[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
:: Volume 7, Issue 4 (Winter 2007) ::
J Ardabil Univ Med Sci 2007, 7(4): 344-352 Back to browse issues page
Immunophenotyping Analysis of Tumor Infiltrating T Lymphocytes in Patients with Breast Cancer
Davar Amani , Mohammadhasan Zahir, Mojtaba Karimzadeh, Sadegh Feizollah Zadeh, Mohammadhosein Dehghan
Abstract:   (5632 Views)

 Background & Objective: Tumor-infiltrating lymphocytes (TILs) develop as recognition and defense against malignant cells by the host immune system. T cells are the most tumor infiltrating immune cells. There are controversial data about intratumor T cells and many have proposed diverse mechanisms for dysfunction of TILs. The aim of this study is analyzing Tumor Infiltrating T lymphocytes in patients with breast cancer by immunophenotyping.

 Methods: Sixteen women suffering from breast cancer were examined thirteen of them were confirmed histologically to be invasive ductal carcinoma (IDC). Tissue samples from patients and matched control group were processed for analysis by flow cytometry.

 Results: Results indicated that human breast cancer contain variable numbers of TILs. No significant changes in the percent of intratumor CD45+, CD3+ and CD3+/CD45+ cells were observed between studied group. Also there were no significant differences between cancer patients (group 1 and 2) and control group in the case of infiltration and activation status of T cells subpopulations. CD4+ cells in IDC patients and CD8+ cells in patients with other tumors (ILC+AMC) were the most infiltrated TILs. Intratumor TCD8+ cells expressed HLA-DR markers significantly more than CD25 as activation marker. In this investigation we could not find any correlation between TIL and both size and clinical stages of tumor.

 Conclusion: An immune infiltrate is an invariable finding in breast cancers, with considering the activation marker expression, TIL may be activated, albeit partially. Understanding the insensitive and/or suppressive nature of cancer cells to the immune system may provide important insights into tumor escape mechanisms as well as the development of anti-cancer strategies.

Keywords: Breast cancer, Tumor Infiltrating Lymphocytes, T cells
Full-Text [PDF 176 kb]   (1725 Downloads)    
Type of Study: article | Subject: Special
Received: 2006/09/2 | Accepted: 2006/12/28 | Published: 2008/09/14
Send email to the article author

Add your comments about this article
Your username or Email:

CAPTCHA


XML   Persian Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Amani D, Zahir M, Karimzadeh M, Feizollah Zadeh S, Dehghan M. Immunophenotyping Analysis of Tumor Infiltrating T Lymphocytes in Patients with Breast Cancer. J Ardabil Univ Med Sci. 2007; 7 (4) :344-352
URL: http://jarums.arums.ac.ir/article-1-353-en.html


Volume 7, Issue 4 (Winter 2007) Back to browse issues page
مجله دانشگاه علوم پزشکی اردبیل Journal of Ardabil University of Medical Sciences
Persian site map - English site map - Created in 0.1 seconds with 32 queries by YEKTAWEB 4122