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Showing 6 results for Asghari

Ali Hosein Khani , Magid Ali Asghari , Seid Yoosof Hoseini, Naiere Amini Sani ,
Volume 6, Issue 1 (spring 2006)

  Background & Objectives: Benign prostatic hyperplasia is one of the most common diseases in males and Transurethral Resection of Prostate (TUR-P) is a standard method for its surgery. Distilled water is used to clean the field of operation from blood and debris. The overabsorption of this washing fluid can lead to hemodynamic disorders and neurological complications called TUR-P syndrome. Because of hemolysis and ATN, most of the urologists, nowadays, prefer to use non-hemolytic solutions such as cytol and glycine 1.5%. Distilled water is widely used in Iran. Replacing this water with a nonhemolytic and hypoosmolar solution with reasonable price such as manitol 3% seems reasonable. This study was carried out to campare manitol 3% with distilled water during TUR-P.

 Methods: In a double-blind randomized clinical trial, we studied 78 patients with BPH who were admitted to Shahid Moddares hospital in Tehran for TUR-P. These patients were allocated to disstiled water group and manitol 3% group. Na, K, Cr, BUN, CBC, U/A, U/C, 24 hr urine volume and creatinine were checked preoperatively to determine GFR, Na, K, BUN and CBC were checked right after, 4 hours after and on the morning after the operation. Clinical signs of TUR-P syndrome were recorded in the patients as bradycardia, hypertension and neurologic sign.

  Results : Mean age was 68.6 in distilled water group and 66.4 in the manitol group. Mean weight of resected tissues, mean volume of the solution used and mean resection time were 19.8 gr, 19 litre, 50.8 minutes respectively in distilled water group and 20.2 gr, 20.3 litre and 51 minutes in the manitol 3% group. These differences were not statistically significant. The difference between decrease in serum Na and serum osmolality was not significant in two groups, however, hemolysis rate in two groups was statistically significant (p<0.01). The incidence rate of TUR-P syndrome was 34% in the distilled water group and 18% in manitol group. This difference was not statistically significant. Postoperative creatinine increase was 0.625 mg/dl in distilled water group and 0.04 mg/dl in manitol group. This was not statistically significant.

  Conclusion: Regarding the role of distilled water in hemolysis and ATN and due to the unavailability of non – hemolytic solutions such as glycine and cytol in Iran, the use of manitol 3% soluion which is a cost-effective, non hemolytic and hypoosmolar solution is recommended.

Maryam Nematollahzade , Raziyeh Maasoumi , Meynoor Lamyian, Mohammad Asghari Jafarabadi ,
Volume 10, Issue 3 (autumn 2010)

  Background & Objectives: Pregnancy accompanies with physical and psychological alternation during woman's life. These changes effluence the overall dimensions of couple’s life especially sexual relation. The aim of this study was to investigate the women’s attitude and sexual function during pregnancy.

  Methods: This descriptive-analytical study was conducted among 329 healthy pregnant women aged 15-45 years. The attitude and sexual function of the cases were studied based on their gestational age (the last fourth weeks of each pregnancy trimester) in three groups. Data were collected using an instrument. The instrument has three parts including demographic characteristics, attitude assessment and Arizona Sexual Scale Experience (ASEX). One way ANOVA, Post hoc, chi square and Spearman correlation were used for data analysis and the differences were considered to be significant at the p<0.05 level.

  Results: The results showed that the score of sexual dysfunction, sexual desire disorder and orgasmic disorder increase with gestational age development. Statistical analysis demonstrated a significant relation between total score of sexual function disorder and decrease of sexual attraction in all three trimesters (p=0.01, p=0.04, p=0.03 respectively) and fear of prematurity in the second trimester (p=0.01).

  Conclusion: According to the results sexual function disorder has a high frequency in pregnancy. The findings emphasize the need for educational programs to improve the Sexual functions during prenatal care.

Maryam Rafraf, Elaheh Mohammadi, Laya Farzadi, Mohammad Asghari-Jafarabadi, Siyamak Sabour,
Volume 12, Issue 4 (winter 2012)

  Background & Objectives: Polycystic ovary syndrome (PCOS) is one of the major endocrine disorders among females. PCOS is associated with development of type 2 diabetes and cardiovascular disease. The objectives of this study were to determine the effects of omega-3 fatty acids on glycemic status and high sensitive C-reactive protein in women with PCOS.

  Methods: This double-blind randomized controlled clinical trial was conducted on 61 PCOS patients. Subjects in ω-3 fatty acids (n=30) and placebo (n=31) groups take 4 ω-3 fatty acids capsules (each one contained 180 mg eicosapentaenoic acid and 120 mg docosahexanoic acid) or placebo daily for 8 weeks. Anthropometric and biochemical measurments and food intakes were assessed at the beginning and at the end of the study.

  Results: ω-3 fatty acid supplementation caused significant decrease in serum levels of glucose, insulin (p < 0.001 for both) and insulin resistance (p = 0.002) at the end of the study in comparision with baseline values. This was also statistically significant compared to the placebo group (p < 0.001, p = 0.002 and p = 0.001, respectively). No statistically significant differences in serum levels of high sensitive C-reactive protein were obsereved in any of groups at the end of the study (p > 0.05).

  Conclusion: ω-3 fatty acid supplementation had some beneficial effects on glycemic status of PCOS patients and may be useful in prevention and control of metabolic conmplication of this syndrome.

Hamideh Asghari , Nematollah Gheibi , Kourosh Goodarzvand Chegini, Mahdi Sahmani , Darioush Ilghari ,
Volume 14, Issue 2 (summer 2014)

   Background & objectives: Calprotectin, S100A8 and S100A9 are involved in important processes including cell signaling and regulation of inflammatory responses. In this study, recombinant expression, purification and structural characterization of S100A8 and S100A9 were accomplished.

  Methods : In this experimental study, pET15b was used as vector of human S100A8 and S100A9 coding sequences, hosted by E.coli BL21 (DE3). Gene expression and purification attempts were evaluated using SDS-PAGE. Protein purification was accomplished using Ni-NTA resin based on its affinity for His-tag present on recombinant proteins. Tertiary structure of proteins were evaluated using spectrofluorimetry.

  Results : The subunits were over expressed 3-4 hours following induction at 37 °C. S100A9 was expressed mainly as inclusion body while S100A8 was found to be expressed mainly as a soluble protein. Purification of S100A8 and S100A9 was achieved at 100 mM imidazole. Spectroscopic studies showed that the amino acid tryptophan is in the internal structures and is less exposed to the aqueous environment.

  Conclusion : In this study, a recombinant S100A8 and S100A9 subunits were expressed and purified and also their structures were confirmed.


Behnam Mohammadi-Ghalehbin , Kimyia Rahimi , Ali Pezeshki , Firooz Amani , Peyman Azghani , Soltan Asghari , Elham Raeisi ,
Volume 15, Issue 1 (spring 2015)

  Background & objectives: Trichomonas vaginalis is a protozoan parasite lives and also causes symptoms in urogenital system of human. Trichomoniasis is the widest spread infection which is transmitted through sexual contact and more than 170 million people are a ffected with Trichomonas vaginalis in the world. Trichomonas vaginalis in women causes pelvic inflammatory disease, increase in the risk of fallopian tube dependent infertility, ectopic pregnancy, preterm labor, the birth of low weight infants and the increase in the possibility of HIV transmission. Considering the importance of Trichomonas vaginalis infection in pregnant women and lack of data from Ardabil city, this study was performed to identify this infection in pregnant women referred to health and medical centers of Ardabil city.

  Methods: Vaginal discharge from 500 pregnant women was collected with sterile swap and disposable speculum and examined for Trichomonas vaginalis by direct microscopic examination and cultured in Diamond specific medium. A testimonial and questionnaire were completed for each case and the results analyzed using chi-square test by SPSS statistical software version 19.

  Results: In this study, the culture of samples displayed 12 positive cases (2.4%). Furthermore, Trichomonas vaginalis trophozoites were observed in five cases (1%) with direct microscopic examination. Among 12 positive cases, five respondents (41.7%) were in age range of 16-25 years and seven (58.3%) in 26-35 years old. Among different clinical manifestations there was a significant relation between discharge and the infection.

  Conclusion: The present survey confirmed the presence of Trichomonas vaginalis infection in pregnant women of Ardabil city. Therefore, an effective healthcare program by health authorities for prevention of infection in this group seems to be needed.

Navideh Haghnavaz, Faezeh Asghari, Zeynab Sattari, Monire Babaei, Tohied Kazemi,
Volume 18, Issue 4 (winter 2019)

Background & objectives: Breast cancer is one of the most important cancers in women worldwide. Taxol as a chemotherapeutic agent, is used for treatment of breast cancer.The aim of this study was to investigate alterations in the expression of mir-1246 and mir-224 in four breast cancer cell lines after Taxol treatment with the goal of introducing them as a biochemical marker for determining response or resistance of breast cancer to the Taxol therapy.
Methods: In this in vitro study, four breast cancer cell lines including MCF-7, MDA-MB-231, SKBR-3 and BT-474 were cultured in RPMI1640 medium supplemented with 10% FBS and antibiotics. Then, MTT assay was performed to determine IC50 concentration of Taxol. Cells were treated for 24 hours and then RNA extraction and cDNA synthesis were performed. Alterations in the expression level of mir-1246 and mir-224 were quantitated using qRT- PCR.
Results: After treatment with Taxol, the expression level of mir-1246 was significantly up-regulated in two HER2-overexpressing cell lines, BT-474 (113 fold) and SKBR-3 (1.4 fold), and down-regulated in two HER2-negative cell lines, MCF-7 (45.5 fold) and MDA-MB-231 (7.7 fold). Expression of mir-224 was detected only in two cell lines including SKBR-3 and MDA-MB-231, and was down-regulated after treatment with Taxol (2.1 and 17.2 fold, respectively).
Conclusions: According to the different pattern of alteration in the expression level of mir-1246 in HER2-overexpressing breast cancer cell lines compared to HER2-negative cell lines after treatment with Taxol, this miRNA could be a useful biomarker for responsiveness to Taxol in  different types of HER2-positive and -negative breast cancers.

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مجله دانشگاه علوم پزشکی اردبیل Journal of Ardabil University of Medical Sciences
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