 –1.961, p=0.637).

  Conclusion: In the present study we could not find any significant relationship between genotype frequency of ApaI (A/a) polymorphism in VDR gene and susceptibility to tuberculosis.

Roqiyeh Nouri, Mohammad Ahangarzadeh Rezaee , Alka Hasani, Mohammad Aghazadeh, Mohammad Asgharzadeh, Morteza Ghojazadeh,
Volume 16, Issue 2 (summer 2016)

Background & objectives: Fluoroquinolones have important role in treatment of P. aeruginosa infections. The main mechanism of fluoroquinolones resistance in P. aeruginosa is mutations in the quinolone-resistance-determining region (QRDR) of gyrA and parC genes. The aim of this study was to investigate the role of these mutations in ciprofloxacin resistance in different clinical isolates of P. aeruginosa.

Methods: A total of 75 clinical P. aeruginosa isolates were collected from different university-affiliated hospitals in Tabriz. Minimum inhibitory concentrations (MICs) of ciprofloxacin were evaluated by Etest assay. DNA sequences of the QRDR of gyrA and parC were determined by dideoxy chain termination method.

Results: From 75 isolates, 77.33% were resistant to ciprofloxacin. No amino acid changes were detected in gyrA or parC genes of the ciprofloxacin susceptible isolates. Thr-83→Ile substitution in gyrA was observed in all ciprofloxacin resistant isolates. About 90% of them had Ser-87→Leu substitution in parC. Geometric mean MICs of ciprofloxacin were different for various clinical isolates of P. aeruginosa which had the same situation in type and location of gyrA and parC mutations. Moreover, the geometric mean MIC in isolates from urine was significantly (p<0.05) higher than isolates from tracheal aspirates.

Conclusion: Mutations in gyrA and parC genes are the major mechanisms for ciprofloxacin resistance in clinical isolates of P. aeruginosa. Moreover, the role of different effective factors in fluoroquinolone resistance can be different in various clinical isolates of P. aeruginosa.

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مجله دانشگاه علوم پزشکی اردبیل Journal of Ardabil University of Medical Sciences
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