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:: Volume 15, Issue 1 (spring 2015) ::
J Ardabil Univ Med Sci 2015, 15(1): 56-65 Back to browse issues page
Methimazole-Induced Hypothyroidism Enhances HSV-1 Infectivity without Altering Circulating Leukocytes in the Rat
Hadi Feizi, Afagh Moattari, Zahra Amirghofran, Masoumeh Varedi
Abstract:   (5023 Views)

  Backgrounds & objectives: In addition to their genomic actions, thyroid hormones (THs) can modulate the immune responses through cell surface receptors. One of these is the antiviral effect of THs. Methimazole, as an anti-thyroid compound, is widely used to treat hyperthyroid patients. It also reduces blood leukocytes, granulocytes in particular, and thereby may affect the immune response. Recently, we reported that methimazole-induced hypothyroidism intensifies herpes simplex virus-1(HSV-1) infectivity. To determine whether the effect is mediated through alterations in circulating leukocytes, we assessed the HSV-1 infectivity and circulating leukocytes in methimazole-induced hypothyroid rat.

  Methods: Male Sprague Dawley rats received methimazole (200 μg/ml) in their drinking water for 2 weeks. Rats were then inoculated with a non-lethal single dose of HSV-1, and sacrificed 3 days later to harvest their spleen. Spleen extract was prepared, and virus yield was determined by evaluation of cytopathic effects (CPE) induced by the extract in a Vero cell culture system. For quantitative analysis, standard method of Reed-Muench was employed. The routine Wright’s staining protocol was used for blood leukocytes differential count.

  Results: The CPE development was significantly increased in the cell cultures exposed to the spleen extract of methimazole-treated animals (P < 0.05), indicating a higher virus yield and intensified virus infectivity. However, the effect of methimazole on blood leukocytes was minimal.

  Conclusion: Our data suggest that methimazole increases the susceptibility to HSV-1 infection, at least in part, by blocking THs synthesis but not alterations in circulating leukocytes.

Keywords: Thyroid Hormones: Methimazole, Blood Leukocytes, HSV-1, Rat
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Type of Study: article |
Received: 2014/10/16 | Accepted: 2015/01/17 | Published: 2015/03/18
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Feizi H, Moattari A, Amirghofran Z, Varedi M. Methimazole-Induced Hypothyroidism Enhances HSV-1 Infectivity without Altering Circulating Leukocytes in the Rat. J Ardabil Univ Med Sci. 2015; 15 (1) :56-65
URL: http://jarums.arums.ac.ir/article-1-781-en.html


Volume 15, Issue 1 (spring 2015) Back to browse issues page
مجله دانشگاه علوم پزشکی اردبیل Journal of Ardabil University of Medical Sciences
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