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:: Volume 16, Issue 3 (autumn 2016) ::
J Ardabil Univ Med Sci 2016, 16(3): 261-270 Back to browse issues page
Semi-Quantitative Evaluation of KAI1 Gene Expression in Colorectal Cancer and Correlation with Clinical Stage and Progression Factors of the Disease
Shahla Mohammad Ganji , MohammadNaser Molapour, Golamreza Javadi, Eisa Jahanzad
Department of Molecular Medicine, National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran
Abstract:   (3882 Views)

Background & objectives: KAI1 is a tumor suppressor gene and inhibitor of metastasis in a wide range of malignancies. While it is ubiquitously expressed in normal tissues, KAI1 expression subjects to the down regulation in tumors. The present research aims semi-quantitative evaluation of KAI1 mRNA expression in Iranian patients with colorectal cancer (CRC) and correlation between expression levels of KAI1 and stage oftumorigenesis, especially metastasis and invasion of CRC as well as pathologic factors of patients.

Methods: RT-PCR was done by specific primers for KAI1 and β-actin genes on the 80 tumor tissues and 14 normal tissues as fresh samples which obtained from 80 unrelated patients referred to Imam Khomeini Hospital.  

Results: According the results, 51.2% and 48.8% of the sample were on and off for KAI1 gene expression, respectively. As a detail, 97.3% of samples in the stage 3 and 4 and 94.5% of metastatic phases samples showed no expression of this gene. Statistical analysis showed that there is a significant difference of the KAI1 expression between four groups of samples; normal, stage 1, 2 and 3 (p<0.05). Also a significant difference was observed between semi-quantitative KAI1 expression and degree of spread to regional lymph nodes (p=0.02) as well as semi-quantitative KAI1 expression and metastasis (p=0.000001).

Conclusion: A significant difference between semi-quantitative expression of KAI1 and degree of spread to regional lymph nodes (p=0.02) and metastasis (p=0.000001) was observed.

Keywords: Metastasis, Colorectal Cancer, Gene Expression
Full-Text [PDF 164 kb]   (1669 Downloads)    
Type of Study: article | Subject: General
Received: 2015/06/15 | Accepted: 2015/11/26 | Published: 2016/10/1
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Mohammad Ganji S, Molapour M, Javadi G, Jahanzad E. Semi-Quantitative Evaluation of KAI1 Gene Expression in Colorectal Cancer and Correlation with Clinical Stage and Progression Factors of the Disease. J Ardabil Univ Med Sci. 2016; 16 (3) :261-270
URL: http://jarums.arums.ac.ir/article-1-1168-en.html


Volume 16, Issue 3 (autumn 2016) Back to browse issues page
مجله دانشگاه علوم پزشکی اردبیل Journal of Ardabil University of Medical Sciences
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