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:: Volume 15, Issue 2 (summer 2015) ::
J Ardabil Univ Med Sci 2015, 15(2): 189-197 Back to browse issues page
Evaluation of Immunogenicity of Yersinia enterocolitica O:8 Oligopolysaccaride-DiphtheriaeToxoide Conjugate in Mice
SM Rezavian, R Shapouri
Abstract:   (4121 Views)

  Background & objectives: Yersiniosis is created by Yersinia enterocolitica O:8 and causes problems in the world especialy in cold and mild countries. The purpose of this study is to evaluate the immunogenicity of Yersinia enterocolitica O:8 oligopolysaccaride (OPS) conjugate to diphtheria toxoid (DT) as a vaccine candidate.

  Methods : After cultivation of bacteria, the LPS were isolated by modified hot phenol method. Then dialysis and concentration were done and the OPS were extracted by acetic acid 2%. To conjugate with diphtheria toxoid, ADH was used as a spacer molecule and EDAC as a linker. Conjugate was purified by gel filtration. Then 4 groups of female BALB/c mice were selected (15 mice in each group). Injection was performed intraperitoneally in three doses with two weeks interval. Then serum samples were collected and antibody response against OPS was measured by indirect ELISA method for detection of total IgG, IgA, IgM, IgG1, IgG2a, IgG2b and IgG3.

  Results: After second and third doses, OPS-DT recieved group showed significant increase in all types of antibodies titer in anti-OPS in comparison to group that recived nonconjugated OPS. The increase in titer of antibodies was as: OPS-DT>OPS>DT. A remarkable increase was shown in total IgG and IgM titers (with total amount of 3204 and 670, respectively). In IgG1 subclass the amount was 920 and in other subclasses of IgG (IgG3, IgG2a and IgG2b) the amounts were 910, 110, and 99, respectively.

  Conclusion: The results shows that OPS of Yersinia enterocolitica O:8 increases the anti-OPS antibodies in the form of conjugate with diphtheria toxoid and could be considered as an appropriate vaccine candidate.

Keywords: Yersinia enterocolitica O:8, OPS, Conjugate Vaccine, Diphtheria Toxoid
Full-Text [PDF 155 kb]   (1429 Downloads)    
Type of Study: article |
Received: 2014/12/15 | Accepted: 2015/05/26 | Published: 2015/07/1
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Rezavian S, Shapouri R. Evaluation of Immunogenicity of Yersinia enterocolitica O:8 Oligopolysaccaride-DiphtheriaeToxoide Conjugate in Mice. J Ardabil Univ Med Sci. 2015; 15 (2) :189-197
URL: http://jarums.arums.ac.ir/article-1-851-en.html


Volume 15, Issue 2 (summer 2015) Back to browse issues page
مجله دانشگاه علوم پزشکی اردبیل Journal of Ardabil University of Medical Sciences
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