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Showing 4 results for Subject: ژنتیک و پزشکی مولکولی
Behzad Baradaran, Saeed Noorolyai ,
Volume 18, Issue 4 (3-2019)
Backgrounds & objectives: Colorectal cancer (CRC) is the third leading cause of cancer death worldwide. Micro RNAs are a group of non-coding small RNAs that inhibit the translation of target mRNA. MiR-146a-5p, as a tumor suppressor, has abnormal expression in many cancers. In this basic research, our goal was to restore the expression level of miR-146a-5p to normal level and to investigate its effect on the expression of the MMP9 gene in HT-29 cells.
Methods: this study evaluates the effect of transfection of miR-146a-5p in HT-29 cell line. At first, the HT-29 cell line from colorectal cancer was cultured in RPMI-1640 culture media and then were transfected with miR-146a-5p using Jet-PEI reagent. qRT-PCR technique was employed to evaluate the expression level of miR-146a-5p and MMP9 genes. The statistical analysis was performed using GraphPad Prism 6 software.
Results: According to the obtained data, the onset of the invasion and metastasis, in particular, at the final stage of colorectal cancer may be related to a reduction in the expression of miR-146a-5p. The results of the qrRT-PCR test showed that by increasing the expression level of miR-146a-5p in HT-29 cells, the expression level of MMP9 gene decreased in the miR-146a-5p transfected group compared to the control group.
Conclusions: According to this study, activation of metastatic pathways was due to the down regulation of miR146a-5p. Accordingly, miR-146a-5p can inhibits migration of these cells through down-regulating the expression of metastasis-related genes. Hence, miR-146a-5p can be a new diagnostic biomarker and new therapeutic target for CRC.
Farhad Salehzadeh, Afshan Sharghi, Atena Moteyagheni, Saeid Hosseini Asl, Mahsa Mottaghi, Sepehr Sarkhanloo,
Volume 19, Issue 1 (4-2019)
Background & objectives: MEFV gene has a major role in Familial Mediterranean Fever (FMF) as an auto-inflammatory disorder. FMF is most often seen in the people of the Mediterranean area. Considering the significant role of the MEFV gene in many rheumatologic diseases and even non-rheumatologic disorders, it is necessary to identify different variations of these mutations in the healthy and normal population of this area.
Methods: 224 healthy people entered into this study. The blood samples were screened for the 12 most common MEFV gene variants according to manufacturer’s instructions. (FMF Strip Assay, Vienna lab, Vienna, Austria)
They filled a questionnaire containing the required information. All patients were initially evaluated for the FMF symptoms and signs in themselves and their first-degree relatives based on clinical criteria. Chi-squared test and t-test were employed for statistical analysis using SPSS ver.24.
Results: Among 224 cases, 113 cases (50.4%) were male, and 111 cases (49.6%) were female. MEFV mutations were detected in 57 patients (25%) of them, 28 cases were male (49.1%) and 29 cases were female (50.9%). The most frequent mutations were E148Q (18.3%, 41cases), followed by P369S (3.1%, 7 cases), V726A (2.2%, 5cases), A744S (1.3%, 3cases), F479L, M694V and R761H (0.8%, each 2 cases), and eventually K695R (0.4%) respectively. Some mutations such as M694I, M680I (G/C), M680I (G/A), I692del were not seen in these samples. There were compound heterozygous mutations of E148Q/P369S, E148Q/V726A, E148Q/P369S, and P369S / F479L in normal population without any findings in favor of FMF.
Conclusion: Twenty-five percent of the normal population of the northwest of Iran carrying a heterozygous variant of the MEFV gene, E148Q (18.3%) as a most common mutation, which can be considered as a normal variant in the healthy population. The presence of M694I, M680I (G/C), M680I (G/A) and I692del mutations in the normal population can be interpreted with cautiously, while particular compound heterozygous mutations can be considered as normal variants.
Mohammad Reza Izadpanah, Ali Saleh Zadeh, Mohammad Zaiefi Zadeh, Mohammad Nik Pasand,
Volume 19, Issue 2 (9-2019)
Background & objectives: Breast cancer is one of the most important causes of cancer deaths especially in developing countries. However, some synthetic compounds such as thiosemicarbazone and its metal derivatives can play a very significant role in reducing the death rate from cancer. The aim of this study was to investigate alterations in the expression of BRCA1, p53 and Bcl-2 genes in breast cancer cell line.
Methods: In this study, the effect of pyrazole derivative of thiosemicarbazone (T) as well as T-bound iron oxide magnetite nanoparticles (T/ Fe3o4 np) were investigated on expression changes of Bcl-2, BRCA1 and p53 in MCF-7 cells. For this purpose, the toxicity of the compounds was first measured using the MTT reaction for 24 hr. Then, the mRNA expression levels were evaluated using real-time PCR reaction.
Results: The results showed a high toxicity for the nano-pyrazole derivative of thiosemicarbazone magnetite so that; the fatality rate for treated thiosemicarbazone magnetite derivative cells was much higher than the compound without any nanoparticle. Also, real-time polymerase chain reaction analysis of gene expression indicated a high effect of the derivative on increasing the expression levels of BRCA1 and p53 genes and also decreasing the Bcl-2 gene expression of Bcl-2.
Conclusion: The use of anticancer compounds like thiosemicarbazone which bound to iron oxide nanoparticles would increase the toxicity of the anticancer compounds along with enhancment of delivery of them with high solubility to target tissue.
Lida Jalali Dizage, Mohammad Reza Nahaei, Javid Sadegi,
Volume 19, Issue 3 (10-2019)
Background & objectives: Urinary tract infection (UTI) is one of the most common types of human infections and Escherichia coli and Klebsiella pneumonia are the main causes of urinary tract infection among the gram negative bacteria. The prevalence of extended-spectrum β-lactamases (ESBLs) among these bacteria and hence resistant strains to β-lactam antibiotics have increased in recent decades. Several types of extended-spectrum β-lactamases, such as TEM, SHV and CTX-M have been identified, which are prominently present in the strains of E. coli and K. pneumoniae. The objective of this study was to determine the prevalence of TEM and SHV genes in E. coli and K. pneumoniae isolates of urinary tract infections by using phenotypic and molecular (PCR) techniques in microbiology laboratory at medical school of Tabriz Islamic Azad University.
Methods: In this cross-sectional descriptive study, 50 isolates of E. coli and 50 isolates of K. pneumoniae collected from urinary tract infections from out-patients in Tabriz. Antibiotic sensitivity patterns of isolates were studied against 14 antibiotics by disk diffusion test (Kirby Bauer) and also confirmatory tests were performed using combined antibiotic tests. Finally TEM and SHV genes were investigated using molecular methods (PCR).
Results: Twenty five isolates (25%) out of 100 bacterial isolates were identified as ESBL-producing isolates of which 13 isolates (26%) were E. coli and 12 isolates (24%) were K. pneumoniae. The TEM and SHV genes were detected in 2% and 4% of E.coli and 0% and 2% of K. pneumoniae isolates, respectively.
Conclusion: The presence of these genes among our isolates confirmed ESBL genes in these medically important bacteria leading to resistance against β-lactam antibiotics which are routinely used in their treatments. The low frequency of the studied genes could be because of the source of our isolates from out-patients which are not generally exposed to antibiotics