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:: Volume 9, Issue 4 (winter 2009) ::
J Ardabil Univ Med Sci 2009, 9(4): 353-359 Back to browse issues page
Evaluation of Patients Sera for Early Diagnosis of Leptospirosis by PCR
Hamidreza Honarmand , Mohammadreza Khoramizadeh, Saeid Eshraghi
Abstract:   (4073 Views)

  Background and objectives: Leptospirosis is a very common zoonosis in the world. Early diagnosis of leptospirosis is critical because just early treatment will be effective. It's culture is very slow and serological assays are not applicable because of lack of antibodies in the first week of the disease, therefore PCR is the only option for the early diagnosis. In this study, sensitivity and accuracy of a non-quantitative conventional PCR for diagnosis of leptospirose in first week sera of patients, is evaluated

  Methods : Seventy first week sera sample of patients with clinical diagnosis of leptospirosis which were negative in MAT but positive for the second time after one weeks (seroconversion) were selected and studied.

  Results : We observed twenty four positive sera in PCR test. Sensitivity of the test was 74.5% and accuracy was 100 bacteria /ml.

  Conclusion : Result of our study shows that PCR is the only choice for the early diagnosis of Leptospirosis while other assays are not applicable but its sensitivity is low.

Keywords: Leptospirosis, Sera, Diagnosis, PCR
Full-Text [PDF 201 kb]   (1086 Downloads)    
Type of Study: article | Subject: Special
Received: 2008/05/4 | Accepted: 2010/01/12 | Published: 2010/03/18
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Honarmand H, Khoramizadeh M, Eshraghi S. Evaluation of Patients Sera for Early Diagnosis of Leptospirosis by PCR. J Ardabil Univ Med Sci. 2009; 9 (4) :353-359
URL: http://jarums.arums.ac.ir/article-1-248-en.html

Volume 9, Issue 4 (winter 2009) Back to browse issues page
مجله دانشگاه علوم پزشکی اردبیل Journal of Ardabil University of Medical Sciences
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