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Mohammadreza Nahaei , Reza Bohloli Khiavi , Mohammad Asgarzadeh, Alka Hasani , Javid Sadeghi, Mohammad Akbari Dibavar ,
Volume 7, Issue 1 (spring 2007)
Background and Objectives: Pseudomonas aeruginosa is a nosocomial pathogen that presents high antibiotic resistance.There are phenotyping and genotyping methods for epidemiologic study of Pseudomonas aeruginosa such as antibiotic resistance pattern and plasmid profile analysis. Plasmid analysis provides useful information concerning the source of the strains and number of clones present in the epidemies. Thus, this study was conducted to evaluate antibiotic and plasmid profiles of P. aeruginosa strains isolated from in-patients of the Sina Medical Centre of Tabriz to clarify epidemyological correlation among isolated strains.
Methods: During 13 months, 135 strains of P. aeruginosa were isolated from different infections in hospitalized patients at Sina Medical Center of Tabriz. Antibiotic susceptibility tests were performed using disc agar diffusion test. For plasmid DNA extraction and detection of open circular bands from supercoiled ones, modified alkaline lysis procedure and two dimensional electrophoresis were used, respectively. Enzymatic digestion of plasmids was carried out by EcoRI and HincII restriction enzymes.
Results: Resistance rates of strains against antibacterial agents were recorded as: Aztreonam (77%), colistin (74%), ceftazidime (69%), pipracillin (67%), ofloxacin (62%), tobramycin (56%), carbenicillin (54%), gentamicin (51%), ciprofloxacin (22%), amikacin (15%), polymixin B (13%) and imipenem (2%). Plasmid profiles of our test strains revealed that only 67 strains harbored plasmid(s). Number of isolated plasmids ranged 1-6 in each strain with molecular mass of 0.5kb-21kb. When the isolated plasmids were digested using restriction endonuclease enzymes (EcoRI and HincII), in 32% of them similar digestion profiles were obtained by EcoRI indicating a unique source for them.
Conclusion : Our findings suggest high antibiotic resistance and plasmid presence in P. aeruginosa strains isolated from different infections, and there were remarkable similarities among isolated plasmids. Since our test strains had been isolated from various wards in a short period of time, the results raise the possibility of unique source for some strains or high prevalence of genetic exchange among P. aeruginosa strains.
Bahram Pourghassem Gargari , Parvin Dehghan, Elham Mirtaheri, Akbar AliAsgarzadeh,
Volume 13, Issue 4 (Winter 2013)
Background & Objectives: Diabetes mellitus is a metabolic disease characterized by hyperglycemia together with biochemical alterations of lipid profile, insulin resistance and inflammation . Considering the high prevalence of hypertension, dyslipidemia and inflammation in type 2 diabetic patients, the aim of the present study was to investigate the effects of inulin on lipid profile, inflammation and blood pressure in women with type 2 diabetes.
Methods: In this controlled, randomized clinical trial, 49 women with type 2 diabetes (fiber intake<30 g/d, BMI=25-35 kg/m2) were assigned to one of two groups. Experimental group (n=24) received 10 g/d inulin and control group (n=25) received 10 g/d maltodextrin for 8 weeks. Dietary intakes, anthropometric measurements, blood pressure, serum lipids and hs-CRP concentrations were measured at the baseline and at the end of the study. Data were analyzed using SPSS software (verision11.5). Paired, independent t-tests and ANCOVA were used to compare quantitative variables.
Results: At the end of study, there was a significant decrease in systolic blood pressure (from 135.7 ± 16.2 to 125.9 ± 7.9 mmHg), total cholesterol ( from 192.5 ± 42.8 to 171.0 ± 39.7 mg/dl), triglyceride ( from 223.3 ± 84.2 to 169.9 ± 65.6 mg/dl) and hs-CRP ( from 7.9 ± 3.0 to 5.3 ± 3.0 mg/l) in inulin group compared with the maltodextrin group (p > 0.04 ). Changes in diastolic blood pressures, LDL-c and HDL-c were not significant in inulin group compared with the maltodextrin group. A significant decrease in systolic, diastolic blood pressures, total cholesterol, triglyceride, LDL-c, hs-CRP and significant increase in HDL-c were observed in inulin group compared to baseline.
Conclusions: Inulin supplementation may improve lipid profile, hs-CRP and blood pressure in women with type 2 diabetes.